Role of Angiopoietin-2 in Corneal Lymphangiogenesis
Identifieur interne : 006626 ( Ncbi/Curation ); précédent : 006625; suivant : 006627Role of Angiopoietin-2 in Corneal Lymphangiogenesis
Auteurs : Don Yuen [États-Unis] ; Sammy Grimaldo [États-Unis] ; Roberto Sessa [États-Unis] ; Tatiana Ecoiffier [États-Unis] ; Tan Truong [États-Unis] ; Eric Huang [États-Unis] ; Michael Bernas [États-Unis] ; Sarah Daley [États-Unis] ; Marlys Witte [États-Unis] ; Lu Chen [États-Unis]Source :
- Investigative Ophthalmology & Visual Science [ 0146-0404 ] ; 2014.
Abstract
Lymphatic research has progressed rapidly in recent years. Lymphatic dysfunction has been found in myriad disorders from cancer metastasis to transplant rejection; however, effective treatment for lymphatic disorders is still limited. This study investigates the role of angiopoietin-2 (Ang-2) in corneal inflammatory lymphangiogenesis (LG) in vivo and in lymphatic endothelial cell (LEC) functions in vitro.
Standard suture placement model was used to study Ang-2 expression in inflamed cornea, and corneal LG and hemangiogenesis (HG) responses in Ang-2 knockout mice. Moreover, human LEC culture system was used to examine the effect of Ang-2 gene knockdown on LEC functions using small interfering RNAs (siRNAs). The effect of siRNA treatment on corneal LG was also assessed in vivo.
Angiopoietin-2 was expressed on lymphatic vessels and macrophages in inflamed cornea. While corneal LG response was abolished in Ang-2 knockout mice, the HG response was also significantly suppressed with disorganized patterning. Moreover, anti-Ang-2 treatment inhibited LEC proliferation and capillary tube formation in vitro and corneal LG in vivo.
Angiopoietin-2 is critically involved in lymphatic processes in vivo and in vitro. Further investigation of the Ang-2 pathway may provide novel insights and therapeutic strategies for lymphatic-related disorders, which occur both inside and outside the eye.
Url:
DOI: 10.1167/iovs.13-13779
PubMed: 24781940
PubMed Central: 4039380
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<front><div type="abstract" xml:lang="en"><sec id="st1"><title>Purpose.</title>
<p>Lymphatic research has progressed rapidly in recent years. Lymphatic dysfunction has been found in myriad disorders from cancer metastasis to transplant rejection; however, effective treatment for lymphatic disorders is still limited. This study investigates the role of angiopoietin-2 (Ang-2) in corneal inflammatory lymphangiogenesis (LG) in vivo and in lymphatic endothelial cell (LEC) functions in vitro.</p>
</sec>
<sec id="st2"><title>Methods.</title>
<p>Standard suture placement model was used to study Ang-2 expression in inflamed cornea, and corneal LG and hemangiogenesis (HG) responses in Ang-2 knockout mice. Moreover, human LEC culture system was used to examine the effect of Ang-2 gene knockdown on LEC functions using small interfering RNAs (siRNAs). The effect of siRNA treatment on corneal LG was also assessed in vivo.</p>
</sec>
<sec id="st3"><title>Results.</title>
<p>Angiopoietin-2 was expressed on lymphatic vessels and macrophages in inflamed cornea. While corneal LG response was abolished in Ang-2 knockout mice, the HG response was also significantly suppressed with disorganized patterning. Moreover, anti-Ang-2 treatment inhibited LEC proliferation and capillary tube formation in vitro and corneal LG in vivo.</p>
</sec>
<sec id="st4"><title>Conclusions.</title>
<p>Angiopoietin-2 is critically involved in lymphatic processes in vivo and in vitro. Further investigation of the Ang-2 pathway may provide novel insights and therapeutic strategies for lymphatic-related disorders, which occur both inside and outside the eye.</p>
</sec>
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