Systematic characterization of protein-DNA interactions
Identifieur interne : 001980 ( Istex/Corpus ); précédent : 001979; suivant : 001981Systematic characterization of protein-DNA interactions
Auteurs : Zhi Xie ; Shaohui Hu ; Jiang Qian ; Seth Blackshaw ; Heng ZhuSource :
- Cellular and Molecular Life Sciences [ 1420-682X ] ; 2011-05-01.
English descriptors
- KwdEn :
Abstract
Abstract: Sequence-specific protein-DNA interactions (PDIs) are critical for regulating many cellular processes, including transcription, DNA replication, repair, and rearrangement. We review recent experimental advances in high-throughput technologies designed to characterize PDIs and discuss recent studies that use these tools, including ChIP-chip/seq, SELEX-based approaches, yeast one-hybrid, bacterial one-hybrid, protein binding microarray, and protein microarray. The results of these studies have challenged some long-standing concepts of PDI and provide valuable insights into the complex transcriptional regulatory networks.
Url:
DOI: 10.1007/s00018-010-0617-y
Links to Exploration step
ISTEX:35A7CA9912BAC9BE7F6EFDEFDE6EA2A8D886C2AALe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Systematic characterization of protein-DNA interactions</title><author><name sortKey="Xie, Zhi" sort="Xie, Zhi" uniqKey="Xie Z" first="Zhi" last="Xie">Zhi Xie</name><affiliation><mods:affiliation>Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>The Center for Human Immunology, National Institutes of Health, Bethesda, MD, USA</mods:affiliation></affiliation></author><author><name sortKey="Hu, Shaohui" sort="Hu, Shaohui" uniqKey="Hu S" first="Shaohui" last="Hu">Shaohui Hu</name><affiliation><mods:affiliation>The Center for High-Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA</mods:affiliation></affiliation></author><author><name sortKey="Qian, Jiang" sort="Qian, Jiang" uniqKey="Qian J" first="Jiang" last="Qian">Jiang Qian</name><affiliation><mods:affiliation>Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA</mods:affiliation></affiliation></author><author><name sortKey="Blackshaw, Seth" sort="Blackshaw, Seth" uniqKey="Blackshaw S" first="Seth" last="Blackshaw">Seth Blackshaw</name><affiliation><mods:affiliation>The Center for High-Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA</mods:affiliation></affiliation></author><author><name sortKey="Zhu, Heng" sort="Zhu, Heng" uniqKey="Zhu H" first="Heng" last="Zhu">Heng Zhu</name><affiliation><mods:affiliation>The Center for High-Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>Room 333, BRB, 733 N. Broadway, 21205, Baltimore, MD, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: heng.zhu@jhmi.edu</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:35A7CA9912BAC9BE7F6EFDEFDE6EA2A8D886C2AA</idno><date when="2010" year="2010">2010</date><idno type="doi">10.1007/s00018-010-0617-y</idno><idno type="url">https://api.istex.fr/ark:/67375/VQC-75B6BHLM-9/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001980</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001980</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Systematic characterization of protein-DNA interactions</title><author><name sortKey="Xie, Zhi" sort="Xie, Zhi" uniqKey="Xie Z" first="Zhi" last="Xie">Zhi Xie</name><affiliation><mods:affiliation>Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>The Center for Human Immunology, National Institutes of Health, Bethesda, MD, USA</mods:affiliation></affiliation></author><author><name sortKey="Hu, Shaohui" sort="Hu, Shaohui" uniqKey="Hu S" first="Shaohui" last="Hu">Shaohui Hu</name><affiliation><mods:affiliation>The Center for High-Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA</mods:affiliation></affiliation></author><author><name sortKey="Qian, Jiang" sort="Qian, Jiang" uniqKey="Qian J" first="Jiang" last="Qian">Jiang Qian</name><affiliation><mods:affiliation>Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA</mods:affiliation></affiliation></author><author><name sortKey="Blackshaw, Seth" sort="Blackshaw, Seth" uniqKey="Blackshaw S" first="Seth" last="Blackshaw">Seth Blackshaw</name><affiliation><mods:affiliation>The Center for High-Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA</mods:affiliation></affiliation></author><author><name sortKey="Zhu, Heng" sort="Zhu, Heng" uniqKey="Zhu H" first="Heng" last="Zhu">Heng Zhu</name><affiliation><mods:affiliation>The Center for High-Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>Room 333, BRB, 733 N. Broadway, 21205, Baltimore, MD, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: heng.zhu@jhmi.edu</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Cellular and Molecular Life Sciences</title><title level="j" type="abbrev">Cell. Mol. Life Sci.</title><idno type="ISSN">1420-682X</idno><idno type="eISSN">1420-9071</idno><imprint><publisher>SP Birkhäuser Verlag Basel</publisher><pubPlace>Basel</pubPlace><date type="published" when="2011-05-01">2011-05-01</date><biblScope unit="volume">68</biblScope><biblScope unit="issue">10</biblScope><biblScope unit="page" from="1657">1657</biblScope><biblScope unit="page" to="1668">1668</biblScope></imprint><idno type="ISSN">1420-682X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1420-682X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA-binding specificity</term><term>Protein DNA interactions</term><term>Protein microarray</term><term>Transcription factors</term><term>Unconventional DNA-binding proteins</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Sequence-specific protein-DNA interactions (PDIs) are critical for regulating many cellular processes, including transcription, DNA replication, repair, and rearrangement. We review recent experimental advances in high-throughput technologies designed to characterize PDIs and discuss recent studies that use these tools, including ChIP-chip/seq, SELEX-based approaches, yeast one-hybrid, bacterial one-hybrid, protein binding microarray, and protein microarray. The results of these studies have challenged some long-standing concepts of PDI and provide valuable insights into the complex transcriptional regulatory networks.</div></front></TEI><istex><corpusName>springer-journals</corpusName><author><json:item><name>Zhi Xie</name><affiliations><json:string>Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA</json:string><json:string>The Center for Human Immunology, National Institutes of Health, Bethesda, MD, USA</json:string></affiliations></json:item><json:item><name>Shaohui Hu</name><affiliations><json:string>The Center for High-Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA</json:string><json:string>Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA</json:string></affiliations></json:item><json:item><name>Jiang Qian</name><affiliations><json:string>Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA</json:string><json:string>The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA</json:string></affiliations></json:item><json:item><name>Seth Blackshaw</name><affiliations><json:string>The Center for High-Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA</json:string><json:string>The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA</json:string></affiliations></json:item><json:item><name>Heng Zhu</name><affiliations><json:string>The Center for High-Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA</json:string><json:string>Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA</json:string><json:string>The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA</json:string><json:string>Room 333, BRB, 733 N. Broadway, 21205, Baltimore, MD, USA</json:string><json:string>E-mail: heng.zhu@jhmi.edu</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Protein DNA interactions</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Transcription factors</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Unconventional DNA-binding proteins</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Protein microarray</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>DNA-binding specificity</value></json:item></subject><articleId><json:string>617</json:string><json:string>s00018-010-0617-y</json:string></articleId><arkIstex>ark:/67375/VQC-75B6BHLM-9</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>ReviewPaper</json:string></originalGenre><abstract>Abstract: Sequence-specific protein-DNA interactions (PDIs) are critical for regulating many cellular processes, including transcription, DNA replication, repair, and rearrangement. We review recent experimental advances in high-throughput technologies designed to characterize PDIs and discuss recent studies that use these tools, including ChIP-chip/seq, SELEX-based approaches, yeast one-hybrid, bacterial one-hybrid, protein binding microarray, and protein microarray. The results of these studies have challenged some long-standing concepts of PDI and provide valuable insights into the complex transcriptional regulatory networks.</abstract><qualityIndicators><refBibsNative>false</refBibsNative><abstractWordCount>75</abstractWordCount><abstractCharCount>636</abstractCharCount><keywordCount>5</keywordCount><score>7.9</score><pdfWordCount>7423</pdfWordCount><pdfCharCount>48919</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>12</pdfPageCount><pdfPageSize>595.276 x 790.866 pts</pdfPageSize></qualityIndicators><title>Systematic characterization of protein-DNA interactions</title><genre><json:string>review-article</json:string></genre><host><title>Cellular and Molecular Life Sciences</title><language><json:string>unknown</json:string></language><publicationDate>2011</publicationDate><copyrightDate>2011</copyrightDate><issn><json:string>1420-682X</json:string></issn><eissn><json:string>1420-9071</json:string></eissn><journalId><json:string>18</json:string></journalId><volume>68</volume><issue>10</issue><pages><first>1657</first><last>1668</last></pages><genre><json:string>journal</json:string></genre><subject><json:item><value>Biochemistry, general</value></json:item><json:item><value>Life Sciences, general</value></json:item><json:item><value>Biomedicine general</value></json:item><json:item><value>Cell Biology</value></json:item></subject></host><ark><json:string>ark:/67375/VQC-75B6BHLM-9</json:string></ark><publicationDate>2011</publicationDate><copyrightDate>2010</copyrightDate><doi><json:string>10.1007/s00018-010-0617-y</json:string></doi><id>35A7CA9912BAC9BE7F6EFDEFDE6EA2A8D886C2AA</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-75B6BHLM-9/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-75B6BHLM-9/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/VQC-75B6BHLM-9/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Systematic characterization of protein-DNA interactions</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">SP Birkhäuser Verlag Basel</publisher><pubPlace>Basel</pubPlace><availability><licence><p>Springer Basel AG, 2010</p></licence><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</p></availability><date>2010-09-09</date></publicationStmt><notesStmt><note type="review-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-L5L7X3NF-P">review-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note>Review</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Systematic characterization of protein-DNA interactions</title><author xml:id="author-0000"><persName><forename type="first">Zhi</forename><surname>Xie</surname></persName><affiliation>Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA</affiliation><affiliation>The Center for Human Immunology, National Institutes of Health, Bethesda, MD, USA</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Shaohui</forename><surname>Hu</surname></persName><affiliation>The Center for High-Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA</affiliation><affiliation>Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Jiang</forename><surname>Qian</surname></persName><affiliation>Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA</affiliation><affiliation>The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Seth</forename><surname>Blackshaw</surname></persName><affiliation>The Center for High-Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA</affiliation><affiliation>The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA</affiliation></author><author xml:id="author-0004" corresp="yes"><persName><forename type="first">Heng</forename><surname>Zhu</surname></persName><email>heng.zhu@jhmi.edu</email><affiliation>The Center for High-Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA</affiliation><affiliation>Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA</affiliation><affiliation>The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA</affiliation><affiliation>Room 333, BRB, 733 N. Broadway, 21205, Baltimore, MD, USA</affiliation></author><idno type="istex">35A7CA9912BAC9BE7F6EFDEFDE6EA2A8D886C2AA</idno><idno type="ark">ark:/67375/VQC-75B6BHLM-9</idno><idno type="DOI">10.1007/s00018-010-0617-y</idno><idno type="article-id">617</idno><idno type="article-id">s00018-010-0617-y</idno></analytic><monogr><title level="j">Cellular and Molecular Life Sciences</title><title level="j" type="abbrev">Cell. Mol. Life Sci.</title><idno type="pISSN">1420-682X</idno><idno type="eISSN">1420-9071</idno><idno type="journal-ID">true</idno><idno type="issue-article-count">12</idno><idno type="volume-issue-count">24</idno><imprint><publisher>SP Birkhäuser Verlag Basel</publisher><pubPlace>Basel</pubPlace><date type="published" when="2011-05-01"></date><biblScope unit="volume">68</biblScope><biblScope unit="issue">10</biblScope><biblScope unit="page" from="1657">1657</biblScope><biblScope unit="page" to="1668">1668</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2010-09-09</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Sequence-specific protein-DNA interactions (PDIs) are critical for regulating many cellular processes, including transcription, DNA replication, repair, and rearrangement. We review recent experimental advances in high-throughput technologies designed to characterize PDIs and discuss recent studies that use these tools, including ChIP-chip/seq, SELEX-based approaches, yeast one-hybrid, bacterial one-hybrid, protein binding microarray, and protein microarray. The results of these studies have challenged some long-standing concepts of PDI and provide valuable insights into the complex transcriptional regulatory networks.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Protein DNA interactions</term></item><item><term>Transcription factors</term></item><item><term>Unconventional DNA-binding proteins</term></item><item><term>Protein microarray</term></item><item><term>DNA-binding specificity</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>Life Sciences</head><item><term>Biochemistry, general</term></item><item><term>Life Sciences, general</term></item><item><term>Biomedicine general</term></item><item><term>Cell Biology</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2010-09-09">Created</change><change when="2011-05-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-75B6BHLM-9/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus springer-journals not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" name="istex:docType"></istex:docType><istex:document><Publisher><PublisherInfo><PublisherName>SP Birkhäuser Verlag Basel</PublisherName><PublisherLocation>Basel</PublisherLocation></PublisherInfo><Journal OutputMedium="All"><JournalInfo JournalProductType="NonStandardArchiveJournal" NumberingStyle="Unnumbered"><JournalID>18</JournalID><JournalPrintISSN>1420-682X</JournalPrintISSN><JournalElectronicISSN>1420-9071</JournalElectronicISSN><JournalTitle>Cellular and Molecular Life Sciences</JournalTitle><JournalAbbreviatedTitle>Cell. Mol. Life Sci.</JournalAbbreviatedTitle><JournalSubjectGroup><JournalSubject Type="Primary">Life Sciences</JournalSubject><JournalSubject Type="Secondary">Biochemistry, general</JournalSubject><JournalSubject Type="Secondary">Life Sciences, general </JournalSubject><JournalSubject Type="Secondary">Biomedicine general</JournalSubject><JournalSubject Type="Secondary">Cell Biology</JournalSubject></JournalSubjectGroup></JournalInfo><Volume OutputMedium="All"><VolumeInfo TocLevels="0" VolumeType="Regular"><VolumeIDStart>68</VolumeIDStart><VolumeIDEnd>68</VolumeIDEnd><VolumeIssueCount>24</VolumeIssueCount></VolumeInfo><Issue IssueType="Regular" OutputMedium="All"><IssueInfo IssueType="Regular" TocLevels="0"><IssueIDStart>10</IssueIDStart><IssueIDEnd>10</IssueIDEnd><IssueArticleCount>12</IssueArticleCount><IssueHistory><OnlineDate><Year>2011</Year><Month>4</Month><Day>16</Day></OnlineDate><PrintDate><Year>2011</Year><Month>4</Month><Day>15</Day></PrintDate><CoverDate><Year>2011</Year><Month>5</Month></CoverDate><PricelistYear>2011</PricelistYear></IssueHistory><IssueCopyright><CopyrightHolderName>Springer Basel AG</CopyrightHolderName><CopyrightYear>2011</CopyrightYear></IssueCopyright></IssueInfo><Article ID="s00018-010-0617-y" OutputMedium="All"><ArticleInfo ArticleType="ReviewPaper" ContainsESM="No" Language="En" NumberingStyle="Unnumbered" TocLevels="0"><ArticleID>617</ArticleID><ArticleDOI>10.1007/s00018-010-0617-y</ArticleDOI><ArticleSequenceNumber>2</ArticleSequenceNumber><ArticleTitle Language="En" OutputMedium="All">Systematic characterization of protein-DNA interactions</ArticleTitle><ArticleCategory>Review</ArticleCategory><ArticleFirstPage>1657</ArticleFirstPage><ArticleLastPage>1668</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2010</Year><Month>12</Month><Day>17</Day></RegistrationDate><Received><Year>2010</Year><Month>9</Month><Day>9</Day></Received><Revised><Year>2010</Year><Month>11</Month><Day>29</Day></Revised><Accepted><Year>2010</Year><Month>12</Month><Day>16</Day></Accepted><OnlineDate><Year>2011</Year><Month>1</Month><Day>5</Day></OnlineDate></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer Basel AG</CopyrightHolderName><CopyrightYear>2010</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1 Aff6" PresentAffiliationID="Aff6"><AuthorName DisplayOrder="Western"><GivenName>Zhi</GivenName><FamilyName>Xie</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2 Aff3"><AuthorName DisplayOrder="Western"><GivenName>Shaohui</GivenName><FamilyName>Hu</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff5"><AuthorName DisplayOrder="Western"><GivenName>Jiang</GivenName><FamilyName>Qian</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2 Aff4"><AuthorName DisplayOrder="Western"><GivenName>Seth</GivenName><FamilyName>Blackshaw</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2 Aff3 Aff5 Aff7" CorrespondingAffiliationID="Aff7"><AuthorName DisplayOrder="Western"><GivenName>Heng</GivenName><FamilyName>Zhu</FamilyName></AuthorName><Contact><Phone>+1-410-5020878</Phone><Fax>+1-410-5021872</Fax><Email>heng.zhu@jhmi.edu</Email></Contact></Author><Affiliation ID="Aff1"><OrgDivision>Department of Ophthalmology</OrgDivision><OrgName>Johns Hopkins University School of Medicine</OrgName><OrgAddress><City>Baltimore</City><State>MD</State><Country Code="US">USA</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>The Center for High-Throughput Biology</OrgDivision><OrgName>Johns Hopkins University School of Medicine</OrgName><OrgAddress><City>Baltimore</City><State>MD</State><Country Code="US">USA</Country></OrgAddress></Affiliation><Affiliation ID="Aff3"><OrgDivision>Department of Pharmacology and Molecular Sciences</OrgDivision><OrgName>Johns Hopkins University School of Medicine</OrgName><OrgAddress><City>Baltimore</City><State>MD</State><Country Code="US">USA</Country></OrgAddress></Affiliation><Affiliation ID="Aff4"><OrgDivision>The Solomon H. Snyder Department of Neuroscience</OrgDivision><OrgName>Johns Hopkins University School of Medicine</OrgName><OrgAddress><City>Baltimore</City><State>MD</State><Country Code="US">USA</Country></OrgAddress></Affiliation><Affiliation ID="Aff5"><OrgDivision>The Sidney Kimmel Comprehensive Cancer Center</OrgDivision><OrgName>Johns Hopkins University School of Medicine</OrgName><OrgAddress><City>Baltimore</City><State>MD</State><Country Code="US">USA</Country></OrgAddress></Affiliation><Affiliation ID="Aff6"><OrgDivision>The Center for Human Immunology</OrgDivision><OrgName>National Institutes of Health</OrgName><OrgAddress><City>Bethesda</City><State>MD</State><Country Code="US">USA</Country></OrgAddress></Affiliation><Affiliation ID="Aff7"><OrgAddress><Street>Room 333, BRB, 733 N. Broadway</Street><Postcode>21205</Postcode><City>Baltimore</City><State>MD</State><Country Code="US">USA</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En" OutputMedium="All"><Heading>Abstract</Heading><Para>Sequence-specific protein-DNA interactions (PDIs) are critical for regulating many cellular processes, including transcription, DNA replication, repair, and rearrangement. We review recent experimental advances in high-throughput technologies designed to characterize PDIs and discuss recent studies that use these tools, including ChIP-chip/seq, SELEX-based approaches, yeast one-hybrid, bacterial one-hybrid, protein binding microarray, and protein microarray. The results of these studies have challenged some long-standing concepts of PDI and provide valuable insights into the complex transcriptional regulatory networks.</Para></Abstract><KeywordGroup Language="En" OutputMedium="All"><Heading>Keywords</Heading><Keyword>Protein DNA interactions</Keyword><Keyword>Transcription factors</Keyword><Keyword>Unconventional DNA-binding proteins</Keyword><Keyword>Protein microarray</Keyword><Keyword>DNA-binding specificity</Keyword></KeywordGroup></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Systematic characterization of protein-DNA interactions</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Systematic characterization of protein-DNA interactions</title></titleInfo><name type="personal"><namePart type="given">Zhi</namePart><namePart type="family">Xie</namePart><affiliation>Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA</affiliation><affiliation>The Center for Human Immunology, National Institutes of Health, Bethesda, MD, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Shaohui</namePart><namePart type="family">Hu</namePart><affiliation>The Center for High-Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA</affiliation><affiliation>Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jiang</namePart><namePart type="family">Qian</namePart><affiliation>Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA</affiliation><affiliation>The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Seth</namePart><namePart type="family">Blackshaw</namePart><affiliation>The Center for High-Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA</affiliation><affiliation>The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Heng</namePart><namePart type="family">Zhu</namePart><affiliation>The Center for High-Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA</affiliation><affiliation>Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA</affiliation><affiliation>The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA</affiliation><affiliation>Room 333, BRB, 733 N. Broadway, 21205, Baltimore, MD, USA</affiliation><affiliation>E-mail: heng.zhu@jhmi.edu</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="review-article" displayLabel="ReviewPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-L5L7X3NF-P">review-article</genre><originInfo><publisher>SP Birkhäuser Verlag Basel</publisher><place><placeTerm type="text">Basel</placeTerm></place><dateCreated encoding="w3cdtf">2010-09-09</dateCreated><dateIssued encoding="w3cdtf">2011-05-01</dateIssued><copyrightDate encoding="w3cdtf">2010</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: Sequence-specific protein-DNA interactions (PDIs) are critical for regulating many cellular processes, including transcription, DNA replication, repair, and rearrangement. We review recent experimental advances in high-throughput technologies designed to characterize PDIs and discuss recent studies that use these tools, including ChIP-chip/seq, SELEX-based approaches, yeast one-hybrid, bacterial one-hybrid, protein binding microarray, and protein microarray. The results of these studies have challenged some long-standing concepts of PDI and provide valuable insights into the complex transcriptional regulatory networks.</abstract><note>Review</note><subject lang="en"><genre>Keywords</genre><topic>Protein DNA interactions</topic><topic>Transcription factors</topic><topic>Unconventional DNA-binding proteins</topic><topic>Protein microarray</topic><topic>DNA-binding specificity</topic></subject><relatedItem type="host"><titleInfo><title>Cellular and Molecular Life Sciences</title></titleInfo><titleInfo type="abbreviated"><title>Cell. Mol. Life Sci.</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">2011-04-16</dateIssued><copyrightDate encoding="w3cdtf">2011</copyrightDate></originInfo><subject><genre>Life Sciences</genre><topic>Biochemistry, general</topic><topic>Life Sciences, general</topic><topic>Biomedicine general</topic><topic>Cell Biology</topic></subject><identifier type="ISSN">1420-682X</identifier><identifier type="eISSN">1420-9071</identifier><identifier type="JournalID">18</identifier><identifier type="IssueArticleCount">12</identifier><identifier type="VolumeIssueCount">24</identifier><part><date>2011</date><detail type="volume"><number>68</number><caption>vol.</caption></detail><detail type="issue"><number>10</number><caption>no.</caption></detail><extent unit="pages"><start>1657</start><end>1668</end></extent></part><recordInfo><recordOrigin>Springer Basel AG, 2011</recordOrigin></recordInfo></relatedItem><identifier type="istex">35A7CA9912BAC9BE7F6EFDEFDE6EA2A8D886C2AA</identifier><identifier type="ark">ark:/67375/VQC-75B6BHLM-9</identifier><identifier type="DOI">10.1007/s00018-010-0617-y</identifier><identifier type="ArticleID">617</identifier><identifier type="ArticleID">s00018-010-0617-y</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer Basel AG, 2010</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Springer Basel AG, 2010</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-75B6BHLM-9/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001980 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 001980 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:35A7CA9912BAC9BE7F6EFDEFDE6EA2A8D886C2AA
|texte= Systematic characterization of protein-DNA interactions
}}
| This area was generated with Dilib version V0.6.33. |  |