Expression of two cloned mRNA sequences during development and germination of spores of the sensitive fern, Onoclea sensibilis L.
Identifieur interne : 001962 ( Istex/Corpus ); précédent : 001961; suivant : 001963Expression of two cloned mRNA sequences during development and germination of spores of the sensitive fern, Onoclea sensibilis L.
Auteurs : V. Raghavan ; Joseph C. KamalaySource :
- Planta [ 0032-0935 ] ; 1993-01-01.
English descriptors
- KwdEn :
- Teeft :
- Abscisic acid, Amino, Amino acids, Angiosperm, Angiosperm embryos, Blot hybridized, Blue light, Cdna, Cdna library, Chloroplast, Clone, Differential screening, Dormant spores, Early proteins, Elip, Embryo, Fern, Fern spores, Gametophyte, Germinating spores, Germination, Hybridization, Hybridized, Kamalay, Kloppstech, Kodak film, Late embryogenesis, Mrna, Mrna sequences, Northern blot, Northern blot analysis, Northern blots, Nytran filters, Onoclea, Onoclea sensibilis, Photoinduced, Photoinduced spores, Plant physiol, Plasmid, Raghavan, Schleicher schuell, Sensibilis, Sensitive fern, Sequence identity, Sodium acetate, Sorus, Sporangium, Spore, Spore cdna clones, Spore germination, Sporogenesis.
Abstract
Abstract: A library of complementary DNA (cDNA) clones has been prepared from poly(A)+RNA of spores of the sensitive fern, Onoclea sensibilis L. By differential hybridization with labeled probes made to poly(A)+ RNA of spores, gametophytes and leaves, two spore-specific clones (pOSS68 and pOSS194) were selected and characterized. Northern blot analysis showed that RNA sequences homologous to the two cDNA clones first appear in the post-meiotic spore and increase in abundance during spore maturity. Both RNA sequences decay during photoinduced germination of the spores and do not reappear in the gametophytes. In spores imbibed in the dark under conditions which do not favor germination, no significant decrease in pOSS194-mRNA abundance is noted. In contrast, the decrease in pOSS68 mRNA in dark-imbibed spores parallels that observed in photoinduced spores. The predicted amino-acid sequence of pOSS194 has a striking similarity to the early light-inducible proteins expressed during the greening of etiolated pea and barley seedlings, whereas that of pOSS68 shows some homology to proteins encoded by late-embryogenesis-abundant mRNAs of angiosperm embryos.
Url:
DOI: 10.1007/BF00201336
Links to Exploration step
ISTEX:465FA5385D2D8E7464733263B678C4A16B418EB5Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Expression of two cloned mRNA sequences during development and germination of spores of the sensitive fern, Onoclea sensibilis L.</title>
<author><name sortKey="Raghavan, V" sort="Raghavan, V" uniqKey="Raghavan V" first="V." last="Raghavan">V. Raghavan</name>
<affiliation><mods:affiliation>Department of Plant Biology, The Ohio State University, 43210, Columbus, OH, USA</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Kamalay, Joseph C" sort="Kamalay, Joseph C" uniqKey="Kamalay J" first="Joseph C." last="Kamalay">Joseph C. Kamalay</name>
<affiliation><mods:affiliation>Department of Agronomy, The Ohio State University, 43210, Columbus, OH, USA</mods:affiliation>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:465FA5385D2D8E7464733263B678C4A16B418EB5</idno>
<date when="1993" year="1993">1993</date>
<idno type="doi">10.1007/BF00201336</idno>
<idno type="url">https://api.istex.fr/ark:/67375/1BB-TL77DVRT-Z/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001962</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001962</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Expression of two cloned mRNA sequences during development and germination of spores of the sensitive fern, Onoclea sensibilis L.</title>
<author><name sortKey="Raghavan, V" sort="Raghavan, V" uniqKey="Raghavan V" first="V." last="Raghavan">V. Raghavan</name>
<affiliation><mods:affiliation>Department of Plant Biology, The Ohio State University, 43210, Columbus, OH, USA</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Kamalay, Joseph C" sort="Kamalay, Joseph C" uniqKey="Kamalay J" first="Joseph C." last="Kamalay">Joseph C. Kamalay</name>
<affiliation><mods:affiliation>Department of Agronomy, The Ohio State University, 43210, Columbus, OH, USA</mods:affiliation>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Planta</title>
<title level="j" type="sub">An International Journal of Plant Biology</title>
<title level="j" type="abbrev">Planta</title>
<idno type="ISSN">0032-0935</idno>
<idno type="eISSN">1432-2048</idno>
<imprint><publisher>Springer-Verlag</publisher>
<pubPlace>Berlin/Heidelberg</pubPlace>
<date type="published" when="1993-01-01">1993-01-01</date>
<biblScope unit="volume">189</biblScope>
<biblScope unit="issue">1</biblScope>
<biblScope unit="page" from="1">1</biblScope>
<biblScope unit="page" to="9">9</biblScope>
</imprint>
<idno type="ISSN">0032-0935</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0032-0935</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Light and development</term>
<term>Onoclea (cDNA library)</term>
<term>Protein (early light-inducible)</term>
<term>Protein (late embryogenesis abundant)</term>
<term>Spore-specific mRNA</term>
</keywords>
<keywords scheme="Teeft" xml:lang="en"><term>Abscisic acid</term>
<term>Amino</term>
<term>Amino acids</term>
<term>Angiosperm</term>
<term>Angiosperm embryos</term>
<term>Blot hybridized</term>
<term>Blue light</term>
<term>Cdna</term>
<term>Cdna library</term>
<term>Chloroplast</term>
<term>Clone</term>
<term>Differential screening</term>
<term>Dormant spores</term>
<term>Early proteins</term>
<term>Elip</term>
<term>Embryo</term>
<term>Fern</term>
<term>Fern spores</term>
<term>Gametophyte</term>
<term>Germinating spores</term>
<term>Germination</term>
<term>Hybridization</term>
<term>Hybridized</term>
<term>Kamalay</term>
<term>Kloppstech</term>
<term>Kodak film</term>
<term>Late embryogenesis</term>
<term>Mrna</term>
<term>Mrna sequences</term>
<term>Northern blot</term>
<term>Northern blot analysis</term>
<term>Northern blots</term>
<term>Nytran filters</term>
<term>Onoclea</term>
<term>Onoclea sensibilis</term>
<term>Photoinduced</term>
<term>Photoinduced spores</term>
<term>Plant physiol</term>
<term>Plasmid</term>
<term>Raghavan</term>
<term>Schleicher schuell</term>
<term>Sensibilis</term>
<term>Sensitive fern</term>
<term>Sequence identity</term>
<term>Sodium acetate</term>
<term>Sorus</term>
<term>Sporangium</term>
<term>Spore</term>
<term>Spore cdna clones</term>
<term>Spore germination</term>
<term>Sporogenesis</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: A library of complementary DNA (cDNA) clones has been prepared from poly(A)+RNA of spores of the sensitive fern, Onoclea sensibilis L. By differential hybridization with labeled probes made to poly(A)+ RNA of spores, gametophytes and leaves, two spore-specific clones (pOSS68 and pOSS194) were selected and characterized. Northern blot analysis showed that RNA sequences homologous to the two cDNA clones first appear in the post-meiotic spore and increase in abundance during spore maturity. Both RNA sequences decay during photoinduced germination of the spores and do not reappear in the gametophytes. In spores imbibed in the dark under conditions which do not favor germination, no significant decrease in pOSS194-mRNA abundance is noted. In contrast, the decrease in pOSS68 mRNA in dark-imbibed spores parallels that observed in photoinduced spores. The predicted amino-acid sequence of pOSS194 has a striking similarity to the early light-inducible proteins expressed during the greening of etiolated pea and barley seedlings, whereas that of pOSS68 shows some homology to proteins encoded by late-embryogenesis-abundant mRNAs of angiosperm embryos.</div>
</front>
</TEI>
<istex><corpusName>springer-journals</corpusName>
<keywords><teeft><json:string>spore</json:string>
<json:string>mrna</json:string>
<json:string>germination</json:string>
<json:string>hybridized</json:string>
<json:string>cdna</json:string>
<json:string>gametophyte</json:string>
<json:string>sensibilis</json:string>
<json:string>raghavan</json:string>
<json:string>clone</json:string>
<json:string>sorus</json:string>
<json:string>hybridization</json:string>
<json:string>fern</json:string>
<json:string>amino acids</json:string>
<json:string>sporangium</json:string>
<json:string>onoclea</json:string>
<json:string>kloppstech</json:string>
<json:string>mrna sequences</json:string>
<json:string>elip</json:string>
<json:string>kamalay</json:string>
<json:string>blot hybridized</json:string>
<json:string>plasmid</json:string>
<json:string>chloroplast</json:string>
<json:string>dormant spores</json:string>
<json:string>photoinduced</json:string>
<json:string>angiosperm</json:string>
<json:string>sporogenesis</json:string>
<json:string>amino</json:string>
<json:string>onoclea sensibilis</json:string>
<json:string>spore germination</json:string>
<json:string>fern spores</json:string>
<json:string>blue light</json:string>
<json:string>northern blot analysis</json:string>
<json:string>nytran filters</json:string>
<json:string>northern blot</json:string>
<json:string>northern blots</json:string>
<json:string>early proteins</json:string>
<json:string>photoinduced spores</json:string>
<json:string>sequence identity</json:string>
<json:string>embryo</json:string>
<json:string>late embryogenesis</json:string>
<json:string>differential screening</json:string>
<json:string>schleicher schuell</json:string>
<json:string>cdna library</json:string>
<json:string>spore cdna clones</json:string>
<json:string>germinating spores</json:string>
<json:string>kodak film</json:string>
<json:string>plant physiol</json:string>
<json:string>sensitive fern</json:string>
<json:string>sodium acetate</json:string>
<json:string>abscisic acid</json:string>
<json:string>angiosperm embryos</json:string>
</teeft>
</keywords>
<author><json:item><name>V. Raghavan</name>
<affiliations><json:string>Department of Plant Biology, The Ohio State University, 43210, Columbus, OH, USA</json:string>
</affiliations>
</json:item>
<json:item><name>Joseph C. Kamalay</name>
<affiliations><json:string>Department of Agronomy, The Ohio State University, 43210, Columbus, OH, USA</json:string>
</affiliations>
</json:item>
</author>
<subject><json:item><lang><json:string>eng</json:string>
</lang>
<value>Onoclea (cDNA library)</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>Light and development</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>Protein (late embryogenesis abundant)</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>Protein (early light-inducible)</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>Spore-specific mRNA</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>bp : base pairs</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>cDNA : complementary DNA</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>ds : double-stranded</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>ELIP : early light-inducible proteins</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>LEA : late embryogenesis abundant</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>nt : nucleotide</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>ss : single stranded</value>
</json:item>
</subject>
<articleId><json:string>BF00201336</json:string>
<json:string>Art1</json:string>
</articleId>
<arkIstex>ark:/67375/1BB-TL77DVRT-Z</arkIstex>
<language><json:string>eng</json:string>
</language>
<originalGenre><json:string>OriginalPaper</json:string>
</originalGenre>
<abstract>Abstract: A library of complementary DNA (cDNA) clones has been prepared from poly(A)+RNA of spores of the sensitive fern, Onoclea sensibilis L. By differential hybridization with labeled probes made to poly(A)+ RNA of spores, gametophytes and leaves, two spore-specific clones (pOSS68 and pOSS194) were selected and characterized. Northern blot analysis showed that RNA sequences homologous to the two cDNA clones first appear in the post-meiotic spore and increase in abundance during spore maturity. Both RNA sequences decay during photoinduced germination of the spores and do not reappear in the gametophytes. In spores imbibed in the dark under conditions which do not favor germination, no significant decrease in pOSS194-mRNA abundance is noted. In contrast, the decrease in pOSS68 mRNA in dark-imbibed spores parallels that observed in photoinduced spores. The predicted amino-acid sequence of pOSS194 has a striking similarity to the early light-inducible proteins expressed during the greening of etiolated pea and barley seedlings, whereas that of pOSS68 shows some homology to proteins encoded by late-embryogenesis-abundant mRNAs of angiosperm embryos.</abstract>
<qualityIndicators><refBibsNative>false</refBibsNative>
<abstractWordCount>168</abstractWordCount>
<abstractCharCount>1166</abstractCharCount>
<keywordCount>12</keywordCount>
<score>9.016</score>
<pdfWordCount>6625</pdfWordCount>
<pdfCharCount>37592</pdfCharCount>
<pdfVersion>1.3</pdfVersion>
<pdfPageCount>9</pdfPageCount>
<pdfPageSize>595.28 x 782.928 pts</pdfPageSize>
</qualityIndicators>
<title>Expression of two cloned mRNA sequences during development and germination of spores of the sensitive fern, Onoclea sensibilis L.</title>
<pmid><json:string>7763355</json:string>
</pmid>
<genre><json:string>research-article</json:string>
</genre>
<host><title>Planta</title>
<language><json:string>unknown</json:string>
</language>
<publicationDate>1993</publicationDate>
<copyrightDate>1993</copyrightDate>
<issn><json:string>0032-0935</json:string>
</issn>
<eissn><json:string>1432-2048</json:string>
</eissn>
<journalId><json:string>425</json:string>
</journalId>
<volume>189</volume>
<issue>1</issue>
<pages><first>1</first>
<last>9</last>
</pages>
<genre><json:string>journal</json:string>
</genre>
<subject><json:item><value>Agriculture</value>
</json:item>
<json:item><value>Ecology</value>
</json:item>
<json:item><value>Forestry</value>
</json:item>
<json:item><value>Plant Sciences</value>
</json:item>
</subject>
</host>
<namedEntities><unitex><date></date>
<geogName></geogName>
<orgName></orgName>
<orgName_funder></orgName_funder>
<orgName_provider></orgName_provider>
<persName></persName>
<placeName></placeName>
<ref_url></ref_url>
<ref_bibl></ref_bibl>
<bibl></bibl>
</unitex>
</namedEntities>
<ark><json:string>ark:/67375/1BB-TL77DVRT-Z</json:string>
</ark>
<categories><wos><json:string>1 - science</json:string>
<json:string>2 - plant sciences</json:string>
</wos>
<scienceMetrix><json:string>1 - natural sciences</json:string>
<json:string>2 - biology</json:string>
<json:string>3 - plant biology & botany</json:string>
</scienceMetrix>
<scopus><json:string>1 - Life Sciences</json:string>
<json:string>2 - Agricultural and Biological Sciences</json:string>
<json:string>3 - Plant Science</json:string>
<json:string>1 - Life Sciences</json:string>
<json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string>
<json:string>3 - Genetics</json:string>
</scopus>
<inist><json:string>1 - sciences appliquees, technologies et medecines</json:string>
<json:string>2 - sciences biologiques et medicales</json:string>
<json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string>
<json:string>4 - psychologie. psychophysiologie</json:string>
</inist>
</categories>
<publicationDate>1993</publicationDate>
<copyrightDate>1993</copyrightDate>
<doi><json:string>10.1007/BF00201336</json:string>
</doi>
<id>465FA5385D2D8E7464733263B678C4A16B418EB5</id>
<score>1</score>
<fulltext><json:item><extension>pdf</extension>
<original>true</original>
<mimetype>application/pdf</mimetype>
<uri>https://api.istex.fr/ark:/67375/1BB-TL77DVRT-Z/fulltext.pdf</uri>
</json:item>
<json:item><extension>zip</extension>
<original>false</original>
<mimetype>application/zip</mimetype>
<uri>https://api.istex.fr/ark:/67375/1BB-TL77DVRT-Z/bundle.zip</uri>
</json:item>
<istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/1BB-TL77DVRT-Z/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Expression of two cloned mRNA sequences during development and germination of spores of the sensitive fern, Onoclea sensibilis L.</title>
</titleStmt>
<publicationStmt><authority>ISTEX</authority>
<publisher scheme="https://scientific-publisher.data.istex.fr">Springer-Verlag</publisher>
<pubPlace>Berlin/Heidelberg</pubPlace>
<availability><licence><p>Springer-Verlag, 1993</p>
</licence>
<p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</p>
</availability>
<date>1992-06-17</date>
</publicationStmt>
<notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note>
<note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note>
</notesStmt>
<sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Expression of two cloned mRNA sequences during development and germination of spores of the sensitive fern, Onoclea sensibilis L.</title>
<author xml:id="author-0000"><persName><forename type="first">V.</forename>
<surname>Raghavan</surname>
</persName>
<affiliation>Department of Plant Biology, The Ohio State University, 43210, Columbus, OH, USA</affiliation>
</author>
<author xml:id="author-0001" corresp="yes"><persName><forename type="first">Joseph</forename>
<surname>Kamalay</surname>
</persName>
<affiliation>Department of Agronomy, The Ohio State University, 43210, Columbus, OH, USA</affiliation>
</author>
<idno type="istex">465FA5385D2D8E7464733263B678C4A16B418EB5</idno>
<idno type="ark">ark:/67375/1BB-TL77DVRT-Z</idno>
<idno type="DOI">10.1007/BF00201336</idno>
<idno type="article-id">BF00201336</idno>
<idno type="article-id">Art1</idno>
</analytic>
<monogr><title level="j">Planta</title>
<title level="j" type="sub">An International Journal of Plant Biology</title>
<title level="j" type="abbrev">Planta</title>
<idno type="pISSN">0032-0935</idno>
<idno type="eISSN">1432-2048</idno>
<idno type="journal-ID">true</idno>
<idno type="issue-article-count">22</idno>
<idno type="volume-issue-count">4</idno>
<imprint><publisher>Springer-Verlag</publisher>
<pubPlace>Berlin/Heidelberg</pubPlace>
<date type="published" when="1993-01-01"></date>
<biblScope unit="volume">189</biblScope>
<biblScope unit="issue">1</biblScope>
<biblScope unit="page" from="1">1</biblScope>
<biblScope unit="page" to="9">9</biblScope>
</imprint>
</monogr>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><creation><date>1992-06-17</date>
</creation>
<langUsage><language ident="en">en</language>
</langUsage>
<abstract xml:lang="en"><p>Abstract: A library of complementary DNA (cDNA) clones has been prepared from poly(A)+RNA of spores of the sensitive fern, Onoclea sensibilis L. By differential hybridization with labeled probes made to poly(A)+ RNA of spores, gametophytes and leaves, two spore-specific clones (pOSS68 and pOSS194) were selected and characterized. Northern blot analysis showed that RNA sequences homologous to the two cDNA clones first appear in the post-meiotic spore and increase in abundance during spore maturity. Both RNA sequences decay during photoinduced germination of the spores and do not reappear in the gametophytes. In spores imbibed in the dark under conditions which do not favor germination, no significant decrease in pOSS194-mRNA abundance is noted. In contrast, the decrease in pOSS68 mRNA in dark-imbibed spores parallels that observed in photoinduced spores. The predicted amino-acid sequence of pOSS194 has a striking similarity to the early light-inducible proteins expressed during the greening of etiolated pea and barley seedlings, whereas that of pOSS68 shows some homology to proteins encoded by late-embryogenesis-abundant mRNAs of angiosperm embryos.</p>
</abstract>
<textClass xml:lang="en"><keywords scheme="keyword"><list><head>Key words</head>
<item><term>Onoclea (cDNA library)</term>
</item>
<item><term>Light and development</term>
</item>
<item><term>Protein (late embryogenesis abundant)</term>
</item>
<item><term>Protein (early light-inducible)</term>
</item>
<item><term>Spore-specific mRNA</term>
</item>
</list>
</keywords>
</textClass>
<textClass><keywords scheme="keyword"><list><head>Abbreviations</head>
<item><term>bp</term>
<term>base pairs</term>
</item>
<item><term>cDNA</term>
<term>complementary DNA</term>
</item>
<item><term>ds</term>
<term>double-stranded</term>
</item>
<item><term>ELIP</term>
<term>early light-inducible proteins</term>
</item>
<item><term>LEA</term>
<term>late embryogenesis abundant</term>
</item>
<item><term>nt</term>
<term>nucleotide</term>
</item>
<item><term>ss</term>
<term>single stranded</term>
</item>
</list>
</keywords>
</textClass>
<textClass><keywords scheme="Journal Subject"><list><head>Life Sciences</head>
<item><term>Agriculture</term>
</item>
<item><term>Ecology</term>
</item>
<item><term>Forestry</term>
</item>
<item><term>Plant Sciences</term>
</item>
</list>
</keywords>
</textClass>
</profileDesc>
<revisionDesc><change when="1992-06-17">Created</change>
<change when="1993-01-01">Published</change>
</revisionDesc>
</teiHeader>
</istex:fulltextTEI>
<json:item><extension>txt</extension>
<original>false</original>
<mimetype>text/plain</mimetype>
<uri>https://api.istex.fr/ark:/67375/1BB-TL77DVRT-Z/fulltext.txt</uri>
</json:item>
</fulltext>
<metadata><istex:metadataXml wicri:clean="corpus springer-journals not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration>
<istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" name="istex:docType"></istex:docType>
<istex:document><Publisher><PublisherInfo><PublisherName>Springer-Verlag</PublisherName>
<PublisherLocation>Berlin/Heidelberg</PublisherLocation>
</PublisherInfo>
<Journal><JournalInfo JournalProductType="ArchiveJournal" NumberingStyle="Unnumbered"><JournalID>425</JournalID>
<JournalPrintISSN>0032-0935</JournalPrintISSN>
<JournalElectronicISSN>1432-2048</JournalElectronicISSN>
<JournalTitle>Planta</JournalTitle>
<JournalSubTitle>An International Journal of Plant Biology</JournalSubTitle>
<JournalAbbreviatedTitle>Planta</JournalAbbreviatedTitle>
<JournalSubjectGroup><JournalSubject Type="Primary">Life Sciences</JournalSubject>
<JournalSubject Type="Secondary">Agriculture</JournalSubject>
<JournalSubject Type="Secondary">Ecology</JournalSubject>
<JournalSubject Type="Secondary">Forestry</JournalSubject>
<JournalSubject Type="Secondary">Plant Sciences</JournalSubject>
</JournalSubjectGroup>
</JournalInfo>
<Volume><VolumeInfo VolumeType="Regular" TocLevels="0"><VolumeIDStart>189</VolumeIDStart>
<VolumeIDEnd>189</VolumeIDEnd>
<VolumeIssueCount>4</VolumeIssueCount>
</VolumeInfo>
<Issue IssueType="Regular"><IssueInfo TocLevels="0"><IssueIDStart>1</IssueIDStart>
<IssueIDEnd>1</IssueIDEnd>
<IssueArticleCount>22</IssueArticleCount>
<IssueHistory><CoverDate><Year>1993</Year>
<Month>1</Month>
</CoverDate>
</IssueHistory>
<IssueCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName>
<CopyrightYear>1993</CopyrightYear>
</IssueCopyright>
</IssueInfo>
<Article ID="Art1"><ArticleInfo Language="En" ArticleType="OriginalPaper" NumberingStyle="Unnumbered" TocLevels="0" ContainsESM="No"><ArticleID>BF00201336</ArticleID>
<ArticleDOI>10.1007/BF00201336</ArticleDOI>
<ArticleSequenceNumber>1</ArticleSequenceNumber>
<ArticleTitle Language="En">Expression of two cloned mRNA sequences during development and germination of spores of the sensitive fern, <Emphasis Type="Italic">Onoclea sensibilis</Emphasis>
L.</ArticleTitle>
<ArticleFirstPage>1</ArticleFirstPage>
<ArticleLastPage>9</ArticleLastPage>
<ArticleHistory><RegistrationDate><Year>2004</Year>
<Month>7</Month>
<Day>15</Day>
</RegistrationDate>
<Received><Year>1992</Year>
<Month>6</Month>
<Day>17</Day>
</Received>
<Accepted><Year>1992</Year>
<Month>8</Month>
<Day>8</Day>
</Accepted>
</ArticleHistory>
<ArticleCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName>
<CopyrightYear>1993</CopyrightYear>
</ArticleCopyright>
<ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant>
<AbstractGrant Grant="OpenAccess"></AbstractGrant>
<BodyPDFGrant Grant="Restricted"></BodyPDFGrant>
<BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant>
<BibliographyGrant Grant="Restricted"></BibliographyGrant>
<ESMGrant Grant="Restricted"></ESMGrant>
</ArticleGrants>
<ArticleContext><JournalID>425</JournalID>
<VolumeIDStart>189</VolumeIDStart>
<VolumeIDEnd>189</VolumeIDEnd>
<IssueIDStart>1</IssueIDStart>
<IssueIDEnd>1</IssueIDEnd>
</ArticleContext>
</ArticleInfo>
<ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>V.</GivenName>
<FamilyName>Raghavan</FamilyName>
</AuthorName>
<Contact><Fax>1 (614) 292 6345</Fax>
</Contact>
</Author>
<Author AffiliationIDS="Aff2" CorrespondingAffiliationID="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Joseph</GivenName>
<GivenName>C.</GivenName>
<FamilyName>Kamalay</FamilyName>
</AuthorName>
</Author>
<Affiliation ID="Aff1"><OrgDivision>Department of Plant Biology</OrgDivision>
<OrgName>The Ohio State University</OrgName>
<OrgAddress><Postcode>43210</Postcode>
<City>Columbus</City>
<State>OH</State>
<Country>USA</Country>
</OrgAddress>
</Affiliation>
<Affiliation ID="Aff2"><OrgDivision>Department of Agronomy</OrgDivision>
<OrgName>The Ohio State University</OrgName>
<OrgAddress><Postcode>43210</Postcode>
<City>Columbus</City>
<State>OH</State>
<Country>USA</Country>
</OrgAddress>
</Affiliation>
</AuthorGroup>
<Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading>
<Para>A library of complementary DNA (cDNA) clones has been prepared from poly(A)<Superscript>+</Superscript>
RNA of spores of the sensitive fern, <Emphasis Type="Italic">Onoclea sensibilis</Emphasis>
L. By differential hybridization with labeled probes made to poly(A)<Superscript>+</Superscript>
RNA of spores, gametophytes and leaves, two spore-specific clones (pOSS68 and pOSS194) were selected and characterized. Northern blot analysis showed that RNA sequences homologous to the two cDNA clones first appear in the post-meiotic spore and increase in abundance during spore maturity. Both RNA sequences decay during photoinduced germination of the spores and do not reappear in the gametophytes. In spores imbibed in the dark under conditions which do not favor germination, no significant decrease in pOSS194-mRNA abundance is noted. In contrast, the decrease in pOSS68 mRNA in dark-imbibed spores parallels that observed in photoinduced spores. The predicted amino-acid sequence of pOSS194 has a striking similarity to the early light-inducible proteins expressed during the greening of etiolated pea and barley seedlings, whereas that of pOSS68 shows some homology to proteins encoded by late-embryogenesis-abundant mRNAs of angiosperm embryos.</Para>
</Abstract>
<KeywordGroup Language="En"><Heading>Key words</Heading>
<Keyword><Emphasis Type="Italic">Onoclea</Emphasis>
(cDNA library)</Keyword>
<Keyword>Light and development</Keyword>
<Keyword>Protein (late embryogenesis abundant)</Keyword>
<Keyword>Protein (early light-inducible)</Keyword>
<Keyword>Spore-specific mRNA</Keyword>
</KeywordGroup>
<AbbreviationGroup><Heading>Abbreviations</Heading>
<DefinitionList><DefinitionListEntry><Term>bp</Term>
<Description><Para>base pairs</Para>
</Description>
</DefinitionListEntry>
<DefinitionListEntry><Term>cDNA</Term>
<Description><Para>complementary DNA</Para>
</Description>
</DefinitionListEntry>
<DefinitionListEntry><Term>ds</Term>
<Description><Para>double-stranded</Para>
</Description>
</DefinitionListEntry>
<DefinitionListEntry><Term>ELIP</Term>
<Description><Para>early light-inducible proteins</Para>
</Description>
</DefinitionListEntry>
<DefinitionListEntry><Term>LEA</Term>
<Description><Para>late embryogenesis abundant</Para>
</Description>
</DefinitionListEntry>
<DefinitionListEntry><Term>nt</Term>
<Description><Para>nucleotide</Para>
</Description>
</DefinitionListEntry>
<DefinitionListEntry><Term>ss</Term>
<Description><Para>single stranded</Para>
</Description>
</DefinitionListEntry>
</DefinitionList>
</AbbreviationGroup>
<ArticleNote Type="Misc"><SimplePara>This work was partially supported by a NASA grant (NAGW-901) and by an allocation from the Research Challenge Investigators' Fund of the Ohio State University to V.R. Thanks are due to Mr. Clayton L. Rugh for sequencing our clones and to Dr. Paul A. Fuerst for help in the computer search of sequence alignments.</SimplePara>
</ArticleNote>
</ArticleHeader>
<NoBody></NoBody>
</Article>
</Issue>
</Volume>
</Journal>
</Publisher>
</istex:document>
</istex:metadataXml>
<mods version="3.6"><titleInfo lang="en"><title>Expression of two cloned mRNA sequences during development and germination of spores of the sensitive fern, Onoclea sensibilis L.</title>
</titleInfo>
<titleInfo type="alternative" contentType="CDATA"><title>Expression of two cloned mRNA sequences during development and germination of spores of the sensitive fern, Onoclea sensibilis L.</title>
</titleInfo>
<name type="personal"><namePart type="given">V.</namePart>
<namePart type="family">Raghavan</namePart>
<affiliation>Department of Plant Biology, The Ohio State University, 43210, Columbus, OH, USA</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal" displayLabel="corresp"><namePart type="given">Joseph</namePart>
<namePart type="given">C.</namePart>
<namePart type="family">Kamalay</namePart>
<affiliation>Department of Agronomy, The Ohio State University, 43210, Columbus, OH, USA</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<typeOfResource>text</typeOfResource>
<genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre>
<originInfo><publisher>Springer-Verlag</publisher>
<place><placeTerm type="text">Berlin/Heidelberg</placeTerm>
</place>
<dateCreated encoding="w3cdtf">1992-06-17</dateCreated>
<dateIssued encoding="w3cdtf">1993-01-01</dateIssued>
<copyrightDate encoding="w3cdtf">1993</copyrightDate>
</originInfo>
<language><languageTerm type="code" authority="rfc3066">en</languageTerm>
<languageTerm type="code" authority="iso639-2b">eng</languageTerm>
</language>
<abstract lang="en">Abstract: A library of complementary DNA (cDNA) clones has been prepared from poly(A)+RNA of spores of the sensitive fern, Onoclea sensibilis L. By differential hybridization with labeled probes made to poly(A)+ RNA of spores, gametophytes and leaves, two spore-specific clones (pOSS68 and pOSS194) were selected and characterized. Northern blot analysis showed that RNA sequences homologous to the two cDNA clones first appear in the post-meiotic spore and increase in abundance during spore maturity. Both RNA sequences decay during photoinduced germination of the spores and do not reappear in the gametophytes. In spores imbibed in the dark under conditions which do not favor germination, no significant decrease in pOSS194-mRNA abundance is noted. In contrast, the decrease in pOSS68 mRNA in dark-imbibed spores parallels that observed in photoinduced spores. The predicted amino-acid sequence of pOSS194 has a striking similarity to the early light-inducible proteins expressed during the greening of etiolated pea and barley seedlings, whereas that of pOSS68 shows some homology to proteins encoded by late-embryogenesis-abundant mRNAs of angiosperm embryos.</abstract>
<subject lang="en"><genre>Key words</genre>
<topic>Onoclea (cDNA library)</topic>
<topic>Light and development</topic>
<topic>Protein (late embryogenesis abundant)</topic>
<topic>Protein (early light-inducible)</topic>
<topic>Spore-specific mRNA</topic>
</subject>
<subject><genre>Abbreviations</genre>
<topic>bp : base pairs</topic>
<topic>cDNA : complementary DNA</topic>
<topic>ds : double-stranded</topic>
<topic>ELIP : early light-inducible proteins</topic>
<topic>LEA : late embryogenesis abundant</topic>
<topic>nt : nucleotide</topic>
<topic>ss : single stranded</topic>
</subject>
<relatedItem type="host"><titleInfo><title>Planta</title>
<subTitle>An International Journal of Plant Biology</subTitle>
</titleInfo>
<titleInfo type="abbreviated"><title>Planta</title>
</titleInfo>
<genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre>
<originInfo><publisher>Springer</publisher>
<dateIssued encoding="w3cdtf">1993-01-01</dateIssued>
<copyrightDate encoding="w3cdtf">1993</copyrightDate>
</originInfo>
<subject><genre>Life Sciences</genre>
<topic>Agriculture</topic>
<topic>Ecology</topic>
<topic>Forestry</topic>
<topic>Plant Sciences</topic>
</subject>
<identifier type="ISSN">0032-0935</identifier>
<identifier type="eISSN">1432-2048</identifier>
<identifier type="JournalID">425</identifier>
<identifier type="IssueArticleCount">22</identifier>
<identifier type="VolumeIssueCount">4</identifier>
<part><date>1993</date>
<detail type="volume"><number>189</number>
<caption>vol.</caption>
</detail>
<detail type="issue"><number>1</number>
<caption>no.</caption>
</detail>
<extent unit="pages"><start>1</start>
<end>9</end>
</extent>
</part>
<recordInfo><recordOrigin>Springer-Verlag, 1993</recordOrigin>
</recordInfo>
</relatedItem>
<identifier type="istex">465FA5385D2D8E7464733263B678C4A16B418EB5</identifier>
<identifier type="ark">ark:/67375/1BB-TL77DVRT-Z</identifier>
<identifier type="DOI">10.1007/BF00201336</identifier>
<identifier type="ArticleID">BF00201336</identifier>
<identifier type="ArticleID">Art1</identifier>
<accessCondition type="use and reproduction" contentType="copyright">Springer-Verlag, 1993</accessCondition>
<recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource>
<recordOrigin>Springer-Verlag, 1993</recordOrigin>
</recordInfo>
</mods>
<json:item><extension>json</extension>
<original>false</original>
<mimetype>application/json</mimetype>
<uri>https://api.istex.fr/ark:/67375/1BB-TL77DVRT-Z/record.json</uri>
</json:item>
</metadata>
<serie></serie>
</istex>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001962 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 001962 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Istex |étape= Corpus |type= RBID |clé= ISTEX:465FA5385D2D8E7464733263B678C4A16B418EB5 |texte= Expression of two cloned mRNA sequences during development and germination of spores of the sensitive fern, Onoclea sensibilis L. }}
![]() | This area was generated with Dilib version V0.6.33. | ![]() |