Effects of heat stress on the expression of the coxsackievirus and adenovirus receptor in mouse skin keratinocytes
Identifieur interne : 005E49 ( Ncbi/Checkpoint ); précédent : 005E48; suivant : 005E50Effects of heat stress on the expression of the coxsackievirus and adenovirus receptor in mouse skin keratinocytes
Auteurs : Xiangdong Deng ; Chiyu Jia ; Fuxing Chen ; Junquan Liu ; Zonghai ZhouSource :
- Experimental and Therapeutic Medicine [ 1792-0981 ] ; 2013.
Abstract
The aim of this study was to investigate the effects of heat stress on the expression of the coxsackievirus and adenovirus receptor (CAR) in mouse skin keratinocytes. Twenty BALB/c mice were randomly divided into two groups: the sham heat (control) and scald groups. Skin specimens were obtained 6 h after the treatments. Changes in the expression of CAR in skin keratinocyte samples were detected by immunohistochemistry, quantitative polymerase chain reaction and western blotting. In an
Url:
DOI: 10.3892/etm.2013.1230
PubMed: 24137310
PubMed Central: 3797294
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Effects of heat stress on the expression of the coxsackievirus and adenovirus receptor in mouse skin keratinocytes</title><author><name sortKey="Deng, Xiangdong" sort="Deng, Xiangdong" uniqKey="Deng X" first="Xiangdong" last="Deng">Xiangdong Deng</name><affiliation><nlm:aff id="af1-etm-06-04-1029">The Graduate School, Chinese People’s Liberation Army Medical College, Beijing 100039;</nlm:aff><wicri:noCountry code="subfield">Beijing 100039</wicri:noCountry></affiliation></author><author><name sortKey="Jia, Chiyu" sort="Jia, Chiyu" uniqKey="Jia C" first="Chiyu" last="Jia">Chiyu Jia</name><affiliation><nlm:aff id="af2-etm-06-04-1029">Plastic Beauty and Burn Repair Center, The 309th Hospital of the Chinese PLA, Beijing 100091;</nlm:aff><wicri:noCountry code="subfield">Beijing 100091</wicri:noCountry></affiliation></author><author><name sortKey="Chen, Fuxing" sort="Chen, Fuxing" uniqKey="Chen F" first="Fuxing" last="Chen">Fuxing Chen</name><affiliation><nlm:aff id="af3-etm-06-04-1029">The 97th Hospital of the Chinese PLA, Xuzhou, Jiangsu 221004,<country>P.R. China</country></nlm:aff><wicri:noCountry code="nlm country">P.R. China</wicri:noCountry></affiliation></author><author><name sortKey="Liu, Junquan" sort="Liu, Junquan" uniqKey="Liu J" first="Junquan" last="Liu">Junquan Liu</name><affiliation><nlm:aff id="af3-etm-06-04-1029">The 97th Hospital of the Chinese PLA, Xuzhou, Jiangsu 221004,<country>P.R. China</country></nlm:aff><wicri:noCountry code="nlm country">P.R. China</wicri:noCountry></affiliation></author><author><name sortKey="Zhou, Zonghai" sort="Zhou, Zonghai" uniqKey="Zhou Z" first="Zonghai" last="Zhou">Zonghai Zhou</name><affiliation><nlm:aff id="af3-etm-06-04-1029">The 97th Hospital of the Chinese PLA, Xuzhou, Jiangsu 221004,<country>P.R. China</country></nlm:aff><wicri:noCountry code="nlm country">P.R. 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China</country></nlm:aff><wicri:noCountry code="nlm country">P.R. China</wicri:noCountry></affiliation></author><author><name sortKey="Liu, Junquan" sort="Liu, Junquan" uniqKey="Liu J" first="Junquan" last="Liu">Junquan Liu</name><affiliation><nlm:aff id="af3-etm-06-04-1029">The 97th Hospital of the Chinese PLA, Xuzhou, Jiangsu 221004,<country>P.R. China</country></nlm:aff><wicri:noCountry code="nlm country">P.R. China</wicri:noCountry></affiliation></author><author><name sortKey="Zhou, Zonghai" sort="Zhou, Zonghai" uniqKey="Zhou Z" first="Zonghai" last="Zhou">Zonghai Zhou</name><affiliation><nlm:aff id="af3-etm-06-04-1029">The 97th Hospital of the Chinese PLA, Xuzhou, Jiangsu 221004,<country>P.R. China</country></nlm:aff><wicri:noCountry code="nlm country">P.R. China</wicri:noCountry></affiliation></author></analytic><series><title level="j">Experimental and Therapeutic Medicine</title><idno type="ISSN">1792-0981</idno><idno type="eISSN">1792-1015</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The aim of this study was to investigate the effects of heat stress on the expression of the coxsackievirus and adenovirus receptor (CAR) in mouse skin keratinocytes. Twenty BALB/c mice were randomly divided into two groups: the sham heat (control) and scald groups. Skin specimens were obtained 6 h after the treatments. Changes in the expression of CAR in skin keratinocyte samples were detected by immunohistochemistry, quantitative polymerase chain reaction and western blotting. In an <italic>in vitro</italic> assay, mouse skin keratinocytes were cultured and randomly divided into two groups: the normal control and heat stress groups. Six hours subsequently, the changes in CAR expression in the two groups were estimated by flow cytometry to determine the differences between the two groups. Heat stress significantly increased the expression of CAR in the mouse skin keratinocytes (P<0.05). The upregulation of CAR in mouse keratinocytes in burn wounds may be beneficial for restoring healing in organisms.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Birkballe, S" uniqKey="Birkballe S">S Birkballe</name></author><author><name sortKey="Karlsmark, T" uniqKey="Karlsmark T">T Karlsmark</name></author><author><name sortKey="Noerregaard, S" uniqKey="Noerregaard S">S Noerregaard</name></author><author><name sortKey="Gottrup, F" uniqKey="Gottrup F">F Gottrup</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hobizal, Kb" uniqKey="Hobizal K">KB Hobizal</name></author><author><name sortKey="Wukich, Dk" uniqKey="Wukich D">DK Wukich</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Witherden, Da" uniqKey="Witherden D">DA Witherden</name></author><author><name sortKey="Verdino, P" uniqKey="Verdino P">P Verdino</name></author><author><name sortKey="Rieder, Se" 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sortKey="Taylor, Kr" uniqKey="Taylor K">KR Taylor</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Chen, Fuxing" sort="Chen, Fuxing" uniqKey="Chen F" first="Fuxing" last="Chen">Fuxing Chen</name><name sortKey="Deng, Xiangdong" sort="Deng, Xiangdong" uniqKey="Deng X" first="Xiangdong" last="Deng">Xiangdong Deng</name><name sortKey="Jia, Chiyu" sort="Jia, Chiyu" uniqKey="Jia C" first="Chiyu" last="Jia">Chiyu Jia</name><name sortKey="Liu, Junquan" sort="Liu, Junquan" uniqKey="Liu J" first="Junquan" last="Liu">Junquan Liu</name><name sortKey="Zhou, Zonghai" sort="Zhou, Zonghai" uniqKey="Zhou Z" first="Zonghai" last="Zhou">Zonghai Zhou</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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