Nucleotide sequence of a 1446 base pair SalI fragment and structure of a novel early gene of Leucania seperata nuclear polyhedrosis virus
Identifieur interne : 000C10 ( Istex/Corpus ); précédent : 000C09; suivant : 000C11Nucleotide sequence of a 1446 base pair SalI fragment and structure of a novel early gene of Leucania seperata nuclear polyhedrosis virus
Auteurs : J. W. Wang ; Y. P. Qi ; Y. X. Huang ; S. D. LiSource :
- Archives of Virology [ 0304-8608 ] ; 1995-12-01.
English descriptors
- Teeft :
- Acetic acid, Amino, Amino acid, Amino acid residue, Amino acid residues, Amino acids, Autographa, Autographa californica, Baculovirus, Baculovirus gene, Baculovirus vector, Bombyx mori, Cagt motif, Californica, Cgtcg motif, Complete sequence, Core recognition sequence, Early gene, Early genes, Early promoter, Functional mapping, Gata, Gata motif, Gata motifs, Gene, Gene expression, Genome, Insect cells, Late phases, Late promoter sequence ttaag, Late promoters, Leucania seperata, Leucine, Leucine structure, Leucine zipper, Lsnpv, Lsnpv genome, Mini, Mini cistron, Molecular biology, Nucleotide, Nucleotide sequence, Other elements, Polyhedrosis, Polyhedrosis virus, Promoter, Prosis software, Putative protein, Random sequence, Recombinant plasmids, Regular leucine structures, Regulatory elements, Restriction sites, Sali, Sali fragment, Sali sites, Secondary structure, Sequencing, Small peptide, Tata, Tata boxes, Termination codon, Transcription, Tryptophane operon, Virol, Virology, Wuhan university.
Abstract
Summary: A 1446 bp SalI fragment of LsNPV was sequenced by the silver staining method, and two large open reading frames (ORFs, ORF1 and ORF2) were found, both contain typical characteristics of the 5′ regulatory elements of baculovirus early genes. ORF1 is 345 bp long with the capacity to encode a putative protein of 114 amino acid residues with MW about 13 kDa and was designated p13 gene, ORF2 comprises 248 bp from the 3′ end of the fragment. In the untranslated region (UTR) of ORF1, a 33 bp mini cistron (ORF3), a core recognition sequence (CGTCG) for many bHLHzip transcription factors and a late promoter sequence TTAAG are present. In the UTR of ORF2, two host transcription factor binding elements (CACGTG and GATA motif) and two CGT motifs were found. Some regular leucine zipper-like structures, designated leucine trans-conformation structure and LVT repeat, were found near the N-terminus and the middle of p13 protein. The leucine trans-conformation structure that is near the N-terminus consists of 4 leucines and 7 other amino acids between every two leucines, and every leucine is located at a conformation shift point of the predicted secondary structure of the p13 protein. In LVT repeat, L-6aa-V-6aa-T-6aa is repeated once. The functions of those structures remain unclear, and the two ORFs, not found in the genome ofAutographa californica nuclear polyhedrosis virus, are possibly two new genes.
Url:
DOI: 10.1007/BF01323248
Links to Exploration step
ISTEX:4F3D6657EE30E88125C2F1783FDFCF1344A45559Le document en format XML
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Wang</name><affiliation><mods:affiliation>Institute of Virology, Wuhan University, Wuhan, China</mods:affiliation></affiliation></author><author><name sortKey="Qi, Y P" sort="Qi, Y P" uniqKey="Qi Y" first="Y. P." last="Qi">Y. P. Qi</name><affiliation><mods:affiliation>Institute of Virology, Wuhan University, Wuhan, China</mods:affiliation></affiliation></author><author><name sortKey="Huang, Y X" sort="Huang, Y X" uniqKey="Huang Y" first="Y. X." last="Huang">Y. X. Huang</name><affiliation><mods:affiliation>Institute of Virology, Wuhan University, Wuhan, China</mods:affiliation></affiliation></author><author><name sortKey="Li, S D" sort="Li, S D" uniqKey="Li S" first="S. D." last="Li">S. D. Li</name><affiliation><mods:affiliation>Institute of Virology, Wuhan University, Wuhan, China</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Archives of Virology</title><title level="j" type="abbrev">Archives of Virology</title><idno type="ISSN">0304-8608</idno><idno type="eISSN">1432-8798</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Vienna</pubPlace><date type="published" when="1995-12-01">1995-12-01</date><biblScope unit="volume">140</biblScope><biblScope unit="issue">12</biblScope><biblScope unit="page" from="2283">2283</biblScope><biblScope unit="page" to="2291">2291</biblScope></imprint><idno type="ISSN">0304-8608</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0304-8608</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acetic acid</term><term>Amino</term><term>Amino acid</term><term>Amino acid residue</term><term>Amino acid residues</term><term>Amino acids</term><term>Autographa</term><term>Autographa californica</term><term>Baculovirus</term><term>Baculovirus gene</term><term>Baculovirus vector</term><term>Bombyx mori</term><term>Cagt motif</term><term>Californica</term><term>Cgtcg motif</term><term>Complete sequence</term><term>Core recognition sequence</term><term>Early gene</term><term>Early genes</term><term>Early promoter</term><term>Functional mapping</term><term>Gata</term><term>Gata motif</term><term>Gata motifs</term><term>Gene</term><term>Gene expression</term><term>Genome</term><term>Insect cells</term><term>Late phases</term><term>Late promoter sequence ttaag</term><term>Late promoters</term><term>Leucania seperata</term><term>Leucine</term><term>Leucine structure</term><term>Leucine zipper</term><term>Lsnpv</term><term>Lsnpv genome</term><term>Mini</term><term>Mini cistron</term><term>Molecular biology</term><term>Nucleotide</term><term>Nucleotide sequence</term><term>Other elements</term><term>Polyhedrosis</term><term>Polyhedrosis virus</term><term>Promoter</term><term>Prosis software</term><term>Putative protein</term><term>Random sequence</term><term>Recombinant plasmids</term><term>Regular leucine structures</term><term>Regulatory elements</term><term>Restriction sites</term><term>Sali</term><term>Sali fragment</term><term>Sali sites</term><term>Secondary structure</term><term>Sequencing</term><term>Small peptide</term><term>Tata</term><term>Tata boxes</term><term>Termination codon</term><term>Transcription</term><term>Tryptophane operon</term><term>Virol</term><term>Virology</term><term>Wuhan university</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: A 1446 bp SalI fragment of LsNPV was sequenced by the silver staining method, and two large open reading frames (ORFs, ORF1 and ORF2) were found, both contain typical characteristics of the 5′ regulatory elements of baculovirus early genes. ORF1 is 345 bp long with the capacity to encode a putative protein of 114 amino acid residues with MW about 13 kDa and was designated p13 gene, ORF2 comprises 248 bp from the 3′ end of the fragment. In the untranslated region (UTR) of ORF1, a 33 bp mini cistron (ORF3), a core recognition sequence (CGTCG) for many bHLHzip transcription factors and a late promoter sequence TTAAG are present. In the UTR of ORF2, two host transcription factor binding elements (CACGTG and GATA motif) and two CGT motifs were found. Some regular leucine zipper-like structures, designated leucine trans-conformation structure and LVT repeat, were found near the N-terminus and the middle of p13 protein. The leucine trans-conformation structure that is near the N-terminus consists of 4 leucines and 7 other amino acids between every two leucines, and every leucine is located at a conformation shift point of the predicted secondary structure of the p13 protein. In LVT repeat, L-6aa-V-6aa-T-6aa is repeated once. The functions of those structures remain unclear, and the two ORFs, not found in the genome ofAutographa californica nuclear polyhedrosis virus, are possibly two new genes.</div></front></TEI><istex><corpusName>springer-journals</corpusName><keywords><teeft><json:string>leucine</json:string><json:string>baculovirus</json:string><json:string>polyhedrosis</json:string><json:string>lsnpv</json:string><json:string>polyhedrosis virus</json:string><json:string>gata</json:string><json:string>virol</json:string><json:string>genome</json:string><json:string>sali</json:string><json:string>autographa</json:string><json:string>virology</json:string><json:string>californica</json:string><json:string>tata</json:string><json:string>amino</json:string><json:string>promoter</json:string><json:string>sequencing</json:string><json:string>autographa californica</json:string><json:string>mini</json:string><json:string>amino acid residues</json:string><json:string>leucine structure</json:string><json:string>nucleotide sequence</json:string><json:string>gata motif</json:string><json:string>amino acids</json:string><json:string>transcription</json:string><json:string>gene</json:string><json:string>regulatory elements</json:string><json:string>molecular biology</json:string><json:string>complete sequence</json:string><json:string>mini cistron</json:string><json:string>bombyx mori</json:string><json:string>secondary structure</json:string><json:string>prosis software</json:string><json:string>early gene</json:string><json:string>termination codon</json:string><json:string>leucine zipper</json:string><json:string>nucleotide</json:string><json:string>gene expression</json:string><json:string>sali fragment</json:string><json:string>other elements</json:string><json:string>early genes</json:string><json:string>late phases</json:string><json:string>late promoter sequence ttaag</json:string><json:string>lsnpv genome</json:string><json:string>recombinant plasmids</json:string><json:string>leucania seperata</json:string><json:string>acetic acid</json:string><json:string>baculovirus vector</json:string><json:string>restriction sites</json:string><json:string>sali sites</json:string><json:string>cgtcg motif</json:string><json:string>late promoters</json:string><json:string>wuhan university</json:string><json:string>regular leucine structures</json:string><json:string>small peptide</json:string><json:string>tata boxes</json:string><json:string>gata motifs</json:string><json:string>core recognition sequence</json:string><json:string>putative protein</json:string><json:string>amino acid</json:string><json:string>amino acid residue</json:string><json:string>cagt motif</json:string><json:string>random sequence</json:string><json:string>early promoter</json:string><json:string>tryptophane operon</json:string><json:string>functional mapping</json:string><json:string>baculovirus gene</json:string><json:string>insect cells</json:string></teeft></keywords><author><json:item><name>J. W. Wang</name><affiliations><json:string>Institute of Virology, Wuhan University, Wuhan, China</json:string></affiliations></json:item><json:item><name>Y. P. Qi</name><affiliations><json:string>Institute of Virology, Wuhan University, Wuhan, China</json:string></affiliations></json:item><json:item><name>Y. X. Huang</name><affiliations><json:string>Institute of Virology, Wuhan University, Wuhan, China</json:string></affiliations></json:item><json:item><name>S. D. Li</name><affiliations><json:string>Institute of Virology, Wuhan University, Wuhan, China</json:string></affiliations></json:item></author><articleId><json:string>BF01323248</json:string><json:string>Art15</json:string></articleId><arkIstex>ark:/67375/1BB-KZ85176D-Q</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>BriefCommunication</json:string></originalGenre><abstract>Summary: A 1446 bp SalI fragment of LsNPV was sequenced by the silver staining method, and two large open reading frames (ORFs, ORF1 and ORF2) were found, both contain typical characteristics of the 5′ regulatory elements of baculovirus early genes. ORF1 is 345 bp long with the capacity to encode a putative protein of 114 amino acid residues with MW about 13 kDa and was designated p13 gene, ORF2 comprises 248 bp from the 3′ end of the fragment. In the untranslated region (UTR) of ORF1, a 33 bp mini cistron (ORF3), a core recognition sequence (CGTCG) for many bHLHzip transcription factors and a late promoter sequence TTAAG are present. In the UTR of ORF2, two host transcription factor binding elements (CACGTG and GATA motif) and two CGT motifs were found. Some regular leucine zipper-like structures, designated leucine trans-conformation structure and LVT repeat, were found near the N-terminus and the middle of p13 protein. The leucine trans-conformation structure that is near the N-terminus consists of 4 leucines and 7 other amino acids between every two leucines, and every leucine is located at a conformation shift point of the predicted secondary structure of the p13 protein. In LVT repeat, L-6aa-V-6aa-T-6aa is repeated once. 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ORF1 is 345 bp long with the capacity to encode a putative protein of 114 amino acid residues with MW about 13 kDa and was designated p13 gene, ORF2 comprises 248 bp from the 3′ end of the fragment. In the untranslated region (UTR) of ORF1, a 33 bp mini cistron (ORF3), a core recognition sequence (CGTCG) for many bHLHzip transcription factors and a late promoter sequence TTAAG are present. In the UTR of ORF2, two host transcription factor binding elements (CACGTG and GATA motif) and two CGT motifs were found. Some regular leucine zipper-like structures, designated leucine trans-conformation structure and LVT repeat, were found near the N-terminus and the middle of p13 protein. The leucine trans-conformation structure that is near the N-terminus consists of 4 leucines and 7 other amino acids between every two leucines, and every leucine is located at a conformation shift point of the predicted secondary structure of the p13 protein. In LVT repeat, L-6aa-V-6aa-T-6aa is repeated once. The functions of those structures remain unclear, and the two ORFs, not found in the genome ofAutographa californica nuclear polyhedrosis virus, are possibly two new genes.</p></abstract><textClass><keywords scheme="Journal Subject"><list><head>Biomedicine</head><item><term>Medical Microbiology</term></item><item><term>Virology</term></item><item><term>Infectious Diseases</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1995-03-23">Created</change><change when="1995-12-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/1BB-KZ85176D-Q/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus springer-journals not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ 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virus</ArticleTitle><ArticleCategory>Brief Report</ArticleCategory><ArticleFirstPage>2283</ArticleFirstPage><ArticleLastPage>2291</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2005</Year><Month>3</Month><Day>4</Day></RegistrationDate><Received><Year>1995</Year><Month>3</Month><Day>23</Day></Received><Accepted><Year>1995</Year><Month>7</Month><Day>6</Day></Accepted></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>1995</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants><ArticleContext><JournalID>705</JournalID><VolumeIDStart>140</VolumeIDStart><VolumeIDEnd>140</VolumeIDEnd><IssueIDStart>12</IssueIDStart><IssueIDEnd>12</IssueIDEnd></ArticleContext></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1" PresentAffiliationID="Aff2"><AuthorName DisplayOrder="Western"><GivenName>J.</GivenName><GivenName>W.</GivenName><FamilyName>Wang</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Y.</GivenName><GivenName>P.</GivenName><FamilyName>Qi</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Y.</GivenName><GivenName>X.</GivenName><FamilyName>Huang</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>S.</GivenName><GivenName>D.</GivenName><FamilyName>Li</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgDivision>Institute of Virology</OrgDivision><OrgName>Wuhan University</OrgName><OrgAddress><City>Wuhan</City><Country>China</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>National Laboratory of Molecular Oncology, Cancer Institute of Peking Union Medical College</OrgDivision><OrgName>Chinese Academy of Medical Sciences</OrgName><OrgAddress><City>Beijing</City><Country>China</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Summary</Heading><Para>A 1446 bp SalI fragment of LsNPV was sequenced by the silver staining method, and two large open reading frames (ORFs, ORF1 and ORF2) were found, both contain typical characteristics of the 5′ regulatory elements of baculovirus early genes. ORF1 is 345 bp long with the capacity to encode a putative protein of 114 amino acid residues with MW about 13 kDa and was designated p13 gene, ORF2 comprises 248 bp from the 3′ end of the fragment. In the untranslated region (UTR) of ORF1, a 33 bp mini cistron (ORF3), a core recognition sequence (CGTCG) for many bHLHzip transcription factors and a late promoter sequence TTAAG are present. In the UTR of ORF2, two host transcription factor binding elements (CACGTG and GATA motif) and two CGT motifs were found. Some regular leucine zipper-like structures, designated leucine trans-conformation structure and LVT repeat, were found near the N-terminus and the middle of p13 protein. The leucine trans-conformation structure that is near the N-terminus consists of 4 leucines and 7 other amino acids between every two leucines, and every leucine is located at a conformation shift point of the predicted secondary structure of the p13 protein. In LVT repeat, L-6aa-V-6aa-T-6aa is repeated once. The functions of those structures remain unclear, and the two ORFs, not found in the genome of<Emphasis Type="Italic">Autographa californica</Emphasis> nuclear polyhedrosis virus, are possibly two new genes.</Para></Abstract><ArticleNote Type="Misc"><SimplePara>This work was supported by Chinese National Foundation of Natural Science. The GenBank accession number of this nucleotide sequence is U30303.</SimplePara></ArticleNote></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Nucleotide sequence of a 1446 base pair SalI fragment and structure of a novel early gene of Leucania seperata nuclear polyhedrosis virus</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Nucleotide sequence of a 1446 base pair SalI fragment and structure of a novel early gene of Leucania seperata nuclear polyhedrosis virus</title></titleInfo><name type="personal"><namePart type="given">J.</namePart><namePart type="given">W.</namePart><namePart type="family">Wang</namePart><affiliation>Institute of Virology, Wuhan University, Wuhan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Y.</namePart><namePart type="given">P.</namePart><namePart type="family">Qi</namePart><affiliation>Institute of Virology, Wuhan University, Wuhan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Y.</namePart><namePart type="given">X.</namePart><namePart type="family">Huang</namePart><affiliation>Institute of Virology, Wuhan University, Wuhan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="given">D.</namePart><namePart type="family">Li</namePart><affiliation>Institute of Virology, Wuhan University, Wuhan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="brief-communication" displayLabel="BriefCommunication" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-S9SX2MFS-0">brief-communication</genre><originInfo><publisher>Springer-Verlag</publisher><place><placeTerm type="text">Vienna</placeTerm></place><dateCreated encoding="w3cdtf">1995-03-23</dateCreated><dateIssued encoding="w3cdtf">1995-12-01</dateIssued><copyrightDate encoding="w3cdtf">1995</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Summary: A 1446 bp SalI fragment of LsNPV was sequenced by the silver staining method, and two large open reading frames (ORFs, ORF1 and ORF2) were found, both contain typical characteristics of the 5′ regulatory elements of baculovirus early genes. ORF1 is 345 bp long with the capacity to encode a putative protein of 114 amino acid residues with MW about 13 kDa and was designated p13 gene, ORF2 comprises 248 bp from the 3′ end of the fragment. In the untranslated region (UTR) of ORF1, a 33 bp mini cistron (ORF3), a core recognition sequence (CGTCG) for many bHLHzip transcription factors and a late promoter sequence TTAAG are present. In the UTR of ORF2, two host transcription factor binding elements (CACGTG and GATA motif) and two CGT motifs were found. Some regular leucine zipper-like structures, designated leucine trans-conformation structure and LVT repeat, were found near the N-terminus and the middle of p13 protein. The leucine trans-conformation structure that is near the N-terminus consists of 4 leucines and 7 other amino acids between every two leucines, and every leucine is located at a conformation shift point of the predicted secondary structure of the p13 protein. In LVT repeat, L-6aa-V-6aa-T-6aa is repeated once. The functions of those structures remain unclear, and the two ORFs, not found in the genome ofAutographa californica nuclear polyhedrosis virus, are possibly two new genes.</abstract><note>Brief Report</note><relatedItem type="host"><titleInfo><title>Archives of Virology</title></titleInfo><titleInfo type="abbreviated"><title>Archives of Virology</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">1995-12-01</dateIssued><copyrightDate encoding="w3cdtf">1995</copyrightDate></originInfo><subject><genre>Biomedicine</genre><topic>Medical Microbiology</topic><topic>Virology</topic><topic>Infectious Diseases</topic></subject><identifier type="ISSN">0304-8608</identifier><identifier type="eISSN">1432-8798</identifier><identifier type="JournalID">705</identifier><identifier type="IssueArticleCount">16</identifier><identifier type="VolumeIssueCount">12</identifier><part><date>1995</date><detail type="volume"><number>140</number><caption>vol.</caption></detail><detail type="issue"><number>12</number><caption>no.</caption></detail><extent unit="pages"><start>2283</start><end>2291</end></extent></part><recordInfo><recordOrigin>Springer-Verlag, 1995</recordOrigin></recordInfo></relatedItem><identifier type="istex">4F3D6657EE30E88125C2F1783FDFCF1344A45559</identifier><identifier type="ark">ark:/67375/1BB-KZ85176D-Q</identifier><identifier type="DOI">10.1007/BF01323248</identifier><identifier type="ArticleID">BF01323248</identifier><identifier type="ArticleID">Art15</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer-Verlag, 1995</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Springer-Verlag, 1995</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/1BB-KZ85176D-Q/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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