Generation of Cytomegalovirus (CMV)—Specific CD4 T Cell Lines Devoid of Alloreactivity, by Use of a Mixture of CMV—Phosphoprotein 65 Peptides for Reconstitution of the T Helper Repertoire
Identifieur interne : 000A49 ( Istex/Checkpoint ); précédent : 000A48; suivant : 000A50Generation of Cytomegalovirus (CMV)—Specific CD4 T Cell Lines Devoid of Alloreactivity, by Use of a Mixture of CMV—Phosphoprotein 65 Peptides for Reconstitution of the T Helper Repertoire
Auteurs : Giuseppina Li Pira ; Laura Bottone [Italie] ; Federico Ivaldi ; Augusto Tagliamacco ; Stefano Fiordoro ; Annamaria Ricciardi ; Giancarlo Barbano ; Fabrizio Manca [Italie]Source :
- Journal of Infectious Diseases [ 0022-1899 ] ; 2005.
Abstract
Background CD8 cells specific for cytomegalovirus (CMV) provide valuable support in immunocompromised patients. Recent studies have focused on the generation of cytotoxic T lymphocyte (CTL) lines by use of new biotechnological techniques. Yet CD4 cells have been neglected, even though they contribute to the persistence of adoptively transferred CTLs. Methods We identified novel T helper (Th) peptides recognized by CD4 cells on the immunodominant protein pp65. These peptides were used as a mixture to generate CD4 cell lines. Results The peptide library, which, theoretically, is recognized by 85% of white individuals on the basis of the frequency of the relevant human leukocyte antigen class II alleles, was stimulatory for 82% of pp65 responders. T cell lines generated by use of the pool recognized protein antigens. Selection for CMV-specific cells resulted in rapid depletion of allospecific T cells. Selection with individual peptides allowed further selection against potential cross-alloreactivity. Cultured CD4 cells showed no signs of functional senescence. CD4 cells activated by use of a Th peptide helped expand CMV-specific CTLs. Conclusions By use of a simple procedure, an immunodominant peptide library can generate T cell lines from allodonors, for the perspective reconstitution of the Th repertoire in immunocompromised hemopoietic stem-cell transplant recipients.
Url:
DOI: 10.1086/427040
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
ISTEX:8B827F167E2574AC11B1A52B11654654A19A1260Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Generation of Cytomegalovirus (CMV)—Specific CD4 T Cell Lines Devoid of Alloreactivity, by Use of a Mixture of CMV—Phosphoprotein 65 Peptides for Reconstitution of the T Helper Repertoire</title>
<author><name sortKey="Li Pira, Giuseppina" sort="Li Pira, Giuseppina" uniqKey="Li Pira G" first="Giuseppina" last="Li Pira">Giuseppina Li Pira</name>
</author>
<author><name sortKey="Bottone, Laura" sort="Bottone, Laura" uniqKey="Bottone L" first="Laura" last="Bottone">Laura Bottone</name>
</author>
<author><name sortKey="Ivaldi, Federico" sort="Ivaldi, Federico" uniqKey="Ivaldi F" first="Federico" last="Ivaldi">Federico Ivaldi</name>
</author>
<author><name sortKey="Tagliamacco, Augusto" sort="Tagliamacco, Augusto" uniqKey="Tagliamacco A" first="Augusto" last="Tagliamacco">Augusto Tagliamacco</name>
</author>
<author><name sortKey="Fiordoro, Stefano" sort="Fiordoro, Stefano" uniqKey="Fiordoro S" first="Stefano" last="Fiordoro">Stefano Fiordoro</name>
</author>
<author><name sortKey="Ricciardi, Annamaria" sort="Ricciardi, Annamaria" uniqKey="Ricciardi A" first="Annamaria" last="Ricciardi">Annamaria Ricciardi</name>
</author>
<author><name sortKey="Barbano, Giancarlo" sort="Barbano, Giancarlo" uniqKey="Barbano G" first="Giancarlo" last="Barbano">Giancarlo Barbano</name>
</author>
<author><name sortKey="Manca, Fabrizio" sort="Manca, Fabrizio" uniqKey="Manca F" first="Fabrizio" last="Manca">Fabrizio Manca</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:8B827F167E2574AC11B1A52B11654654A19A1260</idno>
<date when="2005" year="2005">2005</date>
<idno type="doi">10.1086/427040</idno>
<idno type="url">https://api.istex.fr/ark:/67375/HXZ-G74L1MMN-N/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000D78</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000D78</idno>
<idno type="wicri:Area/Istex/Curation">000D78</idno>
<idno type="wicri:Area/Istex/Checkpoint">000A49</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000A49</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main">Generation of Cytomegalovirus (CMV)—Specific CD4 T Cell Lines Devoid of Alloreactivity, by Use of a Mixture of CMV—Phosphoprotein 65 Peptides for Reconstitution of the T Helper Repertoire</title>
<author><name sortKey="Li Pira, Giuseppina" sort="Li Pira, Giuseppina" uniqKey="Li Pira G" first="Giuseppina" last="Li Pira">Giuseppina Li Pira</name>
<affiliation><wicri:noCountry code="subField">Genoa</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Bottone, Laura" sort="Bottone, Laura" uniqKey="Bottone L" first="Laura" last="Bottone">Laura Bottone</name>
<affiliation></affiliation>
<affiliation wicri:level="1"><country xml:lang="fr">Italie</country>
<wicri:regionArea>Department of Clinical and Biological Sciences, University of Insubria, Varese</wicri:regionArea>
<wicri:noRegion>Varese</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ivaldi, Federico" sort="Ivaldi, Federico" uniqKey="Ivaldi F" first="Federico" last="Ivaldi">Federico Ivaldi</name>
<affiliation><wicri:noCountry code="subField">Genoa</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Tagliamacco, Augusto" sort="Tagliamacco, Augusto" uniqKey="Tagliamacco A" first="Augusto" last="Tagliamacco">Augusto Tagliamacco</name>
<affiliation><wicri:noCountry code="subField">Genoa</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Fiordoro, Stefano" sort="Fiordoro, Stefano" uniqKey="Fiordoro S" first="Stefano" last="Fiordoro">Stefano Fiordoro</name>
<affiliation><wicri:noCountry code="subField">Genoa</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Ricciardi, Annamaria" sort="Ricciardi, Annamaria" uniqKey="Ricciardi A" first="Annamaria" last="Ricciardi">Annamaria Ricciardi</name>
<affiliation><wicri:noCountry code="subField">Genoa</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Barbano, Giancarlo" sort="Barbano, Giancarlo" uniqKey="Barbano G" first="Giancarlo" last="Barbano">Giancarlo Barbano</name>
<affiliation><wicri:noCountry code="subField">Genoa</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Manca, Fabrizio" sort="Manca, Fabrizio" uniqKey="Manca F" first="Fabrizio" last="Manca">Fabrizio Manca</name>
<affiliation></affiliation>
<affiliation wicri:level="1"><country wicri:rule="url">Italie</country>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j" type="main">Journal of Infectious Diseases</title>
<title level="j" type="abbrev">Journal of Infectious Diseases</title>
<idno type="ISSN">0022-1899</idno>
<idno type="eISSN">1537-6613</idno>
<imprint><publisher>The University of Chicago Press</publisher>
<date type="published">2005</date>
<biblScope unit="vol">191</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="215">215</biblScope>
<biblScope unit="page" to="226">226</biblScope>
</imprint>
<idno type="ISSN">0022-1899</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0022-1899</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract">Background CD8 cells specific for cytomegalovirus (CMV) provide valuable support in immunocompromised patients. Recent studies have focused on the generation of cytotoxic T lymphocyte (CTL) lines by use of new biotechnological techniques. Yet CD4 cells have been neglected, even though they contribute to the persistence of adoptively transferred CTLs. Methods We identified novel T helper (Th) peptides recognized by CD4 cells on the immunodominant protein pp65. These peptides were used as a mixture to generate CD4 cell lines. Results The peptide library, which, theoretically, is recognized by 85% of white individuals on the basis of the frequency of the relevant human leukocyte antigen class II alleles, was stimulatory for 82% of pp65 responders. T cell lines generated by use of the pool recognized protein antigens. Selection for CMV-specific cells resulted in rapid depletion of allospecific T cells. Selection with individual peptides allowed further selection against potential cross-alloreactivity. Cultured CD4 cells showed no signs of functional senescence. CD4 cells activated by use of a Th peptide helped expand CMV-specific CTLs. Conclusions By use of a simple procedure, an immunodominant peptide library can generate T cell lines from allodonors, for the perspective reconstitution of the Th repertoire in immunocompromised hemopoietic stem-cell transplant recipients.</div>
</front>
</TEI>
<affiliations><list><country><li>Italie</li>
</country>
</list>
<tree><noCountry><name sortKey="Barbano, Giancarlo" sort="Barbano, Giancarlo" uniqKey="Barbano G" first="Giancarlo" last="Barbano">Giancarlo Barbano</name>
<name sortKey="Fiordoro, Stefano" sort="Fiordoro, Stefano" uniqKey="Fiordoro S" first="Stefano" last="Fiordoro">Stefano Fiordoro</name>
<name sortKey="Ivaldi, Federico" sort="Ivaldi, Federico" uniqKey="Ivaldi F" first="Federico" last="Ivaldi">Federico Ivaldi</name>
<name sortKey="Li Pira, Giuseppina" sort="Li Pira, Giuseppina" uniqKey="Li Pira G" first="Giuseppina" last="Li Pira">Giuseppina Li Pira</name>
<name sortKey="Ricciardi, Annamaria" sort="Ricciardi, Annamaria" uniqKey="Ricciardi A" first="Annamaria" last="Ricciardi">Annamaria Ricciardi</name>
<name sortKey="Tagliamacco, Augusto" sort="Tagliamacco, Augusto" uniqKey="Tagliamacco A" first="Augusto" last="Tagliamacco">Augusto Tagliamacco</name>
</noCountry>
<country name="Italie"><noRegion><name sortKey="Bottone, Laura" sort="Bottone, Laura" uniqKey="Bottone L" first="Laura" last="Bottone">Laura Bottone</name>
</noRegion>
<name sortKey="Manca, Fabrizio" sort="Manca, Fabrizio" uniqKey="Manca F" first="Fabrizio" last="Manca">Fabrizio Manca</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000A49 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Checkpoint/biblio.hfd -nk 000A49 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Istex |étape= Checkpoint |type= RBID |clé= ISTEX:8B827F167E2574AC11B1A52B11654654A19A1260 |texte= Generation of Cytomegalovirus (CMV)—Specific CD4 T Cell Lines Devoid of Alloreactivity, by Use of a Mixture of CMV—Phosphoprotein 65 Peptides for Reconstitution of the T Helper Repertoire }}
![]() | This area was generated with Dilib version V0.6.33. | ![]() |