A technique for microfilarial detection in preserved blood using nuclepore filters.
Identifieur interne : 005C54 ( PubMed/Curation ); précédent : 005C53; suivant : 005C55A technique for microfilarial detection in preserved blood using nuclepore filters.
Auteurs : J W Dickerson [États-Unis] ; M L Eberhard ; P J LammieSource :
- The Journal of parasitology [ 0022-3395 ] ; 1990.
Descripteurs français
- KwdFr :
- MESH :
- isolement et purification : Microfilaria, Wuchereria bancrofti.
- parasitologie : Filariose lymphatique.
- sang : Filariose lymphatique.
- Acide citrique, Acide édétique, Alcools gras, Animaux, Citrates, Conservation de sang, Filtration, Formaldéhyde, Humains, Tensioactifs.
English descriptors
- KwdEn :
- Animals, Blood Preservation, Citrates, Citric Acid, Edetic Acid, Elephantiasis, Filarial (blood), Elephantiasis, Filarial (parasitology), Fatty Alcohols, Filtration, Formaldehyde, Humans, Microfilariae (isolation & purification), Surface-Active Agents, Wuchereria bancrofti (isolation & purification).
- MESH :
- chemical : Citrates, Citric Acid, Edetic Acid, Fatty Alcohols, Formaldehyde, Surface-Active Agents.
- blood : Elephantiasis, Filarial.
- isolation & purification : Microfilariae, Wuchereria bancrofti.
- parasitology : Elephantiasis, Filarial.
- Animals, Blood Preservation, Filtration, Humans.
Abstract
Nuclepore filtration is now the most widely used method of detecting microfilaremia, particularly if microfilariae are few in number. However, this system requires the blood sample to be processed promptly after collection. Using blood from Wuchereria bancrofti-infected patients, 3 solutions were tested to determine whether blood could be held for delayed processing. Of these, we identified 1, 2% formalin/10% Teepol, in which microfilaremic blood can be held for at least 9 mo without deterioration of microfilarial structure or decrease in microfilarial numbers. In addition, this mixture passed easily through a 5-microns filter at all times tested. Examination of more than 300 blood samples held in 2% formalin/10% Teepol showed that this solution can utilize the convenience and sensitivity of membrane filtration while eliminating the need to perform testing immediately after the blood is collected.
PubMed: 2123924
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xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Parasitic Diseases Branch, Centers for Disease Control, U.S. Department of Health and Human Services, Atlanta</wicri:cityArea></affiliation></author><author><name sortKey="Eberhard, M L" sort="Eberhard, M L" uniqKey="Eberhard M" first="M L" last="Eberhard">M L Eberhard</name></author><author><name sortKey="Lammie, P J" sort="Lammie, P J" uniqKey="Lammie P" first="P J" last="Lammie">P J Lammie</name></author></analytic><series><title level="j">The Journal of parasitology</title><idno type="ISSN">0022-3395</idno><imprint><date when="1990" type="published">1990</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Blood Preservation</term><term>Citrates</term><term>Citric Acid</term><term>Edetic Acid</term><term>Elephantiasis, Filarial (blood)</term><term>Elephantiasis, Filarial (parasitology)</term><term>Fatty Alcohols</term><term>Filtration</term><term>Formaldehyde</term><term>Humans</term><term>Microfilariae (isolation & purification)</term><term>Surface-Active Agents</term><term>Wuchereria bancrofti (isolation & purification)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acide citrique</term><term>Acide édétique</term><term>Alcools gras</term><term>Animaux</term><term>Citrates</term><term>Conservation de sang</term><term>Filariose lymphatique (parasitologie)</term><term>Filariose lymphatique (sang)</term><term>Filtration</term><term>Formaldéhyde</term><term>Humains</term><term>Microfilaria (isolement et purification)</term><term>Tensioactifs</term><term>Wuchereria bancrofti (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Citrates</term><term>Citric Acid</term><term>Edetic Acid</term><term>Fatty Alcohols</term><term>Formaldehyde</term><term>Surface-Active Agents</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Microfilariae</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Microfilaria</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Blood Preservation</term><term>Filtration</term><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Acide citrique</term><term>Acide édétique</term><term>Alcools gras</term><term>Animaux</term><term>Citrates</term><term>Conservation de sang</term><term>Filtration</term><term>Formaldéhyde</term><term>Humains</term><term>Tensioactifs</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Nuclepore filtration is now the most widely used method of detecting microfilaremia, particularly if microfilariae are few in number. However, this system requires the blood sample to be processed promptly after collection. Using blood from Wuchereria bancrofti-infected patients, 3 solutions were tested to determine whether blood could be held for delayed processing. Of these, we identified 1, 2% formalin/10% Teepol, in which microfilaremic blood can be held for at least 9 mo without deterioration of microfilarial structure or decrease in microfilarial numbers. In addition, this mixture passed easily through a 5-microns filter at all times tested. Examination of more than 300 blood samples held in 2% formalin/10% Teepol showed that this solution can utilize the convenience and sensitivity of membrane filtration while eliminating the need to perform testing immediately after the blood is collected.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">2123924</PMID><DateCreated><Year>1991</Year><Month>01</Month><Day>24</Day></DateCreated><DateCompleted><Year>1991</Year><Month>01</Month><Day>24</Day></DateCompleted><DateRevised><Year>2016</Year><Month>11</Month><Day>23</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-3395</ISSN><JournalIssue CitedMedium="Print"><Volume>76</Volume><Issue>6</Issue><PubDate><Year>1990</Year><Month>Dec</Month></PubDate></JournalIssue><Title>The Journal of parasitology</Title><ISOAbbreviation>J. Parasitol.</ISOAbbreviation></Journal><ArticleTitle>A technique for microfilarial detection in preserved blood using nuclepore filters.</ArticleTitle><Pagination><MedlinePgn>829-33</MedlinePgn></Pagination><Abstract><AbstractText>Nuclepore filtration is now the most widely used method of detecting microfilaremia, particularly if microfilariae are few in number. However, this system requires the blood sample to be processed promptly after collection. Using blood from Wuchereria bancrofti-infected patients, 3 solutions were tested to determine whether blood could be held for delayed processing. Of these, we identified 1, 2% formalin/10% Teepol, in which microfilaremic blood can be held for at least 9 mo without deterioration of microfilarial structure or decrease in microfilarial numbers. In addition, this mixture passed easily through a 5-microns filter at all times tested. Examination of more than 300 blood samples held in 2% formalin/10% Teepol showed that this solution can utilize the convenience and sensitivity of membrane filtration while eliminating the need to perform testing immediately after the blood is collected.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Dickerson</LastName><ForeName>J W</ForeName><Initials>JW</Initials><AffiliationInfo><Affiliation>Parasitic Diseases Branch, Centers for Disease Control, U.S. Department of Health and Human Services, Atlanta, Georgia 30333.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Eberhard</LastName><ForeName>M L</ForeName><Initials>ML</Initials></Author><Author ValidYN="Y"><LastName>Lammie</LastName><ForeName>P J</ForeName><Initials>PJ</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>AI 16315</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013487">Research Support, U.S. Gov't, P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Parasitol</MedlineTA><NlmUniqueID>7803124</NlmUniqueID><ISSNLinking>0022-3395</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002951">Citrates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005233">Fatty Alcohols</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013501">Surface-Active Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>1HG84L3525</RegistryNumber><NameOfSubstance UI="D005557">Formaldehyde</NameOfSubstance></Chemical><Chemical><RegistryNumber>2968PHW8QP</RegistryNumber><NameOfSubstance UI="D019343">Citric Acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>65900-34-7</RegistryNumber><NameOfSubstance UI="C003381">teepol</NameOfSubstance></Chemical><Chemical><RegistryNumber>9G34HU7RV0</RegistryNumber><NameOfSubstance UI="D004492">Edetic Acid</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001793" MajorTopicYN="Y">Blood Preservation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002951" MajorTopicYN="N">Citrates</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019343" MajorTopicYN="N">Citric Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004492" MajorTopicYN="N">Edetic Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004605" MajorTopicYN="N">Elephantiasis, Filarial</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005233" MajorTopicYN="N">Fatty Alcohols</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005374" MajorTopicYN="N">Filtration</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005557" MajorTopicYN="N">Formaldehyde</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008842" MajorTopicYN="N">Microfilariae</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013501" MajorTopicYN="N">Surface-Active Agents</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014958" MajorTopicYN="N">Wuchereria bancrofti</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1990</Year><Month>12</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1990</Year><Month>12</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1990</Year><Month>12</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">2123924</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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