The LuxR family members GdmRI and GdmRII are positive regulators of geldanamycin biosynthesis in Streptomyces hygroscopicus 17997
Identifieur interne : 001101 ( Istex/Corpus ); précédent : 001100; suivant : 001102The LuxR family members GdmRI and GdmRII are positive regulators of geldanamycin biosynthesis in Streptomyces hygroscopicus 17997
Auteurs : Weiqing He ; Jian Lei ; Yuying Liu ; Yiguang WangSource :
- Archives of Microbiology [ 0302-8933 ] ; 2008-05-01.
English descriptors
Abstract
Abstract: The recent sequencing of the DNA region of the geldanamycin post-polyketide synthase (PKS) modification gene clusters revealed the presence of two regulatory genes: gdmRI (2,907 bp) and gdmRII (2,766 bp). The deduced products of gdmRI and gdmRII (968 and 921 amino acid residues, respectively) were identified as homologues of the LuxR transcriptional regulatory proteins. Inactivation by gene replacement of gdmRI or gdmRII in the Streptomyces hygroscopicus 17997 genome resulted in a complete loss of geldanamycin production. Complementation by a plasmid carrying gdmRI or gdmRII restored geldanamycin production, suggesting that the products of these two regulatory genes are positive regulators that are required for geldanamycin biosynthesis. The gdmRI transcript was detected in the ΔgdmRII mutant, and the gdmRII was detected in the ΔgdmRI mutant, indicating that the two genes are transcribed independently and do not regulate each other. Time course of gene expression analysis by RT-PCR of the geldanamycin biosynthetic genes showed that the transcription of gdmRI and gdmRII correlates with that of genes involved in polyketide biosynthesis, but not with the post-PKS modification gene gdmN, whose transcription is initiated earlier. gdmRI or gdmRII gene disruptants did not transcribe the polyketide biosynthetic related genes pks, gdmF, and gdnA-O-P, but did trancribe gdmN. These results demonstrated that gdmRI and gdmRII are pathway-specific positive regulators that control the polyketide biosynthetic genes in geldanamycin biosynthsis, but not the post-PKS modification gene, gdmN.
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DOI: 10.1007/s00203-007-0346-2
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China</mods:affiliation></affiliation></author><author><name sortKey="Liu, Yuying" sort="Liu, Yuying" uniqKey="Liu Y" first="Yuying" last="Liu">Yuying Liu</name><affiliation><mods:affiliation>Key Laboratory of Biotechnology of Antibiotics, Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, 100050, Beijing, China</mods:affiliation></affiliation></author><author><name sortKey="Wang, Yiguang" sort="Wang, Yiguang" uniqKey="Wang Y" first="Yiguang" last="Wang">Yiguang Wang</name><affiliation><mods:affiliation>Key Laboratory of Biotechnology of Antibiotics, Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, 100050, Beijing, China</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: wangyh456@yahoo.com.cn</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno 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China</mods:affiliation></affiliation></author><author><name sortKey="Lei, Jian" sort="Lei, Jian" uniqKey="Lei J" first="Jian" last="Lei">Jian Lei</name><affiliation><mods:affiliation>Key Laboratory of Biotechnology of Antibiotics, Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, 100050, Beijing, China</mods:affiliation></affiliation></author><author><name sortKey="Liu, Yuying" sort="Liu, Yuying" uniqKey="Liu Y" first="Yuying" last="Liu">Yuying Liu</name><affiliation><mods:affiliation>Key Laboratory of Biotechnology of Antibiotics, Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, 100050, Beijing, China</mods:affiliation></affiliation></author><author><name sortKey="Wang, Yiguang" sort="Wang, Yiguang" uniqKey="Wang Y" first="Yiguang" last="Wang">Yiguang Wang</name><affiliation><mods:affiliation>Key Laboratory of Biotechnology of Antibiotics, Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, 100050, Beijing, China</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: wangyh456@yahoo.com.cn</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Archives of Microbiology</title><title level="j" type="abbrev">Arch Microbiol</title><idno type="ISSN">0302-8933</idno><idno type="eISSN">1432-072X</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="2008-05-01">2008-05-01</date><biblScope unit="volume">189</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="501">501</biblScope><biblScope unit="page" to="510">510</biblScope></imprint><idno type="ISSN">0302-8933</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0302-8933</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Geldanamycin biosynthesis</term><term>Lux R family members</term><term>Positive regulator</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The recent sequencing of the DNA region of the geldanamycin post-polyketide synthase (PKS) modification gene clusters revealed the presence of two regulatory genes: gdmRI (2,907 bp) and gdmRII (2,766 bp). The deduced products of gdmRI and gdmRII (968 and 921 amino acid residues, respectively) were identified as homologues of the LuxR transcriptional regulatory proteins. Inactivation by gene replacement of gdmRI or gdmRII in the Streptomyces hygroscopicus 17997 genome resulted in a complete loss of geldanamycin production. Complementation by a plasmid carrying gdmRI or gdmRII restored geldanamycin production, suggesting that the products of these two regulatory genes are positive regulators that are required for geldanamycin biosynthesis. The gdmRI transcript was detected in the ΔgdmRII mutant, and the gdmRII was detected in the ΔgdmRI mutant, indicating that the two genes are transcribed independently and do not regulate each other. Time course of gene expression analysis by RT-PCR of the geldanamycin biosynthetic genes showed that the transcription of gdmRI and gdmRII correlates with that of genes involved in polyketide biosynthesis, but not with the post-PKS modification gene gdmN, whose transcription is initiated earlier. gdmRI or gdmRII gene disruptants did not transcribe the polyketide biosynthetic related genes pks, gdmF, and gdnA-O-P, but did trancribe gdmN. These results demonstrated that gdmRI and gdmRII are pathway-specific positive regulators that control the polyketide biosynthetic genes in geldanamycin biosynthsis, but not the post-PKS modification gene, gdmN.</div></front></TEI><istex><corpusName>springer-journals</corpusName><author><json:item><name>Weiqing He</name><affiliations><json:string>Key Laboratory of Biotechnology of Antibiotics, Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, 100050, Beijing, China</json:string></affiliations></json:item><json:item><name>Jian Lei</name><affiliations><json:string>Key Laboratory of Biotechnology of Antibiotics, Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, 100050, Beijing, China</json:string></affiliations></json:item><json:item><name>Yuying Liu</name><affiliations><json:string>Key Laboratory of Biotechnology of Antibiotics, Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, 100050, Beijing, China</json:string></affiliations></json:item><json:item><name>Yiguang Wang</name><affiliations><json:string>Key Laboratory of Biotechnology of Antibiotics, Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, 100050, Beijing, China</json:string><json:string>E-mail: wangyh456@yahoo.com.cn</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Geldanamycin biosynthesis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Positive regulator</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Lux R family members</value></json:item></subject><articleId><json:string>346</json:string><json:string>s00203-007-0346-2</json:string></articleId><arkIstex>ark:/67375/VQC-760C7K7F-W</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Abstract: The recent sequencing of the DNA region of the geldanamycin post-polyketide synthase (PKS) modification gene clusters revealed the presence of two regulatory genes: gdmRI (2,907 bp) and gdmRII (2,766 bp). The deduced products of gdmRI and gdmRII (968 and 921 amino acid residues, respectively) were identified as homologues of the LuxR transcriptional regulatory proteins. Inactivation by gene replacement of gdmRI or gdmRII in the Streptomyces hygroscopicus 17997 genome resulted in a complete loss of geldanamycin production. Complementation by a plasmid carrying gdmRI or gdmRII restored geldanamycin production, suggesting that the products of these two regulatory genes are positive regulators that are required for geldanamycin biosynthesis. The gdmRI transcript was detected in the ΔgdmRII mutant, and the gdmRII was detected in the ΔgdmRI mutant, indicating that the two genes are transcribed independently and do not regulate each other. Time course of gene expression analysis by RT-PCR of the geldanamycin biosynthetic genes showed that the transcription of gdmRI and gdmRII correlates with that of genes involved in polyketide biosynthesis, but not with the post-PKS modification gene gdmN, whose transcription is initiated earlier. gdmRI or gdmRII gene disruptants did not transcribe the polyketide biosynthetic related genes pks, gdmF, and gdnA-O-P, but did trancribe gdmN. 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xml:id="author-0003" corresp="yes"><persName><forename type="first">Yiguang</forename><surname>Wang</surname></persName><email>wangyh456@yahoo.com.cn</email><affiliation>Key Laboratory of Biotechnology of Antibiotics, Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, 100050, Beijing, China</affiliation></author><idno type="istex">F7752652196076C6A08343BF978E783F01412A8F</idno><idno type="ark">ark:/67375/VQC-760C7K7F-W</idno><idno type="DOI">10.1007/s00203-007-0346-2</idno><idno type="article-id">346</idno><idno type="article-id">s00203-007-0346-2</idno></analytic><monogr><title level="j">Archives of Microbiology</title><title level="j" type="abbrev">Arch Microbiol</title><idno type="pISSN">0302-8933</idno><idno type="eISSN">1432-072X</idno><idno type="journal-ID">true</idno><idno type="issue-article-count">11</idno><idno 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The deduced products of gdmRI and gdmRII (968 and 921 amino acid residues, respectively) were identified as homologues of the LuxR transcriptional regulatory proteins. Inactivation by gene replacement of gdmRI or gdmRII in the Streptomyces hygroscopicus 17997 genome resulted in a complete loss of geldanamycin production. Complementation by a plasmid carrying gdmRI or gdmRII restored geldanamycin production, suggesting that the products of these two regulatory genes are positive regulators that are required for geldanamycin biosynthesis. The gdmRI transcript was detected in the ΔgdmRII mutant, and the gdmRII was detected in the ΔgdmRI mutant, indicating that the two genes are transcribed independently and do not regulate each other. Time course of gene expression analysis by RT-PCR of the geldanamycin biosynthetic genes showed that the transcription of gdmRI and gdmRII correlates with that of genes involved in polyketide biosynthesis, but not with the post-PKS modification gene gdmN, whose transcription is initiated earlier. gdmRI or gdmRII gene disruptants did not transcribe the polyketide biosynthetic related genes pks, gdmF, and gdnA-O-P, but did trancribe gdmN. These results demonstrated that gdmRI and gdmRII are pathway-specific positive regulators that control the polyketide biosynthetic genes in geldanamycin biosynthsis, but not the post-PKS modification gene, gdmN.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Geldanamycin biosynthesis</term></item><item><term>Positive regulator</term></item><item><term>Lux R family members</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>Life Sciences</head><item><term>Ecology</term></item><item><term>Biotechnology</term></item><item><term>Cell Biology</term></item><item><term>Biochemistry, general</term></item><item><term>Microbial Ecology</term></item><item><term>Microbiology</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2007-03-18">Created</change><change 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hygroscopicus</Emphasis> 17997</ArticleTitle><ArticleCategory>Original Paper</ArticleCategory><ArticleFirstPage>501</ArticleFirstPage><ArticleLastPage>510</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2007</Year><Month>12</Month><Day>18</Day></RegistrationDate><Received><Year>2007</Year><Month>3</Month><Day>18</Day></Received><Revised><Year>2007</Year><Month>10</Month><Day>17</Day></Revised><Accepted><Year>2007</Year><Month>12</Month><Day>11</Day></Accepted><OnlineDate><Year>2008</Year><Month>1</Month><Day>24</Day></OnlineDate></ArticleHistory><ArticleEditorialResponsibility>Jean-Luc Pernodet</ArticleEditorialResponsibility><ArticleCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>2008</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Weiqing</GivenName><FamilyName>He</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Jian</GivenName><FamilyName>Lei</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Yuying</GivenName><FamilyName>Liu</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Yiguang</GivenName><FamilyName>Wang</FamilyName></AuthorName><Contact><Phone>+86-10-63038137</Phone><Fax>+86-10-63176489</Fax><Email>wangyh456@yahoo.com.cn</Email></Contact></Author><Affiliation ID="Aff1"><OrgDivision>Key Laboratory of Biotechnology of Antibiotics, Ministry of Health, Institute of Medicinal Biotechnology</OrgDivision><OrgName>Chinese Academy of Medical Sciences, Peking Union Medical College</OrgName><OrgAddress><City>Beijing</City><Postcode>100050</Postcode><Country Code="CN">China</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En" OutputMedium="All"><Heading>Abstract</Heading><Para>The recent sequencing of the DNA region of the geldanamycin post-polyketide synthase (PKS) modification gene clusters revealed the presence of two regulatory genes: <Emphasis Type="Italic">gdmRI</Emphasis> (2,907 bp) and <Emphasis Type="Italic">gdmRII</Emphasis> (2,766 bp). The deduced products of <Emphasis Type="Italic">gdmRI</Emphasis> and <Emphasis Type="Italic">gdmRII</Emphasis> (968 and 921 amino acid residues, respectively) were identified as homologues of the LuxR transcriptional regulatory proteins. Inactivation by gene replacement of <Emphasis Type="Italic">gdmRI</Emphasis> or <Emphasis Type="Italic">gdmRII</Emphasis> in the <Emphasis Type="Italic">Streptomyces hygroscopicus</Emphasis> 17997 genome resulted in a complete loss of geldanamycin production. Complementation by a plasmid carrying <Emphasis Type="Italic">gdmRI</Emphasis> or <Emphasis Type="Italic">gdmRII</Emphasis> restored geldanamycin production, suggesting that the products of these two regulatory genes are positive regulators that are required for geldanamycin biosynthesis. The <Emphasis Type="Italic">gdmRI</Emphasis> transcript was detected in the Δ<Emphasis Type="Italic">gdmRII</Emphasis> mutant, and the <Emphasis Type="Italic">gdm</Emphasis>RII was detected in the Δ<Emphasis Type="Italic">gdmRI</Emphasis> mutant, indicating that the two genes are transcribed independently and do not regulate each other. Time course of gene expression analysis by RT-PCR of the geldanamycin biosynthetic genes showed that the transcription of <Emphasis Type="Italic">gdmRI</Emphasis> and <Emphasis Type="Italic">gdmRII</Emphasis> correlates with that of genes involved in polyketide biosynthesis, but not with the post-PKS modification gene <Emphasis Type="Italic">gdmN</Emphasis>, whose transcription is initiated earlier. <Emphasis Type="Italic">gdmRI</Emphasis> or <Emphasis Type="Italic">gdmRII</Emphasis> gene disruptants did not transcribe the polyketide biosynthetic related genes <Emphasis Type="Italic">pks, gdmF</Emphasis>, and <Emphasis Type="Italic">gdnA-O-P</Emphasis>, but did trancribe <Emphasis Type="Italic">gdmN</Emphasis>. These results demonstrated that <Emphasis Type="Italic">gdmRI</Emphasis> and <Emphasis Type="Italic">gdmRII</Emphasis> are pathway-specific positive regulators that control the polyketide biosynthetic genes in geldanamycin biosynthsis, but not the post-PKS modification gene, <Emphasis Type="Italic">gdmN.</Emphasis></Para></Abstract><KeywordGroup Language="En" OutputMedium="All"><Heading>Keywords</Heading><Keyword>Geldanamycin biosynthesis</Keyword><Keyword>Positive regulator</Keyword><Keyword>Lux R family members</Keyword></KeywordGroup><ArticleNote Type="CommunicatedBy"><SimplePara>Communicated by Jean-Luc Pernodet.</SimplePara></ArticleNote></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>The LuxR family members GdmRI and GdmRII are positive regulators of geldanamycin biosynthesis in Streptomyces hygroscopicus 17997</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>The LuxR family members GdmRI and GdmRII are positive regulators of geldanamycin biosynthesis in Streptomyces hygroscopicus 17997</title></titleInfo><name type="personal"><namePart type="given">Weiqing</namePart><namePart type="family">He</namePart><affiliation>Key Laboratory of Biotechnology of Antibiotics, Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, 100050, Beijing, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jian</namePart><namePart type="family">Lei</namePart><affiliation>Key Laboratory of Biotechnology of Antibiotics, Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, 100050, Beijing, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yuying</namePart><namePart type="family">Liu</namePart><affiliation>Key Laboratory of Biotechnology of Antibiotics, Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, 100050, Beijing, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Yiguang</namePart><namePart type="family">Wang</namePart><affiliation>Key Laboratory of Biotechnology of Antibiotics, Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, 100050, Beijing, China</affiliation><affiliation>E-mail: wangyh456@yahoo.com.cn</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer-Verlag</publisher><place><placeTerm type="text">Berlin/Heidelberg</placeTerm></place><dateCreated encoding="w3cdtf">2007-03-18</dateCreated><dateIssued encoding="w3cdtf">2008-05-01</dateIssued><copyrightDate encoding="w3cdtf">2008</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: The recent sequencing of the DNA region of the geldanamycin post-polyketide synthase (PKS) modification gene clusters revealed the presence of two regulatory genes: gdmRI (2,907 bp) and gdmRII (2,766 bp). The deduced products of gdmRI and gdmRII (968 and 921 amino acid residues, respectively) were identified as homologues of the LuxR transcriptional regulatory proteins. Inactivation by gene replacement of gdmRI or gdmRII in the Streptomyces hygroscopicus 17997 genome resulted in a complete loss of geldanamycin production. Complementation by a plasmid carrying gdmRI or gdmRII restored geldanamycin production, suggesting that the products of these two regulatory genes are positive regulators that are required for geldanamycin biosynthesis. The gdmRI transcript was detected in the ΔgdmRII mutant, and the gdmRII was detected in the ΔgdmRI mutant, indicating that the two genes are transcribed independently and do not regulate each other. Time course of gene expression analysis by RT-PCR of the geldanamycin biosynthetic genes showed that the transcription of gdmRI and gdmRII correlates with that of genes involved in polyketide biosynthesis, but not with the post-PKS modification gene gdmN, whose transcription is initiated earlier. gdmRI or gdmRII gene disruptants did not transcribe the polyketide biosynthetic related genes pks, gdmF, and gdnA-O-P, but did trancribe gdmN. These results demonstrated that gdmRI and gdmRII are pathway-specific positive regulators that control the polyketide biosynthetic genes in geldanamycin biosynthsis, but not the post-PKS modification gene, gdmN.</abstract><note>Original Paper</note><subject lang="en"><genre>Keywords</genre><topic>Geldanamycin biosynthesis</topic><topic>Positive regulator</topic><topic>Lux R family members</topic></subject><relatedItem type="host"><titleInfo><title>Archives of Microbiology</title></titleInfo><titleInfo type="abbreviated"><title>Arch Microbiol</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">2008-04-17</dateIssued><copyrightDate encoding="w3cdtf">2008</copyrightDate></originInfo><subject><genre>Life Sciences</genre><topic>Ecology</topic><topic>Biotechnology</topic><topic>Cell Biology</topic><topic>Biochemistry, general</topic><topic>Microbial Ecology</topic><topic>Microbiology</topic></subject><identifier type="ISSN">0302-8933</identifier><identifier type="eISSN">1432-072X</identifier><identifier type="JournalID">203</identifier><identifier type="IssueArticleCount">11</identifier><identifier type="VolumeIssueCount">6</identifier><part><date>2008</date><detail type="volume"><number>189</number><caption>vol.</caption></detail><detail type="issue"><number>5</number><caption>no.</caption></detail><extent unit="pages"><start>501</start><end>510</end></extent></part><recordInfo><recordOrigin>Springer-Verlag, 2008</recordOrigin></recordInfo></relatedItem><identifier type="istex">F7752652196076C6A08343BF978E783F01412A8F</identifier><identifier type="ark">ark:/67375/VQC-760C7K7F-W</identifier><identifier type="DOI">10.1007/s00203-007-0346-2</identifier><identifier type="ArticleID">346</identifier><identifier type="ArticleID">s00203-007-0346-2</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer-Verlag, 2008</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Springer-Verlag, 2008</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-760C7K7F-W/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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