Estrogen-inducible GFP expression patterns in rice ( Oryza sativa L.)
Identifieur interne : 001143 ( Istex/Corpus ); précédent : 001142; suivant : 001144Estrogen-inducible GFP expression patterns in rice ( Oryza sativa L.)
Auteurs : Ayako Okuzaki ; Ken-Ichi Konagaya ; Yoshihiko Nanasato ; Mai Tsuda ; Yutaka TabeiSource :
- Plant Cell Reports [ 0721-7714 ] ; 2011-04-01.
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Abstract
Abstract: We investigated estrogen-inducible green fluorescent protein (GFP) expression patterns using an estrogen receptor fused chimeric transcription activator, XVE, in the monocotyledonous model plant rice (Oryza sativa L.). This system has been shown to be an effective chemical-inducible gene expression system in Arabidopsis and has been applied to other plants in order to investigate gene functions or produce marker-free transgenic plants. However, limited information is available on the correlation between inducer concentration and the expression level of the gene induced in monocots. Here, we produced a transgenic rice integrated estrogen-inducible GFP expression vector, pLex:GFP, and investigated dose–response and time-course patterns of GFP induction in rice calli and seedlings for the first time. With 17-β-estradiol treatment at >5 μM, GFP signals were detected in the entire surface of calli within 2 days of culture. Highest GFP signals were extended for 8 days with estradiol treatment at 25 μM. In three-leaf-stage seedlings, GFP signals in the leaves of pLex:GFP-integrated transgenic lines were weaker than those in the leaves of p35S:GFP-integrated transgenic lines. However, GFP signals in the roots of pLex:GFP- and p35S:GFP-integrated transgenic lines were similar with estradiol treatment at >10 μM. With regard to controlling appropriate gene expression, these results might provide helpful indications on estradiol treatment conditions to be used for the XVE system in rice and other monocots.
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DOI: 10.1007/s00299-010-0963-0
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Japan</mods:affiliation></affiliation></author><author><name sortKey="Tsuda, Mai" sort="Tsuda, Mai" uniqKey="Tsuda M" first="Mai" last="Tsuda">Mai Tsuda</name><affiliation><mods:affiliation>Division of Plant Sciences, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, 305-8602, Tsukuba, Ibaraki, Japan</mods:affiliation></affiliation></author><author><name sortKey="Tabei, Yutaka" sort="Tabei, Yutaka" uniqKey="Tabei Y" first="Yutaka" last="Tabei">Yutaka Tabei</name><affiliation><mods:affiliation>Division of Plant Sciences, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, 305-8602, Tsukuba, Ibaraki, Japan</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: tabei@affrc.go.jp</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Plant Cell Reports</title><title level="j" type="abbrev">Plant Cell Rep</title><idno type="ISSN">0721-7714</idno><idno type="eISSN">1432-203X</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="2011-04-01">2011-04-01</date><biblScope unit="volume">30</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="529">529</biblScope><biblScope unit="page" to="538">538</biblScope></imprint><idno type="ISSN">0721-7714</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0721-7714</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Estradiol</term><term>Estrogen inducible</term><term>GFP</term><term>Rice</term><term>XVE system</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: We investigated estrogen-inducible green fluorescent protein (GFP) expression patterns using an estrogen receptor fused chimeric transcription activator, XVE, in the monocotyledonous model plant rice (Oryza sativa L.). This system has been shown to be an effective chemical-inducible gene expression system in Arabidopsis and has been applied to other plants in order to investigate gene functions or produce marker-free transgenic plants. However, limited information is available on the correlation between inducer concentration and the expression level of the gene induced in monocots. Here, we produced a transgenic rice integrated estrogen-inducible GFP expression vector, pLex:GFP, and investigated dose–response and time-course patterns of GFP induction in rice calli and seedlings for the first time. With 17-β-estradiol treatment at >5 μM, GFP signals were detected in the entire surface of calli within 2 days of culture. Highest GFP signals were extended for 8 days with estradiol treatment at 25 μM. In three-leaf-stage seedlings, GFP signals in the leaves of pLex:GFP-integrated transgenic lines were weaker than those in the leaves of p35S:GFP-integrated transgenic lines. However, GFP signals in the roots of pLex:GFP- and p35S:GFP-integrated transgenic lines were similar with estradiol treatment at >10 μM. With regard to controlling appropriate gene expression, these results might provide helpful indications on estradiol treatment conditions to be used for the XVE system in rice and other monocots.</div></front></TEI><istex><corpusName>springer-journals</corpusName><author><json:item><name>Ayako Okuzaki</name><affiliations><json:string>Division of Plant Sciences, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, 305-8602, Tsukuba, Ibaraki, Japan</json:string></affiliations></json:item><json:item><name>Ken-ichi Konagaya</name><affiliations><json:string>Division of Plant Sciences, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, 305-8602, Tsukuba, Ibaraki, Japan</json:string><json:string>Forest Bio-Research Laboratory 1, Forest Bio-Research Center, Forestry and Forest Products Research Institute, 3809-1 Ishi, Juo, 319-1301, Hitachi, Ibaraki, Japan</json:string></affiliations></json:item><json:item><name>Yoshihiko Nanasato</name><affiliations><json:string>Division of Plant Sciences, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, 305-8602, Tsukuba, Ibaraki, Japan</json:string></affiliations></json:item><json:item><name>Mai Tsuda</name><affiliations><json:string>Division of Plant Sciences, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, 305-8602, Tsukuba, Ibaraki, Japan</json:string></affiliations></json:item><json:item><name>Yutaka Tabei</name><affiliations><json:string>Division of Plant Sciences, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, 305-8602, Tsukuba, Ibaraki, Japan</json:string><json:string>E-mail: tabei@affrc.go.jp</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Estrogen inducible</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>XVE system</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Estradiol</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Rice</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>GFP</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>GFP : Green fluorescent protein</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Estradiol : 17-β-Estradiol</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>hpt : Hygromycin phosphotransferase</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PCR : Polymerase chain reaction</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>XVE : An estrogen receptor fused chimeric transcription activator</value></json:item></subject><articleId><json:string>963</json:string><json:string>s00299-010-0963-0</json:string></articleId><arkIstex>ark:/67375/VQC-DSZGX0S2-1</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Abstract: We investigated estrogen-inducible green fluorescent protein (GFP) expression patterns using an estrogen receptor fused chimeric transcription activator, XVE, in the monocotyledonous model plant rice (Oryza sativa L.). This system has been shown to be an effective chemical-inducible gene expression system in Arabidopsis and has been applied to other plants in order to investigate gene functions or produce marker-free transgenic plants. However, limited information is available on the correlation between inducer concentration and the expression level of the gene induced in monocots. Here, we produced a transgenic rice integrated estrogen-inducible GFP expression vector, pLex:GFP, and investigated dose–response and time-course patterns of GFP induction in rice calli and seedlings for the first time. With 17-β-estradiol treatment at >5 μM, GFP signals were detected in the entire surface of calli within 2 days of culture. Highest GFP signals were extended for 8 days with estradiol treatment at 25 μM. In three-leaf-stage seedlings, GFP signals in the leaves of pLex:GFP-integrated transgenic lines were weaker than those in the leaves of p35S:GFP-integrated transgenic lines. However, GFP signals in the roots of pLex:GFP- and p35S:GFP-integrated transgenic lines were similar with estradiol treatment at >10 μM. With regard to controlling appropriate gene expression, these results might provide helpful indications on estradiol treatment conditions to be used for the XVE system in rice and other monocots.</abstract><qualityIndicators><refBibsNative>false</refBibsNative><abstractWordCount>214</abstractWordCount><abstractCharCount>1529</abstractCharCount><keywordCount>10</keywordCount><score>9.568</score><pdfWordCount>5760</pdfWordCount><pdfCharCount>35245</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>10</pdfPageCount><pdfPageSize>595.276 x 790.866 pts</pdfPageSize></qualityIndicators><title>Estrogen-inducible GFP expression patterns in rice ( Oryza sativa L.)</title><genre><json:string>research-article</json:string></genre><host><title>Plant Cell 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type="first">Mai</forename><surname>Tsuda</surname></persName><affiliation>Division of Plant Sciences, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, 305-8602, Tsukuba, Ibaraki, Japan</affiliation></author><author xml:id="author-0004" corresp="yes"><persName><forename type="first">Yutaka</forename><surname>Tabei</surname></persName><email>tabei@affrc.go.jp</email><affiliation>Division of Plant Sciences, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, 305-8602, Tsukuba, Ibaraki, Japan</affiliation></author><idno type="istex">D073EDBCEF12DCF86E99393D2E9ED38E9FDF70D3</idno><idno type="ark">ark:/67375/VQC-DSZGX0S2-1</idno><idno type="DOI">10.1007/s00299-010-0963-0</idno><idno type="article-id">963</idno><idno type="article-id">s00299-010-0963-0</idno></analytic><monogr><title level="j">Plant Cell Reports</title><title level="j" type="abbrev">Plant Cell Rep</title><idno type="pISSN">0721-7714</idno><idno type="eISSN">1432-203X</idno><idno 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This system has been shown to be an effective chemical-inducible gene expression system in Arabidopsis and has been applied to other plants in order to investigate gene functions or produce marker-free transgenic plants. However, limited information is available on the correlation between inducer concentration and the expression level of the gene induced in monocots. Here, we produced a transgenic rice integrated estrogen-inducible GFP expression vector, pLex:GFP, and investigated dose–response and time-course patterns of GFP induction in rice calli and seedlings for the first time. With 17-β-estradiol treatment at >5 μM, GFP signals were detected in the entire surface of calli within 2 days of culture. Highest GFP signals were extended for 8 days with estradiol treatment at 25 μM. In three-leaf-stage seedlings, GFP signals in the leaves of pLex:GFP-integrated transgenic lines were weaker than those in the leaves of p35S:GFP-integrated transgenic lines. However, GFP signals in the roots of pLex:GFP- and p35S:GFP-integrated transgenic lines were similar with estradiol treatment at >10 μM. With regard to controlling appropriate gene expression, these results might provide helpful indications on estradiol treatment conditions to be used for the XVE system in rice and other monocots.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Estrogen inducible</term></item><item><term>XVE system</term></item><item><term>Estradiol</term></item><item><term>Rice</term></item><item><term>GFP</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list><head>Abbreviations</head><item><term>GFP</term><term>Green fluorescent protein</term></item><item><term>Estradiol</term><term>17-β-Estradiol</term></item><item><term>hpt</term><term>Hygromycin phosphotransferase</term></item><item><term>PCR</term><term>Polymerase chain reaction</term></item><item><term>XVE</term><term>An estrogen receptor fused chimeric transcription activator</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>Life Sciences</head><item><term>Cell Biology</term></item><item><term>Plant Biochemistry</term></item><item><term>Plant Sciences</term></item><item><term>Biotechnology</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2010-09-13">Created</change><change when="2011-04-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-DSZGX0S2-1/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus springer-journals not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" name="istex:docType"></istex:docType><istex:document><Publisher><PublisherInfo><PublisherName>Springer-Verlag</PublisherName><PublisherLocation>Berlin/Heidelberg</PublisherLocation></PublisherInfo><Journal OutputMedium="All"><JournalInfo JournalProductType="ArchiveJournal" NumberingStyle="Unnumbered"><JournalID>299</JournalID><JournalPrintISSN>0721-7714</JournalPrintISSN><JournalElectronicISSN>1432-203X</JournalElectronicISSN><JournalTitle>Plant Cell Reports</JournalTitle><JournalAbbreviatedTitle>Plant Cell Rep</JournalAbbreviatedTitle><JournalSubjectGroup><JournalSubject Type="Primary">Life Sciences</JournalSubject><JournalSubject Type="Secondary">Cell Biology</JournalSubject><JournalSubject Type="Secondary">Plant Biochemistry</JournalSubject><JournalSubject Type="Secondary">Plant Sciences </JournalSubject><JournalSubject Type="Secondary">Biotechnology</JournalSubject></JournalSubjectGroup></JournalInfo><Volume OutputMedium="All"><VolumeInfo TocLevels="0" VolumeType="Regular"><VolumeIDStart>30</VolumeIDStart><VolumeIDEnd>30</VolumeIDEnd><VolumeIssueCount>12</VolumeIssueCount></VolumeInfo><Issue IssueType="Regular" OutputMedium="All"><IssueInfo IssueType="Regular" TocLevels="0"><IssueIDStart>4</IssueIDStart><IssueIDEnd>4</IssueIDEnd><IssueArticleCount>20</IssueArticleCount><IssueHistory><OnlineDate><Year>2011</Year><Month>3</Month><Day>15</Day></OnlineDate><PrintDate><Year>2011</Year><Month>3</Month><Day>14</Day></PrintDate><CoverDate><Year>2011</Year><Month>4</Month></CoverDate><PricelistYear>2011</PricelistYear></IssueHistory><IssueCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>2011</CopyrightYear></IssueCopyright></IssueInfo><Article ID="s00299-010-0963-0" OutputMedium="All"><ArticleInfo ArticleType="OriginalPaper" ContainsESM="No" Language="En" NumberingStyle="Unnumbered" TocLevels="0"><ArticleID>963</ArticleID><ArticleDOI>10.1007/s00299-010-0963-0</ArticleDOI><ArticleSequenceNumber>8</ArticleSequenceNumber><ArticleTitle Language="En" OutputMedium="All">Estrogen-inducible GFP expression patterns in rice (<Emphasis Type="Italic">Oryza sativa</Emphasis> L.)</ArticleTitle><ArticleCategory>Original Paper</ArticleCategory><ArticleFirstPage>529</ArticleFirstPage><ArticleLastPage>538</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2010</Year><Month>11</Month><Day>24</Day></RegistrationDate><Received><Year>2010</Year><Month>9</Month><Day>13</Day></Received><Revised><Year>2010</Year><Month>11</Month><Day>5</Day></Revised><Accepted><Year>2010</Year><Month>11</Month><Day>23</Day></Accepted><OnlineDate><Year>2010</Year><Month>12</Month><Day>8</Day></OnlineDate></ArticleHistory><ArticleEditorialResponsibility>K. Toriyama</ArticleEditorialResponsibility><ArticleCopyright><CopyrightHolderName>The Author(s)</CopyrightHolderName><CopyrightYear>2010</CopyrightYear></ArticleCopyright><ArticleGrants Type="OpenChoice"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="OpenAccess"></BodyPDFGrant><BodyHTMLGrant Grant="OpenAccess"></BodyHTMLGrant><BibliographyGrant Grant="OpenAccess"></BibliographyGrant><ESMGrant Grant="OpenAccess"></ESMGrant></ArticleGrants></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Ayako</GivenName><FamilyName>Okuzaki</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff2" PresentAffiliationID="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Ken-ichi</GivenName><FamilyName>Konagaya</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Yoshihiko</GivenName><FamilyName>Nanasato</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Mai</GivenName><FamilyName>Tsuda</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Yutaka</GivenName><FamilyName>Tabei</FamilyName></AuthorName><Contact><Phone>+81-29-8388372</Phone><Fax>+81-29-8388372</Fax><Email>tabei@affrc.go.jp</Email></Contact></Author><Affiliation ID="Aff1"><OrgDivision>Division of Plant Sciences</OrgDivision><OrgName>National Institute of Agrobiological Sciences</OrgName><OrgAddress><Street>2-1-2 Kannondai</Street><City>Tsukuba</City><State>Ibaraki</State><Postcode>305-8602</Postcode><Country Code="JP">Japan</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>Forest Bio-Research Laboratory 1</OrgDivision><OrgName>Forest Bio-Research Center, Forestry and Forest Products Research Institute</OrgName><OrgAddress><Street>3809-1 Ishi, Juo</Street><City>Hitachi</City><State>Ibaraki</State><Postcode>319-1301</Postcode><Country Code="JP">Japan</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En" OutputMedium="All"><Heading>Abstract</Heading><Para>We investigated estrogen-inducible green fluorescent protein (GFP) expression patterns using an estrogen receptor fused chimeric transcription activator, XVE, in the monocotyledonous model plant rice (<Emphasis Type="Italic">Oryza sativa</Emphasis> L<Emphasis Type="Italic">.</Emphasis>). This system has been shown to be an effective chemical-inducible gene expression system in <Emphasis Type="Italic">Arabidopsis</Emphasis> and has been applied to other plants in order to investigate gene functions or produce marker-free transgenic plants. However, limited information is available on the correlation between inducer concentration and the expression level of the gene induced in monocots. Here, we produced a transgenic rice integrated estrogen-inducible GFP expression vector, <Emphasis Type="Italic">pLex:GFP</Emphasis>, and investigated dose–response and time-course patterns of GFP induction in rice calli and seedlings for the first time. With 17-β-estradiol treatment at >5 μM, GFP signals were detected in the entire surface of calli within 2 days of culture. Highest GFP signals were extended for 8 days with estradiol treatment at 25 μM. In three-leaf-stage seedlings, GFP signals in the leaves of <Emphasis Type="Italic">pLex:GFP</Emphasis>-integrated transgenic lines were weaker than those in the leaves of <Emphasis Type="Italic">p35S:GFP</Emphasis>-integrated transgenic lines. However, GFP signals in the roots of <Emphasis Type="Italic">pLex:GFP</Emphasis>- and <Emphasis Type="Italic">p35S:GFP</Emphasis>-integrated transgenic lines were similar with estradiol treatment at >10 μM. With regard to controlling appropriate gene expression, these results might provide helpful indications on estradiol treatment conditions to be used for the XVE system in rice and other monocots.</Para></Abstract><KeywordGroup Language="En" OutputMedium="All"><Heading>Keywords</Heading><Keyword>Estrogen inducible</Keyword><Keyword>XVE system</Keyword><Keyword>Estradiol</Keyword><Keyword>Rice</Keyword><Keyword>GFP</Keyword></KeywordGroup><AbbreviationGroup><Heading>Abbreviations</Heading><DefinitionList><DefinitionListEntry><Term>GFP</Term><Description><Para>Green fluorescent protein</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>Estradiol</Term><Description><Para>17-β-Estradiol</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>hpt</Term><Description><Para>Hygromycin phosphotransferase</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>PCR</Term><Description><Para>Polymerase chain reaction</Para></Description></DefinitionListEntry><DefinitionListEntry><Term>XVE</Term><Description><Para>An estrogen receptor fused chimeric transcription activator</Para></Description></DefinitionListEntry></DefinitionList></AbbreviationGroup><ArticleNote Type="CommunicatedBy"><SimplePara>Communicated by K. Toriyama.</SimplePara></ArticleNote></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Estrogen-inducible GFP expression patterns in rice ( Oryza sativa L.)</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Estrogen-inducible GFP expression patterns in rice ( Oryza sativa L.)</title></titleInfo><name type="personal"><namePart type="given">Ayako</namePart><namePart type="family">Okuzaki</namePart><affiliation>Division of Plant Sciences, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, 305-8602, Tsukuba, Ibaraki, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ken-ichi</namePart><namePart type="family">Konagaya</namePart><affiliation>Division of Plant Sciences, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, 305-8602, Tsukuba, Ibaraki, Japan</affiliation><affiliation>Forest Bio-Research Laboratory 1, Forest Bio-Research Center, Forestry and Forest Products Research Institute, 3809-1 Ishi, Juo, 319-1301, Hitachi, Ibaraki, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yoshihiko</namePart><namePart type="family">Nanasato</namePart><affiliation>Division of Plant Sciences, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, 305-8602, Tsukuba, Ibaraki, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Mai</namePart><namePart type="family">Tsuda</namePart><affiliation>Division of Plant Sciences, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, 305-8602, Tsukuba, Ibaraki, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Yutaka</namePart><namePart type="family">Tabei</namePart><affiliation>Division of Plant Sciences, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, 305-8602, Tsukuba, Ibaraki, Japan</affiliation><affiliation>E-mail: tabei@affrc.go.jp</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer-Verlag</publisher><place><placeTerm type="text">Berlin/Heidelberg</placeTerm></place><dateCreated encoding="w3cdtf">2010-09-13</dateCreated><dateIssued encoding="w3cdtf">2011-04-01</dateIssued><copyrightDate encoding="w3cdtf">2010</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: We investigated estrogen-inducible green fluorescent protein (GFP) expression patterns using an estrogen receptor fused chimeric transcription activator, XVE, in the monocotyledonous model plant rice (Oryza sativa L.). This system has been shown to be an effective chemical-inducible gene expression system in Arabidopsis and has been applied to other plants in order to investigate gene functions or produce marker-free transgenic plants. However, limited information is available on the correlation between inducer concentration and the expression level of the gene induced in monocots. Here, we produced a transgenic rice integrated estrogen-inducible GFP expression vector, pLex:GFP, and investigated dose–response and time-course patterns of GFP induction in rice calli and seedlings for the first time. With 17-β-estradiol treatment at >5 μM, GFP signals were detected in the entire surface of calli within 2 days of culture. Highest GFP signals were extended for 8 days with estradiol treatment at 25 μM. In three-leaf-stage seedlings, GFP signals in the leaves of pLex:GFP-integrated transgenic lines were weaker than those in the leaves of p35S:GFP-integrated transgenic lines. However, GFP signals in the roots of pLex:GFP- and p35S:GFP-integrated transgenic lines were similar with estradiol treatment at >10 μM. With regard to controlling appropriate gene expression, these results might provide helpful indications on estradiol treatment conditions to be used for the XVE system in rice and other monocots.</abstract><note>Original Paper</note><subject lang="en"><genre>Keywords</genre><topic>Estrogen inducible</topic><topic>XVE system</topic><topic>Estradiol</topic><topic>Rice</topic><topic>GFP</topic></subject><subject><genre>Abbreviations</genre><topic>GFP : Green fluorescent protein</topic><topic>Estradiol : 17-β-Estradiol</topic><topic>hpt : Hygromycin phosphotransferase</topic><topic>PCR : Polymerase chain reaction</topic><topic>XVE : An estrogen receptor fused chimeric transcription activator</topic></subject><relatedItem type="host"><titleInfo><title>Plant Cell Reports</title></titleInfo><titleInfo type="abbreviated"><title>Plant Cell Rep</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">2011-03-15</dateIssued><copyrightDate encoding="w3cdtf">2011</copyrightDate></originInfo><subject><genre>Life Sciences</genre><topic>Cell Biology</topic><topic>Plant Biochemistry</topic><topic>Plant Sciences</topic><topic>Biotechnology</topic></subject><identifier type="ISSN">0721-7714</identifier><identifier type="eISSN">1432-203X</identifier><identifier type="JournalID">299</identifier><identifier type="IssueArticleCount">20</identifier><identifier type="VolumeIssueCount">12</identifier><part><date>2011</date><detail type="volume"><number>30</number><caption>vol.</caption></detail><detail type="issue"><number>4</number><caption>no.</caption></detail><extent unit="pages"><start>529</start><end>538</end></extent></part><recordInfo><recordOrigin>Springer-Verlag, 2011</recordOrigin></recordInfo></relatedItem><identifier type="istex">D073EDBCEF12DCF86E99393D2E9ED38E9FDF70D3</identifier><identifier type="ark">ark:/67375/VQC-DSZGX0S2-1</identifier><identifier type="DOI">10.1007/s00299-010-0963-0</identifier><identifier type="ArticleID">963</identifier><identifier type="ArticleID">s00299-010-0963-0</identifier><accessCondition type="use and reproduction" contentType="copyright">The Author(s), 2010</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>The Author(s), 2010</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-DSZGX0S2-1/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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