DNA Oligonucleotides and Plasmids Perform Equally as Donors for Targeted Gene Conversion
Identifieur interne : 000C11 ( Istex/Corpus ); précédent : 000C10; suivant : 000C12DNA Oligonucleotides and Plasmids Perform Equally as Donors for Targeted Gene Conversion
Auteurs : Stefan Wagner ; Judi Mccracken ; Sally Cole ; Götz LaibleSource :
- Biochemical Genetics [ 0006-2928 ] ; 2010-12-01.
English descriptors
Abstract
Abstract: Site-specific gene modifications in cells are initiated by the introduction of exogenous DNA. We used a recently established cell assay to compare the ability of DNA donors to induce a single point mutation that converts a target gene encoding blue fluorescent protein (BFP) into expressing green fluorescent protein (GFP). In a chromosomal assay with cells stably expressing BFP, we showed that fluorescently labeled single-stranded oligonucleotides and a donor plasmid cotranscribing a red fluorescent protein provide similar efficiencies in triggering BFP–GFP conversions. In transient cotransfections, an isogenic donor plasmid comprising a nonfunctional GFP gene yielded a greater efficiency for the conversion of the BFP target gene than a nonisogenic donor, and all plasmid donors were superior to oligonucleotides.
Url:
DOI: 10.1007/s10528-010-9370-z
Links to Exploration step
ISTEX:4199191EDDA4C1C8FE9921F6EB4A0BED938224E2Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">DNA Oligonucleotides and Plasmids Perform Equally as Donors for Targeted Gene Conversion</title><author><name sortKey="Wagner, Stefan" sort="Wagner, Stefan" uniqKey="Wagner S" first="Stefan" last="Wagner">Stefan Wagner</name><affiliation><mods:affiliation>Reproductive Technologies, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton, New Zealand</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: stefan.wagner@agresearch.co.nz</mods:affiliation></affiliation></author><author><name sortKey="Mccracken, Judi" sort="Mccracken, Judi" uniqKey="Mccracken J" first="Judi" last="Mccracken">Judi Mccracken</name><affiliation><mods:affiliation>Reproductive Technologies, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton, New Zealand</mods:affiliation></affiliation></author><author><name sortKey="Cole, Sally" sort="Cole, Sally" uniqKey="Cole S" first="Sally" last="Cole">Sally Cole</name><affiliation><mods:affiliation>Reproductive Technologies, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton, New Zealand</mods:affiliation></affiliation></author><author><name sortKey="Laible, Gotz" sort="Laible, Gotz" uniqKey="Laible G" first="Götz" last="Laible">Götz Laible</name><affiliation><mods:affiliation>Reproductive Technologies, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton, New Zealand</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:4199191EDDA4C1C8FE9921F6EB4A0BED938224E2</idno><date when="2010" year="2010">2010</date><idno type="doi">10.1007/s10528-010-9370-z</idno><idno type="url">https://api.istex.fr/ark:/67375/VQC-X6FX9VZK-Z/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000C11</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000C11</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">DNA Oligonucleotides and Plasmids Perform Equally as Donors for Targeted Gene Conversion</title><author><name sortKey="Wagner, Stefan" sort="Wagner, Stefan" uniqKey="Wagner S" first="Stefan" last="Wagner">Stefan Wagner</name><affiliation><mods:affiliation>Reproductive Technologies, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton, New Zealand</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: stefan.wagner@agresearch.co.nz</mods:affiliation></affiliation></author><author><name sortKey="Mccracken, Judi" sort="Mccracken, Judi" uniqKey="Mccracken J" first="Judi" last="Mccracken">Judi Mccracken</name><affiliation><mods:affiliation>Reproductive Technologies, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton, New Zealand</mods:affiliation></affiliation></author><author><name sortKey="Cole, Sally" sort="Cole, Sally" uniqKey="Cole S" first="Sally" last="Cole">Sally Cole</name><affiliation><mods:affiliation>Reproductive Technologies, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton, New Zealand</mods:affiliation></affiliation></author><author><name sortKey="Laible, Gotz" sort="Laible, Gotz" uniqKey="Laible G" first="Götz" last="Laible">Götz Laible</name><affiliation><mods:affiliation>Reproductive Technologies, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton, New Zealand</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Biochemical Genetics</title><title level="j" type="abbrev">Biochem Genet</title><idno type="ISSN">0006-2928</idno><idno type="eISSN">1573-4927</idno><imprint><publisher>Springer US; http://www.springer-ny.com</publisher><pubPlace>Boston</pubPlace><date type="published" when="2010-12-01">2010-12-01</date><biblScope unit="volume">48</biblScope><biblScope unit="issue">11-12</biblScope><biblScope unit="page" from="897">897</biblScope><biblScope unit="page" to="908">908</biblScope></imprint><idno type="ISSN">0006-2928</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0006-2928</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Gene conversion</term><term>Green fluorescent protein</term><term>Oligonucleotide</term><term>Plasmid</term><term>Transfection</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Site-specific gene modifications in cells are initiated by the introduction of exogenous DNA. We used a recently established cell assay to compare the ability of DNA donors to induce a single point mutation that converts a target gene encoding blue fluorescent protein (BFP) into expressing green fluorescent protein (GFP). In a chromosomal assay with cells stably expressing BFP, we showed that fluorescently labeled single-stranded oligonucleotides and a donor plasmid cotranscribing a red fluorescent protein provide similar efficiencies in triggering BFP–GFP conversions. In transient cotransfections, an isogenic donor plasmid comprising a nonfunctional GFP gene yielded a greater efficiency for the conversion of the BFP target gene than a nonisogenic donor, and all plasmid donors were superior to oligonucleotides.</div></front></TEI><istex><corpusName>springer-journals</corpusName><author><json:item><name>Stefan Wagner</name><affiliations><json:string>Reproductive Technologies, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton, New Zealand</json:string><json:string>E-mail: stefan.wagner@agresearch.co.nz</json:string></affiliations></json:item><json:item><name>Judi McCracken</name><affiliations><json:string>Reproductive Technologies, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton, New Zealand</json:string></affiliations></json:item><json:item><name>Sally Cole</name><affiliations><json:string>Reproductive Technologies, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton, New Zealand</json:string></affiliations></json:item><json:item><name>Götz Laible</name><affiliations><json:string>Reproductive Technologies, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton, New Zealand</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Gene conversion</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Green fluorescent protein</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Plasmid</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Oligonucleotide</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Transfection</value></json:item></subject><articleId><json:string>9370</json:string><json:string>s10528-010-9370-z</json:string></articleId><arkIstex>ark:/67375/VQC-X6FX9VZK-Z</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Abstract: Site-specific gene modifications in cells are initiated by the introduction of exogenous DNA. We used a recently established cell assay to compare the ability of DNA donors to induce a single point mutation that converts a target gene encoding blue fluorescent protein (BFP) into expressing green fluorescent protein (GFP). In a chromosomal assay with cells stably expressing BFP, we showed that fluorescently labeled single-stranded oligonucleotides and a donor plasmid cotranscribing a red fluorescent protein provide similar efficiencies in triggering BFP–GFP conversions. In transient cotransfections, an isogenic donor plasmid comprising a nonfunctional GFP gene yielded a greater efficiency for the conversion of the BFP target gene than a nonisogenic donor, and all plasmid donors were superior to oligonucleotides.</abstract><qualityIndicators><refBibsNative>false</refBibsNative><abstractWordCount>118</abstractWordCount><abstractCharCount>832</abstractCharCount><keywordCount>5</keywordCount><score>7.613</score><pdfWordCount>4197</pdfWordCount><pdfCharCount>26723</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>12</pdfPageCount><pdfPageSize>439.37 x 666.142 pts</pdfPageSize></qualityIndicators><title>DNA Oligonucleotides and Plasmids Perform Equally as Donors for Targeted Gene Conversion</title><genre><json:string>research-article</json:string></genre><host><title>Biochemical Genetics</title><language><json:string>unknown</json:string></language><publicationDate>2010</publicationDate><copyrightDate>2010</copyrightDate><issn><json:string>0006-2928</json:string></issn><eissn><json:string>1573-4927</json:string></eissn><journalId><json:string>10528</json:string></journalId><volume>48</volume><issue>11-12</issue><pages><first>897</first><last>908</last></pages><genre><json:string>journal</json:string></genre><subject><json:item><value>Medical Microbiology</value></json:item><json:item><value>Zoology</value></json:item><json:item><value>Biochemistry, general</value></json:item><json:item><value>Human Genetics</value></json:item></subject></host><ark><json:string>ark:/67375/VQC-X6FX9VZK-Z</json:string></ark><publicationDate>2010</publicationDate><copyrightDate>2010</copyrightDate><doi><json:string>10.1007/s10528-010-9370-z</json:string></doi><id>4199191EDDA4C1C8FE9921F6EB4A0BED938224E2</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-X6FX9VZK-Z/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-X6FX9VZK-Z/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/VQC-X6FX9VZK-Z/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">DNA Oligonucleotides and Plasmids Perform Equally as Donors for Targeted Gene Conversion</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">Springer US; http://www.springer-ny.com</publisher><pubPlace>Boston</pubPlace><availability><licence><p>Springer Science+Business Media, LLC, 2010</p></licence><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</p></availability><date>2009-10-05</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">DNA Oligonucleotides and Plasmids Perform Equally as Donors for Targeted Gene Conversion</title><author xml:id="author-0000" corresp="yes"><persName><forename type="first">Stefan</forename><surname>Wagner</surname></persName><email>stefan.wagner@agresearch.co.nz</email><affiliation>Reproductive Technologies, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton, New Zealand</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Judi</forename><surname>McCracken</surname></persName><affiliation>Reproductive Technologies, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton, New Zealand</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Sally</forename><surname>Cole</surname></persName><affiliation>Reproductive Technologies, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton, New Zealand</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Götz</forename><surname>Laible</surname></persName><affiliation>Reproductive Technologies, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton, New Zealand</affiliation></author><idno type="istex">4199191EDDA4C1C8FE9921F6EB4A0BED938224E2</idno><idno type="ark">ark:/67375/VQC-X6FX9VZK-Z</idno><idno type="DOI">10.1007/s10528-010-9370-z</idno><idno type="article-id">9370</idno><idno type="article-id">s10528-010-9370-z</idno></analytic><monogr><title level="j">Biochemical Genetics</title><title level="j" type="abbrev">Biochem Genet</title><idno type="pISSN">0006-2928</idno><idno type="eISSN">1573-4927</idno><idno type="journal-ID">true</idno><idno type="issue-article-count">12</idno><idno type="volume-issue-count">12</idno><imprint><publisher>Springer US; http://www.springer-ny.com</publisher><pubPlace>Boston</pubPlace><date type="published" when="2010-12-01"></date><biblScope unit="volume">48</biblScope><biblScope unit="issue">11-12</biblScope><biblScope unit="page" from="897">897</biblScope><biblScope unit="page" to="908">908</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2009-10-05</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Site-specific gene modifications in cells are initiated by the introduction of exogenous DNA. We used a recently established cell assay to compare the ability of DNA donors to induce a single point mutation that converts a target gene encoding blue fluorescent protein (BFP) into expressing green fluorescent protein (GFP). In a chromosomal assay with cells stably expressing BFP, we showed that fluorescently labeled single-stranded oligonucleotides and a donor plasmid cotranscribing a red fluorescent protein provide similar efficiencies in triggering BFP–GFP conversions. In transient cotransfections, an isogenic donor plasmid comprising a nonfunctional GFP gene yielded a greater efficiency for the conversion of the BFP target gene than a nonisogenic donor, and all plasmid donors were superior to oligonucleotides.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Gene conversion</term></item><item><term>Green fluorescent protein</term></item><item><term>Plasmid</term></item><item><term>Oligonucleotide</term></item><item><term>Transfection</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>Biomedicine</head><item><term>Medical Microbiology</term></item><item><term>Zoology</term></item><item><term>Biochemistry, general</term></item><item><term>Human Genetics</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2009-10-05">Created</change><change when="2010-12-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-X6FX9VZK-Z/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus springer-journals not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" name="istex:docType"></istex:docType><istex:document><Publisher><PublisherInfo><PublisherName>Springer US</PublisherName><PublisherLocation>Boston</PublisherLocation><PublisherURL>http://www.springer-ny.com</PublisherURL></PublisherInfo><Journal OutputMedium="All"><JournalInfo JournalProductType="ArchiveJournal" NumberingStyle="Unnumbered"><JournalID>10528</JournalID><JournalPrintISSN>0006-2928</JournalPrintISSN><JournalElectronicISSN>1573-4927</JournalElectronicISSN><JournalTitle>Biochemical Genetics</JournalTitle><JournalAbbreviatedTitle>Biochem Genet</JournalAbbreviatedTitle><JournalSubjectGroup><JournalSubject Type="Primary">Biomedicine</JournalSubject><JournalSubject Type="Secondary">Medical Microbiology </JournalSubject><JournalSubject Type="Secondary">Zoology </JournalSubject><JournalSubject Type="Secondary">Biochemistry, general</JournalSubject><JournalSubject Type="Secondary">Human Genetics </JournalSubject></JournalSubjectGroup></JournalInfo><Volume OutputMedium="All"><VolumeInfo TocLevels="0" VolumeType="Regular"><VolumeIDStart>48</VolumeIDStart><VolumeIDEnd>48</VolumeIDEnd><VolumeIssueCount>12</VolumeIssueCount></VolumeInfo><Issue IssueType="Combined" OutputMedium="All"><IssueInfo IssueType="Combined" TocLevels="0"><IssueIDStart>11</IssueIDStart><IssueIDEnd>12</IssueIDEnd><IssueArticleCount>12</IssueArticleCount><IssueHistory><OnlineDate><Year>2010</Year><Month>11</Month><Day>17</Day></OnlineDate><PrintDate><Year>2010</Year><Month>11</Month><Day>16</Day></PrintDate><CoverDate><Year>2010</Year><Month>12</Month></CoverDate><PricelistYear>2010</PricelistYear></IssueHistory><IssueCopyright><CopyrightHolderName>Springer Science+Business Media, LLC</CopyrightHolderName><CopyrightYear>2010</CopyrightYear></IssueCopyright></IssueInfo><Article ID="s10528-010-9370-z" OutputMedium="All"><ArticleInfo ArticleType="OriginalPaper" ContainsESM="No" Language="En" NumberingStyle="Unnumbered" TocLevels="0"><ArticleID>9370</ArticleID><ArticleDOI>10.1007/s10528-010-9370-z</ArticleDOI><ArticleSequenceNumber>1</ArticleSequenceNumber><ArticleTitle Language="En" OutputMedium="All">DNA Oligonucleotides and Plasmids Perform Equally as Donors for Targeted Gene Conversion</ArticleTitle><ArticleFirstPage>897</ArticleFirstPage><ArticleLastPage>908</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2010</Year><Month>7</Month><Day>13</Day></RegistrationDate><Received><Year>2009</Year><Month>10</Month><Day>5</Day></Received><Accepted><Year>2010</Year><Month>4</Month><Day>26</Day></Accepted><OnlineDate><Year>2010</Year><Month>7</Month><Day>31</Day></OnlineDate></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer Science+Business Media, LLC</CopyrightHolderName><CopyrightYear>2010</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Stefan</GivenName><FamilyName>Wagner</FamilyName></AuthorName><Contact><Email>stefan.wagner@agresearch.co.nz</Email></Contact></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Judi</GivenName><FamilyName>McCracken</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Sally</GivenName><FamilyName>Cole</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Götz</GivenName><FamilyName>Laible</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgDivision>Reproductive Technologies</OrgDivision><OrgName>AgResearch, Ruakura Research Centre</OrgName><OrgAddress><Street>Private Bag 3123</Street><City>Hamilton</City><Country Code="NZ">New Zealand</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En" OutputMedium="All"><Heading>Abstract</Heading><Para>Site-specific gene modifications in cells are initiated by the introduction of exogenous DNA. We used a recently established cell assay to compare the ability of DNA donors to induce a single point mutation that converts a target gene encoding blue fluorescent protein (BFP) into expressing green fluorescent protein (GFP). In a chromosomal assay with cells stably expressing BFP, we showed that fluorescently labeled single-stranded oligonucleotides and a donor plasmid cotranscribing a red fluorescent protein provide similar efficiencies in triggering BFP–GFP conversions. In transient cotransfections, an isogenic donor plasmid comprising a nonfunctional GFP gene yielded a greater efficiency for the conversion of the BFP target gene than a nonisogenic donor, and all plasmid donors were superior to oligonucleotides.</Para></Abstract><KeywordGroup Language="En" OutputMedium="All"><Heading>Keywords</Heading><Keyword>Gene conversion</Keyword><Keyword>Green fluorescent protein</Keyword><Keyword>Plasmid</Keyword><Keyword>Oligonucleotide</Keyword><Keyword>Transfection</Keyword></KeywordGroup></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>DNA Oligonucleotides and Plasmids Perform Equally as Donors for Targeted Gene Conversion</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>DNA Oligonucleotides and Plasmids Perform Equally as Donors for Targeted Gene Conversion</title></titleInfo><name type="personal" displayLabel="corresp"><namePart type="given">Stefan</namePart><namePart type="family">Wagner</namePart><affiliation>Reproductive Technologies, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton, New Zealand</affiliation><affiliation>E-mail: stefan.wagner@agresearch.co.nz</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Judi</namePart><namePart type="family">McCracken</namePart><affiliation>Reproductive Technologies, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton, New Zealand</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Sally</namePart><namePart type="family">Cole</namePart><affiliation>Reproductive Technologies, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton, New Zealand</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Götz</namePart><namePart type="family">Laible</namePart><affiliation>Reproductive Technologies, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton, New Zealand</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer US; http://www.springer-ny.com</publisher><place><placeTerm type="text">Boston</placeTerm></place><dateCreated encoding="w3cdtf">2009-10-05</dateCreated><dateIssued encoding="w3cdtf">2010-12-01</dateIssued><copyrightDate encoding="w3cdtf">2010</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: Site-specific gene modifications in cells are initiated by the introduction of exogenous DNA. We used a recently established cell assay to compare the ability of DNA donors to induce a single point mutation that converts a target gene encoding blue fluorescent protein (BFP) into expressing green fluorescent protein (GFP). In a chromosomal assay with cells stably expressing BFP, we showed that fluorescently labeled single-stranded oligonucleotides and a donor plasmid cotranscribing a red fluorescent protein provide similar efficiencies in triggering BFP–GFP conversions. In transient cotransfections, an isogenic donor plasmid comprising a nonfunctional GFP gene yielded a greater efficiency for the conversion of the BFP target gene than a nonisogenic donor, and all plasmid donors were superior to oligonucleotides.</abstract><subject lang="en"><genre>Keywords</genre><topic>Gene conversion</topic><topic>Green fluorescent protein</topic><topic>Plasmid</topic><topic>Oligonucleotide</topic><topic>Transfection</topic></subject><relatedItem type="host"><titleInfo><title>Biochemical Genetics</title></titleInfo><titleInfo type="abbreviated"><title>Biochem Genet</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">2010-11-17</dateIssued><copyrightDate encoding="w3cdtf">2010</copyrightDate></originInfo><subject><genre>Biomedicine</genre><topic>Medical Microbiology</topic><topic>Zoology</topic><topic>Biochemistry, general</topic><topic>Human Genetics</topic></subject><identifier type="ISSN">0006-2928</identifier><identifier type="eISSN">1573-4927</identifier><identifier type="JournalID">10528</identifier><identifier type="IssueArticleCount">12</identifier><identifier type="VolumeIssueCount">12</identifier><part><date>2010</date><detail type="volume"><number>48</number><caption>vol.</caption></detail><detail type="issue"><number>11-12</number><caption>no.</caption></detail><extent unit="pages"><start>897</start><end>908</end></extent></part><recordInfo><recordOrigin>Springer Science+Business Media, LLC, 2010</recordOrigin></recordInfo></relatedItem><identifier type="istex">4199191EDDA4C1C8FE9921F6EB4A0BED938224E2</identifier><identifier type="ark">ark:/67375/VQC-X6FX9VZK-Z</identifier><identifier type="DOI">10.1007/s10528-010-9370-z</identifier><identifier type="ArticleID">9370</identifier><identifier type="ArticleID">s10528-010-9370-z</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer Science+Business Media, LLC, 2010</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Springer Science+Business Media, LLC, 2010</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-X6FX9VZK-Z/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000C11 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000C11 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:4199191EDDA4C1C8FE9921F6EB4A0BED938224E2
|texte= DNA Oligonucleotides and Plasmids Perform Equally as Donors for Targeted Gene Conversion
}}
| This area was generated with Dilib version V0.6.33. |  |