Role of Angiopoietin-2 in Corneal Lymphangiogenesis
Identifieur interne : 006626 ( Ncbi/Checkpoint ); précédent : 006625; suivant : 006627Role of Angiopoietin-2 in Corneal Lymphangiogenesis
Auteurs : Don Yuen [États-Unis] ; Sammy Grimaldo [États-Unis] ; Roberto Sessa [États-Unis] ; Tatiana Ecoiffier [États-Unis] ; Tan Truong [États-Unis] ; Eric Huang [États-Unis] ; Michael Bernas [États-Unis] ; Sarah Daley [États-Unis] ; Marlys Witte [États-Unis] ; Lu Chen [États-Unis]Source :
- Investigative Ophthalmology & Visual Science [ 0146-0404 ] ; 2014.
Abstract
Lymphatic research has progressed rapidly in recent years. Lymphatic dysfunction has been found in myriad disorders from cancer metastasis to transplant rejection; however, effective treatment for lymphatic disorders is still limited. This study investigates the role of angiopoietin-2 (Ang-2) in corneal inflammatory lymphangiogenesis (LG) in vivo and in lymphatic endothelial cell (LEC) functions in vitro.
Standard suture placement model was used to study Ang-2 expression in inflamed cornea, and corneal LG and hemangiogenesis (HG) responses in Ang-2 knockout mice. Moreover, human LEC culture system was used to examine the effect of Ang-2 gene knockdown on LEC functions using small interfering RNAs (siRNAs). The effect of siRNA treatment on corneal LG was also assessed in vivo.
Angiopoietin-2 was expressed on lymphatic vessels and macrophages in inflamed cornea. While corneal LG response was abolished in Ang-2 knockout mice, the HG response was also significantly suppressed with disorganized patterning. Moreover, anti-Ang-2 treatment inhibited LEC proliferation and capillary tube formation in vitro and corneal LG in vivo.
Angiopoietin-2 is critically involved in lymphatic processes in vivo and in vitro. Further investigation of the Ang-2 pathway may provide novel insights and therapeutic strategies for lymphatic-related disorders, which occur both inside and outside the eye.
Url:
DOI: 10.1167/iovs.13-13779
PubMed: 24781940
PubMed Central: 4039380
Affiliations:
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Arizona</wicri:regionArea><placeName><region type="state">Arizona</region></placeName></affiliation></author><author><name sortKey="Chen, Lu" sort="Chen, Lu" uniqKey="Chen L" first="Lu" last="Chen">Lu Chen</name><affiliation wicri:level="2"><nlm:aff id="aff1">Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, Berkeley, California, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, Berkeley, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff2">Graduate Group in Vision Science, University of California, Berkeley, Berkeley, California, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Graduate Group in Vision Science, 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wicri:level="2"><nlm:aff id="aff1">Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, Berkeley, California, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, Berkeley, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff2">Graduate Group in Vision Science, University of California, Berkeley, Berkeley, California, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Graduate Group in Vision Science, University of California, Berkeley, Berkeley, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Huang, Eric" sort="Huang, Eric" uniqKey="Huang E" first="Eric" last="Huang">Eric Huang</name><affiliation wicri:level="2"><nlm:aff id="aff1">Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, Berkeley, California, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, Berkeley, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Bernas, Michael" sort="Bernas, Michael" uniqKey="Bernas M" first="Michael" last="Bernas">Michael Bernas</name><affiliation wicri:level="2"><nlm:aff id="aff3">Department of Surgery, School of Medicine, University of Arizona, Tucson, Arizona, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Surgery, School of Medicine, University of Arizona, Tucson, Arizona</wicri:regionArea><placeName><region type="state">Arizona</region></placeName></affiliation></author><author><name sortKey="Daley, Sarah" sort="Daley, Sarah" uniqKey="Daley S" first="Sarah" last="Daley">Sarah Daley</name><affiliation wicri:level="2"><nlm:aff id="aff3">Department of Surgery, School of Medicine, University of Arizona, Tucson, Arizona, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Surgery, School of Medicine, University of Arizona, Tucson, Arizona</wicri:regionArea><placeName><region type="state">Arizona</region></placeName></affiliation></author><author><name sortKey="Witte, Marlys" sort="Witte, Marlys" uniqKey="Witte M" first="Marlys" last="Witte">Marlys Witte</name><affiliation wicri:level="2"><nlm:aff id="aff3">Department of Surgery, School of Medicine, University of Arizona, Tucson, Arizona, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Surgery, School of Medicine, University of Arizona, Tucson, Arizona</wicri:regionArea><placeName><region type="state">Arizona</region></placeName></affiliation></author><author><name sortKey="Chen, Lu" sort="Chen, Lu" uniqKey="Chen L" first="Lu" last="Chen">Lu Chen</name><affiliation wicri:level="2"><nlm:aff id="aff1">Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, Berkeley, California, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, Berkeley, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff2">Graduate Group in Vision Science, University of California, Berkeley, Berkeley, California, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Graduate Group in Vision Science, University of California, Berkeley, Berkeley, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author></analytic><series><title level="j">Investigative Ophthalmology & Visual Science</title><idno type="ISSN">0146-0404</idno><idno type="eISSN">1552-5783</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="st1"><title>Purpose.</title><p>Lymphatic research has progressed rapidly in recent years. Lymphatic dysfunction has been found in myriad disorders from cancer metastasis to transplant rejection; however, effective treatment for lymphatic disorders is still limited. This study investigates the role of angiopoietin-2 (Ang-2) in corneal inflammatory lymphangiogenesis (LG) in vivo and in lymphatic endothelial cell (LEC) functions in vitro.</p></sec><sec id="st2"><title>Methods.</title><p>Standard suture placement model was used to study Ang-2 expression in inflamed cornea, and corneal LG and hemangiogenesis (HG) responses in Ang-2 knockout mice. Moreover, human LEC culture system was used to examine the effect of Ang-2 gene knockdown on LEC functions using small interfering RNAs (siRNAs). The effect of siRNA treatment on corneal LG was also assessed in vivo.</p></sec><sec id="st3"><title>Results.</title><p>Angiopoietin-2 was expressed on lymphatic vessels and macrophages in inflamed cornea. While corneal LG response was abolished in Ang-2 knockout mice, the HG response was also significantly suppressed with disorganized patterning. Moreover, anti-Ang-2 treatment inhibited LEC proliferation and capillary tube formation in vitro and corneal LG in vivo.</p></sec><sec id="st4"><title>Conclusions.</title><p>Angiopoietin-2 is critically involved in lymphatic processes in vivo and in vitro. Further investigation of the Ang-2 pathway may provide novel insights and therapeutic strategies for lymphatic-related disorders, which occur both inside and outside the eye.</p></sec></div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Arizona</li><li>Californie</li></region></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Yuen, Don" sort="Yuen, Don" uniqKey="Yuen D" first="Don" last="Yuen">Don Yuen</name></region><name sortKey="Bernas, Michael" sort="Bernas, Michael" uniqKey="Bernas M" first="Michael" last="Bernas">Michael Bernas</name><name sortKey="Chen, Lu" sort="Chen, Lu" uniqKey="Chen L" first="Lu" last="Chen">Lu Chen</name><name sortKey="Chen, Lu" sort="Chen, Lu" uniqKey="Chen L" first="Lu" last="Chen">Lu Chen</name><name sortKey="Daley, Sarah" sort="Daley, Sarah" uniqKey="Daley S" first="Sarah" last="Daley">Sarah Daley</name><name sortKey="Ecoiffier, Tatiana" sort="Ecoiffier, Tatiana" uniqKey="Ecoiffier T" first="Tatiana" last="Ecoiffier">Tatiana Ecoiffier</name><name sortKey="Grimaldo, Sammy" sort="Grimaldo, Sammy" uniqKey="Grimaldo S" first="Sammy" last="Grimaldo">Sammy Grimaldo</name><name sortKey="Huang, Eric" sort="Huang, Eric" uniqKey="Huang E" first="Eric" last="Huang">Eric Huang</name><name sortKey="Sessa, Roberto" sort="Sessa, Roberto" uniqKey="Sessa R" first="Roberto" last="Sessa">Roberto Sessa</name><name sortKey="Truong, Tan" sort="Truong, Tan" uniqKey="Truong T" first="Tan" last="Truong">Tan Truong</name><name sortKey="Truong, Tan" sort="Truong, Tan" uniqKey="Truong T" first="Tan" last="Truong">Tan Truong</name><name sortKey="Witte, Marlys" sort="Witte, Marlys" uniqKey="Witte M" first="Marlys" last="Witte">Marlys Witte</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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