IgG subclass responses to proinflammatory fraction of Brugia malayi in human filariasis
Identifieur interne : 004F25 ( Ncbi/Merge ); précédent : 004F24; suivant : 004F26IgG subclass responses to proinflammatory fraction of Brugia malayi in human filariasis
Auteurs : S. K. Joseph ; S. K. Verma ; M. K. Sahoo ; A. Sharma ; M. Srivastava ; M. V. R. Reddy ; P. K. MurthySource :
- The Indian Journal of Medical Research [ 0971-5916 ] ; 2012.
Abstract
Earlier we demonstrated that immunization with F6, a proinflammatory molecular fraction isolated from the human filarial parasite
Serum samples of normal individuals from filariasis non-endemic regions of India like Jammu & Kashmir, Uttarakhand, and Chandigarh [(NEN-W; n=10), healthy subjects from USA (NEN-U; n=10) and three categories of bancroftian filariasis cases from endemic areas: endemic normals (EN; n=10) with no symptoms and no microfilariae, asymptomatic microfilaremics (ASM; n=10) and chronic symptomatic amicrofilaremics (CL; n=10) were assayed for F6-specific IgG1, IgG2, IgG3 and IgG4 by ELISA using SDS-PAGE-isolated F6 fraction of
Significantly high levels of F6-specific IgG1, IgG2 and IgG3 were found in CL (
The high levels of F6 reactive IgG1, IgG2 and IgG3 in endemic normals and chronic symptomatic bancroftian patients, and IgG1 and IgG2 in asymptomatic microfilaraemics, suggest that F6 molecules of parasite are accessible in these subjects for IgG subclass-specific immune response and IgG2 may be related to pathogenesis. Studies using individual F6 molecules will be done to identify the molecule(s) involved in infection and protective immunity.
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PubMed: 22771594
PubMed Central: 3401695
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">IgG subclass responses to proinflammatory fraction of <italic>Brugia malayi</italic> in human filariasis</title><author><name sortKey="Joseph, S K" sort="Joseph, S K" uniqKey="Joseph S" first="S. K." last="Joseph">S. K. Joseph</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Verma, S K" sort="Verma, S K" uniqKey="Verma S" first="S. K." last="Verma">S. K. Verma</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Sahoo, M K" sort="Sahoo, M K" uniqKey="Sahoo M" first="M. K." last="Sahoo">M. K. Sahoo</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Sharma, A" sort="Sharma, A" uniqKey="Sharma A" first="A." last="Sharma">A. Sharma</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Srivastava, M" sort="Srivastava, M" uniqKey="Srivastava M" first="M." last="Srivastava">M. Srivastava</name><affiliation><nlm:aff id="aff2"><italic>Division of Biometry & Statistics, CSIR-Central Drug Research Institute, Lucknow, India</italic></nlm:aff></affiliation></author><author><name sortKey="Reddy, M V R" sort="Reddy, M V R" uniqKey="Reddy M" first="M. V. R." last="Reddy">M. V. R. Reddy</name><affiliation><nlm:aff id="aff3"><italic>Department of Biochemistry & JB Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical Sciences, Sevagram, India</italic></nlm:aff></affiliation></author><author><name sortKey="Murthy, P K" sort="Murthy, P K" uniqKey="Murthy P" first="P. K." last="Murthy">P. K. Murthy</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">22771594</idno><idno type="pmc">3401695</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3401695</idno><idno type="RBID">PMC:3401695</idno><date when="2012">2012</date><idno type="wicri:Area/Pmc/Corpus">003F16</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">003F16</idno><idno type="wicri:Area/Pmc/Curation">003F15</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">003F15</idno><idno type="wicri:Area/Pmc/Checkpoint">002961</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">002961</idno><idno type="wicri:Area/Ncbi/Merge">004F25</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">IgG subclass responses to proinflammatory fraction of <italic>Brugia malayi</italic> in human filariasis</title><author><name sortKey="Joseph, S K" sort="Joseph, S K" uniqKey="Joseph S" first="S. K." last="Joseph">S. K. Joseph</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Verma, S K" sort="Verma, S K" uniqKey="Verma S" first="S. K." last="Verma">S. K. Verma</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Sahoo, M K" sort="Sahoo, M K" uniqKey="Sahoo M" first="M. K." last="Sahoo">M. K. Sahoo</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Sharma, A" sort="Sharma, A" uniqKey="Sharma A" first="A." last="Sharma">A. Sharma</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Srivastava, M" sort="Srivastava, M" uniqKey="Srivastava M" first="M." last="Srivastava">M. Srivastava</name><affiliation><nlm:aff id="aff2"><italic>Division of Biometry & Statistics, CSIR-Central Drug Research Institute, Lucknow, India</italic></nlm:aff></affiliation></author><author><name sortKey="Reddy, M V R" sort="Reddy, M V R" uniqKey="Reddy M" first="M. V. R." last="Reddy">M. V. R. Reddy</name><affiliation><nlm:aff id="aff3"><italic>Department of Biochemistry & JB Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical Sciences, Sevagram, India</italic></nlm:aff></affiliation></author><author><name sortKey="Murthy, P K" sort="Murthy, P K" uniqKey="Murthy P" first="P. K." last="Murthy">P. K. Murthy</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">The Indian Journal of Medical Research</title><idno type="ISSN">0971-5916</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="st1"><title>Background & objectives:</title><p>Earlier we demonstrated that immunization with F6, a proinflammatory molecular fraction isolated from the human filarial parasite <italic>Brugia malayi</italic>, protected the host and eliminated the infection in <italic>Mastomys coucha</italic> by a Th1/Th2 response including IgG2a antibody response. Whether F6 molecules become accessible to human host during natural course of infection and elicit similar response is not known. The present study was undertaken to determine the profile of IgG subclasses specifically reactive to F6 in different categories of bancroftian filariasis cases to infer any relationship between the levels of a particular F6-specific IgG subclass and the infection or disease status.</p></sec><sec id="st2"><title>Methods:</title><p>Serum samples of normal individuals from filariasis non-endemic regions of India like Jammu & Kashmir, Uttarakhand, and Chandigarh [(NEN-W; n=10), healthy subjects from USA (NEN-U; n=10) and three categories of bancroftian filariasis cases from endemic areas: endemic normals (EN; n=10) with no symptoms and no microfilariae, asymptomatic microfilaremics (ASM; n=10) and chronic symptomatic amicrofilaremics (CL; n=10) were assayed for F6-specific IgG1, IgG2, IgG3 and IgG4 by ELISA using SDS-PAGE-isolated F6 fraction of <italic>B. malayi</italic> adult worms.</p></sec><sec id="st3"><title>Results:</title><p>Significantly high levels of F6-specific IgG1, IgG2 and IgG3 were found in CL (<italic>P</italic><0.001) and EN (<italic>P</italic><0.01-0.001) bancroftian filariasis cases compared to NEN-U. Significant levels of F6-specific IgG1 (<italic>P</italic><0.01) and IgG2 (<italic>P</italic><0.01) but not IgG3 were found in ASM cases compared to NEN-U. The most abundant was IgG2 which when compared to NEN-U, was significantly high in CL (<italic>P</italic><0.001) and EN cases (<italic>P</italic><0.001), followed by ASM (<italic>P</italic><0.01). F6-specific IgG4 response in EN, ASM and CL subjects was not significantly different from the levels of NEN-U. Among the non-endemic normals, the NEN-W subjects showed significant reactivity with IgG2 (<italic>P</italic><0.001) but not with IgG1, IgG3 and IgG4 as compared to NEN-U subjects. IgG subclass levels were different in different categories.</p></sec><sec id="st4"><title>Interpretation & conclusions:</title><p>The high levels of F6 reactive IgG1, IgG2 and IgG3 in endemic normals and chronic symptomatic bancroftian patients, and IgG1 and IgG2 in asymptomatic microfilaraemics, suggest that F6 molecules of parasite are accessible in these subjects for IgG subclass-specific immune response and IgG2 may be related to pathogenesis. Studies using individual F6 molecules will be done to identify the molecule(s) involved in infection and protective immunity.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Ravindran, B" uniqKey="Ravindran B">B Ravindran</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pincus, S" uniqKey="Pincus S">S Pincus</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dixit, S" uniqKey="Dixit S">S Dixit</name></author><author><name sortKey="Gaur, Rl" uniqKey="Gaur R">RL Gaur</name></author><author><name sortKey="Khan, Ma" uniqKey="Khan M">MA Khan</name></author><author><name sortKey="Saxena, Jk" uniqKey="Saxena J">JK Saxena</name></author><author><name sortKey="Murthy, Ps" uniqKey="Murthy P">PS Murthy</name></author><author><name sortKey="Murthy, Pk" uniqKey="Murthy P">PK 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Indian J Med Res</journal-id><journal-id journal-id-type="iso-abbrev">Indian J. Med. Res</journal-id><journal-id journal-id-type="publisher-id">IJMR</journal-id><journal-title-group><journal-title>The Indian Journal of Medical Research</journal-title></journal-title-group><issn pub-type="ppub">0971-5916</issn><publisher><publisher-name>Medknow Publications & Media Pvt Ltd</publisher-name><publisher-loc>India</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">22771594</article-id><article-id pub-id-type="pmc">3401695</article-id><article-id pub-id-type="publisher-id">IJMR-135-650</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject></subj-group></article-categories><title-group><article-title>IgG subclass responses to proinflammatory fraction of <italic>Brugia malayi</italic> in human filariasis</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Joseph</surname><given-names>S.K.</given-names></name><xref ref-type="aff" rid="aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Verma</surname><given-names>S.K.</given-names></name><xref ref-type="aff" rid="aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Sahoo</surname><given-names>M.K.</given-names></name><xref ref-type="aff" rid="aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Sharma</surname><given-names>A.</given-names></name><xref ref-type="aff" rid="aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Srivastava</surname><given-names>M.</given-names></name><xref ref-type="aff" rid="aff2">*</xref></contrib><contrib contrib-type="author"><name><surname>Reddy</surname><given-names>M.V.R.</given-names></name><xref ref-type="aff" rid="aff3">**</xref></contrib><contrib contrib-type="author"><name><surname>Murthy</surname><given-names>P.K.</given-names></name><xref ref-type="aff" rid="aff1"></xref><xref ref-type="corresp" rid="cor1"></xref></contrib></contrib-group><aff id="aff1"><italic>Division of Parasitology, CSIR-Central Drug Research Institute, Lucknow, India</italic></aff><aff id="aff2"><label>*</label><italic>Division of Biometry & Statistics, CSIR-Central Drug Research Institute, Lucknow, India</italic></aff><aff id="aff3"><label>**</label><italic>Department of Biochemistry & JB Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical Sciences, Sevagram, India</italic></aff><author-notes><corresp id="cor1"><italic>Reprint requests</italic>: Dr. P.K. Murthy, Chief Scientist, Parasitology Division, CSIR-Central Drug Research Institute, Lucknow 226 001, India e-mail: <email xlink:href="drpkmurthy@yahoo.com">drpkmurthy@yahoo.com</email><email xlink:href="k_murthy@cdri.res.in">k_murthy@cdri.res.in</email><email xlink:href="psr_murthy@yahoo.com">psr_murthy@yahoo.com</email></corresp></author-notes><pub-date pub-type="ppub"><month>5</month><year>2012</year></pub-date><volume>135</volume><issue>5</issue><fpage>650</fpage><lpage>655</lpage><history><date date-type="accepted"><day>24</day><month>2</month><year>2010</year></date></history><permissions><copyright-statement>Copyright: © The Indian Journal of Medical Research</copyright-statement><copyright-year>2012</copyright-year><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc-sa/3.0"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><abstract><sec id="st1"><title>Background & objectives:</title><p>Earlier we demonstrated that immunization with F6, a proinflammatory molecular fraction isolated from the human filarial parasite <italic>Brugia malayi</italic>, protected the host and eliminated the infection in <italic>Mastomys coucha</italic> by a Th1/Th2 response including IgG2a antibody response. Whether F6 molecules become accessible to human host during natural course of infection and elicit similar response is not known. The present study was undertaken to determine the profile of IgG subclasses specifically reactive to F6 in different categories of bancroftian filariasis cases to infer any relationship between the levels of a particular F6-specific IgG subclass and the infection or disease status.</p></sec><sec id="st2"><title>Methods:</title><p>Serum samples of normal individuals from filariasis non-endemic regions of India like Jammu & Kashmir, Uttarakhand, and Chandigarh [(NEN-W; n=10), healthy subjects from USA (NEN-U; n=10) and three categories of bancroftian filariasis cases from endemic areas: endemic normals (EN; n=10) with no symptoms and no microfilariae, asymptomatic microfilaremics (ASM; n=10) and chronic symptomatic amicrofilaremics (CL; n=10) were assayed for F6-specific IgG1, IgG2, IgG3 and IgG4 by ELISA using SDS-PAGE-isolated F6 fraction of <italic>B. malayi</italic> adult worms.</p></sec><sec id="st3"><title>Results:</title><p>Significantly high levels of F6-specific IgG1, IgG2 and IgG3 were found in CL (<italic>P</italic><0.001) and EN (<italic>P</italic><0.01-0.001) bancroftian filariasis cases compared to NEN-U. Significant levels of F6-specific IgG1 (<italic>P</italic><0.01) and IgG2 (<italic>P</italic><0.01) but not IgG3 were found in ASM cases compared to NEN-U. The most abundant was IgG2 which when compared to NEN-U, was significantly high in CL (<italic>P</italic><0.001) and EN cases (<italic>P</italic><0.001), followed by ASM (<italic>P</italic><0.01). F6-specific IgG4 response in EN, ASM and CL subjects was not significantly different from the levels of NEN-U. Among the non-endemic normals, the NEN-W subjects showed significant reactivity with IgG2 (<italic>P</italic><0.001) but not with IgG1, IgG3 and IgG4 as compared to NEN-U subjects. IgG subclass levels were different in different categories.</p></sec><sec id="st4"><title>Interpretation & conclusions:</title><p>The high levels of F6 reactive IgG1, IgG2 and IgG3 in endemic normals and chronic symptomatic bancroftian patients, and IgG1 and IgG2 in asymptomatic microfilaraemics, suggest that F6 molecules of parasite are accessible in these subjects for IgG subclass-specific immune response and IgG2 may be related to pathogenesis. Studies using individual F6 molecules will be done to identify the molecule(s) involved in infection and protective immunity.</p></sec></abstract><kwd-group><kwd><italic>Brugia malayi</italic></kwd><kwd>IgG subclasses</kwd><kwd>proinflammatory antigen</kwd></kwd-group></article-meta></front></pmc><affiliations><list></list><tree><noCountry><name sortKey="Joseph, S K" sort="Joseph, S K" uniqKey="Joseph S" first="S. K." last="Joseph">S. K. Joseph</name><name sortKey="Murthy, P K" sort="Murthy, P K" uniqKey="Murthy P" first="P. K." last="Murthy">P. K. Murthy</name><name sortKey="Reddy, M V R" sort="Reddy, M V R" uniqKey="Reddy M" first="M. V. R." last="Reddy">M. V. R. Reddy</name><name sortKey="Sahoo, M K" sort="Sahoo, M K" uniqKey="Sahoo M" first="M. K." last="Sahoo">M. K. Sahoo</name><name sortKey="Sharma, A" sort="Sharma, A" uniqKey="Sharma A" first="A." last="Sharma">A. Sharma</name><name sortKey="Srivastava, M" sort="Srivastava, M" uniqKey="Srivastava M" first="M." last="Srivastava">M. Srivastava</name><name sortKey="Verma, S K" sort="Verma, S K" uniqKey="Verma S" first="S. K." last="Verma">S. K. Verma</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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