Epidemiological Screening of Lymphatic Filariasis Among Immigrants Using Dipstick Colloidal Dye Immunoassay
Identifieur interne : 005041 ( Ncbi/Merge ); précédent : 005040; suivant : 005042Epidemiological Screening of Lymphatic Filariasis Among Immigrants Using Dipstick Colloidal Dye Immunoassay
Auteurs : A. Wan Omar ; O. Sulaiman [Malaisie] ; S. Yusof [Malaisie] ; G. Ismail [Malaisie] ; M. S Fatmah [Malaisie] ; N. Rahmah [Malaisie] ; A. A. Khairul [Malaisie]Source :
- The Malaysian Journal of Medical Sciences : MJMS [ 1394-195X ] ; 2001.
Abstract
We have recently reported that a dipstick colloidal dye immunoassay (DIA) that detect parasite antigens in human serum is sensitive and specific for the diagnosis of active infection of lymphatic filariasis. Rabbit polyclonal antibodies (RbBmCAg) labelled with a commercial dye, palanil navy blue was used to detect filarial antigenemia among Indonesian and Bangladeshi immigrant workers (N= 630) at oil palm estates at Hulu Trengganu District, Peninsular Malaysia. Microfilaremia with
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PubMed: 22893756
PubMed Central: 3413645
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<author><name sortKey="Sulaiman, O" sort="Sulaiman, O" uniqKey="Sulaiman O" first="O" last="Sulaiman">O. Sulaiman</name>
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<author><name sortKey="Yusof, S" sort="Yusof, S" uniqKey="Yusof S" first="S" last="Yusof">S. Yusof</name>
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<series><title level="j">The Malaysian Journal of Medical Sciences : MJMS</title>
<idno type="ISSN">1394-195X</idno>
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<front><div type="abstract" xml:lang="en"><p>We have recently reported that a dipstick colloidal dye immunoassay (DIA) that detect parasite antigens in human serum is sensitive and specific for the diagnosis of active infection of lymphatic filariasis. Rabbit polyclonal antibodies (RbBmCAg) labelled with a commercial dye, palanil navy blue was used to detect filarial antigenemia among Indonesian and Bangladeshi immigrant workers (N= 630) at oil palm estates at Hulu Trengganu District, Peninsular Malaysia. Microfilaremia with <italic>Brugia malayi</italic>
were detected in 51 (8.10 %) individuals, of which 42 (6.67 %) were among the Indonesians and 9 (1.98 %) among the Bangladeshis. Microfilaremia with <italic>Wuchereria bancrofti</italic>
were detected in 33 (5.24 %) individuals of which 15 (2.38 %) were among the Indonesians and 18 (2.86 %) among the Bangladeshis workers. The DIA detected 96 (15.24 %) antigenemic cases which comprise of all the microfilaremic cases and 15 (2.38 %) amicrofilaremic cases. The amicrofilaremic cases with filarial antigenemia consisted of 9 (1. 43 %) Indonesians and 6 (0.95%) Bangladeshis. We have used 6 ul of the RbBmCAg and diluted (1:10) patients’ sera per dipstick which make the DIA reagent conservative. The DIA is a rapid test and can be read in approximate 2 hours.. Additionally, coloured dots developed in the DIA can be qualitatively assessed visually for intensity. The DIA does not require sophisticated equipment or radioactivity, and therefore suitable for field application.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Malays J Med Sci</journal-id>
<journal-id journal-id-type="iso-abbrev">Malays J Med Sci</journal-id>
<journal-id journal-id-type="publisher-id">Malaysian Journal of Medical Sciences</journal-id>
<journal-title-group><journal-title>The Malaysian Journal of Medical Sciences : MJMS</journal-title>
</journal-title-group>
<issn pub-type="ppub">1394-195X</issn>
<issn pub-type="epub">2180-4303</issn>
<publisher><publisher-name>Penerbit Universiti Sains Malaysia</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">22893756</article-id>
<article-id pub-id-type="pmc">3413645</article-id>
<article-id pub-id-type="publisher-id">mjms-8-2-019</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Epidemiological Screening of Lymphatic Filariasis Among Immigrants Using Dipstick Colloidal Dye Immunoassay</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Wan Omar</surname>
<given-names>A</given-names>
</name>
<xref ref-type="corresp" rid="c1-mjms-8-2-019"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Sulaiman</surname>
<given-names>O</given-names>
</name>
<xref ref-type="aff" rid="af2-mjms-8-2-019"><sup>*</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Yusof</surname>
<given-names>S</given-names>
</name>
<xref ref-type="aff" rid="af2-mjms-8-2-019"><sup>*</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ismail</surname>
<given-names>G</given-names>
</name>
<xref ref-type="aff" rid="af2-mjms-8-2-019"><sup>*</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Fatmah</surname>
<given-names>M.S</given-names>
</name>
<xref ref-type="aff" rid="af2-mjms-8-2-019"><sup>*</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Rahmah</surname>
<given-names>N</given-names>
</name>
<xref ref-type="aff" rid="af3-mjms-8-2-019"><sup>**</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Khairul</surname>
<given-names>A.A.</given-names>
</name>
<xref ref-type="aff" rid="af4-mjms-8-2-019"><sup>***</sup>
</xref>
</contrib>
<aff id="af1-mjms-8-2-019">Department of Clinical Laboratory Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia</aff>
</contrib-group>
<aff id="af2-mjms-8-2-019"><label>*</label>
Department of Parasitology and Medical Entomology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia</aff>
<aff id="af3-mjms-8-2-019"><label>**</label>
Deparment of Microbiology and Parasitology, School of Medical Sciences, School of Medical Sciences, Universiti Sains Malaysia 16150 Kubang Kerian, Kelantan, Malaysia</aff>
<aff id="af4-mjms-8-2-019"><label>***</label>
Department of Parasitology, Faculty of Medicine, Universiti Malaya, 50603 Kuala Lumpur, Malaysia</aff>
<author-notes><corresp id="c1-mjms-8-2-019">Correspondence : Professor Wan Omar Abdullah, Department of Clinical Laboratory Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia</corresp>
</author-notes>
<pub-date pub-type="ppub"><month>7</month>
<year>2001</year>
</pub-date>
<volume>8</volume>
<issue>2</issue>
<fpage>19</fpage>
<lpage>24</lpage>
<history><date date-type="received"><day>12</day>
<month>6</month>
<year>2000</year>
</date>
<date date-type="rev-recd"><day>04</day>
<month>1</month>
<year>2001</year>
</date>
<date date-type="accepted"><day>15</day>
<month>5</month>
<year>2001</year>
</date>
</history>
<permissions><copyright-statement>© Penerbit Universiti Sains Malaysia, 2001</copyright-statement>
<copyright-year>2001</copyright-year>
</permissions>
<abstract><p>We have recently reported that a dipstick colloidal dye immunoassay (DIA) that detect parasite antigens in human serum is sensitive and specific for the diagnosis of active infection of lymphatic filariasis. Rabbit polyclonal antibodies (RbBmCAg) labelled with a commercial dye, palanil navy blue was used to detect filarial antigenemia among Indonesian and Bangladeshi immigrant workers (N= 630) at oil palm estates at Hulu Trengganu District, Peninsular Malaysia. Microfilaremia with <italic>Brugia malayi</italic>
were detected in 51 (8.10 %) individuals, of which 42 (6.67 %) were among the Indonesians and 9 (1.98 %) among the Bangladeshis. Microfilaremia with <italic>Wuchereria bancrofti</italic>
were detected in 33 (5.24 %) individuals of which 15 (2.38 %) were among the Indonesians and 18 (2.86 %) among the Bangladeshis workers. The DIA detected 96 (15.24 %) antigenemic cases which comprise of all the microfilaremic cases and 15 (2.38 %) amicrofilaremic cases. The amicrofilaremic cases with filarial antigenemia consisted of 9 (1. 43 %) Indonesians and 6 (0.95%) Bangladeshis. We have used 6 ul of the RbBmCAg and diluted (1:10) patients’ sera per dipstick which make the DIA reagent conservative. The DIA is a rapid test and can be read in approximate 2 hours.. Additionally, coloured dots developed in the DIA can be qualitatively assessed visually for intensity. The DIA does not require sophisticated equipment or radioactivity, and therefore suitable for field application.</p>
</abstract>
<kwd-group><kwd>Dipstick</kwd>
<kwd>Colloidal Dye</kwd>
<kwd>Immunoassay</kwd>
<kwd>Filariasis</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Malaisie</li>
</country>
</list>
<tree><noCountry><name sortKey="Wan Omar, A" sort="Wan Omar, A" uniqKey="Wan Omar A" first="A" last="Wan Omar">A. Wan Omar</name>
</noCountry>
<country name="Malaisie"><noRegion><name sortKey="Sulaiman, O" sort="Sulaiman, O" uniqKey="Sulaiman O" first="O" last="Sulaiman">O. Sulaiman</name>
</noRegion>
<name sortKey="Fatmah, M S" sort="Fatmah, M S" uniqKey="Fatmah M" first="M. S" last="Fatmah">M. S Fatmah</name>
<name sortKey="Ismail, G" sort="Ismail, G" uniqKey="Ismail G" first="G" last="Ismail">G. Ismail</name>
<name sortKey="Khairul, A A" sort="Khairul, A A" uniqKey="Khairul A" first="A. A." last="Khairul">A. A. Khairul</name>
<name sortKey="Rahmah, N" sort="Rahmah, N" uniqKey="Rahmah N" first="N" last="Rahmah">N. Rahmah</name>
<name sortKey="Yusof, S" sort="Yusof, S" uniqKey="Yusof S" first="S" last="Yusof">S. Yusof</name>
</country>
</tree>
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</record>
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