Cleavage stage porcine embryos may have differing developmental requirements for karyopherins α2 and α3
Identifieur interne : 001B04 ( Istex/Corpus ); précédent : 001B03; suivant : 001B05Cleavage stage porcine embryos may have differing developmental requirements for karyopherins α2 and α3
Auteurs : Ryan A. Cabot ; Randall S. PratherSource :
- Molecular Reproduction and Development [ 1040-452X ] ; 2003-03.
Abstract
Numerous cellular proteins are able to localize to the nucleus due to the fact that they possess a nuclear localization signal (NLS) in their amino acid sequence. Nuclear localization sequences recognized by the importin α/β heterodimer are found in cellular proteins capable of performing many diverse functions, ranging from chromatin remodeling to cell cycle regulation. Evidence has been presented that suggests individual importin α homologues are present at varying levels in different adult tissues. Other data have shown that specific subsets of NLSs found in different cellular proteins are recognized by individual importin α homologues with varying affinities. This evidence led us to hypothesize that due to the specific cargoes they carry, the mammalian embryo has different developmental requirements for individual importin α homologues. The results of the studies presented here indicate that importin α/β‐mediated import occurs throughout early cleavage in the porcine embryo, as determined by a reporter protein microinjection assay, and that multiple importin α homologues are present throughout early cleavage, as determined by immunocytochemical analysis. An RNA interference approach was used in an attempt to determine the developmental requirements for specific importin α homologues during early cleavage in the porcine embryo. Results from this study showed that fertilized porcine embryos injected with double stranded RNA (dsRNA) corresponding to the importin α homologue karyopherin α3 had significantly fewer nuclei following four days of culture than did embryos injected with dsRNA for another importin α homologue, karyopherin α2, or two control groups. This is the first report indicating that mammalian embryos may have differential developmental requirements for specific nuclear trafficking pathways. Mol. Reprod. Dev. 64: 292–301, 2003. © 2003 Wiley‐Liss, Inc.
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DOI: 10.1002/mrd.10238
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Results from this study showed that fertilized porcine embryos injected with double stranded RNA (dsRNA) corresponding to the importin α homologue karyopherin α3 had significantly fewer nuclei following four days of culture than did embryos injected with dsRNA for another importin α homologue, karyopherin α2, or two control groups. This is the first report indicating that mammalian embryos may have differential developmental requirements for specific nuclear trafficking pathways. Mol. Reprod. Dev. 64: 292–301, 2003. © 2003 Wiley‐Liss, Inc.</p></abstract><textClass><keywords xml:lang="en"><term xml:id="kwd1">developmental biology</term><term xml:id="kwd2">embryo</term><term xml:id="kwd3">early development</term><term xml:id="kwd4">in vitro fertilization</term></keywords><keywords rend="articleCategory"><term>Research Article</term></keywords><keywords rend="tocHeading1"><term>Embryo Development</term></keywords></textClass><langUsage><language ident="en"></language></langUsage></profileDesc><revisionDesc><change when="2019-12-20" who="#istex" xml:id="pub2tei">formatting</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-0KVK89VR-G/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Wiley Subscription Services, Inc., A Wiley Company</publisherName><publisherLoc>New York</publisherLoc></publisherInfo><doi registered="yes">10.1002/(ISSN)1098-2795</doi><issn type="print">1040-452X</issn><issn type="electronic">1098-2795</issn><idGroup><id type="product" value="MRD"></id></idGroup><titleGroup><title type="main" xml:lang="en" sort="MOLECULAR REPRODUCTION AND DEVELOPMENT">Molecular Reproduction and Development</title><title type="short">Mol. Reprod. Dev.</title></titleGroup></publicationMeta><publicationMeta level="part" position="30"><doi origin="wiley" registered="yes">10.1002/mrd.v64:3</doi><numberingGroup><numbering type="journalVolume" number="64">64</numbering><numbering type="journalIssue">3</numbering></numberingGroup><coverDate startDate="2003-03">March 2003</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="6" status="forIssue"><doi origin="wiley" registered="yes">10.1002/mrd.10238</doi><idGroup><id type="unit" value="MRD10238"></id></idGroup><countGroup><count type="pageTotal" number="10"></count></countGroup><titleGroup><title type="articleCategory">Research Article</title><title type="tocHeading1">Embryo Development</title></titleGroup><copyright ownership="publisher">Copyright © 2003 Wiley‐Liss, Inc.</copyright><eventGroup><event type="manuscriptReceived" date="2002-07-19"></event><event type="manuscriptAccepted" date="2002-10-30"></event><event type="firstOnline" date="2003-01-22"></event><event type="publishedOnlineFinalForm" date="2003-01-22"></event><event type="xmlConverted" agent="Converter:JWSART34_TO_WML3G version:2.3.18.1 mode:FullText source:HeaderRef result:HeaderRef" date="2010-09-09"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-02-03"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-31"></event></eventGroup><numberingGroup><numbering type="pageFirst">292</numbering><numbering type="pageLast">301</numbering></numberingGroup><correspondenceTo>Department of Animal Sciences, University of Missouri, 162 Animal Science Research Center, Columbia, MI 65211.</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:MRD.MRD10238.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="4"></count><count type="tableTotal" number="1"></count><count type="referenceTotal" number="46"></count><count type="wordTotal" number="1283"></count></countGroup><titleGroup><title type="main" xml:lang="en">Cleavage stage porcine embryos may have differing developmental requirements for karyopherins α2 and α3</title><title type="short" xml:lang="en">KARYOPHERINS α2 AND α3 IN THE PORCINE EMBRYO</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Ryan A.</givenNames><familyName>Cabot</familyName></personName></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#af1" corresponding="yes"><personName><givenNames>Randall S.</givenNames><familyName>Prather</familyName></personName><contactDetails><email>PratherR@missouri.edu</email></contactDetails></creator></creators><affiliationGroup><affiliation xml:id="af1" countryCode="US" type="organization"><unparsedAffiliation>Department of Animal Sciences, University of Missouri, 162 Animal Science Research Center, Columbia, Missouri</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">developmental biology</keyword><keyword xml:id="kwd2">embryo</keyword><keyword xml:id="kwd3">early development</keyword><keyword xml:id="kwd4">in vitro fertilization</keyword></keywordGroup><fundingInfo><fundingAgency>F21C group at UMC</fundingAgency></fundingInfo><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Numerous cellular proteins are able to localize to the nucleus due to the fact that they possess a nuclear localization signal (NLS) in their amino acid sequence. Nuclear localization sequences recognized by the importin α/β heterodimer are found in cellular proteins capable of performing many diverse functions, ranging from chromatin remodeling to cell cycle regulation. Evidence has been presented that suggests individual importin α homologues are present at varying levels in different adult tissues. Other data have shown that specific subsets of NLSs found in different cellular proteins are recognized by individual importin α homologues with varying affinities. This evidence led us to hypothesize that due to the specific cargoes they carry, the mammalian embryo has different developmental requirements for individual importin α homologues. The results of the studies presented here indicate that importin α/β‐mediated import occurs throughout early cleavage in the porcine embryo, as determined by a reporter protein microinjection assay, and that multiple importin α homologues are present throughout early cleavage, as determined by immunocytochemical analysis. An RNA interference approach was used in an attempt to determine the developmental requirements for specific importin α homologues during early cleavage in the porcine embryo. Results from this study showed that fertilized porcine embryos injected with double stranded RNA (dsRNA) corresponding to the importin α homologue karyopherin α3 had significantly fewer nuclei following four days of culture than did embryos injected with dsRNA for another importin α homologue, karyopherin α2, or two control groups. This is the first report indicating that mammalian embryos may have differential developmental requirements for specific nuclear trafficking pathways. Mol. Reprod. Dev. 64: 292–301, 2003. © 2003 Wiley‐Liss, Inc.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Cleavage stage porcine embryos may have differing developmental requirements for karyopherins α2 and α3</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>KARYOPHERINS α2 AND α3 IN THE PORCINE EMBRYO</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Cleavage stage porcine embryos may have differing developmental requirements for karyopherins α2 and α3</title></titleInfo><name type="personal"><namePart type="given">Ryan A.</namePart><namePart type="family">Cabot</namePart><affiliation>Department of Animal Sciences, University of Missouri, 162 Animal Science Research Center, Columbia, Missouri</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Randall S.</namePart><namePart type="family">Prather</namePart><affiliation>Department of Animal Sciences, University of Missouri, 162 Animal Science Research Center, Columbia, Missouri</affiliation><affiliation>E-mail: PratherR@missouri.edu</affiliation><affiliation>Correspondence address: Department of Animal Sciences, University of Missouri, 162 Animal Science Research Center, Columbia, MI 65211.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><place><placeTerm type="text">New York</placeTerm></place><dateIssued encoding="w3cdtf">2003-03</dateIssued><dateCaptured encoding="w3cdtf">2002-07-19</dateCaptured><dateValid encoding="w3cdtf">2002-10-30</dateValid><copyrightDate encoding="w3cdtf">2003</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><extent unit="figures">4</extent><extent unit="tables">1</extent><extent unit="references">46</extent><extent unit="words">1283</extent></physicalDescription><abstract lang="en">Numerous cellular proteins are able to localize to the nucleus due to the fact that they possess a nuclear localization signal (NLS) in their amino acid sequence. Nuclear localization sequences recognized by the importin α/β heterodimer are found in cellular proteins capable of performing many diverse functions, ranging from chromatin remodeling to cell cycle regulation. Evidence has been presented that suggests individual importin α homologues are present at varying levels in different adult tissues. Other data have shown that specific subsets of NLSs found in different cellular proteins are recognized by individual importin α homologues with varying affinities. This evidence led us to hypothesize that due to the specific cargoes they carry, the mammalian embryo has different developmental requirements for individual importin α homologues. The results of the studies presented here indicate that importin α/β‐mediated import occurs throughout early cleavage in the porcine embryo, as determined by a reporter protein microinjection assay, and that multiple importin α homologues are present throughout early cleavage, as determined by immunocytochemical analysis. An RNA interference approach was used in an attempt to determine the developmental requirements for specific importin α homologues during early cleavage in the porcine embryo. Results from this study showed that fertilized porcine embryos injected with double stranded RNA (dsRNA) corresponding to the importin α homologue karyopherin α3 had significantly fewer nuclei following four days of culture than did embryos injected with dsRNA for another importin α homologue, karyopherin α2, or two control groups. This is the first report indicating that mammalian embryos may have differential developmental requirements for specific nuclear trafficking pathways. Mol. Reprod. 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