Infectious eggplant mosaic tymovirus and ononis yellow mosaic tymovirus from cloned cDNA
Identifieur interne : 001C62 ( Istex/Checkpoint ); précédent : 001C61; suivant : 001C63Infectious eggplant mosaic tymovirus and ononis yellow mosaic tymovirus from cloned cDNA
Auteurs : M. L. Skotnicki [Australie] ; S. W. Ding [Australie] ; A. M. Mackenzie [Australie] ; A. J. Gibbs [Australie]Source :
- Archives of Virology [ 0304-8608 ] ; 1993-03-01.
English descriptors
- Teeft :
- Cdna, Cdna clone, Cdna synthesis, Clone, Diii, Dsdna, Eggplant, Eggplant mosaic tymovirus, Eggplant mosaic virus, Encoding, Endonuclease, First strand primer, Genome, Genomic, Glutinosa, Glutinosa seedlings, Host range determination, Host range differences, Hybrid, Hydrolysed, Infectious clones, Inoculated, Italic, Ligated, Linearized, Molecular basis, Mosaic, Mosaic tymovirus, Mosaic virus, Nonviral, Nonviral nucleotides, Nucleic, Nucleic acids, Nucleotide, Nucleotide sequence, Ononis, Oymv, Oymv genome, Pbluescript, Plants inoculated, Plasmid, Polymerase, Primer, Restriction endonucleases, Seedling, Skotnicki, Smaller plasmids, States biochemicals, Systemic symptoms, Terminus, Turnip, Tymovirus, Tymoviruses, Tymv, Vector hydrolysed, Viral, Viral genome, Viral genomes, Viral sequence, Viral terminus, Virion, Virion protein gene, Virion protein genes.
Abstract
Summary: Eggplant mosaic virus (EMV) and ononis yellow mosaic virus (OYMV) are two tymoviruses that have ssRNA genomes of about 6.2 kb and 6.3 kb, and which infect solanaceous and leguminous hosts, respectively. Full-length cDNA clones of these viruses were constructed with a T 7 promoter adjacent to the 5′ terminus of the DNA copy of the viral genome, and with unique restriction endonuclease sites at the 3′ terminus. This allowed RNA to be transcribed from the DNA encoding the genome. The transcript RNA was infectious when inoculated toNicotiana glutinosa (for EMV) andPisum sativum (for OYMV). These clones, together with clones of turnip yellow mosaic tymovirus, which infects brassicas, have been used to construct hybrids in which the virion protein gene was exchanged between EMV or OYMV and turnip yellow mosaic virus. These and other hybrids are being used to investigate the molecular basis for host range differences in tymoviruses.
Url:
DOI: 10.1007/BF01379079
Affiliations:
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ISTEX:2D30324D03D610AEEA4F7436DF6FB1A6BA7CFB57Le document en format XML
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<term>Dsdna</term>
<term>Eggplant</term>
<term>Eggplant mosaic tymovirus</term>
<term>Eggplant mosaic virus</term>
<term>Encoding</term>
<term>Endonuclease</term>
<term>First strand primer</term>
<term>Genome</term>
<term>Genomic</term>
<term>Glutinosa</term>
<term>Glutinosa seedlings</term>
<term>Host range determination</term>
<term>Host range differences</term>
<term>Hybrid</term>
<term>Hydrolysed</term>
<term>Infectious clones</term>
<term>Inoculated</term>
<term>Italic</term>
<term>Ligated</term>
<term>Linearized</term>
<term>Molecular basis</term>
<term>Mosaic</term>
<term>Mosaic tymovirus</term>
<term>Mosaic virus</term>
<term>Nonviral</term>
<term>Nonviral nucleotides</term>
<term>Nucleic</term>
<term>Nucleic acids</term>
<term>Nucleotide</term>
<term>Nucleotide sequence</term>
<term>Ononis</term>
<term>Oymv</term>
<term>Oymv genome</term>
<term>Pbluescript</term>
<term>Plants inoculated</term>
<term>Plasmid</term>
<term>Polymerase</term>
<term>Primer</term>
<term>Restriction endonucleases</term>
<term>Seedling</term>
<term>Skotnicki</term>
<term>Smaller plasmids</term>
<term>States biochemicals</term>
<term>Systemic symptoms</term>
<term>Terminus</term>
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<term>Tymovirus</term>
<term>Tymoviruses</term>
<term>Tymv</term>
<term>Vector hydrolysed</term>
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<term>Viral genome</term>
<term>Viral genomes</term>
<term>Viral sequence</term>
<term>Viral terminus</term>
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<term>Virion protein genes</term>
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<front><div type="abstract" xml:lang="en">Summary: Eggplant mosaic virus (EMV) and ononis yellow mosaic virus (OYMV) are two tymoviruses that have ssRNA genomes of about 6.2 kb and 6.3 kb, and which infect solanaceous and leguminous hosts, respectively. Full-length cDNA clones of these viruses were constructed with a T 7 promoter adjacent to the 5′ terminus of the DNA copy of the viral genome, and with unique restriction endonuclease sites at the 3′ terminus. This allowed RNA to be transcribed from the DNA encoding the genome. The transcript RNA was infectious when inoculated toNicotiana glutinosa (for EMV) andPisum sativum (for OYMV). These clones, together with clones of turnip yellow mosaic tymovirus, which infects brassicas, have been used to construct hybrids in which the virion protein gene was exchanged between EMV or OYMV and turnip yellow mosaic virus. These and other hybrids are being used to investigate the molecular basis for host range differences in tymoviruses.</div>
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