Identification and characterisation of a novel repetitive antigen from Onchocerca spp.
Identifieur interne : 00B451 ( Ncbi/Curation ); précédent : 00B450; suivant : 00B452Identification and characterisation of a novel repetitive antigen from Onchocerca spp.
Auteurs : J. Catmull [Australie] ; D. Zhang ; F. Ruggiero ; D B Copeman ; D J MillerSource :
- Molecular and biochemical parasitology [ 0166-6851 ] ; 1994.
Descripteurs français
- KwdFr :
- ADN complémentaire (génétique), Animaux, Anticorps antihelminthe (sang), Antigènes d'helminthe (génétique), Antigènes d'helminthe (isolement et purification), Antigènes d'helminthe (métabolisme), Brugia malayi (immunologie), Données de séquences moléculaires, Femelle, Filariose lymphatique (immunologie), Filarioses (immunologie), Humains, Immunohistochimie, Microfilaria (génétique), Microfilaria (immunologie), Mâle, Onchocerca (génétique), Onchocerca (immunologie), Onchocerca volvulus (génétique), Onchocerca volvulus (immunologie), Onchocercose (immunologie), Onchocercose (parasitologie), Protéines d'helminthes (génétique), Protéines d'helminthes (immunologie), Protéines recombinantes (génétique), Protéines recombinantes (immunologie), Réactions croisées, Similitude de séquences d'acides aminés, Séquence d'acides aminés, Séquences répétées d'acides nucléiques, Wuchereria bancrofti (immunologie).
- MESH :
- génétique : ADN complémentaire, Antigènes d'helminthe, Microfilaria, Onchocerca, Onchocerca volvulus, Protéines d'helminthes, Protéines recombinantes.
- immunologie : Brugia malayi, Filariose lymphatique, Filarioses, Microfilaria, Onchocerca, Onchocerca volvulus, Onchocercose, Protéines d'helminthes, Protéines recombinantes, Wuchereria bancrofti.
- isolement et purification : Antigènes d'helminthe.
- métabolisme : Antigènes d'helminthe.
- parasitologie : Onchocercose.
- sang : Anticorps antihelminthe.
- Animaux, Données de séquences moléculaires, Femelle, Humains, Immunohistochimie, Mâle, Réactions croisées, Similitude de séquences d'acides aminés, Séquence d'acides aminés, Séquences répétées d'acides nucléiques.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Helminth (blood), Antigens, Helminth (genetics), Antigens, Helminth (isolation & purification), Antigens, Helminth (metabolism), Brugia malayi (immunology), Cross Reactions, DNA, Complementary (genetics), Elephantiasis, Filarial (immunology), Female, Filariasis (immunology), Helminth Proteins (genetics), Helminth Proteins (immunology), Humans, Immunohistochemistry, Male, Microfilariae (genetics), Microfilariae (immunology), Molecular Sequence Data, Onchocerca (genetics), Onchocerca (immunology), Onchocerca volvulus (genetics), Onchocerca volvulus (immunology), Onchocerciasis (immunology), Onchocerciasis (parasitology), Recombinant Proteins (genetics), Recombinant Proteins (immunology), Repetitive Sequences, Nucleic Acid, Sequence Homology, Amino Acid, Wuchereria bancrofti (immunology).
- MESH :
- chemical , blood : Antibodies, Helminth.
- chemical , genetics : Antigens, Helminth, DNA, Complementary, Helminth Proteins, Recombinant Proteins.
- chemical , immunology : Helminth Proteins, Recombinant Proteins.
- chemical , isolation & purification : Antigens, Helminth.
- chemical , metabolism : Antigens, Helminth.
- genetics : Microfilariae, Onchocerca, Onchocerca volvulus.
- immunology : Brugia malayi, Elephantiasis, Filarial, Filariasis, Microfilariae, Onchocerca, Onchocerca volvulus, Onchocerciasis, Wuchereria bancrofti.
- parasitology : Onchocerciasis.
- Amino Acid Sequence, Animals, Cross Reactions, Female, Humans, Immunohistochemistry, Male, Molecular Sequence Data, Repetitive Sequences, Nucleic Acid, Sequence Homology, Amino Acid.
Abstract
A novel repetitive antigen from the cattle parasite Onchocerca gibsoni was shown to be recognised by sera from humans infected with Onchocerca volvulus, Wuchereria bancroftii or Brugia malayi. The O. gibsoni protein was produced in a recombinant form, and antibodies raised to this protein used to screen cDNA libraries for O. volvulus. A series of clones were isolated which encoded repetitive regions very similar to those in O. gibsoni, but interspersed between these were longer repeating units which we have not so far found in O. gibsoni. The repetitive antigen was shown to be of high molecular weight and present only in the insoluble (membrane) fraction of O. gibsoni microfilariae. Immunofluorescence techniques demonstrated that the antigen was associated both with muscle and with specific membrane layers, including a peripheral layer which corresponds to either the outer hypodermis or an inner region of the cuticle in adult female O. gibsoni. In many respects, the proteins encoded by the O. gibsoni and O. volvulus cDNA clones resembled repetitive antigens from several distantly related eukaryotic parasites, and a possible common role in immune evasion is discussed.
PubMed: 8183322
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Ruggiero</name></author><author><name sortKey="Copeman, D B" sort="Copeman, D B" uniqKey="Copeman D" first="D B" last="Copeman">D B Copeman</name></author><author><name sortKey="Miller, D J" sort="Miller, D J" uniqKey="Miller D" first="D J" last="Miller">D J Miller</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1994">1994</date><idno type="RBID">pubmed:8183322</idno><idno type="pmid">8183322</idno><idno type="wicri:Area/PubMed/Corpus">005604</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">005604</idno><idno type="wicri:Area/PubMed/Curation">005604</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">005604</idno><idno type="wicri:Area/PubMed/Checkpoint">005604</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">005604</idno><idno type="wicri:Area/Ncbi/Merge">00B451</idno><idno type="wicri:Area/Ncbi/Curation">00B451</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Identification and characterisation of a novel repetitive antigen from Onchocerca spp.</title><author><name sortKey="Catmull, J" sort="Catmull, J" uniqKey="Catmull J" first="J" last="Catmull">J. Catmull</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry and Biochemistry, James Cook University of North Queensland, Townsville, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Chemistry and Biochemistry, James Cook University of North Queensland, Townsville</wicri:regionArea><wicri:noRegion>Townsville</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, D" sort="Zhang, D" uniqKey="Zhang D" first="D" last="Zhang">D. Zhang</name></author><author><name sortKey="Ruggiero, F" sort="Ruggiero, F" uniqKey="Ruggiero F" first="F" last="Ruggiero">F. Ruggiero</name></author><author><name sortKey="Copeman, D B" sort="Copeman, D B" uniqKey="Copeman D" first="D B" last="Copeman">D B Copeman</name></author><author><name sortKey="Miller, D J" sort="Miller, D J" uniqKey="Miller D" first="D J" last="Miller">D J Miller</name></author></analytic><series><title level="j">Molecular and biochemical parasitology</title><idno type="ISSN">0166-6851</idno><imprint><date when="1994" type="published">1994</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antibodies, Helminth (blood)</term><term>Antigens, Helminth (genetics)</term><term>Antigens, Helminth (isolation & purification)</term><term>Antigens, Helminth (metabolism)</term><term>Brugia malayi (immunology)</term><term>Cross Reactions</term><term>DNA, Complementary (genetics)</term><term>Elephantiasis, Filarial 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(génétique)</term><term>Antigènes d'helminthe (isolement et purification)</term><term>Antigènes d'helminthe (métabolisme)</term><term>Brugia malayi (immunologie)</term><term>Données de séquences moléculaires</term><term>Femelle</term><term>Filariose lymphatique (immunologie)</term><term>Filarioses (immunologie)</term><term>Humains</term><term>Immunohistochimie</term><term>Microfilaria (génétique)</term><term>Microfilaria (immunologie)</term><term>Mâle</term><term>Onchocerca (génétique)</term><term>Onchocerca (immunologie)</term><term>Onchocerca volvulus (génétique)</term><term>Onchocerca volvulus (immunologie)</term><term>Onchocercose (immunologie)</term><term>Onchocercose (parasitologie)</term><term>Protéines d'helminthes (génétique)</term><term>Protéines d'helminthes (immunologie)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (immunologie)</term><term>Réactions croisées</term><term>Similitude de séquences d'acides aminés</term><term>Séquence 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qualifier="isolement et purification" xml:lang="fr"><term>Antigènes d'helminthe</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antigènes d'helminthe</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Onchocercose</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Onchocerciasis</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antihelminthe</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Cross Reactions</term><term>Female</term><term>Humans</term><term>Immunohistochemistry</term><term>Male</term><term>Molecular Sequence Data</term><term>Repetitive Sequences, Nucleic Acid</term><term>Sequence Homology, Amino Acid</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Données de séquences moléculaires</term><term>Femelle</term><term>Humains</term><term>Immunohistochimie</term><term>Mâle</term><term>Réactions croisées</term><term>Similitude de séquences d'acides aminés</term><term>Séquence d'acides aminés</term><term>Séquences répétées d'acides nucléiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A novel repetitive antigen from the cattle parasite Onchocerca gibsoni was shown to be recognised by sera from humans infected with Onchocerca volvulus, Wuchereria bancroftii or Brugia malayi. The O. gibsoni protein was produced in a recombinant form, and antibodies raised to this protein used to screen cDNA libraries for O. volvulus. A series of clones were isolated which encoded repetitive regions very similar to those in O. gibsoni, but interspersed between these were longer repeating units which we have not so far found in O. gibsoni. The repetitive antigen was shown to be of high molecular weight and present only in the insoluble (membrane) fraction of O. gibsoni microfilariae. Immunofluorescence techniques demonstrated that the antigen was associated both with muscle and with specific membrane layers, including a peripheral layer which corresponds to either the outer hypodermis or an inner region of the cuticle in adult female O. gibsoni. In many respects, the proteins encoded by the O. gibsoni and O. volvulus cDNA clones resembled repetitive antigens from several distantly related eukaryotic parasites, and a possible common role in immune evasion is discussed.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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