Vascular endothelial growth factor receptor-1 mediates migration of human colorectal carcinoma cells by activation of Src family kinases
Identifieur interne : 002226 ( Ncbi/Curation ); précédent : 002225; suivant : 002227Vascular endothelial growth factor receptor-1 mediates migration of human colorectal carcinoma cells by activation of Src family kinases
Auteurs : D P Lesslie [États-Unis] ; J M Summy [États-Unis] ; N U Parikh [États-Unis] ; F. Fan [États-Unis] ; J G Trevino [États-Unis] ; T K Sawyer [États-Unis] ; C A Metcalf [États-Unis] ; W C Shakespeare [États-Unis] ; D J Hicklin [États-Unis] ; L M Ellis [États-Unis] ; G E Gallick [États-Unis]Source :
- British Journal of Cancer [ 0007-0920 ] ; 2006.
Abstract
Vascular endothelial growth factor (VEGF) is the predominant pro-angiogenic cytokine in human malignancy, and its expression correlates with disease recurrence and poor outcomes in patients with colorectal cancer. Recently, expression of vascular endothelial growth factor receptors (VEGFRs) has been observed on tumours of epithelial origin, including those arising in the colon, but the molecular mechanisms governing potential VEGF-driven biologic functioning in these tumours are not well characterised. In this report, we investigated the role of Src family kinases (SFKs) in VEGF-mediated signalling in human colorectal carcinoma (CRC) cell lines. Vascular endothelial growth factor specifically activated SFKs in HT29 and KM12L4 CRC cell lines. Further, VEGF stimulation resulted in enhanced cellular migration, which was effectively blocked by pharmacologic inhibition of VEGFR-1 or Src kinase. Correspondingly, migration studies using siRNA clones with reduced Src expression confirmed the requirement for Src in VEGF-induced migration in these cells. Furthermore, VEGF treatment enhanced VEGFR-1/SFK complex formation and increased tyrosine phosphorylation of focal adhesion kinase, p130 cas and paxillin. Finally, we demonstrate that VEGF-induced migration is not due, at least in part, to VEGF acting as a mitogen. These results suggest that VEGFR-1 promotes migration of tumour cells through a Src-dependent pathway linked to activation of focal adhesion components that regulate this process.
Url:
DOI: 10.1038/sj.bjc.6603143
PubMed: 16685275
PubMed Central: 2361330
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000110
- to stream Pmc, to step Curation: Pour aller vers cette notice dans l'étape Curation :000110
- to stream Pmc, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :003C07
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :002226
Links to Exploration step
PMC:2361330Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Vascular endothelial growth factor receptor-1 mediates migration of human colorectal carcinoma cells by activation of Src family kinases</title><author><name sortKey="Lesslie, D P" sort="Lesslie, D P" uniqKey="Lesslie D" first="D P" last="Lesslie">D P Lesslie</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Surgical Oncology, The University of Texas MD Anderson Cancer Center</institution> 1515 Holcombe Boulevard, Houston, TX,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Department of Cancer Biology</institution> 1515 Holcombe Boulevard, Houston, TX,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Summy, J M" sort="Summy, J M" uniqKey="Summy J" first="J M" last="Summy">J M Summy</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Department of Cancer Biology</institution> 1515 Holcombe Boulevard, Houston, TX,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Parikh, N U" sort="Parikh, N U" uniqKey="Parikh N" first="N U" last="Parikh">N U Parikh</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Department of Cancer Biology</institution> 1515 Holcombe Boulevard, Houston, TX,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Fan, F" sort="Fan, F" uniqKey="Fan F" first="F" last="Fan">F. Fan</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Department of Cancer Biology</institution> 1515 Holcombe Boulevard, Houston, TX,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Trevino, J G" sort="Trevino, J G" uniqKey="Trevino J" first="J G" last="Trevino">J G Trevino</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Surgical Oncology, The University of Texas MD Anderson Cancer Center</institution> 1515 Holcombe Boulevard, Houston, TX,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Department of Cancer Biology</institution> 1515 Holcombe Boulevard, Houston, TX,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Sawyer, T K" sort="Sawyer, T K" uniqKey="Sawyer T" first="T K" last="Sawyer">T K Sawyer</name><affiliation wicri:level="1"><nlm:aff id="aff3"><institution>Ariad Pharmaceuticals</institution> Cambridge, MA,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Metcalf, C A" sort="Metcalf, C A" uniqKey="Metcalf C" first="C A" last="Metcalf">C A Metcalf</name><affiliation wicri:level="1"><nlm:aff id="aff3"><institution>Ariad Pharmaceuticals</institution> Cambridge, MA,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Shakespeare, W C" sort="Shakespeare, W C" uniqKey="Shakespeare W" first="W C" last="Shakespeare">W C Shakespeare</name><affiliation wicri:level="1"><nlm:aff id="aff3"><institution>Ariad Pharmaceuticals</institution> Cambridge, MA,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Hicklin, D J" sort="Hicklin, D J" uniqKey="Hicklin D" first="D J" last="Hicklin">D J Hicklin</name><affiliation wicri:level="1"><nlm:aff id="aff4"><institution>ImClone Systems</institution> New York, NY,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Ellis, L M" sort="Ellis, L M" uniqKey="Ellis L" first="L M" last="Ellis">L M Ellis</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Surgical Oncology, The University of Texas MD Anderson Cancer Center</institution> 1515 Holcombe Boulevard, Houston, TX,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Department of Cancer Biology</institution> 1515 Holcombe Boulevard, Houston, TX,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Gallick, G E" sort="Gallick, G E" uniqKey="Gallick G" first="G E" last="Gallick">G E Gallick</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Department of Cancer Biology</institution> 1515 Holcombe Boulevard, Houston, TX,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">16685275</idno><idno type="pmc">2361330</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2361330</idno><idno type="RBID">PMC:2361330</idno><idno type="doi">10.1038/sj.bjc.6603143</idno><date when="2006">2006</date><idno type="wicri:Area/Pmc/Corpus">000110</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000110</idno><idno type="wicri:Area/Pmc/Curation">000110</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000110</idno><idno type="wicri:Area/Pmc/Checkpoint">003C07</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">003C07</idno><idno type="wicri:Area/Ncbi/Merge">002226</idno><idno type="wicri:Area/Ncbi/Curation">002226</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Vascular endothelial growth factor receptor-1 mediates migration of human colorectal carcinoma cells by activation of Src family kinases</title><author><name sortKey="Lesslie, D P" sort="Lesslie, D P" uniqKey="Lesslie D" first="D P" last="Lesslie">D P Lesslie</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Surgical Oncology, The University of Texas MD Anderson Cancer Center</institution> 1515 Holcombe Boulevard, Houston, TX,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Department of Cancer Biology</institution> 1515 Holcombe Boulevard, Houston, TX,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Summy, J M" sort="Summy, J M" uniqKey="Summy J" first="J M" last="Summy">J M Summy</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Department of Cancer Biology</institution> 1515 Holcombe Boulevard, Houston, TX,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Parikh, N U" sort="Parikh, N U" uniqKey="Parikh N" first="N U" last="Parikh">N U Parikh</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Department of Cancer Biology</institution> 1515 Holcombe Boulevard, Houston, TX,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Fan, F" sort="Fan, F" uniqKey="Fan F" first="F" last="Fan">F. Fan</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Department of Cancer Biology</institution> 1515 Holcombe Boulevard, Houston, TX,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Trevino, J G" sort="Trevino, J G" uniqKey="Trevino J" first="J G" last="Trevino">J G Trevino</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Surgical Oncology, The University of Texas MD Anderson Cancer Center</institution> 1515 Holcombe Boulevard, Houston, TX,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Department of Cancer Biology</institution> 1515 Holcombe Boulevard, Houston, TX,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Sawyer, T K" sort="Sawyer, T K" uniqKey="Sawyer T" first="T K" last="Sawyer">T K Sawyer</name><affiliation wicri:level="1"><nlm:aff id="aff3"><institution>Ariad Pharmaceuticals</institution> Cambridge, MA,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Metcalf, C A" sort="Metcalf, C A" uniqKey="Metcalf C" first="C A" last="Metcalf">C A Metcalf</name><affiliation wicri:level="1"><nlm:aff id="aff3"><institution>Ariad Pharmaceuticals</institution> Cambridge, MA,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Shakespeare, W C" sort="Shakespeare, W C" uniqKey="Shakespeare W" first="W C" last="Shakespeare">W C Shakespeare</name><affiliation wicri:level="1"><nlm:aff id="aff3"><institution>Ariad Pharmaceuticals</institution> Cambridge, MA,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Hicklin, D J" sort="Hicklin, D J" uniqKey="Hicklin D" first="D J" last="Hicklin">D J Hicklin</name><affiliation wicri:level="1"><nlm:aff id="aff4"><institution>ImClone Systems</institution> New York, NY,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Ellis, L M" sort="Ellis, L M" uniqKey="Ellis L" first="L M" last="Ellis">L M Ellis</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Department of Surgical Oncology, The University of Texas MD Anderson Cancer Center</institution> 1515 Holcombe Boulevard, Houston, TX,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Department of Cancer Biology</institution> 1515 Holcombe Boulevard, Houston, TX,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Gallick, G E" sort="Gallick, G E" uniqKey="Gallick G" first="G E" last="Gallick">G E Gallick</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Department of Cancer Biology</institution> 1515 Holcombe Boulevard, Houston, TX,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">British Journal of Cancer</title><idno type="ISSN">0007-0920</idno><idno type="eISSN">1532-1827</idno><imprint><date when="2006">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Vascular endothelial growth factor (VEGF) is the predominant pro-angiogenic cytokine in human malignancy, and its expression correlates with disease recurrence and poor outcomes in patients with colorectal cancer. Recently, expression of vascular endothelial growth factor receptors (VEGFRs) has been observed on tumours of epithelial origin, including those arising in the colon, but the molecular mechanisms governing potential VEGF-driven biologic functioning in these tumours are not well characterised. In this report, we investigated the role of Src family kinases (SFKs) in VEGF-mediated signalling in human colorectal carcinoma (CRC) cell lines. Vascular endothelial growth factor specifically activated SFKs in HT29 and KM12L4 CRC cell lines. Further, VEGF stimulation resulted in enhanced cellular migration, which was effectively blocked by pharmacologic inhibition of VEGFR-1 or Src kinase. Correspondingly, migration studies using siRNA clones with reduced Src expression confirmed the requirement for Src in VEGF-induced migration in these cells. Furthermore, VEGF treatment enhanced VEGFR-1/SFK complex formation and increased tyrosine phosphorylation of focal adhesion kinase, p130 cas and paxillin. Finally, we demonstrate that VEGF-induced migration is not due, at least in part, to VEGF acting as a mitogen. These results suggest that VEGFR-1 promotes migration of tumour cells through a Src-dependent pathway linked to activation of focal adhesion components that regulate this process.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Ncbi/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002226 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 002226 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= Ncbi
|étape= Curation
|type= RBID
|clé= PMC:2361330
|texte= Vascular endothelial growth factor receptor-1 mediates migration of human colorectal carcinoma cells by activation of Src family kinases
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i -Sk "pubmed:16685275" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |