Construction of a recombinant plasmid harbouring the glyceraldenyde-3-phosphate dehydrogenase gene of periodic Brugia malayi and observation on DNA immunity.
Identifieur interne : 002101 ( PubMed/Corpus ); précédent : 002100; suivant : 002102Construction of a recombinant plasmid harbouring the glyceraldenyde-3-phosphate dehydrogenase gene of periodic Brugia malayi and observation on DNA immunity.
Auteurs : Z. Fang ; H. Tong ; S. Zhang ; H. Fang ; S. Lu ; B. XuSource :
- Indian journal of medical microbiology [ 1998-3646 ]
English descriptors
- KwdEn :
- Adjuvants, Immunologic (administration & dosage), Animals, Antibodies, Helminth (blood), Brugia malayi (enzymology), Brugia malayi (genetics), Brugia malayi (immunology), Cell Proliferation, Elephantiasis, Filarial (immunology), Elephantiasis, Filarial (prevention & control), Enzyme-Linked Immunosorbent Assay, Female, Glyceraldehyde-3-Phosphate Dehydrogenases (genetics), Glyceraldehyde-3-Phosphate Dehydrogenases (immunology), Injections, Intramuscular, Mice, Mice, Inbred BALB C, Oligodeoxyribonucleotides (administration & dosage), Plasmids (administration & dosage), Spleen (immunology), T-Lymphocytes (immunology), Vaccination (methods), Vaccines, DNA (administration & dosage), Vaccines, DNA (genetics), Vaccines, DNA (immunology), Vaccines, Synthetic (administration & dosage), Vaccines, Synthetic (genetics), Vaccines, Synthetic (immunology).
- MESH :
- chemical , administration & dosage : Adjuvants, Immunologic, Oligodeoxyribonucleotides, Vaccines, DNA, Vaccines, Synthetic.
- chemical , blood : Antibodies, Helminth.
- administration & dosage : Plasmids.
- enzymology : Brugia malayi.
- genetics : Brugia malayi, Glyceraldehyde-3-Phosphate Dehydrogenases, Vaccines, DNA, Vaccines, Synthetic.
- immunology : Brugia malayi, Elephantiasis, Filarial, Glyceraldehyde-3-Phosphate Dehydrogenases, Spleen, T-Lymphocytes, Vaccines, DNA, Vaccines, Synthetic.
- methods : Vaccination.
- prevention & control : Elephantiasis, Filarial.
- Animals, Cell Proliferation, Enzyme-Linked Immunosorbent Assay, Female, Injections, Intramuscular, Mice, Mice, Inbred BALB C.
Abstract
Controlling and eliminating lymphatic filariasis will require further research of preventative measures and implementation. Parasite is dependent on glycolysis for ATP production. The glycolytic enzyme glyceraldenyde-3-phosphate dehydrogenase (GAPDH) plays an important role in glycolysis and therefore is either a potential target for anti-parasite drug development or a vaccine candidate. Therefore, we tried to investigate the DNA vaccine-elicited immune responses in BALB/c mice.
DOI: 10.4103/0255-0857.96691
PubMed: 22664436
Links to Exploration step
pubmed:22664436Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Construction of a recombinant plasmid harbouring the glyceraldenyde-3-phosphate dehydrogenase gene of periodic Brugia malayi and observation on DNA immunity.</title><author><name sortKey="Fang, Z" sort="Fang, Z" uniqKey="Fang Z" first="Z" last="Fang">Z. Fang</name><affiliation><nlm:affiliation>Department of Parasitology, School of Basic Medical Sciences, Nantong University, Nantong, Jiangsu - 226 001, China.</nlm:affiliation></affiliation></author><author><name sortKey="Tong, H" sort="Tong, H" uniqKey="Tong H" first="H" last="Tong">H. Tong</name></author><author><name sortKey="Zhang, S" sort="Zhang, S" uniqKey="Zhang S" first="S" last="Zhang">S. Zhang</name></author><author><name sortKey="Fang, H" sort="Fang, H" uniqKey="Fang H" first="H" last="Fang">H. Fang</name></author><author><name sortKey="Lu, S" sort="Lu, S" uniqKey="Lu S" first="S" last="Lu">S. Lu</name></author><author><name sortKey="Xu, B" sort="Xu, B" uniqKey="Xu B" first="B" last="Xu">B. Xu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="????"><PubDate><MedlineDate>2012 Apr-Jun</MedlineDate></PubDate></date><idno type="RBID">pubmed:22664436</idno><idno type="pmid">22664436</idno><idno type="doi">10.4103/0255-0857.96691</idno><idno type="wicri:Area/PubMed/Corpus">002101</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002101</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Construction of a recombinant plasmid harbouring the glyceraldenyde-3-phosphate dehydrogenase gene of periodic Brugia malayi and observation on DNA immunity.</title><author><name sortKey="Fang, Z" sort="Fang, Z" uniqKey="Fang Z" first="Z" last="Fang">Z. Fang</name><affiliation><nlm:affiliation>Department of Parasitology, School of Basic Medical Sciences, Nantong University, Nantong, Jiangsu - 226 001, China.</nlm:affiliation></affiliation></author><author><name sortKey="Tong, H" sort="Tong, H" uniqKey="Tong H" first="H" last="Tong">H. Tong</name></author><author><name sortKey="Zhang, S" sort="Zhang, S" uniqKey="Zhang S" first="S" last="Zhang">S. Zhang</name></author><author><name sortKey="Fang, H" sort="Fang, H" uniqKey="Fang H" first="H" last="Fang">H. Fang</name></author><author><name sortKey="Lu, S" sort="Lu, S" uniqKey="Lu S" first="S" last="Lu">S. Lu</name></author><author><name sortKey="Xu, B" sort="Xu, B" uniqKey="Xu B" first="B" last="Xu">B. Xu</name></author></analytic><series><title level="j">Indian journal of medical microbiology</title><idno type="eISSN">1998-3646</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adjuvants, Immunologic (administration & dosage)</term><term>Animals</term><term>Antibodies, Helminth (blood)</term><term>Brugia malayi (enzymology)</term><term>Brugia malayi (genetics)</term><term>Brugia malayi (immunology)</term><term>Cell Proliferation</term><term>Elephantiasis, Filarial (immunology)</term><term>Elephantiasis, Filarial (prevention & control)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Female</term><term>Glyceraldehyde-3-Phosphate Dehydrogenases (genetics)</term><term>Glyceraldehyde-3-Phosphate Dehydrogenases (immunology)</term><term>Injections, Intramuscular</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Oligodeoxyribonucleotides (administration & dosage)</term><term>Plasmids (administration & dosage)</term><term>Spleen (immunology)</term><term>T-Lymphocytes (immunology)</term><term>Vaccination (methods)</term><term>Vaccines, DNA (administration & dosage)</term><term>Vaccines, DNA (genetics)</term><term>Vaccines, DNA (immunology)</term><term>Vaccines, Synthetic (administration & dosage)</term><term>Vaccines, Synthetic (genetics)</term><term>Vaccines, Synthetic (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Adjuvants, Immunologic</term><term>Oligodeoxyribonucleotides</term><term>Vaccines, DNA</term><term>Vaccines, Synthetic</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Helminth</term></keywords><keywords scheme="MESH" qualifier="administration & dosage" xml:lang="en"><term>Plasmids</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Brugia malayi</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Brugia malayi</term><term>Glyceraldehyde-3-Phosphate Dehydrogenases</term><term>Vaccines, DNA</term><term>Vaccines, Synthetic</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Brugia malayi</term><term>Elephantiasis, Filarial</term><term>Glyceraldehyde-3-Phosphate Dehydrogenases</term><term>Spleen</term><term>T-Lymphocytes</term><term>Vaccines, DNA</term><term>Vaccines, Synthetic</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Vaccination</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Proliferation</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Female</term><term>Injections, Intramuscular</term><term>Mice</term><term>Mice, Inbred BALB C</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Controlling and eliminating lymphatic filariasis will require further research of preventative measures and implementation. Parasite is dependent on glycolysis for ATP production. The glycolytic enzyme glyceraldenyde-3-phosphate dehydrogenase (GAPDH) plays an important role in glycolysis and therefore is either a potential target for anti-parasite drug development or a vaccine candidate. Therefore, we tried to investigate the DNA vaccine-elicited immune responses in BALB/c mice.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22664436</PMID><DateCreated><Year>2012</Year><Month>06</Month><Day>05</Day></DateCreated><DateCompleted><Year>2012</Year><Month>09</Month><Day>28</Day></DateCompleted><DateRevised><Year>2012</Year><Month>06</Month><Day>05</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1998-3646</ISSN><JournalIssue CitedMedium="Internet"><Volume>30</Volume><Issue>2</Issue><PubDate><MedlineDate>2012 Apr-Jun</MedlineDate></PubDate></JournalIssue><Title>Indian journal of medical microbiology</Title><ISOAbbreviation>Indian J Med Microbiol</ISOAbbreviation></Journal><ArticleTitle>Construction of a recombinant plasmid harbouring the glyceraldenyde-3-phosphate dehydrogenase gene of periodic Brugia malayi and observation on DNA immunity.</ArticleTitle><Pagination><MedlinePgn>193-7</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.4103/0255-0857.96691</ELocationID><Abstract><AbstractText Label="PURPOSE" NlmCategory="OBJECTIVE">Controlling and eliminating lymphatic filariasis will require further research of preventative measures and implementation. Parasite is dependent on glycolysis for ATP production. The glycolytic enzyme glyceraldenyde-3-phosphate dehydrogenase (GAPDH) plays an important role in glycolysis and therefore is either a potential target for anti-parasite drug development or a vaccine candidate. Therefore, we tried to investigate the DNA vaccine-elicited immune responses in BALB/c mice.</AbstractText><AbstractText Label="MATERIALS AND METHODS" NlmCategory="METHODS">We cloned a gene encoding the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from periodic Brugia malayi into vector pcDNA3.1. Mice were injected at a dosage of 100 μg recombinant plasmid DNA with CpG intramuscular injection and immunized three times at 2-week intervals. pcDNA3.1 and normal saline were used as control. The tissue of muscles at the 4 weeks after the third injection was collected and target genes were detected using RT-PCR. The humoral responses elicited in mice by inoculation with the recombinant plasmid pcDNA3.1-BmGAPDH were detected using a standard ELISA. Two weeks after the third immunization, stimulation index (SI) was measured using the MTT method and the level of secreted IL-4 and INF-g were detected using ELISA.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Specific gene fragment coding GAPDH was amplified and the recombinant plasmid pcDNA3.1-BmGAPDH was constructed. Post-challenge sera from the mice immunized with the DNA vaccine had specific antibody titres of 1:1600 to 1:6400, and the highest titre was observed in the mice that were inoculated by pcDNA3.1-BmGAPDH/CpG at 6 weeks. At 4 weeks after immunization, the spleens of the mice were obviously enlarged. The proliferation of spleen T lymphocytes seen on the MTT assay was higher in the pcDNA3.1-BmGAPDH group than in the control group (P value <0.05). The levels of IL-4 and INF-γ in serums from the immunized mice were significantly higher than that of the control (P value <0.05).</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">We conclude that the recombinant eukaryotic plasmid pcDNA3.1-BmGAPDH could elicit humoral and cellular immune responses in mice.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Fang</LastName><ForeName>Z</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Department of Parasitology, School of Basic Medical Sciences, Nantong University, Nantong, Jiangsu - 226 001, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tong</LastName><ForeName>H</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Fang</LastName><ForeName>H</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>B</ForeName><Initials>B</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>India</Country><MedlineTA>Indian J Med Microbiol</MedlineTA><NlmUniqueID>8700903</NlmUniqueID><ISSNLinking>0255-0857</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000276">Adjuvants, Immunologic</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000909">Antibodies, Helminth</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C408982">CPG-oligonucleotide</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009838">Oligodeoxyribonucleotides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019444">Vaccines, DNA</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014614">Vaccines, Synthetic</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.2.1.-</RegistryNumber><NameOfSubstance UI="D005987">Glyceraldehyde-3-Phosphate Dehydrogenases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000276" MajorTopicYN="N">Adjuvants, Immunologic</DescriptorName><QualifierName UI="Q000008" MajorTopicYN="N">administration & dosage</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000909" MajorTopicYN="N">Antibodies, Helminth</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017178" MajorTopicYN="N">Brugia malayi</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D049109" MajorTopicYN="N">Cell Proliferation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004605" MajorTopicYN="N">Elephantiasis, Filarial</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000517" MajorTopicYN="N">prevention & control</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005987" MajorTopicYN="N">Glyceraldehyde-3-Phosphate Dehydrogenases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007273" MajorTopicYN="N">Injections, Intramuscular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008807" MajorTopicYN="N">Mice, Inbred BALB C</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009838" MajorTopicYN="N">Oligodeoxyribonucleotides</DescriptorName><QualifierName UI="Q000008" MajorTopicYN="N">administration & dosage</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010957" MajorTopicYN="N">Plasmids</DescriptorName><QualifierName UI="Q000008" MajorTopicYN="N">administration & dosage</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013154" MajorTopicYN="N">Spleen</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013601" MajorTopicYN="N">T-Lymphocytes</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014611" MajorTopicYN="N">Vaccination</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019444" MajorTopicYN="N">Vaccines, DNA</DescriptorName><QualifierName UI="Q000008" MajorTopicYN="Y">administration & dosage</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014614" MajorTopicYN="N">Vaccines, Synthetic</DescriptorName><QualifierName UI="Q000008" MajorTopicYN="N">administration & dosage</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>6</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>6</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>9</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22664436</ArticleId><ArticleId IdType="pii">IndianJMedMicrobiol_2012_30_2_193_96691</ArticleId><ArticleId IdType="doi">10.4103/0255-0857.96691</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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