MersV1, Istex, Corpus, bibRecord, 000C06

Messenger RNA expression for a TSH receptor variant in the thymus of a two-year-old child

Identifieur interne : 000C06 ( Istex/Corpus ); précédent : 000C05; suivant : 000C07

Messenger RNA expression for a TSH receptor variant in the thymus of a two-year-old child

Auteurs : R. Paschke ; V. Geenen

Source :

Journal of Molecular Medicine [ 0946-2716 ] ; 1995-11-01.

RBID : ISTEX:E1CF6EE036DBB02E50E9161E94D7737C8E519C82

English descriptors

KwdEn :
- Thymus, Thyroid-associated ophthalmopathy, Thyroid-stimulating hormone receptor, Thyroid-stimulating hormone receptor variant, mRNA expression.
Teeft :
- Amplification, Amplification product, Amplification products, Anal biochem, Biochem biophys, Cdna, Cdna template, Clin endocrinol metab, Endocrinol, Epithelial, Epithelial thymic cdna library, Epithelial thymus library, Exon, Extracellular domain, Hormone receptor variant, Human thymus, Intracellular part, Ludgate, Luteinizing hormone, Magnetic beads, Mrna, Negative control, Normal thyroid, Normal thyroid cdna library, Ophthalmopathy, Previous studies, Previous study, Primer, Receptor, Receptor variant, Size marker, Tail sequence, Thymic, Thymus, Thymus cdna, Thymus epithelium, Thymus tissue, Thyrotropin receptor, Tolerance induction, Transcript, Variant, Variant transcript, Variant transcripts, Vassart, Vector pbsk.

Abstract

Abstract: We recently described the presence of a thyroid-stimulating hormone receptor (TSH-R) variant in orbital tissues. Although the presence of this TSH-R variant could provide the antigenic link between the thyroid and the orbit in Graves' disease and thyroid-associated ophthalmopathy (TAO), the etiopathophysiological significance of this finding remains to be elucidated. Graves' disease and TAO are autoimmune diseases which are likely to be caused by a breakdown of tolerance. We therefore investigated the presence of this variant TSH-R transcript in human thymus. Using primers specific for this variant in reverse polymerase chain reaction (PCR) experiments, Southern blotting, and sequencing of the PCR products we demonstrate the presence of this transcript in RNA extracted from normal human thymus of a 2-year-old child. We were also able to amplify this variant TSH-R transcript from a normal human thyroid cDNA library, but not from an epithelial thymus library. The presence of the variant TSH-R transcript in RNA prepared from normal human thymus suggests that induction of immunological tolerance against this variant TSH-R transcript in the thymus is possible. A lack of tolerance induction for this variant TSH-R transcript could provide an explanation for a possible antigenic link between Graves' disease and TAO.

Url:

https://api.istex.fr/ark:/67375/1BB-6WCC2KSM-3/fulltext.pdf

DOI: 10.1007/BF00195143

Links to Exploration step

ISTEX:E1CF6EE036DBB02E50E9161E94D7737C8E519C82

Le document en format XML

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<term>Thyroid-associated ophthalmopathy</term>
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<ArticleTitle Language="En">Messenger RNA expression for a TSH receptor variant in the thymus of a two-year-old child</ArticleTitle>
<ArticleCategory>Hypothesis</ArticleCategory>
<ArticleFirstPage>577</ArticleFirstPage>
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<Month>7</Month>
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<VolumeIDStart>73</VolumeIDStart>
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<IssueIDStart>11</IssueIDStart>
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<ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>R.</GivenName>
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<Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>V.</GivenName>
<FamilyName>Geenen</FamilyName>
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<Affiliation ID="Aff1"><OrgDivision>Medizinische Klinik und Poliklinik III</OrgDivision>
<OrgName>Universität Leipzig</OrgName>
<OrgAddress><Street>Ph. Rosenthal Strasse 27</Street>
<Postcode>D-04103</Postcode>
<City>Leipzig</City>
<Country>Germany</Country>
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<Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading>
<Para>We recently described the presence of a thyroid-stimulating hormone receptor (TSH-R) variant in orbital tissues. Although the presence of this TSH-R variant could provide the antigenic link between the thyroid and the orbit in Graves' disease and thyroid-associated ophthalmopathy (TAO), the etiopathophysiological significance of this finding remains to be elucidated. Graves' disease and TAO are autoimmune diseases which are likely to be caused by a breakdown of tolerance. We therefore investigated the presence of this variant TSH-R transcript in human thymus. Using primers specific for this variant in reverse polymerase chain reaction (PCR) experiments, Southern blotting, and sequencing of the PCR products we demonstrate the presence of this transcript in RNA extracted from normal human thymus of a 2-year-old child. We were also able to amplify this variant TSH-R transcript from a normal human thyroid cDNA library, but not from an epithelial thymus library. The presence of the variant TSH-R transcript in RNA prepared from normal human thymus suggests that induction of immunological tolerance against this variant TSH-R transcript in the thymus is possible. A lack of tolerance induction for this variant TSH-R transcript could provide an explanation for a possible antigenic link between Graves' disease and TAO.</Para>
</Abstract>
<KeywordGroup Language="En"><Heading>Key words</Heading>
<Keyword>Thyroid-associated ophthalmopathy</Keyword>
<Keyword>Thyroid-stimulating hormone receptor</Keyword>
<Keyword>Thyroid-stimulating hormone receptor variant</Keyword>
<Keyword>mRNA expression</Keyword>
<Keyword>Thymus</Keyword>
</KeywordGroup>
<AbbreviationGroup><Heading>Abbreviations</Heading>
<DefinitionList><DefinitionListEntry><Term><Emphasis Type="Italic">ECD</Emphasis>
</Term>
<Description><Para>Extracellular domain</Para>
</Description>
</DefinitionListEntry>
<DefinitionListEntry><Term><Emphasis Type="Italic">FSH</Emphasis>
</Term>
<Description><Para>Follicle-stimulating hormone</Para>
</Description>
</DefinitionListEntry>
<DefinitionListEntry><Term><Emphasis Type="Italic">ICD</Emphasis>
</Term>
<Description><Para>Intracellular domain</Para>
</Description>
</DefinitionListEntry>
<DefinitionListEntry><Term><Emphasis Type="Italic">LH</Emphasis>
</Term>
<Description><Para>Luteinizing hormone</Para>
</Description>
</DefinitionListEntry>
<DefinitionListEntry><Term><Emphasis Type="Italic">MMLV</Emphasis>
</Term>
<Description><Para>Murine myelomonocytic lymphoma virus</Para>
</Description>
</DefinitionListEntry>
<DefinitionListEntry><Term><Emphasis Type="Italic">PCR</Emphasis>
</Term>
<Description><Para>Polymerase chain reaction</Para>
</Description>
</DefinitionListEntry>
<DefinitionListEntry><Term><Emphasis Type="Italic">TAO</Emphasis>
</Term>
<Description><Para>Thyroid-associated ophthalmopathy</Para>
</Description>
</DefinitionListEntry>
<DefinitionListEntry><Term><Emphasis Type="Italic">TSH-R</Emphasis>
</Term>
<Description><Para>Thyroid-stimulating hormone receptor</Para>
</Description>
</DefinitionListEntry>
</DefinitionList>
</AbbreviationGroup>
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</Journal>
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<abstract lang="en">Abstract: We recently described the presence of a thyroid-stimulating hormone receptor (TSH-R) variant in orbital tissues. Although the presence of this TSH-R variant could provide the antigenic link between the thyroid and the orbit in Graves' disease and thyroid-associated ophthalmopathy (TAO), the etiopathophysiological significance of this finding remains to be elucidated. Graves' disease and TAO are autoimmune diseases which are likely to be caused by a breakdown of tolerance. We therefore investigated the presence of this variant TSH-R transcript in human thymus. Using primers specific for this variant in reverse polymerase chain reaction (PCR) experiments, Southern blotting, and sequencing of the PCR products we demonstrate the presence of this transcript in RNA extracted from normal human thymus of a 2-year-old child. We were also able to amplify this variant TSH-R transcript from a normal human thyroid cDNA library, but not from an epithelial thymus library. The presence of the variant TSH-R transcript in RNA prepared from normal human thymus suggests that induction of immunological tolerance against this variant TSH-R transcript in the thymus is possible. A lack of tolerance induction for this variant TSH-R transcript could provide an explanation for a possible antigenic link between Graves' disease and TAO.</abstract>
<note>Hypothesis</note>
<subject lang="en"><genre>Key words</genre>
<topic>Thyroid-associated ophthalmopathy</topic>
<topic>Thyroid-stimulating hormone receptor</topic>
<topic>Thyroid-stimulating hormone receptor variant</topic>
<topic>mRNA expression</topic>
<topic>Thymus</topic>
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<subject><genre>Abbreviations</genre>
<topic>ECD : Extracellular domain</topic>
<topic>FSH : Follicle-stimulating hormone</topic>
<topic>ICD : Intracellular domain</topic>
<topic>LH : Luteinizing hormone</topic>
<topic>MMLV : Murine myelomonocytic lymphoma virus</topic>
<topic>PCR : Polymerase chain reaction</topic>
<topic>TAO : Thyroid-associated ophthalmopathy</topic>
<topic>TSH-R : Thyroid-stimulating hormone receptor</topic>
</subject>
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Messenger RNA expression for a TSH receptor variant in the thymus of a two-year-old child

Messenger RNA expression for a TSH receptor variant in the thymus of a two-year-old child

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