Expression of two cloned mRNA sequences during development and germination of spores of the sensitive fern, Onoclea sensibilis L.
Identifieur interne : 001962 ( Istex/Corpus ); précédent : 001961; suivant : 001963Expression of two cloned mRNA sequences during development and germination of spores of the sensitive fern, Onoclea sensibilis L.
Auteurs : V. Raghavan ; Joseph C. KamalaySource :
- Planta [ 0032-0935 ] ; 1993-01-01.
English descriptors
- KwdEn :
- Teeft :
- Abscisic acid, Amino, Amino acids, Angiosperm, Angiosperm embryos, Blot hybridized, Blue light, Cdna, Cdna library, Chloroplast, Clone, Differential screening, Dormant spores, Early proteins, Elip, Embryo, Fern, Fern spores, Gametophyte, Germinating spores, Germination, Hybridization, Hybridized, Kamalay, Kloppstech, Kodak film, Late embryogenesis, Mrna, Mrna sequences, Northern blot, Northern blot analysis, Northern blots, Nytran filters, Onoclea, Onoclea sensibilis, Photoinduced, Photoinduced spores, Plant physiol, Plasmid, Raghavan, Schleicher schuell, Sensibilis, Sensitive fern, Sequence identity, Sodium acetate, Sorus, Sporangium, Spore, Spore cdna clones, Spore germination, Sporogenesis.
Abstract
Abstract: A library of complementary DNA (cDNA) clones has been prepared from poly(A)+RNA of spores of the sensitive fern, Onoclea sensibilis L. By differential hybridization with labeled probes made to poly(A)+ RNA of spores, gametophytes and leaves, two spore-specific clones (pOSS68 and pOSS194) were selected and characterized. Northern blot analysis showed that RNA sequences homologous to the two cDNA clones first appear in the post-meiotic spore and increase in abundance during spore maturity. Both RNA sequences decay during photoinduced germination of the spores and do not reappear in the gametophytes. In spores imbibed in the dark under conditions which do not favor germination, no significant decrease in pOSS194-mRNA abundance is noted. In contrast, the decrease in pOSS68 mRNA in dark-imbibed spores parallels that observed in photoinduced spores. The predicted amino-acid sequence of pOSS194 has a striking similarity to the early light-inducible proteins expressed during the greening of etiolated pea and barley seedlings, whereas that of pOSS68 shows some homology to proteins encoded by late-embryogenesis-abundant mRNAs of angiosperm embryos.
Url:
DOI: 10.1007/BF00201336
Links to Exploration step
ISTEX:465FA5385D2D8E7464733263B678C4A16B418EB5Le document en format XML
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By differential hybridization with labeled probes made to poly(A)+ RNA of spores, gametophytes and leaves, two spore-specific clones (pOSS68 and pOSS194) were selected and characterized. Northern blot analysis showed that RNA sequences homologous to the two cDNA clones first appear in the post-meiotic spore and increase in abundance during spore maturity. Both RNA sequences decay during photoinduced germination of the spores and do not reappear in the gametophytes. In spores imbibed in the dark under conditions which do not favor germination, no significant decrease in pOSS194-mRNA abundance is noted. In contrast, the decrease in pOSS68 mRNA in dark-imbibed spores parallels that observed in photoinduced spores. The predicted amino-acid sequence of pOSS194 has a striking similarity to the early light-inducible proteins expressed during the greening of etiolated pea and barley seedlings, whereas that of pOSS68 shows some homology to proteins encoded by late-embryogenesis-abundant mRNAs of angiosperm embryos.</div></front></TEI><istex><corpusName>springer-journals</corpusName><keywords><teeft><json:string>spore</json:string><json:string>mrna</json:string><json:string>germination</json:string><json:string>hybridized</json:string><json:string>cdna</json:string><json:string>gametophyte</json:string><json:string>sensibilis</json:string><json:string>raghavan</json:string><json:string>clone</json:string><json:string>sorus</json:string><json:string>hybridization</json:string><json:string>fern</json:string><json:string>amino acids</json:string><json:string>sporangium</json:string><json:string>onoclea</json:string><json:string>kloppstech</json:string><json:string>mrna sequences</json:string><json:string>elip</json:string><json:string>kamalay</json:string><json:string>blot hybridized</json:string><json:string>plasmid</json:string><json:string>chloroplast</json:string><json:string>dormant spores</json:string><json:string>photoinduced</json:string><json:string>angiosperm</json:string><json:string>sporogenesis</json:string><json:string>amino</json:string><json:string>onoclea sensibilis</json:string><json:string>spore germination</json:string><json:string>fern spores</json:string><json:string>blue light</json:string><json:string>northern blot analysis</json:string><json:string>nytran filters</json:string><json:string>northern blot</json:string><json:string>northern blots</json:string><json:string>early proteins</json:string><json:string>photoinduced spores</json:string><json:string>sequence identity</json:string><json:string>embryo</json:string><json:string>late embryogenesis</json:string><json:string>differential screening</json:string><json:string>schleicher schuell</json:string><json:string>cdna library</json:string><json:string>spore cdna clones</json:string><json:string>germinating spores</json:string><json:string>kodak film</json:string><json:string>plant physiol</json:string><json:string>sensitive fern</json:string><json:string>sodium acetate</json:string><json:string>abscisic acid</json:string><json:string>angiosperm embryos</json:string></teeft></keywords><author><json:item><name>V. 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By differential hybridization with labeled probes made to poly(A)+ RNA of spores, gametophytes and leaves, two spore-specific clones (pOSS68 and pOSS194) were selected and characterized. Northern blot analysis showed that RNA sequences homologous to the two cDNA clones first appear in the post-meiotic spore and increase in abundance during spore maturity. Both RNA sequences decay during photoinduced germination of the spores and do not reappear in the gametophytes. In spores imbibed in the dark under conditions which do not favor germination, no significant decrease in pOSS194-mRNA abundance is noted. In contrast, the decrease in pOSS68 mRNA in dark-imbibed spores parallels that observed in photoinduced spores. The predicted amino-acid sequence of pOSS194 has a striking similarity to the early light-inducible proteins expressed during the greening of etiolated pea and barley seedlings, whereas that of pOSS68 shows some homology to proteins encoded by late-embryogenesis-abundant mRNAs of angiosperm embryos.</abstract><qualityIndicators><refBibsNative>false</refBibsNative><abstractWordCount>168</abstractWordCount><abstractCharCount>1166</abstractCharCount><keywordCount>12</keywordCount><score>9.016</score><pdfWordCount>6625</pdfWordCount><pdfCharCount>37592</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>9</pdfPageCount><pdfPageSize>595.28 x 782.928 pts</pdfPageSize></qualityIndicators><title>Expression of two cloned mRNA sequences during development and germination of spores of the sensitive fern, Onoclea sensibilis L.</title><pmid><json:string>7763355</json:string></pmid><genre><json:string>research-article</json:string></genre><host><title>Planta</title><language><json:string>unknown</json:string></language><publicationDate>1993</publicationDate><copyrightDate>1993</copyrightDate><issn><json:string>0032-0935</json:string></issn><eissn><json:string>1432-2048</json:string></eissn><journalId><json:string>425</json:string></journalId><volume>189</volume><issue>1</issue><pages><first>1</first><last>9</last></pages><genre><json:string>journal</json:string></genre><subject><json:item><value>Agriculture</value></json:item><json:item><value>Ecology</value></json:item><json:item><value>Forestry</value></json:item><json:item><value>Plant Sciences</value></json:item></subject></host><namedEntities><unitex><date></date><geogName></geogName><orgName></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName></persName><placeName></placeName><ref_url></ref_url><ref_bibl></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/1BB-TL77DVRT-Z</json:string></ark><categories><wos><json:string>1 - 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By differential hybridization with labeled probes made to poly(A)+ RNA of spores, gametophytes and leaves, two spore-specific clones (pOSS68 and pOSS194) were selected and characterized. Northern blot analysis showed that RNA sequences homologous to the two cDNA clones first appear in the post-meiotic spore and increase in abundance during spore maturity. Both RNA sequences decay during photoinduced germination of the spores and do not reappear in the gametophytes. In spores imbibed in the dark under conditions which do not favor germination, no significant decrease in pOSS194-mRNA abundance is noted. In contrast, the decrease in pOSS68 mRNA in dark-imbibed spores parallels that observed in photoinduced spores. The predicted amino-acid sequence of pOSS194 has a striking similarity to the early light-inducible proteins expressed during the greening of etiolated pea and barley seedlings, whereas that of pOSS68 shows some homology to proteins encoded by late-embryogenesis-abundant mRNAs of angiosperm embryos.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Key words</head><item><term>Onoclea (cDNA library)</term></item><item><term>Light and development</term></item><item><term>Protein (late embryogenesis abundant)</term></item><item><term>Protein (early light-inducible)</term></item><item><term>Spore-specific mRNA</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list><head>Abbreviations</head><item><term>bp</term><term>base pairs</term></item><item><term>cDNA</term><term>complementary DNA</term></item><item><term>ds</term><term>double-stranded</term></item><item><term>ELIP</term><term>early light-inducible proteins</term></item><item><term>LEA</term><term>late embryogenesis abundant</term></item><item><term>nt</term><term>nucleotide</term></item><item><term>ss</term><term>single stranded</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>Life Sciences</head><item><term>Agriculture</term></item><item><term>Ecology</term></item><item><term>Forestry</term></item><item><term>Plant Sciences</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1992-06-17">Created</change><change when="1993-01-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/1BB-TL77DVRT-Z/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus springer-journals not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" 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Type="Secondary">Forestry</JournalSubject><JournalSubject Type="Secondary">Plant Sciences</JournalSubject></JournalSubjectGroup></JournalInfo><Volume><VolumeInfo VolumeType="Regular" TocLevels="0"><VolumeIDStart>189</VolumeIDStart><VolumeIDEnd>189</VolumeIDEnd><VolumeIssueCount>4</VolumeIssueCount></VolumeInfo><Issue IssueType="Regular"><IssueInfo TocLevels="0"><IssueIDStart>1</IssueIDStart><IssueIDEnd>1</IssueIDEnd><IssueArticleCount>22</IssueArticleCount><IssueHistory><CoverDate><Year>1993</Year><Month>1</Month></CoverDate></IssueHistory><IssueCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>1993</CopyrightYear></IssueCopyright></IssueInfo><Article ID="Art1"><ArticleInfo Language="En" ArticleType="OriginalPaper" NumberingStyle="Unnumbered" TocLevels="0" ContainsESM="No"><ArticleID>BF00201336</ArticleID><ArticleDOI>10.1007/BF00201336</ArticleDOI><ArticleSequenceNumber>1</ArticleSequenceNumber><ArticleTitle Language="En">Expression of two cloned mRNA sequences during development and germination of spores of the sensitive fern, <Emphasis Type="Italic">Onoclea sensibilis</Emphasis> L.</ArticleTitle><ArticleFirstPage>1</ArticleFirstPage><ArticleLastPage>9</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2004</Year><Month>7</Month><Day>15</Day></RegistrationDate><Received><Year>1992</Year><Month>6</Month><Day>17</Day></Received><Accepted><Year>1992</Year><Month>8</Month><Day>8</Day></Accepted></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>1993</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants><ArticleContext><JournalID>425</JournalID><VolumeIDStart>189</VolumeIDStart><VolumeIDEnd>189</VolumeIDEnd><IssueIDStart>1</IssueIDStart><IssueIDEnd>1</IssueIDEnd></ArticleContext></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>V.</GivenName><FamilyName>Raghavan</FamilyName></AuthorName><Contact><Fax>1 (614) 292 6345</Fax></Contact></Author><Author AffiliationIDS="Aff2" CorrespondingAffiliationID="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Joseph</GivenName><GivenName>C.</GivenName><FamilyName>Kamalay</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgDivision>Department of Plant Biology</OrgDivision><OrgName>The Ohio State University</OrgName><OrgAddress><Postcode>43210</Postcode><City>Columbus</City><State>OH</State><Country>USA</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>Department of Agronomy</OrgDivision><OrgName>The Ohio State University</OrgName><OrgAddress><Postcode>43210</Postcode><City>Columbus</City><State>OH</State><Country>USA</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading><Para>A library of complementary DNA (cDNA) clones has been prepared from poly(A)<Superscript>+</Superscript>RNA of spores of the sensitive fern, <Emphasis Type="Italic">Onoclea sensibilis</Emphasis> L. By differential hybridization with labeled probes made to poly(A)<Superscript>+</Superscript> RNA of spores, gametophytes and leaves, two spore-specific clones (pOSS68 and pOSS194) were selected and characterized. Northern blot analysis showed that RNA sequences homologous to the two cDNA clones first appear in the post-meiotic spore and increase in abundance during spore maturity. Both RNA sequences decay during photoinduced germination of the spores and do not reappear in the gametophytes. In spores imbibed in the dark under conditions which do not favor germination, no significant decrease in pOSS194-mRNA abundance is noted. In contrast, the decrease in pOSS68 mRNA in dark-imbibed spores parallels that observed in photoinduced spores. The predicted amino-acid sequence of pOSS194 has a striking similarity to the early light-inducible proteins expressed during the greening of etiolated pea and barley seedlings, whereas that of pOSS68 shows some homology to proteins encoded by late-embryogenesis-abundant mRNAs of angiosperm embryos.</Para></Abstract><KeywordGroup Language="En"><Heading>Key words</Heading><Keyword><Emphasis Type="Italic">Onoclea</Emphasis> (cDNA library)</Keyword><Keyword>Light and development</Keyword><Keyword>Protein (late embryogenesis abundant)</Keyword><Keyword>Protein (early light-inducible)</Keyword><Keyword>Spore-specific mRNA</Keyword></KeywordGroup><AbbreviationGroup><Heading>Abbreviations</Heading><DefinitionList><DefinitionListEntry><Term>bp</Term><Description><Para>base pairs</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>cDNA</Term><Description><Para>complementary DNA</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>ds</Term><Description><Para>double-stranded</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>ELIP</Term><Description><Para>early light-inducible proteins</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>LEA</Term><Description><Para>late embryogenesis abundant</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>nt</Term><Description><Para>nucleotide</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>ss</Term><Description><Para>single stranded</Para></Description></DefinitionListEntry></DefinitionList></AbbreviationGroup><ArticleNote Type="Misc"><SimplePara>This work was partially supported by a NASA grant (NAGW-901) and by an allocation from the Research Challenge Investigators' Fund of the Ohio State University to V.R. Thanks are due to Mr. Clayton L. Rugh for sequencing our clones and to Dr. Paul A. Fuerst for help in the computer search of sequence alignments.</SimplePara></ArticleNote></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Expression of two cloned mRNA sequences during development and germination of spores of the sensitive fern, Onoclea sensibilis L.</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Expression of two cloned mRNA sequences during development and germination of spores of the sensitive fern, Onoclea sensibilis L.</title></titleInfo><name type="personal"><namePart type="given">V.</namePart><namePart type="family">Raghavan</namePart><affiliation>Department of Plant Biology, The Ohio State University, 43210, Columbus, OH, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Joseph</namePart><namePart type="given">C.</namePart><namePart type="family">Kamalay</namePart><affiliation>Department of Agronomy, The Ohio State University, 43210, Columbus, OH, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer-Verlag</publisher><place><placeTerm type="text">Berlin/Heidelberg</placeTerm></place><dateCreated encoding="w3cdtf">1992-06-17</dateCreated><dateIssued encoding="w3cdtf">1993-01-01</dateIssued><copyrightDate encoding="w3cdtf">1993</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: A library of complementary DNA (cDNA) clones has been prepared from poly(A)+RNA of spores of the sensitive fern, Onoclea sensibilis L. By differential hybridization with labeled probes made to poly(A)+ RNA of spores, gametophytes and leaves, two spore-specific clones (pOSS68 and pOSS194) were selected and characterized. Northern blot analysis showed that RNA sequences homologous to the two cDNA clones first appear in the post-meiotic spore and increase in abundance during spore maturity. Both RNA sequences decay during photoinduced germination of the spores and do not reappear in the gametophytes. In spores imbibed in the dark under conditions which do not favor germination, no significant decrease in pOSS194-mRNA abundance is noted. In contrast, the decrease in pOSS68 mRNA in dark-imbibed spores parallels that observed in photoinduced spores. The predicted amino-acid sequence of pOSS194 has a striking similarity to the early light-inducible proteins expressed during the greening of etiolated pea and barley seedlings, whereas that of pOSS68 shows some homology to proteins encoded by late-embryogenesis-abundant mRNAs of angiosperm embryos.</abstract><subject lang="en"><genre>Key words</genre><topic>Onoclea (cDNA library)</topic><topic>Light and development</topic><topic>Protein (late embryogenesis abundant)</topic><topic>Protein (early light-inducible)</topic><topic>Spore-specific mRNA</topic></subject><subject><genre>Abbreviations</genre><topic>bp : base pairs</topic><topic>cDNA : complementary DNA</topic><topic>ds : double-stranded</topic><topic>ELIP : early light-inducible proteins</topic><topic>LEA : late embryogenesis abundant</topic><topic>nt : nucleotide</topic><topic>ss : single stranded</topic></subject><relatedItem type="host"><titleInfo><title>Planta</title><subTitle>An International Journal of Plant Biology</subTitle></titleInfo><titleInfo type="abbreviated"><title>Planta</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">1993-01-01</dateIssued><copyrightDate encoding="w3cdtf">1993</copyrightDate></originInfo><subject><genre>Life Sciences</genre><topic>Agriculture</topic><topic>Ecology</topic><topic>Forestry</topic><topic>Plant Sciences</topic></subject><identifier type="ISSN">0032-0935</identifier><identifier type="eISSN">1432-2048</identifier><identifier type="JournalID">425</identifier><identifier type="IssueArticleCount">22</identifier><identifier type="VolumeIssueCount">4</identifier><part><date>1993</date><detail type="volume"><number>189</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="pages"><start>1</start><end>9</end></extent></part><recordInfo><recordOrigin>Springer-Verlag, 1993</recordOrigin></recordInfo></relatedItem><identifier type="istex">465FA5385D2D8E7464733263B678C4A16B418EB5</identifier><identifier type="ark">ark:/67375/1BB-TL77DVRT-Z</identifier><identifier type="DOI">10.1007/BF00201336</identifier><identifier type="ArticleID">BF00201336</identifier><identifier type="ArticleID">Art1</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer-Verlag, 1993</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Springer-Verlag, 1993</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/1BB-TL77DVRT-Z/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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