Identification of Lice Stored in Alcohol Using MALDI-TOF MS.
Identifieur interne : 000064 ( PubMed/Corpus ); précédent : 000063; suivant : 000065Identification of Lice Stored in Alcohol Using MALDI-TOF MS.
Auteurs : Hanene Benyahia ; Basma Ouarti ; Adama Zan Diarra ; Mehdi Boucheikhchoukh ; Mohamed Nadir Meguini ; Makhlouf Behidji ; Ahmed Benakhla ; Philippe Parola ; Lionel AlmerasSource :
- Journal of medical entomology [ 1938-2928 ] ; 2021.
Abstract
Lice pose major public and veterinary health problems with economic consequences. Their identification is essential and requires the development of an innovative strategy. MALDI-TOF MS has recently been proposed as a quick, inexpensive, and accurate tool for the identification of arthropods. Alcohol is one of the most frequently used storage methods and makes it possible to store samples for long periods at room temperature. Several recent studies have reported that alcohol alters protein profiles resulting from MS analysis. After preliminary studies on frozen lice, the purpose of this research was to evaluate the influence of alcohol preservation on the accuracy of lice identification by MALDI-TOF MS. To this end, lice stored in alcohol for variable periods were submitted for MS analysis and sample preparation protocols were optimized. The reproducibility and specificity of the MS spectra obtained on both these arthropod families allowed us to implement the reference MS spectra database (DB) with protein profiles of seven lice species stored in alcohol. Blind tests revealed a correct identification of 93.9% of Pediculus humanus corporis (Linnaeus, 1758) and 98.4% of the other lice species collected in the field. This study demonstrated that MALDI-TOF MS could be successfully used for the identification of lice stored in alcohol for different lengths of time.
DOI: 10.1093/jme/tjaa266
PubMed: 33346344
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last="Diarra">Adama Zan Diarra</name><affiliation><nlm:affiliation>Aix Marseille Univ, IRD, SSA, AP-HM, VITROME, Marseille, France.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>IHU-Méditerranée Infection, Marseille, France.</nlm:affiliation></affiliation></author><author><name sortKey="Boucheikhchoukh, Mehdi" sort="Boucheikhchoukh, Mehdi" uniqKey="Boucheikhchoukh M" first="Mehdi" last="Boucheikhchoukh">Mehdi Boucheikhchoukh</name><affiliation><nlm:affiliation>Department of Veterinary Sciences, Chadli Bendjedid University, El-Tarf, 36000, Algeria.</nlm:affiliation></affiliation></author><author><name sortKey="Meguini, Mohamed Nadir" sort="Meguini, Mohamed Nadir" uniqKey="Meguini M" first="Mohamed Nadir" last="Meguini">Mohamed Nadir Meguini</name><affiliation><nlm:affiliation>Institute of Veterinary and Agronomic Sciences, Mohamed Cherif Messaadia University, Souk-Ahras, Algeria.</nlm:affiliation></affiliation></author><author><name sortKey="Behidji, Makhlouf" sort="Behidji, Makhlouf" uniqKey="Behidji M" first="Makhlouf" last="Behidji">Makhlouf Behidji</name><affiliation><nlm:affiliation>Institute of Veterinary and Agronomic Sciences, Mohamed Cherif Messaadia University, Souk-Ahras, Algeria.</nlm:affiliation></affiliation></author><author><name sortKey="Benakhla, Ahmed" sort="Benakhla, Ahmed" uniqKey="Benakhla A" first="Ahmed" last="Benakhla">Ahmed Benakhla</name><affiliation><nlm:affiliation>Department of Veterinary Sciences, Chadli Bendjedid University, El-Tarf, 36000, Algeria.</nlm:affiliation></affiliation></author><author><name sortKey="Parola, Philippe" sort="Parola, Philippe" uniqKey="Parola P" first="Philippe" last="Parola">Philippe Parola</name><affiliation><nlm:affiliation>Aix Marseille Univ, IRD, SSA, AP-HM, VITROME, Marseille, France.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>IHU-Méditerranée Infection, Marseille, France.</nlm:affiliation></affiliation></author><author><name sortKey="Almeras, Lionel" sort="Almeras, Lionel" uniqKey="Almeras L" first="Lionel" last="Almeras">Lionel Almeras</name><affiliation><nlm:affiliation>Aix Marseille Univ, IRD, SSA, AP-HM, VITROME, Marseille, France.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>IHU-Méditerranée Infection, Marseille, France.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Unité Parasitologie et Entomologie, Département Microbiologie et Maladies Infectieuses, Institut de Recherche Biomédicale des Armées, 19-21 Boulevard Jean Moulin, 13005 Marseille, France.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2021">2021</date><idno type="RBID">pubmed:33346344</idno><idno type="pmid">33346344</idno><idno type="doi">10.1093/jme/tjaa266</idno><idno type="wicri:Area/PubMed/Corpus">000064</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000064</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Identification of Lice Stored in Alcohol Using MALDI-TOF MS.</title><author><name sortKey="Benyahia, Hanene" sort="Benyahia, Hanene" uniqKey="Benyahia H" first="Hanene" last="Benyahia">Hanene Benyahia</name><affiliation><nlm:affiliation>Aix Marseille Univ, IRD, SSA, AP-HM, VITROME, Marseille, France.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>IHU-Méditerranée Infection, Marseille, France.</nlm:affiliation></affiliation></author><author><name sortKey="Ouarti, Basma" sort="Ouarti, Basma" uniqKey="Ouarti B" first="Basma" last="Ouarti">Basma Ouarti</name><affiliation><nlm:affiliation>Aix Marseille Univ, IRD, SSA, AP-HM, VITROME, Marseille, France.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>IHU-Méditerranée Infection, Marseille, France.</nlm:affiliation></affiliation></author><author><name sortKey="Diarra, Adama Zan" sort="Diarra, Adama Zan" uniqKey="Diarra A" first="Adama Zan" last="Diarra">Adama Zan Diarra</name><affiliation><nlm:affiliation>Aix Marseille Univ, IRD, SSA, AP-HM, VITROME, Marseille, France.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>IHU-Méditerranée Infection, Marseille, France.</nlm:affiliation></affiliation></author><author><name sortKey="Boucheikhchoukh, Mehdi" sort="Boucheikhchoukh, Mehdi" uniqKey="Boucheikhchoukh M" first="Mehdi" last="Boucheikhchoukh">Mehdi Boucheikhchoukh</name><affiliation><nlm:affiliation>Department of Veterinary Sciences, Chadli Bendjedid University, El-Tarf, 36000, Algeria.</nlm:affiliation></affiliation></author><author><name sortKey="Meguini, Mohamed Nadir" sort="Meguini, Mohamed Nadir" uniqKey="Meguini M" first="Mohamed Nadir" last="Meguini">Mohamed Nadir Meguini</name><affiliation><nlm:affiliation>Institute of Veterinary and Agronomic Sciences, Mohamed Cherif Messaadia University, Souk-Ahras, Algeria.</nlm:affiliation></affiliation></author><author><name sortKey="Behidji, Makhlouf" sort="Behidji, Makhlouf" uniqKey="Behidji M" first="Makhlouf" last="Behidji">Makhlouf Behidji</name><affiliation><nlm:affiliation>Institute of Veterinary and Agronomic Sciences, Mohamed Cherif Messaadia University, Souk-Ahras, Algeria.</nlm:affiliation></affiliation></author><author><name sortKey="Benakhla, Ahmed" sort="Benakhla, Ahmed" uniqKey="Benakhla A" first="Ahmed" last="Benakhla">Ahmed Benakhla</name><affiliation><nlm:affiliation>Department of Veterinary Sciences, Chadli Bendjedid University, El-Tarf, 36000, Algeria.</nlm:affiliation></affiliation></author><author><name sortKey="Parola, Philippe" sort="Parola, Philippe" uniqKey="Parola P" first="Philippe" last="Parola">Philippe Parola</name><affiliation><nlm:affiliation>Aix Marseille Univ, IRD, SSA, AP-HM, VITROME, Marseille, France.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>IHU-Méditerranée Infection, Marseille, France.</nlm:affiliation></affiliation></author><author><name sortKey="Almeras, Lionel" sort="Almeras, Lionel" uniqKey="Almeras L" first="Lionel" last="Almeras">Lionel Almeras</name><affiliation><nlm:affiliation>Aix Marseille Univ, IRD, SSA, AP-HM, VITROME, Marseille, France.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>IHU-Méditerranée Infection, Marseille, France.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Unité Parasitologie et Entomologie, Département Microbiologie et Maladies Infectieuses, Institut de Recherche Biomédicale des Armées, 19-21 Boulevard Jean Moulin, 13005 Marseille, France.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Journal of medical entomology</title><idno type="eISSN">1938-2928</idno><imprint><date when="2021" type="published">2021</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Lice pose major public and veterinary health problems with economic consequences. Their identification is essential and requires the development of an innovative strategy. MALDI-TOF MS has recently been proposed as a quick, inexpensive, and accurate tool for the identification of arthropods. Alcohol is one of the most frequently used storage methods and makes it possible to store samples for long periods at room temperature. Several recent studies have reported that alcohol alters protein profiles resulting from MS analysis. After preliminary studies on frozen lice, the purpose of this research was to evaluate the influence of alcohol preservation on the accuracy of lice identification by MALDI-TOF MS. To this end, lice stored in alcohol for variable periods were submitted for MS analysis and sample preparation protocols were optimized. The reproducibility and specificity of the MS spectra obtained on both these arthropod families allowed us to implement the reference MS spectra database (DB) with protein profiles of seven lice species stored in alcohol. Blind tests revealed a correct identification of 93.9% of Pediculus humanus corporis (Linnaeus, 1758) and 98.4% of the other lice species collected in the field. This study demonstrated that MALDI-TOF MS could be successfully used for the identification of lice stored in alcohol for different lengths of time.</div></front></TEI><pubmed><MedlineCitation Status="In-Data-Review" Owner="NLM"><PMID Version="1">33346344</PMID><DateRevised><Year>2021</Year><Month>05</Month><Day>17</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1938-2928</ISSN><JournalIssue CitedMedium="Internet"><Volume>58</Volume><Issue>3</Issue><PubDate><Year>2021</Year><Month>May</Month><Day>15</Day></PubDate></JournalIssue><Title>Journal of medical entomology</Title><ISOAbbreviation>J Med Entomol</ISOAbbreviation></Journal><ArticleTitle>Identification of Lice Stored in Alcohol Using MALDI-TOF MS.</ArticleTitle><Pagination><MedlinePgn>1126-1133</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/jme/tjaa266</ELocationID><Abstract><AbstractText>Lice pose major public and veterinary health problems with economic consequences. Their identification is essential and requires the development of an innovative strategy. MALDI-TOF MS has recently been proposed as a quick, inexpensive, and accurate tool for the identification of arthropods. Alcohol is one of the most frequently used storage methods and makes it possible to store samples for long periods at room temperature. Several recent studies have reported that alcohol alters protein profiles resulting from MS analysis. After preliminary studies on frozen lice, the purpose of this research was to evaluate the influence of alcohol preservation on the accuracy of lice identification by MALDI-TOF MS. To this end, lice stored in alcohol for variable periods were submitted for MS analysis and sample preparation protocols were optimized. The reproducibility and specificity of the MS spectra obtained on both these arthropod families allowed us to implement the reference MS spectra database (DB) with protein profiles of seven lice species stored in alcohol. Blind tests revealed a correct identification of 93.9% of Pediculus humanus corporis (Linnaeus, 1758) and 98.4% of the other lice species collected in the field. This study demonstrated that MALDI-TOF MS could be successfully used for the identification of lice stored in alcohol for different lengths of time.</AbstractText><CopyrightInformation>© The Author(s) 2020. Published by Oxford University Press on behalf of Entomological Society of America.All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Benyahia</LastName><ForeName>Hanene</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Aix Marseille Univ, IRD, SSA, AP-HM, VITROME, Marseille, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>IHU-Méditerranée Infection, Marseille, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ouarti</LastName><ForeName>Basma</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Aix Marseille Univ, IRD, SSA, AP-HM, VITROME, Marseille, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>IHU-Méditerranée Infection, Marseille, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Diarra</LastName><ForeName>Adama Zan</ForeName><Initials>AZ</Initials><Identifier Source="ORCID">0000-0003-0490-5774</Identifier><AffiliationInfo><Affiliation>Aix Marseille Univ, IRD, SSA, AP-HM, VITROME, Marseille, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>IHU-Méditerranée Infection, Marseille, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Boucheikhchoukh</LastName><ForeName>Mehdi</ForeName><Initials>M</Initials><Identifier Source="ORCID">0000-0003-2741-9314</Identifier><AffiliationInfo><Affiliation>Department of Veterinary Sciences, Chadli Bendjedid University, El-Tarf, 36000, Algeria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Meguini</LastName><ForeName>Mohamed Nadir</ForeName><Initials>MN</Initials><AffiliationInfo><Affiliation>Institute of Veterinary and Agronomic Sciences, Mohamed Cherif Messaadia University, Souk-Ahras, Algeria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Behidji</LastName><ForeName>Makhlouf</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institute of Veterinary and Agronomic Sciences, Mohamed Cherif Messaadia University, Souk-Ahras, Algeria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Benakhla</LastName><ForeName>Ahmed</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Veterinary Sciences, Chadli Bendjedid University, El-Tarf, 36000, Algeria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Parola</LastName><ForeName>Philippe</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Aix Marseille Univ, IRD, SSA, AP-HM, VITROME, Marseille, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>IHU-Méditerranée Infection, Marseille, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Almeras</LastName><ForeName>Lionel</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Aix Marseille Univ, IRD, SSA, AP-HM, VITROME, Marseille, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>IHU-Méditerranée Infection, Marseille, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Unité Parasitologie et Entomologie, Département Microbiologie et Maladies Infectieuses, Institut de Recherche Biomédicale des Armées, 19-21 Boulevard Jean Moulin, 13005 Marseille, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Med Entomol</MedlineTA><NlmUniqueID>0375400</NlmUniqueID><ISSNLinking>0022-2585</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">MALDI-TOF MS</Keyword><Keyword MajorTopicYN="N">Poux</Keyword><Keyword MajorTopicYN="N">identification</Keyword><Keyword MajorTopicYN="N">lice</Keyword><Keyword MajorTopicYN="N">méthode de stockage</Keyword><Keyword MajorTopicYN="N">storage method</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>07</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>12</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>12</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>12</Month><Day>21</Day><Hour>8</Hour><Minute>46</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33346344</ArticleId><ArticleId IdType="pii">6042599</ArticleId><ArticleId IdType="doi">10.1093/jme/tjaa266</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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