scpA , a new salicylate hydroxylase gene localized in salicylate/caprolactam degradation plasmids
Identifieur interne : 001112 ( Istex/Corpus ); précédent : 001111; suivant : 001113scpA , a new salicylate hydroxylase gene localized in salicylate/caprolactam degradation plasmids
Auteurs : A. V. Panov ; O. V. Volkova ; I. F. Puntus ; T. Z. Esikova ; I. A. Kosheleva ; A. M. BoroninSource :
- Molecular Biology [ 0026-8933 ] ; 2013-01-01.
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Abstract
Abstract: Both caprolactams and salicylate biodegradation by Pseudomonas salicylate/caprolactam degraders are controlled by large conjugative plasmids (SAL/CAP). Some of these plasmids have been assigned to the P-7 incompatibility group. The new salicylate 1-hydroxylase gene (scpA) has been detected in SAL/CAP plasmids and partially sequenced. The scpA gene was equally related to the closest homolog genes nahG (NAH7), salA (P. reinekei MT1), and nahU (pND6-1); however, the identity rate did not exceed 72–74%. The synthesis of salicylate 1-hydroxylase ScpA was not induced by salicylate. This enzyme had wide substrate specificity and exhibited the highest specific activity toward 4-methylsalicylate and nonsubstituted salicylate substrates. Furthermore, conjugative pseudomonads’ plasmids of salicylate degradation without the classical nah2 operon, which harbors only salicylate 1-hydroxylase gene nahU have been described for the first time.
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DOI: 10.1134/S0026893313010147
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">scpA , a new salicylate hydroxylase gene localized in salicylate/caprolactam degradation plasmids</title><author><name sortKey="Panov, A V" sort="Panov, A V" uniqKey="Panov A" first="A. V." last="Panov">A. V. Panov</name><affiliation><mods:affiliation>Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: panov_a_v@inbox.ru</mods:affiliation></affiliation></author><author><name sortKey="Volkova, O V" sort="Volkova, O V" uniqKey="Volkova O" first="O. V." last="Volkova">O. V. Volkova</name><affiliation><mods:affiliation>Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia</mods:affiliation></affiliation></author><author><name sortKey="Puntus, I F" sort="Puntus, I F" uniqKey="Puntus I" first="I. F." last="Puntus">I. F. Puntus</name><affiliation><mods:affiliation>Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia</mods:affiliation></affiliation></author><author><name sortKey="Esikova, T Z" sort="Esikova, T Z" uniqKey="Esikova T" first="T. Z." last="Esikova">T. Z. Esikova</name><affiliation><mods:affiliation>Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia</mods:affiliation></affiliation></author><author><name sortKey="Kosheleva, I A" sort="Kosheleva, I A" uniqKey="Kosheleva I" first="I. A." last="Kosheleva">I. A. 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V." last="Panov">A. V. Panov</name><affiliation><mods:affiliation>Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: panov_a_v@inbox.ru</mods:affiliation></affiliation></author><author><name sortKey="Volkova, O V" sort="Volkova, O V" uniqKey="Volkova O" first="O. V." last="Volkova">O. V. Volkova</name><affiliation><mods:affiliation>Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia</mods:affiliation></affiliation></author><author><name sortKey="Puntus, I F" sort="Puntus, I F" uniqKey="Puntus I" first="I. F." last="Puntus">I. F. Puntus</name><affiliation><mods:affiliation>Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia</mods:affiliation></affiliation></author><author><name sortKey="Esikova, T Z" sort="Esikova, T Z" uniqKey="Esikova T" first="T. Z." last="Esikova">T. Z. Esikova</name><affiliation><mods:affiliation>Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia</mods:affiliation></affiliation></author><author><name sortKey="Kosheleva, I A" sort="Kosheleva, I A" uniqKey="Kosheleva I" first="I. A." last="Kosheleva">I. A. Kosheleva</name><affiliation><mods:affiliation>Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia</mods:affiliation></affiliation><affiliation><mods:affiliation>Pushchino State Educational Institute of Natural Sciences, 142290, Pushchino, Russia</mods:affiliation></affiliation></author><author><name sortKey="Boronin, A M" sort="Boronin, A M" uniqKey="Boronin A" first="A. M." last="Boronin">A. M. Boronin</name><affiliation><mods:affiliation>Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia</mods:affiliation></affiliation><affiliation><mods:affiliation>Pushchino State Educational Institute of Natural Sciences, 142290, Pushchino, Russia</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Molecular Biology</title><title level="j" type="abbrev">Mol Biol</title><idno type="ISSN">0026-8933</idno><idno type="eISSN">1608-3245</idno><imprint><publisher>SP MAIK Nauka/Interperiodica</publisher><pubPlace>Dordrecht</pubPlace><date type="published" when="2013-01-01">2013-01-01</date><biblScope unit="volume">47</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="105">105</biblScope><biblScope unit="page" to="111">111</biblScope></imprint><idno type="ISSN">0026-8933</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0026-8933</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Pseudomonas</term><term>and scpA gene</term><term>caprolactam</term><term>nahU gene</term><term>plasmid</term><term>salicylate 1-hydroxylase</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Both caprolactams and salicylate biodegradation by Pseudomonas salicylate/caprolactam degraders are controlled by large conjugative plasmids (SAL/CAP). Some of these plasmids have been assigned to the P-7 incompatibility group. The new salicylate 1-hydroxylase gene (scpA) has been detected in SAL/CAP plasmids and partially sequenced. The scpA gene was equally related to the closest homolog genes nahG (NAH7), salA (P. reinekei MT1), and nahU (pND6-1); however, the identity rate did not exceed 72–74%. The synthesis of salicylate 1-hydroxylase ScpA was not induced by salicylate. This enzyme had wide substrate specificity and exhibited the highest specific activity toward 4-methylsalicylate and nonsubstituted salicylate substrates. Furthermore, conjugative pseudomonads’ plasmids of salicylate degradation without the classical nah2 operon, which harbors only salicylate 1-hydroxylase gene nahU have been described for the first time.</div></front></TEI><istex><corpusName>springer-journals</corpusName><author><json:item><name>A. V. Panov</name><affiliations><json:string>Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia</json:string><json:string>E-mail: panov_a_v@inbox.ru</json:string></affiliations></json:item><json:item><name>O. V. Volkova</name><affiliations><json:string>Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia</json:string></affiliations></json:item><json:item><name>I. F. Puntus</name><affiliations><json:string>Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia</json:string></affiliations></json:item><json:item><name>T. Z. Esikova</name><affiliations><json:string>Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia</json:string></affiliations></json:item><json:item><name>I. A. Kosheleva</name><affiliations><json:string>Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia</json:string><json:string>Pushchino State Educational Institute of Natural Sciences, 142290, Pushchino, Russia</json:string></affiliations></json:item><json:item><name>A. M. Boronin</name><affiliations><json:string>Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia</json:string><json:string>Pushchino State Educational Institute of Natural Sciences, 142290, Pushchino, Russia</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>salicylate 1-hydroxylase</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>caprolactam</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Pseudomonas</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>plasmid</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>nahU gene</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>and scpA gene</value></json:item></subject><articleId><json:string>6438</json:string><json:string>S0026893313010147</json:string></articleId><arkIstex>ark:/67375/VQC-B2N6BP0F-G</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Abstract: Both caprolactams and salicylate biodegradation by Pseudomonas salicylate/caprolactam degraders are controlled by large conjugative plasmids (SAL/CAP). Some of these plasmids have been assigned to the P-7 incompatibility group. The new salicylate 1-hydroxylase gene (scpA) has been detected in SAL/CAP plasmids and partially sequenced. The scpA gene was equally related to the closest homolog genes nahG (NAH7), salA (P. reinekei MT1), and nahU (pND6-1); however, the identity rate did not exceed 72–74%. The synthesis of salicylate 1-hydroxylase ScpA was not induced by salicylate. This enzyme had wide substrate specificity and exhibited the highest specific activity toward 4-methylsalicylate and nonsubstituted salicylate substrates. Furthermore, conjugative pseudomonads’ plasmids of salicylate degradation without the classical nah2 operon, which harbors only salicylate 1-hydroxylase gene nahU have been described for the first time.</abstract><qualityIndicators><score>7.933</score><pdfWordCount>4409</pdfWordCount><pdfCharCount>30021</pdfCharCount><pdfVersion>1.4</pdfVersion><pdfPageCount>7</pdfPageCount><pdfPageSize>612 x 792 pts (letter)</pdfPageSize><refBibsNative>false</refBibsNative><abstractWordCount>127</abstractWordCount><abstractCharCount>950</abstractCharCount><keywordCount>6</keywordCount></qualityIndicators><title>scpA , a new salicylate hydroxylase gene localized in salicylate/caprolactam degradation plasmids</title><genre><json:string>research-article</json:string></genre><host><title>Molecular Biology</title><language><json:string>unknown</json:string></language><publicationDate>2013</publicationDate><copyrightDate>2013</copyrightDate><issn><json:string>0026-8933</json:string></issn><eissn><json:string>1608-3245</json:string></eissn><journalId><json:string>11008</json:string></journalId><volume>47</volume><issue>1</issue><pages><first>105</first><last>111</last></pages><genre><json:string>journal</json:string></genre><subject><json:item><value>Biomedical and Life Sciences</value></json:item><json:item><value>Life Sciences</value></json:item><json:item><value>Life Sciences, general</value></json:item><json:item><value>Biochemistry, general</value></json:item><json:item><value>Human Genetics</value></json:item></subject></host><ark><json:string>ark:/67375/VQC-B2N6BP0F-G</json:string></ark><publicationDate>2013</publicationDate><copyrightDate>2013</copyrightDate><doi><json:string>10.1134/S0026893313010147</json:string></doi><id>E231555660B45BF3E940BABD7C18E771EA67268A</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-B2N6BP0F-G/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-B2N6BP0F-G/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/VQC-B2N6BP0F-G/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">scpA , a new salicylate hydroxylase gene localized in salicylate/caprolactam degradation plasmids</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">SP MAIK Nauka/Interperiodica</publisher><pubPlace>Dordrecht</pubPlace><availability><licence><p>Pleiades Publishing, Ltd., 2013</p></licence><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</p></availability><date>2012-06-25</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note>Genomics. Transcriptomics</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">scpA , a new salicylate hydroxylase gene localized in salicylate/caprolactam degradation plasmids</title><author xml:id="author-0000" corresp="yes"><persName><forename type="first">A.</forename><surname>Panov</surname></persName><email>panov_a_v@inbox.ru</email><affiliation>Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia</affiliation></author><author xml:id="author-0001"><persName><forename type="first">O.</forename><surname>Volkova</surname></persName><affiliation>Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia</affiliation></author><author xml:id="author-0002"><persName><forename type="first">I.</forename><surname>Puntus</surname></persName><affiliation>Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia</affiliation></author><author xml:id="author-0003"><persName><forename type="first">T.</forename><surname>Esikova</surname></persName><affiliation>Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia</affiliation></author><author xml:id="author-0004"><persName><forename type="first">I.</forename><surname>Kosheleva</surname></persName><affiliation>Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia</affiliation><affiliation>Pushchino State Educational Institute of Natural Sciences, 142290, Pushchino, Russia</affiliation></author><author xml:id="author-0005"><persName><forename type="first">A.</forename><surname>Boronin</surname></persName><affiliation>Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia</affiliation><affiliation>Pushchino State Educational Institute of Natural Sciences, 142290, Pushchino, Russia</affiliation></author><idno type="istex">E231555660B45BF3E940BABD7C18E771EA67268A</idno><idno type="ark">ark:/67375/VQC-B2N6BP0F-G</idno><idno type="DOI">10.1134/S0026893313010147</idno><idno type="article-id">6438</idno><idno type="article-id">S0026893313010147</idno></analytic><monogr><title level="j">Molecular Biology</title><title level="j" type="abbrev">Mol Biol</title><idno type="pISSN">0026-8933</idno><idno type="eISSN">1608-3245</idno><idno type="journal-ID">true</idno><idno type="issue-article-count">16</idno><idno type="volume-issue-count">6</idno><imprint><publisher>SP MAIK Nauka/Interperiodica</publisher><pubPlace>Dordrecht</pubPlace><date type="published" when="2013-01-01"></date><biblScope unit="volume">47</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="105">105</biblScope><biblScope unit="page" to="111">111</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2012-06-25</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Both caprolactams and salicylate biodegradation by Pseudomonas salicylate/caprolactam degraders are controlled by large conjugative plasmids (SAL/CAP). Some of these plasmids have been assigned to the P-7 incompatibility group. The new salicylate 1-hydroxylase gene (scpA) has been detected in SAL/CAP plasmids and partially sequenced. The scpA gene was equally related to the closest homolog genes nahG (NAH7), salA (P. reinekei MT1), and nahU (pND6-1); however, the identity rate did not exceed 72–74%. The synthesis of salicylate 1-hydroxylase ScpA was not induced by salicylate. This enzyme had wide substrate specificity and exhibited the highest specific activity toward 4-methylsalicylate and nonsubstituted salicylate substrates. Furthermore, conjugative pseudomonads’ plasmids of salicylate degradation without the classical nah2 operon, which harbors only salicylate 1-hydroxylase gene nahU have been described for the first time.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>salicylate 1-hydroxylase</term></item><item><term>caprolactam</term></item><item><term>Pseudomonas</term></item><item><term>plasmid</term></item><item><term>nahU gene</term></item><item><term>and scpA gene</term></item></list></keywords></textClass><textClass><keywords scheme="Journal-Subject-Collection"><list><label>SC3</label><item><term>Biomedical and Life Sciences</term></item></list></keywords></textClass><textClass><keywords scheme="Journal-Subject-Group"><list><label>SCL</label><item><term>Life Sciences</term></item><label>SCL00004</label><item><term>Life Sciences, general</term></item><label>SCL14005</label><item><term>Biochemistry, general</term></item><label>SCB12008</label><item><term>Human Genetics</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2012-06-25">Created</change><change when="2013-01-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-B2N6BP0F-G/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus springer-journals not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" name="istex:docType"></istex:docType><istex:document><Publisher><PublisherInfo><PublisherName>SP MAIK Nauka/Interperiodica</PublisherName><PublisherLocation>Dordrecht</PublisherLocation><PublisherImprintName>MAIK Nauka/Interperiodica</PublisherImprintName></PublisherInfo><Journal OutputMedium="All"><JournalInfo JournalProductType="ArchiveJournal" NumberingStyle="Unnumbered"><JournalID>11008</JournalID><JournalPrintISSN>0026-8933</JournalPrintISSN><JournalElectronicISSN>1608-3245</JournalElectronicISSN><JournalTitle>Molecular Biology</JournalTitle><JournalAbbreviatedTitle>Mol Biol</JournalAbbreviatedTitle><JournalSubjectGroup><JournalSubject Code="SCL" Type="Primary">Life Sciences</JournalSubject><JournalSubject Code="SCL00004" Priority="1" Type="Secondary">Life Sciences, general</JournalSubject><JournalSubject Code="SCL14005" Priority="2" Type="Secondary">Biochemistry, general</JournalSubject><JournalSubject Code="SCB12008" Priority="3" Type="Secondary">Human Genetics</JournalSubject><SubjectCollection Code="SC3">Biomedical and Life Sciences</SubjectCollection></JournalSubjectGroup></JournalInfo><Volume OutputMedium="All"><VolumeInfo TocLevels="0" VolumeType="Regular"><VolumeIDStart>47</VolumeIDStart><VolumeIDEnd>47</VolumeIDEnd><VolumeIssueCount>6</VolumeIssueCount></VolumeInfo><Issue IssueType="Regular" OutputMedium="All"><IssueInfo IssueType="Regular" TocLevels="0"><IssueIDStart>1</IssueIDStart><IssueIDEnd>1</IssueIDEnd><IssueArticleCount>16</IssueArticleCount><IssueHistory><OnlineDate><Year>2013</Year><Month>2</Month><Day>21</Day></OnlineDate><CoverDate><Year>2013</Year><Month>1</Month></CoverDate><PricelistYear>2013</PricelistYear></IssueHistory><IssueCopyright><CopyrightHolderName>Pleiades Publishing, Ltd.</CopyrightHolderName><CopyrightYear>2013</CopyrightYear></IssueCopyright></IssueInfo><Article ID="S0026893313010147" OutputMedium="All"><ArticleInfo ArticleType="OriginalPaper" ContainsESM="No" Language="En" NumberingStyle="Unnumbered" TocLevels="0"><ArticleID>6438</ArticleID><ArticleDOI>10.1134/S0026893313010147</ArticleDOI><ArticleSequenceNumber>9</ArticleSequenceNumber><ArticleTitle Language="En"><Emphasis Type="Italic">scpA</Emphasis>, a new salicylate hydroxylase gene localized in salicylate/caprolactam degradation plasmids</ArticleTitle><ArticleCategory>Genomics. Transcriptomics</ArticleCategory><ArticleFirstPage>105</ArticleFirstPage><ArticleLastPage>111</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2013</Year><Month>2</Month><Day>20</Day></RegistrationDate><Received><Year>2012</Year><Month>6</Month><Day>25</Day></Received><Accepted><Year>2012</Year><Month>7</Month><Day>5</Day></Accepted><OnlineDate><Year>2013</Year><Month>2</Month><Day>21</Day></OnlineDate></ArticleHistory><ArticleCopyright><CopyrightHolderName>Pleiades Publishing, Ltd.</CopyrightHolderName><CopyrightYear>2013</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>A.</GivenName><GivenName>V.</GivenName><FamilyName>Panov</FamilyName></AuthorName><Contact><Email>panov_a_v@inbox.ru</Email></Contact></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>O.</GivenName><GivenName>V.</GivenName><FamilyName>Volkova</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>I.</GivenName><GivenName>F.</GivenName><FamilyName>Puntus</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>T.</GivenName><GivenName>Z.</GivenName><FamilyName>Esikova</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff2"><AuthorName DisplayOrder="Western"><GivenName>I.</GivenName><GivenName>A.</GivenName><FamilyName>Kosheleva</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff2"><AuthorName DisplayOrder="Western"><GivenName>A.</GivenName><GivenName>M.</GivenName><FamilyName>Boronin</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgDivision>Skryabin Institute of Biochemistry and Physiology of Microorganisms</OrgDivision><OrgName>Russian Academy of Sciences</OrgName><OrgAddress><City>Pushchino</City><Postcode>142290</Postcode><Country Code="RU">Russia</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgName>Pushchino State Educational Institute of Natural Sciences</OrgName><OrgAddress><City>Pushchino</City><Postcode>142290</Postcode><Country Code="RU">Russia</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading><Para>Both caprolactams and salicylate biodegradation by <Emphasis Type="Italic">Pseudomonas</Emphasis> salicylate/caprolactam degraders are controlled by large conjugative plasmids (SAL/CAP). Some of these plasmids have been assigned to the P-7 incompatibility group. The new salicylate 1-hydroxylase gene (<Emphasis Type="Italic">scpA</Emphasis>) has been detected in SAL/CAP plasmids and partially sequenced. The <Emphasis Type="Italic">scpA</Emphasis> gene was equally related to the closest homolog genes <Emphasis Type="Italic">nahG</Emphasis> (NAH7), <Emphasis Type="Italic">salA</Emphasis> (<Emphasis Type="Italic">P. reinekei</Emphasis> MT1), and <Emphasis Type="Italic">nahU</Emphasis> (pND6-1); however, the identity rate did not exceed 72–74%. The synthesis of salicylate 1-hydroxylase ScpA was not induced by salicylate. This enzyme had wide substrate specificity and exhibited the highest specific activity toward 4-methylsalicylate and nonsubstituted salicylate substrates. Furthermore, conjugative pseudomonads’ plasmids of salicylate degradation without the classical <Emphasis Type="Italic">nah2</Emphasis> operon, which harbors only salicylate 1-hydroxylase gene <Emphasis Type="Italic">nahU</Emphasis> have been described for the first time.</Para></Abstract><KeywordGroup Language="En"><Heading>Keywords</Heading><Keyword>salicylate 1-hydroxylase</Keyword><Keyword>caprolactam</Keyword><Keyword><Emphasis Type="Italic">Pseudomonas</Emphasis></Keyword><Keyword>plasmid</Keyword><Keyword><Emphasis Type="Italic">nahU</Emphasis> gene</Keyword><Keyword>and <Emphasis Type="Italic">scpA</Emphasis> gene</Keyword></KeywordGroup><ArticleNote Type="Misc"><SimplePara>Original Russian Text © A.V. Panov, O.V. Volkova, I.F. Puntus, T.Z. Esikova, I.A. Kosheleva, A.M. Boronin, 2013, published in Molekulyarnaya Biologiya, 2013, Vol. 47, No. 1, pp. 116–123.</SimplePara></ArticleNote></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>scpA , a new salicylate hydroxylase gene localized in salicylate/caprolactam degradation plasmids</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title> scpA , a new salicylate hydroxylase gene localized in salicylate/caprolactam degradation plasmids</title></titleInfo><name type="personal" displayLabel="corresp"><namePart type="given">A.</namePart><namePart type="given">V.</namePart><namePart type="family">Panov</namePart><affiliation>Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia</affiliation><affiliation>E-mail: panov_a_v@inbox.ru</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">O.</namePart><namePart type="given">V.</namePart><namePart type="family">Volkova</namePart><affiliation>Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">I.</namePart><namePart type="given">F.</namePart><namePart type="family">Puntus</namePart><affiliation>Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">T.</namePart><namePart type="given">Z.</namePart><namePart type="family">Esikova</namePart><affiliation>Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">I.</namePart><namePart type="given">A.</namePart><namePart type="family">Kosheleva</namePart><affiliation>Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia</affiliation><affiliation>Pushchino State Educational Institute of Natural Sciences, 142290, Pushchino, Russia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="given">M.</namePart><namePart type="family">Boronin</namePart><affiliation>Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Russia</affiliation><affiliation>Pushchino State Educational Institute of Natural Sciences, 142290, Pushchino, Russia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>SP MAIK Nauka/Interperiodica</publisher><place><placeTerm type="text">Dordrecht</placeTerm></place><dateCreated encoding="w3cdtf">2012-06-25</dateCreated><dateIssued encoding="w3cdtf">2013-01-01</dateIssued><copyrightDate encoding="w3cdtf">2013</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: Both caprolactams and salicylate biodegradation by Pseudomonas salicylate/caprolactam degraders are controlled by large conjugative plasmids (SAL/CAP). Some of these plasmids have been assigned to the P-7 incompatibility group. The new salicylate 1-hydroxylase gene (scpA) has been detected in SAL/CAP plasmids and partially sequenced. The scpA gene was equally related to the closest homolog genes nahG (NAH7), salA (P. reinekei MT1), and nahU (pND6-1); however, the identity rate did not exceed 72–74%. The synthesis of salicylate 1-hydroxylase ScpA was not induced by salicylate. This enzyme had wide substrate specificity and exhibited the highest specific activity toward 4-methylsalicylate and nonsubstituted salicylate substrates. Furthermore, conjugative pseudomonads’ plasmids of salicylate degradation without the classical nah2 operon, which harbors only salicylate 1-hydroxylase gene nahU have been described for the first time.</abstract><note>Genomics. Transcriptomics</note><subject lang="en"><genre>Keywords</genre><topic>salicylate 1-hydroxylase</topic><topic>caprolactam</topic><topic>Pseudomonas</topic><topic>plasmid</topic><topic>nahU gene</topic><topic>and scpA gene</topic></subject><relatedItem type="host"><titleInfo><title>Molecular Biology</title></titleInfo><titleInfo type="abbreviated"><title>Mol Biol</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">2013-02-21</dateIssued><copyrightDate encoding="w3cdtf">2013</copyrightDate></originInfo><subject><genre>Journal-Subject-Collection</genre><topic authority="SpringerSubjectCodes" authorityURI="SC3">Biomedical and Life Sciences</topic></subject><subject><genre>Journal-Subject-Group</genre><topic authority="SpringerSubjectCodes" authorityURI="SCL">Life Sciences</topic><topic authority="SpringerSubjectCodes" authorityURI="SCL00004">Life Sciences, general</topic><topic authority="SpringerSubjectCodes" authorityURI="SCL14005">Biochemistry, general</topic><topic authority="SpringerSubjectCodes" authorityURI="SCB12008">Human Genetics</topic></subject><identifier type="ISSN">0026-8933</identifier><identifier type="eISSN">1608-3245</identifier><identifier type="JournalID">11008</identifier><identifier type="IssueArticleCount">16</identifier><identifier type="VolumeIssueCount">6</identifier><part><date>2013</date><detail type="volume"><number>47</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="pages"><start>105</start><end>111</end></extent></part><recordInfo><recordOrigin>Pleiades Publishing, Ltd., 2013</recordOrigin></recordInfo></relatedItem><identifier type="istex">E231555660B45BF3E940BABD7C18E771EA67268A</identifier><identifier type="ark">ark:/67375/VQC-B2N6BP0F-G</identifier><identifier type="DOI">10.1134/S0026893313010147</identifier><identifier type="ArticleID">6438</identifier><identifier type="ArticleID">S0026893313010147</identifier><accessCondition type="use and reproduction" contentType="copyright">Pleiades Publishing, Ltd., 2013</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Pleiades Publishing, Ltd., 2013</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-B2N6BP0F-G/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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