Prediction of functional regions of the maize streak virus replication-associated proteins by protein-protein interaction analysis
Identifieur interne : 001979 ( Istex/Corpus ); précédent : 001978; suivant : 001980Prediction of functional regions of the maize streak virus replication-associated proteins by protein-protein interaction analysis
Auteurs : Gábor V. Horváth ; Aladár Pettk Szandtner ; Krisztina Nikovics ; Metin Bilgin ; Margaret Boulton ; Jeffrey W. Davies ; Crisanto Gutiérrez ; Dénes DuditsSource :
- Plant Molecular Biology [ 0167-4412 ] ; 1998-11-01.
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Abstract
Abstract: The replication of the geminiviruses depends on the viral encoded early (complementary-sense) proteins and on host genome encoded factors. Additionally, some of the early proteins (the AL2 protein of subgroup III, and the RepA (formerly known as C1) or Rep (C1:C2) proteins of subgroup I geminiviruses) can function as transcriptional activators of virion- (V-)sense gene expression. The yeast two-hybrid system has allowed us to predict some of the functionally important regions of the maize streak virus (MSV) early proteins RepA and Rep. Defined domains of these proteins were shown to act as transactivators in yeast cells. We detected the association of the RepA and Rep proteins, and their subfragments, with the maize retinoblastoma protein (ZmRb1) which is likely to be one of the interacting host proteins. We showed the self-association capability of the MSV proteins and suggest that homo- or hetero-oligomerization may play an important role in virus replication. These results provide new insights into the role of different regions of the MSV proteins in relation to transcriptional activation and regulation of viral DNA replication.
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DOI: 10.1023/A:1006076316887
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ISTEX:8356C70381135F9971E4FAAA6B17D73AF0B99DB1Le document en format XML
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Box 521, 6701, Szeged, Hungary</mods:affiliation></affiliation></author><author><name sortKey="Bilgin, Metin" sort="Bilgin, Metin" uniqKey="Bilgin M" first="Metin" last="Bilgin">Metin Bilgin</name><affiliation><mods:affiliation>Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, 6701, Szeged, Hungary</mods:affiliation></affiliation></author><author><name sortKey="Boulton, Margaret" sort="Boulton, Margaret" uniqKey="Boulton M" first="Margaret" last="Boulton">Margaret Boulton</name><affiliation><mods:affiliation>John Innes Centre, Norwich Research Park, NR4 7UH, Norwich, UK</mods:affiliation></affiliation></author><author><name sortKey="Davies, Jeffrey W" sort="Davies, Jeffrey W" uniqKey="Davies J" first="Jeffrey W." last="Davies">Jeffrey W. 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Box 521, 6701, Szeged, Hungary</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Plant Molecular Biology</title><title level="j" type="sub">An International Journal on Molecular Biology, Molecular Genetics and Biochemistry</title><title level="j" type="abbrev">Plant Mol Biol</title><idno type="ISSN">0167-4412</idno><idno type="eISSN">1573-5028</idno><imprint><publisher>Kluwer Academic Publishers</publisher><pubPlace>Dordrecht</pubPlace><date type="published" when="1998-11-01">1998-11-01</date><biblScope unit="volume">38</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="699">699</biblScope><biblScope unit="page" to="712">712</biblScope></imprint><idno type="ISSN">0167-4412</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0167-4412</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Rep proteins</term><term>maize retinoblastoma protein</term><term>maize streak virus</term><term>transactivation</term><term>yeast two-hybrid system</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The replication of the geminiviruses depends on the viral encoded early (complementary-sense) proteins and on host genome encoded factors. Additionally, some of the early proteins (the AL2 protein of subgroup III, and the RepA (formerly known as C1) or Rep (C1:C2) proteins of subgroup I geminiviruses) can function as transcriptional activators of virion- (V-)sense gene expression. The yeast two-hybrid system has allowed us to predict some of the functionally important regions of the maize streak virus (MSV) early proteins RepA and Rep. Defined domains of these proteins were shown to act as transactivators in yeast cells. We detected the association of the RepA and Rep proteins, and their subfragments, with the maize retinoblastoma protein (ZmRb1) which is likely to be one of the interacting host proteins. We showed the self-association capability of the MSV proteins and suggest that homo- or hetero-oligomerization may play an important role in virus replication. These results provide new insights into the role of different regions of the MSV proteins in relation to transcriptional activation and regulation of viral DNA replication.</div></front></TEI><istex><corpusName>springer-journals</corpusName><author><json:item><name>Gábor V. Horváth</name><affiliations><json:string>Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, 6701, Szeged, Hungary</json:string></affiliations></json:item><json:item><name>Aladár Pettkó-Szandtner</name><affiliations><json:string>Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, 6701, Szeged, Hungary</json:string></affiliations></json:item><json:item><name>Krisztina Nikovics</name><affiliations><json:string>Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, P.O. 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Davies</name><affiliations><json:string>John Innes Centre, Norwich Research Park, NR4 7UH, Norwich, UK</json:string></affiliations></json:item><json:item><name>Crisanto Gutiérrez</name><affiliations><json:string>Centro de Biología Molecular ‘Severo Ochoa’ (CSIC-UAM), Universidad Autónoma, Cantoblanco, 28049, Madrid, Spain</json:string></affiliations></json:item><json:item><name>Dénes Dudits</name><affiliations><json:string>Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, P.O. 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Additionally, some of the early proteins (the AL2 protein of subgroup III, and the RepA (formerly known as C1) or Rep (C1:C2) proteins of subgroup I geminiviruses) can function as transcriptional activators of virion- (V-)sense gene expression. The yeast two-hybrid system has allowed us to predict some of the functionally important regions of the maize streak virus (MSV) early proteins RepA and Rep. Defined domains of these proteins were shown to act as transactivators in yeast cells. We detected the association of the RepA and Rep proteins, and their subfragments, with the maize retinoblastoma protein (ZmRb1) which is likely to be one of the interacting host proteins. We showed the self-association capability of the MSV proteins and suggest that homo- or hetero-oligomerization may play an important role in virus replication. 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Additionally, some of the early proteins (the AL2 protein of subgroup III, and the RepA (formerly known as C1) or Rep (C1:C2) proteins of subgroup I geminiviruses) can function as transcriptional activators of virion- (V-)sense gene expression. The yeast two-hybrid system has allowed us to predict some of the functionally important regions of the maize streak virus (MSV) early proteins RepA and Rep. Defined domains of these proteins were shown to act as transactivators in yeast cells. We detected the association of the RepA and Rep proteins, and their subfragments, with the maize retinoblastoma protein (ZmRb1) which is likely to be one of the interacting host proteins. We showed the self-association capability of the MSV proteins and suggest that homo- or hetero-oligomerization may play an important role in virus replication. These results provide new insights into the role of different regions of the MSV proteins in relation to transcriptional activation and regulation of viral DNA replication.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><item><term>maize streak virus</term></item><item><term>maize retinoblastoma protein</term></item><item><term>Rep proteins</term></item><item><term>transactivation</term></item><item><term>yeast two-hybrid system</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>Life Sciences</head><item><term>Biochemistry, general</term></item><item><term>Plant Sciences</term></item><item><term>Plant Pathology</term></item></list></keywords></textClass></profileDesc><revisionDesc><change 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AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Metin</GivenName><FamilyName>Bilgin</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Margaret</GivenName><FamilyName>Boulton</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Jeffrey</GivenName><GivenName>W.</GivenName><FamilyName>Davies</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff3"><AuthorName DisplayOrder="Western"><GivenName>Crisanto</GivenName><FamilyName>Gutiérrez</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Dénes</GivenName><FamilyName>Dudits</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgDivision>Institute of Plant Biology, Biological Research Center</OrgDivision><OrgName>Hungarian Academy of Sciences</OrgName><OrgAddress><Postbox>P.O. Box 521</Postbox><Postcode>6701</Postcode><City>Szeged</City><Country>Hungary</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgName>John Innes Centre</OrgName><OrgAddress><Street>Norwich Research Park</Street><City>Norwich</City><Postcode>NR4 7UH</Postcode><Country>UK</Country></OrgAddress></Affiliation><Affiliation ID="Aff3"><OrgDivision>Centro de Biología Molecular ‘Severo Ochoa’ (CSIC-UAM)</OrgDivision><OrgName>Universidad Autónoma, Cantoblanco</OrgName><OrgAddress><Postcode>28049</Postcode><City>Madrid</City><Country>Spain</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading><Para>The replication of the geminiviruses depends on the viral encoded early (complementary-sense) proteins and on host genome encoded factors. Additionally, some of the early proteins (the AL2 protein of subgroup III, and the RepA (formerly known as C1) or Rep (C1:C2) proteins of subgroup I geminiviruses) can function as transcriptional activators of virion- (V-)sense gene expression. The yeast two-hybrid system has allowed us to predict some of the functionally important regions of the maize streak virus (MSV) early proteins RepA and Rep. Defined domains of these proteins were shown to act as transactivators in yeast cells. We detected the association of the RepA and Rep proteins, and their subfragments, with the maize retinoblastoma protein (ZmRb1) which is likely to be one of the interacting host proteins. We showed the self-association capability of the MSV proteins and suggest that homo- or hetero-oligomerization may play an important role in virus replication. These results provide new insights into the role of different regions of the MSV proteins in relation to transcriptional activation and regulation of viral DNA replication.</Para></Abstract><KeywordGroup Language="En"><Keyword>maize streak virus</Keyword><Keyword>maize retinoblastoma protein</Keyword><Keyword>Rep proteins</Keyword><Keyword>transactivation</Keyword><Keyword>yeast two-hybrid system</Keyword></KeywordGroup></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Prediction of functional regions of the maize streak virus replication-associated proteins by protein-protein interaction analysis</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Prediction of functional regions of the maize streak virus replication-associated proteins by protein-protein interaction analysis</title></titleInfo><name type="personal" displayLabel="corresp"><namePart type="given">Gábor</namePart><namePart type="given">V.</namePart><namePart type="family">Horváth</namePart><affiliation>Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, 6701, Szeged, Hungary</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Aladár</namePart><namePart type="family">Pettkó-Szandtner</namePart><affiliation>Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, 6701, Szeged, Hungary</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Krisztina</namePart><namePart type="family">Nikovics</namePart><affiliation>Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, 6701, Szeged, Hungary</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Metin</namePart><namePart type="family">Bilgin</namePart><affiliation>Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, 6701, Szeged, Hungary</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Margaret</namePart><namePart type="family">Boulton</namePart><affiliation>John Innes Centre, Norwich Research Park, NR4 7UH, Norwich, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jeffrey</namePart><namePart type="given">W.</namePart><namePart type="family">Davies</namePart><affiliation>John Innes Centre, Norwich Research Park, NR4 7UH, Norwich, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Crisanto</namePart><namePart type="family">Gutiérrez</namePart><affiliation>Centro de Biología Molecular ‘Severo Ochoa’ (CSIC-UAM), Universidad Autónoma, Cantoblanco, 28049, Madrid, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Dénes</namePart><namePart type="family">Dudits</namePart><affiliation>Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, 6701, Szeged, Hungary</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Kluwer Academic Publishers</publisher><place><placeTerm type="text">Dordrecht</placeTerm></place><dateIssued encoding="w3cdtf">1998-11-01</dateIssued><copyrightDate encoding="w3cdtf">1998</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: The replication of the geminiviruses depends on the viral encoded early (complementary-sense) proteins and on host genome encoded factors. Additionally, some of the early proteins (the AL2 protein of subgroup III, and the RepA (formerly known as C1) or Rep (C1:C2) proteins of subgroup I geminiviruses) can function as transcriptional activators of virion- (V-)sense gene expression. The yeast two-hybrid system has allowed us to predict some of the functionally important regions of the maize streak virus (MSV) early proteins RepA and Rep. Defined domains of these proteins were shown to act as transactivators in yeast cells. We detected the association of the RepA and Rep proteins, and their subfragments, with the maize retinoblastoma protein (ZmRb1) which is likely to be one of the interacting host proteins. We showed the self-association capability of the MSV proteins and suggest that homo- or hetero-oligomerization may play an important role in virus replication. These results provide new insights into the role of different regions of the MSV proteins in relation to transcriptional activation and regulation of viral DNA replication.</abstract><subject lang="en"><topic>maize streak virus</topic><topic>maize retinoblastoma protein</topic><topic>Rep proteins</topic><topic>transactivation</topic><topic>yeast two-hybrid system</topic></subject><relatedItem type="host"><titleInfo><title>Plant Molecular Biology</title><subTitle>An International Journal on Molecular Biology, Molecular Genetics and Biochemistry</subTitle></titleInfo><titleInfo type="abbreviated"><title>Plant Mol Biol</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">1998-11-01</dateIssued><copyrightDate encoding="w3cdtf">1998</copyrightDate></originInfo><subject><genre>Life Sciences</genre><topic>Biochemistry, general</topic><topic>Plant Sciences</topic><topic>Plant Pathology</topic></subject><identifier type="ISSN">0167-4412</identifier><identifier type="eISSN">1573-5028</identifier><identifier type="JournalID">11103</identifier><identifier type="IssueArticleCount">30</identifier><identifier type="VolumeIssueCount">6</identifier><part><date>1998</date><detail type="volume"><number>38</number><caption>vol.</caption></detail><detail type="issue"><number>5</number><caption>no.</caption></detail><extent unit="pages"><start>699</start><end>712</end></extent></part><recordInfo><recordOrigin>Kluwer Academic Publishers, 1998</recordOrigin></recordInfo></relatedItem><identifier type="istex">8356C70381135F9971E4FAAA6B17D73AF0B99DB1</identifier><identifier type="ark">ark:/67375/VQC-FNXTQBQC-W</identifier><identifier type="DOI">10.1023/A:1006076316887</identifier><identifier type="ArticleID">169032</identifier><identifier type="ArticleID">Art4</identifier><accessCondition type="use and reproduction" contentType="copyright">Kluwer Academic Publishers, 1998</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Kluwer Academic Publishers, 1998</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-FNXTQBQC-W/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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