Infectious eggplant mosaic tymovirus and ononis yellow mosaic tymovirus from cloned cDNA
Identifieur interne : 001144 ( Istex/Corpus ); précédent : 001143; suivant : 001145Infectious eggplant mosaic tymovirus and ononis yellow mosaic tymovirus from cloned cDNA
Auteurs : M. L. Skotnicki ; S. W. Ding ; A. M. Mackenzie ; A. J. GibbsSource :
- Archives of Virology [ 0304-8608 ] ; 1993-03-01.
English descriptors
- Teeft :
- Cdna, Cdna clone, Cdna synthesis, Clone, Diii, Dsdna, Eggplant, Eggplant mosaic tymovirus, Eggplant mosaic virus, Encoding, Endonuclease, First strand primer, Genome, Genomic, Glutinosa, Glutinosa seedlings, Host range determination, Host range differences, Hybrid, Hydrolysed, Infectious clones, Inoculated, Italic, Ligated, Linearized, Molecular basis, Mosaic, Mosaic tymovirus, Mosaic virus, Nonviral, Nonviral nucleotides, Nucleic, Nucleic acids, Nucleotide, Nucleotide sequence, Ononis, Oymv, Oymv genome, Pbluescript, Plants inoculated, Plasmid, Polymerase, Primer, Restriction endonucleases, Seedling, Skotnicki, Smaller plasmids, States biochemicals, Systemic symptoms, Terminus, Turnip, Tymovirus, Tymoviruses, Tymv, Vector hydrolysed, Viral, Viral genome, Viral genomes, Viral sequence, Viral terminus, Virion, Virion protein gene, Virion protein genes.
Abstract
Summary: Eggplant mosaic virus (EMV) and ononis yellow mosaic virus (OYMV) are two tymoviruses that have ssRNA genomes of about 6.2 kb and 6.3 kb, and which infect solanaceous and leguminous hosts, respectively. Full-length cDNA clones of these viruses were constructed with a T 7 promoter adjacent to the 5′ terminus of the DNA copy of the viral genome, and with unique restriction endonuclease sites at the 3′ terminus. This allowed RNA to be transcribed from the DNA encoding the genome. The transcript RNA was infectious when inoculated toNicotiana glutinosa (for EMV) andPisum sativum (for OYMV). These clones, together with clones of turnip yellow mosaic tymovirus, which infects brassicas, have been used to construct hybrids in which the virion protein gene was exchanged between EMV or OYMV and turnip yellow mosaic virus. These and other hybrids are being used to investigate the molecular basis for host range differences in tymoviruses.
Url:
DOI: 10.1007/BF01379079
Links to Exploration step
ISTEX:2D30324D03D610AEEA4F7436DF6FB1A6BA7CFB57Le document en format XML
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Full-length cDNA clones of these viruses were constructed with a T 7 promoter adjacent to the 5′ terminus of the DNA copy of the viral genome, and with unique restriction endonuclease sites at the 3′ terminus. This allowed RNA to be transcribed from the DNA encoding the genome. The transcript RNA was infectious when inoculated toNicotiana glutinosa (for EMV) andPisum sativum (for OYMV). These clones, together with clones of turnip yellow mosaic tymovirus, which infects brassicas, have been used to construct hybrids in which the virion protein gene was exchanged between EMV or OYMV and turnip yellow mosaic virus. 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Gibbs</name><affiliations><json:string>Molecular Evolution and Systematics, Research School of Biological Sciences, Australian National University, Canberra, ACT, Australia</json:string></affiliations></json:item></author><articleId><json:string>BF01379079</json:string><json:string>Art5</json:string></articleId><arkIstex>ark:/67375/1BB-JNNTVVNF-2</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Summary: Eggplant mosaic virus (EMV) and ononis yellow mosaic virus (OYMV) are two tymoviruses that have ssRNA genomes of about 6.2 kb and 6.3 kb, and which infect solanaceous and leguminous hosts, respectively. Full-length cDNA clones of these viruses were constructed with a T 7 promoter adjacent to the 5′ terminus of the DNA copy of the viral genome, and with unique restriction endonuclease sites at the 3′ terminus. This allowed RNA to be transcribed from the DNA encoding the genome. The transcript RNA was infectious when inoculated toNicotiana glutinosa (for EMV) andPisum sativum (for OYMV). These clones, together with clones of turnip yellow mosaic tymovirus, which infects brassicas, have been used to construct hybrids in which the virion protein gene was exchanged between EMV or OYMV and turnip yellow mosaic virus. These and other hybrids are being used to investigate the molecular basis for host range differences in tymoviruses.</abstract><qualityIndicators><score>8.8</score><pdfWordCount>5601</pdfWordCount><pdfCharCount>30957</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>14</pdfPageCount><pdfPageSize>562 x 774 pts</pdfPageSize><refBibsNative>false</refBibsNative><abstractWordCount>150</abstractWordCount><abstractCharCount>948</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Infectious eggplant mosaic tymovirus and ononis yellow mosaic tymovirus from cloned cDNA</title><pmid><json:string>8328917</json:string></pmid><genre><json:string>research-article</json:string></genre><host><title>Archives of Virology</title><language><json:string>unknown</json:string></language><publicationDate>1993</publicationDate><copyrightDate>1993</copyrightDate><issn><json:string>0304-8608</json:string></issn><eissn><json:string>1432-8798</json:string></eissn><journalId><json:string>705</json:string></journalId><volume>131</volume><issue>1-2</issue><pages><first>47</first><last>60</last></pages><genre><json:string>journal</json:string></genre><subject><json:item><value>Medical Microbiology</value></json:item><json:item><value>Virology</value></json:item><json:item><value>Infectious Diseases</value></json:item></subject></host><namedEntities><unitex><date></date><geogName></geogName><orgName></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName></persName><placeName></placeName><ref_url></ref_url><ref_bibl></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/1BB-JNNTVVNF-2</json:string></ark><categories><wos><json:string>1 - 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Full-length cDNA clones of these viruses were constructed with a T 7 promoter adjacent to the 5′ terminus of the DNA copy of the viral genome, and with unique restriction endonuclease sites at the 3′ terminus. This allowed RNA to be transcribed from the DNA encoding the genome. The transcript RNA was infectious when inoculated toNicotiana glutinosa (for EMV) andPisum sativum (for OYMV). These clones, together with clones of turnip yellow mosaic tymovirus, which infects brassicas, have been used to construct hybrids in which the virion protein gene was exchanged between EMV or OYMV and turnip yellow mosaic virus. These and other hybrids are being used to investigate the molecular basis for host range differences in tymoviruses.</p></abstract><textClass><keywords scheme="Journal Subject"><list><head>Biomedicine</head><item><term>Medical Microbiology</term></item><item><term>Virology</term></item><item><term>Infectious Diseases</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1992-11-09">Created</change><change when="1993-03-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/1BB-JNNTVVNF-2/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus springer-journals not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" 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TocLevels="0"><VolumeIDStart>131</VolumeIDStart><VolumeIDEnd>131</VolumeIDEnd><VolumeIssueCount>4</VolumeIssueCount></VolumeInfo><Issue IssueType="Combined"><IssueInfo TocLevels="0"><IssueIDStart>1</IssueIDStart><IssueIDEnd>2</IssueIDEnd><IssueArticleCount>22</IssueArticleCount><IssueHistory><CoverDate><DateString>1993</DateString><Year>1993</Year><Month>3</Month></CoverDate></IssueHistory><IssueCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>1993</CopyrightYear></IssueCopyright></IssueInfo><Article ID="Art5"><ArticleInfo Language="En" ArticleType="OriginalPaper" NumberingStyle="Unnumbered" TocLevels="0" ContainsESM="No"><ArticleID>BF01379079</ArticleID><ArticleDOI>10.1007/BF01379079</ArticleDOI><ArticleSequenceNumber>5</ArticleSequenceNumber><ArticleTitle Language="En">Infectious eggplant mosaic tymovirus and ononis yellow mosaic tymovirus from cloned cDNA</ArticleTitle><ArticleCategory>Original Papers</ArticleCategory><ArticleFirstPage>47</ArticleFirstPage><ArticleLastPage>60</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2005</Year><Month>3</Month><Day>11</Day></RegistrationDate><Received><Year>1992</Year><Month>11</Month><Day>9</Day></Received><Accepted><Year>1993</Year><Month>1</Month><Day>11</Day></Accepted></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>1993</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants><ArticleContext><JournalID>705</JournalID><VolumeIDStart>131</VolumeIDStart><VolumeIDEnd>131</VolumeIDEnd><IssueIDStart>1</IssueIDStart><IssueIDEnd>2</IssueIDEnd></ArticleContext></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>M.</GivenName><GivenName>L.</GivenName><FamilyName>Skotnicki</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>S.</GivenName><GivenName>-W.</GivenName><FamilyName>Ding</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>A.</GivenName><GivenName>M.</GivenName><FamilyName>Mackenzie</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>A.</GivenName><GivenName>J.</GivenName><FamilyName>Gibbs</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgDivision>Molecular Evolution and Systematics, Research School of Biological Sciences</OrgDivision><OrgName>Australian National University</OrgName><OrgAddress><City>Canberra</City><State>ACT</State><Country>Australia</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Summary</Heading><Para>Eggplant mosaic virus (EMV) and ononis yellow mosaic virus (OYMV) are two tymoviruses that have ssRNA genomes of about 6.2 kb and 6.3 kb, and which infect solanaceous and leguminous hosts, respectively. Full-length cDNA clones of these viruses were constructed with a T 7 promoter adjacent to the 5′ terminus of the DNA copy of the viral genome, and with unique restriction endonuclease sites at the 3′ terminus. This allowed RNA to be transcribed from the DNA encoding the genome. The transcript RNA was infectious when inoculated to<Emphasis Type="Italic">Nicotiana glutinosa</Emphasis> (for EMV) and<Emphasis Type="Italic">Pisum sativum</Emphasis> (for OYMV). These clones, together with clones of turnip yellow mosaic tymovirus, which infects brassicas, have been used to construct hybrids in which the virion protein gene was exchanged between EMV or OYMV and turnip yellow mosaic virus. These and other hybrids are being used to investigate the molecular basis for host range differences in tymoviruses.</Para></Abstract></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Infectious eggplant mosaic tymovirus and ononis yellow mosaic tymovirus from cloned cDNA</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Infectious eggplant mosaic tymovirus and ononis yellow mosaic tymovirus from cloned cDNA</title></titleInfo><name type="personal"><namePart type="given">M.</namePart><namePart type="given">L.</namePart><namePart type="family">Skotnicki</namePart><affiliation>Molecular Evolution and Systematics, Research School of Biological Sciences, Australian National University, Canberra, ACT, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="given">-W.</namePart><namePart type="family">Ding</namePart><affiliation>Molecular Evolution and Systematics, Research School of Biological Sciences, Australian National University, Canberra, ACT, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="given">M.</namePart><namePart type="family">Mackenzie</namePart><affiliation>Molecular Evolution and Systematics, Research School of Biological Sciences, Australian National University, Canberra, ACT, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="given">J.</namePart><namePart type="family">Gibbs</namePart><affiliation>Molecular Evolution and Systematics, Research School of Biological Sciences, Australian National University, Canberra, ACT, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer-Verlag</publisher><place><placeTerm type="text">Vienna</placeTerm></place><dateCreated encoding="w3cdtf">1992-11-09</dateCreated><dateIssued encoding="w3cdtf">1993-03-01</dateIssued><copyrightDate encoding="w3cdtf">1993</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Summary: Eggplant mosaic virus (EMV) and ononis yellow mosaic virus (OYMV) are two tymoviruses that have ssRNA genomes of about 6.2 kb and 6.3 kb, and which infect solanaceous and leguminous hosts, respectively. Full-length cDNA clones of these viruses were constructed with a T 7 promoter adjacent to the 5′ terminus of the DNA copy of the viral genome, and with unique restriction endonuclease sites at the 3′ terminus. This allowed RNA to be transcribed from the DNA encoding the genome. The transcript RNA was infectious when inoculated toNicotiana glutinosa (for EMV) andPisum sativum (for OYMV). These clones, together with clones of turnip yellow mosaic tymovirus, which infects brassicas, have been used to construct hybrids in which the virion protein gene was exchanged between EMV or OYMV and turnip yellow mosaic virus. These and other hybrids are being used to investigate the molecular basis for host range differences in tymoviruses.</abstract><note>Original Papers</note><relatedItem type="host"><titleInfo><title>Archives of Virology</title></titleInfo><titleInfo type="abbreviated"><title>Archives of Virology</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">1993-03-01</dateIssued><copyrightDate encoding="w3cdtf">1993</copyrightDate></originInfo><subject><genre>Biomedicine</genre><topic>Medical Microbiology</topic><topic>Virology</topic><topic>Infectious Diseases</topic></subject><identifier type="ISSN">0304-8608</identifier><identifier type="eISSN">1432-8798</identifier><identifier type="JournalID">705</identifier><identifier type="IssueArticleCount">22</identifier><identifier type="VolumeIssueCount">4</identifier><part><date>1993</date><detail type="volume"><number>131</number><caption>vol.</caption></detail><detail type="issue"><number>1-2</number><caption>no.</caption></detail><extent unit="pages"><start>47</start><end>60</end></extent></part><recordInfo><recordOrigin>Springer-Verlag, 1993</recordOrigin></recordInfo></relatedItem><identifier type="istex">2D30324D03D610AEEA4F7436DF6FB1A6BA7CFB57</identifier><identifier type="ark">ark:/67375/1BB-JNNTVVNF-2</identifier><identifier type="DOI">10.1007/BF01379079</identifier><identifier type="ArticleID">BF01379079</identifier><identifier type="ArticleID">Art5</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer-Verlag, 1993</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Springer-Verlag, 1993</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/1BB-JNNTVVNF-2/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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