Cellular oncogenes in human teratocarcinoma cell lines
Identifieur interne : 001448 ( Istex/Curation ); précédent : 001447; suivant : 001449Cellular oncogenes in human teratocarcinoma cell lines
Auteurs : H. Tesch [Allemagne] ; R. Fürba [Allemagne] ; J. Casper [Allemagne] ; J. Lyons [Allemagne] ; C. R. Bartram [Allemagne] ; H. J. Schmoll [Allemagne] ; D. L. Bronson [États-Unis]Source :
- International Journal of Andrology [ 0105-6263 ] ; 1990-10.
Abstract
We have analysed, by Northern blots, the expression of 14 cellular oncogenes in nine cell lines established from human teratocarcinomas. All lines expressed considerable amounts of p53, c‐Ki‐ras2, c‐Ha‐ras1, craf1, N‐myc, and c‐fos. Low level expression of c‐myc was detected in some lines. Southern blot experiments revealed no amplification or rearrangement of the c‐Ki‐ras2, N‐myc or c‐fos genes. Using a rapid dot‐blot screening procedure, based on a combination of in‐vitro amplification of ras‐specific sequences and oligonucleotide hybridization, we could detect no activation of Ha‐ras or Ki‐ras or any unexpressed N‐ras sequences secondary to a point mutation at codons 12, 13, or 61.
Url:
DOI: 10.1111/j.1365-2605.1990.tb01046.x
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001448
Links to Exploration step
ISTEX:A6EB08728AA9B09FAB01BC30B98FB7BA26CEE854Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cellular oncogenes in human teratocarcinoma cell lines</title>
<author><name sortKey="Tesch, H" sort="Tesch, H" uniqKey="Tesch H" first="H." last="Tesch">H. Tesch</name>
<affiliation wicri:level="1"><mods:affiliation>Medizinische Klinik, Universität Köln, Köln, University of Ulm, FRG</mods:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Medizinische Klinik, Universität Köln, Köln, University of Ulm</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Correspondence address: Medizinische Klinik, Universität Köln, 5000 Köln 41, FRG</mods:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Correspondence address: Medizinische Klinik, Universität Köln, 5000 Köln 41</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Furba, R" sort="Furba, R" uniqKey="Furba R" first="R." last="Fürba">R. Fürba</name>
<affiliation wicri:level="1"><mods:affiliation>Medizinische Klinik, Universität Köln, Köln, University of Ulm, FRG</mods:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Medizinische Klinik, Universität Köln, Köln, University of Ulm</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Casper, J" sort="Casper, J" uniqKey="Casper J" first="J." last="Casper">J. Casper</name>
<affiliation wicri:level="1"><mods:affiliation>*Hämatologische Klinik, Medizinische Hochschule Hannover, University of Ulm, FRG</mods:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>*Hämatologische Klinik, Medizinische Hochschule Hannover, University of Ulm</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Lyons, J" sort="Lyons, J" uniqKey="Lyons J" first="J." last="Lyons">J. Lyons</name>
<affiliation wicri:level="1"><mods:affiliation>†Section of Molecular Biology Department of Pediatrics I, University of Ulm, FRG</mods:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>†Section of Molecular Biology Department of Pediatrics I, University of Ulm</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Bartram, C R" sort="Bartram, C R" uniqKey="Bartram C" first="C. R." last="Bartram">C. R. Bartram</name>
<affiliation wicri:level="1"><mods:affiliation>†Section of Molecular Biology Department of Pediatrics I, University of Ulm, FRG</mods:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>†Section of Molecular Biology Department of Pediatrics I, University of Ulm</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Schmoll, H J" sort="Schmoll, H J" uniqKey="Schmoll H" first="H. J." last="Schmoll">H. J. Schmoll</name>
<affiliation wicri:level="1"><mods:affiliation>*Hämatologische Klinik, Medizinische Hochschule Hannover, University of Ulm, FRG</mods:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>*Hämatologische Klinik, Medizinische Hochschule Hannover, University of Ulm</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Bronson, D L" sort="Bronson, D L" uniqKey="Bronson D" first="D. L." last="Bronson">D. L. Bronson</name>
<affiliation wicri:level="1"><mods:affiliation>‡Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas, U.S.A.</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>‡Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:A6EB08728AA9B09FAB01BC30B98FB7BA26CEE854</idno>
<date when="1990" year="1990">1990</date>
<idno type="doi">10.1111/j.1365-2605.1990.tb01046.x</idno>
<idno type="url">https://api.istex.fr/ark:/67375/WNG-SSG9GLGB-8/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001448</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001448</idno>
<idno type="wicri:Area/Istex/Curation">001448</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main">Cellular oncogenes in human teratocarcinoma cell lines</title>
<author><name sortKey="Tesch, H" sort="Tesch, H" uniqKey="Tesch H" first="H." last="Tesch">H. Tesch</name>
<affiliation wicri:level="1"><mods:affiliation>Medizinische Klinik, Universität Köln, Köln, University of Ulm, FRG</mods:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Medizinische Klinik, Universität Köln, Köln, University of Ulm</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Correspondence address: Medizinische Klinik, Universität Köln, 5000 Köln 41, FRG</mods:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Correspondence address: Medizinische Klinik, Universität Köln, 5000 Köln 41</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Furba, R" sort="Furba, R" uniqKey="Furba R" first="R." last="Fürba">R. Fürba</name>
<affiliation wicri:level="1"><mods:affiliation>Medizinische Klinik, Universität Köln, Köln, University of Ulm, FRG</mods:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Medizinische Klinik, Universität Köln, Köln, University of Ulm</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Casper, J" sort="Casper, J" uniqKey="Casper J" first="J." last="Casper">J. Casper</name>
<affiliation wicri:level="1"><mods:affiliation>*Hämatologische Klinik, Medizinische Hochschule Hannover, University of Ulm, FRG</mods:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>*Hämatologische Klinik, Medizinische Hochschule Hannover, University of Ulm</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Lyons, J" sort="Lyons, J" uniqKey="Lyons J" first="J." last="Lyons">J. Lyons</name>
<affiliation wicri:level="1"><mods:affiliation>†Section of Molecular Biology Department of Pediatrics I, University of Ulm, FRG</mods:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>†Section of Molecular Biology Department of Pediatrics I, University of Ulm</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Bartram, C R" sort="Bartram, C R" uniqKey="Bartram C" first="C. R." last="Bartram">C. R. Bartram</name>
<affiliation wicri:level="1"><mods:affiliation>†Section of Molecular Biology Department of Pediatrics I, University of Ulm, FRG</mods:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>†Section of Molecular Biology Department of Pediatrics I, University of Ulm</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Schmoll, H J" sort="Schmoll, H J" uniqKey="Schmoll H" first="H. J." last="Schmoll">H. J. Schmoll</name>
<affiliation wicri:level="1"><mods:affiliation>*Hämatologische Klinik, Medizinische Hochschule Hannover, University of Ulm, FRG</mods:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>*Hämatologische Klinik, Medizinische Hochschule Hannover, University of Ulm</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Bronson, D L" sort="Bronson, D L" uniqKey="Bronson D" first="D. L." last="Bronson">D. L. Bronson</name>
<affiliation wicri:level="1"><mods:affiliation>‡Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas, U.S.A.</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>‡Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas</wicri:regionArea>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j" type="main">International Journal of Andrology</title>
<title level="j" type="alt">INTERNATIONAL JOURNAL OF ANDROLOGY</title>
<idno type="ISSN">0105-6263</idno>
<idno type="eISSN">1365-2605</idno>
<imprint><biblScope unit="vol">13</biblScope>
<biblScope unit="issue">5</biblScope>
<biblScope unit="page" from="377">377</biblScope>
<biblScope unit="page" to="388">388</biblScope>
<biblScope unit="page-count">12</biblScope>
<publisher>Blackwell Publishing Ltd</publisher>
<pubPlace>Oxford, UK</pubPlace>
<date type="published" when="1990-10">1990-10</date>
</imprint>
<idno type="ISSN">0105-6263</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0105-6263</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">We have analysed, by Northern blots, the expression of 14 cellular oncogenes in nine cell lines established from human teratocarcinomas. All lines expressed considerable amounts of p53, c‐Ki‐ras2, c‐Ha‐ras1, craf1, N‐myc, and c‐fos. Low level expression of c‐myc was detected in some lines. Southern blot experiments revealed no amplification or rearrangement of the c‐Ki‐ras2, N‐myc or c‐fos genes. Using a rapid dot‐blot screening procedure, based on a combination of in‐vitro amplification of ras‐specific sequences and oligonucleotide hybridization, we could detect no activation of Ha‐ras or Ki‐ras or any unexpressed N‐ras sequences secondary to a point mutation at codons 12, 13, or 61.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001448 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 001448 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Istex |étape= Curation |type= RBID |clé= ISTEX:A6EB08728AA9B09FAB01BC30B98FB7BA26CEE854 |texte= Cellular oncogenes in human teratocarcinoma cell lines }}
![]() | This area was generated with Dilib version V0.6.33. | ![]() |