GAL4 activates gene expression in mammalian cells
Identifieur interne : 001736 ( Istex/Corpus ); précédent : 001735; suivant : 001737GAL4 activates gene expression in mammalian cells
Auteurs : Hitoshi Kakidani ; Mark PtashneSource :
- Cell [ 0092-8674 ] ; 1988.
English descriptors
- Teeft :
- 1fmer, Activates, Activates gene expression, Activates transcription, Activator, Amino, Amino acids, Biol, Deletion, Deletion derivatives, Derivative, Dexamethasone, Effector, Effector plasmid, Effector plasmids, Gal4, Gal4 binding site, Galactose, Gene expression, Giniger, Glucocorticoid, Glucocorticoid receptor, Gres, Late promoter, Mammalian cells, Mmtv, Mmtv promoter, Pgmcs, Plasmid, Promoter, Ptashne, Receptor, Reference plasmid, Reporter plasmid, Reporter plasmids, Transcription, Transcriptional, Transfected, Tumor virus, Uaso, Yamamoto, Yeast.
Abstract
Abstract: GAL4, a protein that activates transcription in yeast, is shown to activate the mouse mammary tumor virus promoter in mammalian cells. Activation depends upon a GAL4-binding sequence inserted upstream of the gene. Deletion mutants of GAL4 bearing one or both of the “activating regions” required for activation in yeast also activate transcription in mammalian cells. A derivative of GAL4 that binds to DNA but cannot activate transcription in yeast also fails to activate transcription in mammalian cells. We also show that GAL4 and the glucocorticoid receptor activate the mouse mammary tumor virus promoter synergistically.
Url:
DOI: 10.1016/0092-8674(88)90504-1
Links to Exploration step
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<front><div type="abstract" xml:lang="en">Abstract: GAL4, a protein that activates transcription in yeast, is shown to activate the mouse mammary tumor virus promoter in mammalian cells. Activation depends upon a GAL4-binding sequence inserted upstream of the gene. Deletion mutants of GAL4 bearing one or both of the “activating regions” required for activation in yeast also activate transcription in mammalian cells. A derivative of GAL4 that binds to DNA but cannot activate transcription in yeast also fails to activate transcription in mammalian cells. We also show that GAL4 and the glucocorticoid receptor activate the mouse mammary tumor virus promoter synergistically.</div>
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<abstract xml:lang="en"><p>Abstract: GAL4, a protein that activates transcription in yeast, is shown to activate the mouse mammary tumor virus promoter in mammalian cells. Activation depends upon a GAL4-binding sequence inserted upstream of the gene. Deletion mutants of GAL4 bearing one or both of the “activating regions” required for activation in yeast also activate transcription in mammalian cells. A derivative of GAL4 that binds to DNA but cannot activate transcription in yeast also fails to activate transcription in mammalian cells. We also show that GAL4 and the glucocorticoid receptor activate the mouse mammary tumor virus promoter synergistically.</p>
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