Cellular oncogenes in human teratocarcinoma cell lines
Identifieur interne : 001F99 ( Istex/Checkpoint ); précédent : 001F98; suivant : 002000Cellular oncogenes in human teratocarcinoma cell lines
Auteurs : H. Tesch [Allemagne] ; R. Fürba [Allemagne] ; J. Casper [Allemagne] ; J. Lyons [Allemagne] ; C. R. Bartram [Allemagne] ; H. J. Schmoll [Allemagne] ; D. L. Bronson [États-Unis]Source :
- International Journal of Andrology [ 0105-6263 ] ; 1990-10.
Abstract
We have analysed, by Northern blots, the expression of 14 cellular oncogenes in nine cell lines established from human teratocarcinomas. All lines expressed considerable amounts of p53, c‐Ki‐ras2, c‐Ha‐ras1, craf1, N‐myc, and c‐fos. Low level expression of c‐myc was detected in some lines. Southern blot experiments revealed no amplification or rearrangement of the c‐Ki‐ras2, N‐myc or c‐fos genes. Using a rapid dot‐blot screening procedure, based on a combination of in‐vitro amplification of ras‐specific sequences and oligonucleotide hybridization, we could detect no activation of Ha‐ras or Ki‐ras or any unexpressed N‐ras sequences secondary to a point mutation at codons 12, 13, or 61.
Url:
DOI: 10.1111/j.1365-2605.1990.tb01046.x
Affiliations:
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Fürba</name><affiliation wicri:level="4"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Medizinische Klinik, Universität Köln, Köln, University of Ulm</wicri:regionArea><placeName><settlement type="city">Ulm</settlement><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Tübingen</region></placeName><orgName type="university">Université d'Ulm</orgName></affiliation></author><author><name sortKey="Casper, J" sort="Casper, J" uniqKey="Casper J" first="J." last="Casper">J. Casper</name><affiliation wicri:level="4"><country xml:lang="fr">Allemagne</country><wicri:regionArea>*Hämatologische Klinik, Medizinische Hochschule Hannover, University of Ulm</wicri:regionArea><placeName><settlement type="city">Ulm</settlement><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Tübingen</region></placeName><orgName type="university">Université d'Ulm</orgName></affiliation></author><author><name sortKey="Lyons, J" sort="Lyons, J" uniqKey="Lyons J" first="J." last="Lyons">J. Lyons</name><affiliation wicri:level="4"><country xml:lang="fr">Allemagne</country><wicri:regionArea>†Section of Molecular Biology Department of Pediatrics I, University of Ulm</wicri:regionArea><placeName><settlement type="city">Ulm</settlement><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Tübingen</region></placeName><orgName type="university">Université d'Ulm</orgName></affiliation></author><author><name sortKey="Bartram, C R" sort="Bartram, C R" uniqKey="Bartram C" first="C. R." last="Bartram">C. R. 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Schmoll</name><affiliation wicri:level="4"><country xml:lang="fr">Allemagne</country><wicri:regionArea>*Hämatologische Klinik, Medizinische Hochschule Hannover, University of Ulm</wicri:regionArea><placeName><settlement type="city">Ulm</settlement><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Tübingen</region></placeName><orgName type="university">Université d'Ulm</orgName></affiliation></author><author><name sortKey="Bronson, D L" sort="Bronson, D L" uniqKey="Bronson D" first="D. L." last="Bronson">D. L. Bronson</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>‡Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">International Journal of Andrology</title><title level="j" type="alt">INTERNATIONAL JOURNAL OF ANDROLOGY</title><idno type="ISSN">0105-6263</idno><idno type="eISSN">1365-2605</idno><imprint><biblScope unit="vol">13</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="377">377</biblScope><biblScope unit="page" to="388">388</biblScope><biblScope unit="page-count">12</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1990-10">1990-10</date></imprint><idno type="ISSN">0105-6263</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0105-6263</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have analysed, by Northern blots, the expression of 14 cellular oncogenes in nine cell lines established from human teratocarcinomas. All lines expressed considerable amounts of p53, c‐Ki‐ras2, c‐Ha‐ras1, craf1, N‐myc, and c‐fos. Low level expression of c‐myc was detected in some lines. Southern blot experiments revealed no amplification or rearrangement of the c‐Ki‐ras2, N‐myc or c‐fos genes. Using a rapid dot‐blot screening procedure, based on a combination of in‐vitro amplification of ras‐specific sequences and oligonucleotide hybridization, we could detect no activation of Ha‐ras or Ki‐ras or any unexpressed N‐ras sequences secondary to a point mutation at codons 12, 13, or 61.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>États-Unis</li></country><region><li>Bade-Wurtemberg</li><li>District de Tübingen</li><li>Texas</li></region><settlement><li>Ulm</li></settlement><orgName><li>Université d'Ulm</li></orgName></list><tree><country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Tesch, H" sort="Tesch, H" uniqKey="Tesch H" first="H." last="Tesch">H. 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