Identification and characterisation of a novel repetitive antigen from Onchocerca spp.
Identifieur interne : 005604 ( PubMed/Corpus ); précédent : 005603; suivant : 005605Identification and characterisation of a novel repetitive antigen from Onchocerca spp.
Auteurs : J. Catmull ; D. Zhang ; F. Ruggiero ; D B Copeman ; D J MillerSource :
- Molecular and biochemical parasitology [ 0166-6851 ] ; 1994.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Helminth (blood), Antigens, Helminth (genetics), Antigens, Helminth (isolation & purification), Antigens, Helminth (metabolism), Brugia malayi (immunology), Cross Reactions, DNA, Complementary (genetics), Elephantiasis, Filarial (immunology), Female, Filariasis (immunology), Helminth Proteins (genetics), Helminth Proteins (immunology), Humans, Immunohistochemistry, Male, Microfilariae (genetics), Microfilariae (immunology), Molecular Sequence Data, Onchocerca (genetics), Onchocerca (immunology), Onchocerca volvulus (genetics), Onchocerca volvulus (immunology), Onchocerciasis (immunology), Onchocerciasis (parasitology), Recombinant Proteins (genetics), Recombinant Proteins (immunology), Repetitive Sequences, Nucleic Acid, Sequence Homology, Amino Acid, Wuchereria bancrofti (immunology).
- MESH :
- chemical , blood : Antibodies, Helminth.
- chemical , genetics : Antigens, Helminth, DNA, Complementary, Helminth Proteins, Recombinant Proteins.
- chemical , immunology : Helminth Proteins, Recombinant Proteins.
- chemical , isolation & purification : Antigens, Helminth.
- chemical , metabolism : Antigens, Helminth.
- genetics : Microfilariae, Onchocerca, Onchocerca volvulus.
- immunology : Brugia malayi, Elephantiasis, Filarial, Filariasis, Microfilariae, Onchocerca, Onchocerca volvulus, Onchocerciasis, Wuchereria bancrofti.
- parasitology : Onchocerciasis.
- Amino Acid Sequence, Animals, Cross Reactions, Female, Humans, Immunohistochemistry, Male, Molecular Sequence Data, Repetitive Sequences, Nucleic Acid, Sequence Homology, Amino Acid.
Abstract
A novel repetitive antigen from the cattle parasite Onchocerca gibsoni was shown to be recognised by sera from humans infected with Onchocerca volvulus, Wuchereria bancroftii or Brugia malayi. The O. gibsoni protein was produced in a recombinant form, and antibodies raised to this protein used to screen cDNA libraries for O. volvulus. A series of clones were isolated which encoded repetitive regions very similar to those in O. gibsoni, but interspersed between these were longer repeating units which we have not so far found in O. gibsoni. The repetitive antigen was shown to be of high molecular weight and present only in the insoluble (membrane) fraction of O. gibsoni microfilariae. Immunofluorescence techniques demonstrated that the antigen was associated both with muscle and with specific membrane layers, including a peripheral layer which corresponds to either the outer hypodermis or an inner region of the cuticle in adult female O. gibsoni. In many respects, the proteins encoded by the O. gibsoni and O. volvulus cDNA clones resembled repetitive antigens from several distantly related eukaryotic parasites, and a possible common role in immune evasion is discussed.
PubMed: 8183322
Links to Exploration step
pubmed:8183322Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification and characterisation of a novel repetitive antigen from Onchocerca spp.</title>
<author><name sortKey="Catmull, J" sort="Catmull, J" uniqKey="Catmull J" first="J" last="Catmull">J. Catmull</name>
<affiliation><nlm:affiliation>Department of Chemistry and Biochemistry, James Cook University of North Queensland, Townsville, Australia.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Zhang, D" sort="Zhang, D" uniqKey="Zhang D" first="D" last="Zhang">D. Zhang</name>
</author>
<author><name sortKey="Ruggiero, F" sort="Ruggiero, F" uniqKey="Ruggiero F" first="F" last="Ruggiero">F. Ruggiero</name>
</author>
<author><name sortKey="Copeman, D B" sort="Copeman, D B" uniqKey="Copeman D" first="D B" last="Copeman">D B Copeman</name>
</author>
<author><name sortKey="Miller, D J" sort="Miller, D J" uniqKey="Miller D" first="D J" last="Miller">D J Miller</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="1994">1994</date>
<idno type="RBID">pubmed:8183322</idno>
<idno type="pmid">8183322</idno>
<idno type="wicri:Area/PubMed/Corpus">005604</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">005604</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Identification and characterisation of a novel repetitive antigen from Onchocerca spp.</title>
<author><name sortKey="Catmull, J" sort="Catmull, J" uniqKey="Catmull J" first="J" last="Catmull">J. Catmull</name>
<affiliation><nlm:affiliation>Department of Chemistry and Biochemistry, James Cook University of North Queensland, Townsville, Australia.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Zhang, D" sort="Zhang, D" uniqKey="Zhang D" first="D" last="Zhang">D. Zhang</name>
</author>
<author><name sortKey="Ruggiero, F" sort="Ruggiero, F" uniqKey="Ruggiero F" first="F" last="Ruggiero">F. Ruggiero</name>
</author>
<author><name sortKey="Copeman, D B" sort="Copeman, D B" uniqKey="Copeman D" first="D B" last="Copeman">D B Copeman</name>
</author>
<author><name sortKey="Miller, D J" sort="Miller, D J" uniqKey="Miller D" first="D J" last="Miller">D J Miller</name>
</author>
</analytic>
<series><title level="j">Molecular and biochemical parasitology</title>
<idno type="ISSN">0166-6851</idno>
<imprint><date when="1994" type="published">1994</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Antibodies, Helminth (blood)</term>
<term>Antigens, Helminth (genetics)</term>
<term>Antigens, Helminth (isolation & purification)</term>
<term>Antigens, Helminth (metabolism)</term>
<term>Brugia malayi (immunology)</term>
<term>Cross Reactions</term>
<term>DNA, Complementary (genetics)</term>
<term>Elephantiasis, Filarial (immunology)</term>
<term>Female</term>
<term>Filariasis (immunology)</term>
<term>Helminth Proteins (genetics)</term>
<term>Helminth Proteins (immunology)</term>
<term>Humans</term>
<term>Immunohistochemistry</term>
<term>Male</term>
<term>Microfilariae (genetics)</term>
<term>Microfilariae (immunology)</term>
<term>Molecular Sequence Data</term>
<term>Onchocerca (genetics)</term>
<term>Onchocerca (immunology)</term>
<term>Onchocerca volvulus (genetics)</term>
<term>Onchocerca volvulus (immunology)</term>
<term>Onchocerciasis (immunology)</term>
<term>Onchocerciasis (parasitology)</term>
<term>Recombinant Proteins (genetics)</term>
<term>Recombinant Proteins (immunology)</term>
<term>Repetitive Sequences, Nucleic Acid</term>
<term>Sequence Homology, Amino Acid</term>
<term>Wuchereria bancrofti (immunology)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Helminth</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Helminth</term>
<term>DNA, Complementary</term>
<term>Helminth Proteins</term>
<term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Helminth Proteins</term>
<term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antigens, Helminth</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antigens, Helminth</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Microfilariae</term>
<term>Onchocerca</term>
<term>Onchocerca volvulus</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Brugia malayi</term>
<term>Elephantiasis, Filarial</term>
<term>Filariasis</term>
<term>Microfilariae</term>
<term>Onchocerca</term>
<term>Onchocerca volvulus</term>
<term>Onchocerciasis</term>
<term>Wuchereria bancrofti</term>
</keywords>
<keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Onchocerciasis</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Cross Reactions</term>
<term>Female</term>
<term>Humans</term>
<term>Immunohistochemistry</term>
<term>Male</term>
<term>Molecular Sequence Data</term>
<term>Repetitive Sequences, Nucleic Acid</term>
<term>Sequence Homology, Amino Acid</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">A novel repetitive antigen from the cattle parasite Onchocerca gibsoni was shown to be recognised by sera from humans infected with Onchocerca volvulus, Wuchereria bancroftii or Brugia malayi. The O. gibsoni protein was produced in a recombinant form, and antibodies raised to this protein used to screen cDNA libraries for O. volvulus. A series of clones were isolated which encoded repetitive regions very similar to those in O. gibsoni, but interspersed between these were longer repeating units which we have not so far found in O. gibsoni. The repetitive antigen was shown to be of high molecular weight and present only in the insoluble (membrane) fraction of O. gibsoni microfilariae. Immunofluorescence techniques demonstrated that the antigen was associated both with muscle and with specific membrane layers, including a peripheral layer which corresponds to either the outer hypodermis or an inner region of the cuticle in adult female O. gibsoni. In many respects, the proteins encoded by the O. gibsoni and O. volvulus cDNA clones resembled repetitive antigens from several distantly related eukaryotic parasites, and a possible common role in immune evasion is discussed.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8183322</PMID>
<DateCreated><Year>1994</Year>
<Month>06</Month>
<Day>16</Day>
</DateCreated>
<DateCompleted><Year>1994</Year>
<Month>06</Month>
<Day>16</Day>
</DateCompleted>
<DateRevised><Year>2016</Year>
<Month>11</Month>
<Day>23</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0166-6851</ISSN>
<JournalIssue CitedMedium="Print"><Volume>63</Volume>
<Issue>1</Issue>
<PubDate><Year>1994</Year>
<Month>Jan</Month>
</PubDate>
</JournalIssue>
<Title>Molecular and biochemical parasitology</Title>
<ISOAbbreviation>Mol. Biochem. Parasitol.</ISOAbbreviation>
</Journal>
<ArticleTitle>Identification and characterisation of a novel repetitive antigen from Onchocerca spp.</ArticleTitle>
<Pagination><MedlinePgn>49-57</MedlinePgn>
</Pagination>
<Abstract><AbstractText>A novel repetitive antigen from the cattle parasite Onchocerca gibsoni was shown to be recognised by sera from humans infected with Onchocerca volvulus, Wuchereria bancroftii or Brugia malayi. The O. gibsoni protein was produced in a recombinant form, and antibodies raised to this protein used to screen cDNA libraries for O. volvulus. A series of clones were isolated which encoded repetitive regions very similar to those in O. gibsoni, but interspersed between these were longer repeating units which we have not so far found in O. gibsoni. The repetitive antigen was shown to be of high molecular weight and present only in the insoluble (membrane) fraction of O. gibsoni microfilariae. Immunofluorescence techniques demonstrated that the antigen was associated both with muscle and with specific membrane layers, including a peripheral layer which corresponds to either the outer hypodermis or an inner region of the cuticle in adult female O. gibsoni. In many respects, the proteins encoded by the O. gibsoni and O. volvulus cDNA clones resembled repetitive antigens from several distantly related eukaryotic parasites, and a possible common role in immune evasion is discussed.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Catmull</LastName>
<ForeName>J</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Department of Chemistry and Biochemistry, James Cook University of North Queensland, Townsville, Australia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>D</ForeName>
<Initials>D</Initials>
</Author>
<Author ValidYN="Y"><LastName>Ruggiero</LastName>
<ForeName>F</ForeName>
<Initials>F</Initials>
</Author>
<Author ValidYN="Y"><LastName>Copeman</LastName>
<ForeName>D B</ForeName>
<Initials>DB</Initials>
</Author>
<Author ValidYN="Y"><LastName>Miller</LastName>
<ForeName>D J</ForeName>
<Initials>DJ</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName>
<AccessionNumberList><AccessionNumber>U01040</AccessionNumber>
<AccessionNumber>U01302</AccessionNumber>
<AccessionNumber>U01315</AccessionNumber>
<AccessionNumber>U01316</AccessionNumber>
<AccessionNumber>U01324</AccessionNumber>
</AccessionNumberList>
</DataBank>
</DataBankList>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>Netherlands</Country>
<MedlineTA>Mol Biochem Parasitol</MedlineTA>
<NlmUniqueID>8006324</NlmUniqueID>
<ISSNLinking>0166-6851</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000909">Antibodies, Helminth</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000947">Antigens, Helminth</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D015801">Helminth Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000909" MajorTopicYN="N">Antibodies, Helminth</DescriptorName>
<QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000947" MajorTopicYN="N">Antigens, Helminth</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017178" MajorTopicYN="N">Brugia malayi</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003429" MajorTopicYN="N">Cross Reactions</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004605" MajorTopicYN="N">Elephantiasis, Filarial</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005368" MajorTopicYN="N">Filariasis</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015801" MajorTopicYN="N">Helminth Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007150" MajorTopicYN="N">Immunohistochemistry</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008842" MajorTopicYN="N">Microfilariae</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009854" MajorTopicYN="N">Onchocerca</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017181" MajorTopicYN="N">Onchocerca volvulus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009855" MajorTopicYN="N">Onchocerciasis</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012091" MajorTopicYN="N">Repetitive Sequences, Nucleic Acid</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017386" MajorTopicYN="N">Sequence Homology, Amino Acid</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014958" MajorTopicYN="N">Wuchereria bancrofti</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1994</Year>
<Month>1</Month>
<Day>1</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>1994</Year>
<Month>1</Month>
<Day>1</Day>
<Hour>0</Hour>
<Minute>1</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>1994</Year>
<Month>1</Month>
<Day>1</Day>
<Hour>0</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">8183322</ArticleId>
<ArticleId IdType="pii">0166-6851(94)90007-8</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 005604 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 005604 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= LymphedemaV1 |flux= PubMed |étape= Corpus |type= RBID |clé= pubmed:8183322 |texte= Identification and characterisation of a novel repetitive antigen from Onchocerca spp. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:8183322" \ | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \ | NlmPubMed2Wicri -a LymphedemaV1
![]() | This area was generated with Dilib version V0.6.31. | ![]() |