Effects of heat stress on the expression of the coxsackievirus and adenovirus receptor in mouse skin keratinocytes
Identifieur interne : 002365 ( Pmc/Checkpoint ); précédent : 002364; suivant : 002366Effects of heat stress on the expression of the coxsackievirus and adenovirus receptor in mouse skin keratinocytes
Auteurs : Xiangdong Deng ; Chiyu Jia ; Fuxing Chen ; Junquan Liu ; Zonghai ZhouSource :
- Experimental and Therapeutic Medicine [ 1792-0981 ] ; 2013.
Abstract
The aim of this study was to investigate the effects of heat stress on the expression of the coxsackievirus and adenovirus receptor (CAR) in mouse skin keratinocytes. Twenty BALB/c mice were randomly divided into two groups: the sham heat (control) and scald groups. Skin specimens were obtained 6 h after the treatments. Changes in the expression of CAR in skin keratinocyte samples were detected by immunohistochemistry, quantitative polymerase chain reaction and western blotting. In an
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DOI: 10.3892/etm.2013.1230
PubMed: 24137310
PubMed Central: 3797294
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Effects of heat stress on the expression of the coxsackievirus and adenovirus receptor in mouse skin keratinocytes</title><author><name sortKey="Deng, Xiangdong" sort="Deng, Xiangdong" uniqKey="Deng X" first="Xiangdong" last="Deng">Xiangdong Deng</name><affiliation><nlm:aff id="af1-etm-06-04-1029">The Graduate School, Chinese People’s Liberation Army Medical College, Beijing 100039;</nlm:aff><wicri:noCountry code="subfield">Beijing 100039</wicri:noCountry></affiliation></author><author><name sortKey="Jia, Chiyu" sort="Jia, Chiyu" uniqKey="Jia C" first="Chiyu" last="Jia">Chiyu Jia</name><affiliation><nlm:aff id="af2-etm-06-04-1029">Plastic Beauty and Burn Repair Center, The 309th Hospital of the Chinese PLA, Beijing 100091;</nlm:aff><wicri:noCountry code="subfield">Beijing 100091</wicri:noCountry></affiliation></author><author><name sortKey="Chen, Fuxing" sort="Chen, Fuxing" uniqKey="Chen F" first="Fuxing" last="Chen">Fuxing Chen</name><affiliation><nlm:aff id="af3-etm-06-04-1029">The 97th Hospital of the Chinese PLA, Xuzhou, Jiangsu 221004,<country>P.R. China</country></nlm:aff><wicri:noCountry code="nlm country">P.R. China</wicri:noCountry></affiliation></author><author><name sortKey="Liu, Junquan" sort="Liu, Junquan" uniqKey="Liu J" first="Junquan" last="Liu">Junquan Liu</name><affiliation><nlm:aff id="af3-etm-06-04-1029">The 97th Hospital of the Chinese PLA, Xuzhou, Jiangsu 221004,<country>P.R. China</country></nlm:aff><wicri:noCountry code="nlm country">P.R. China</wicri:noCountry></affiliation></author><author><name sortKey="Zhou, Zonghai" sort="Zhou, Zonghai" uniqKey="Zhou Z" first="Zonghai" last="Zhou">Zonghai Zhou</name><affiliation><nlm:aff id="af3-etm-06-04-1029">The 97th Hospital of the Chinese PLA, Xuzhou, Jiangsu 221004,<country>P.R. China</country></nlm:aff><wicri:noCountry code="nlm country">P.R. China</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24137310</idno><idno type="pmc">3797294</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3797294</idno><idno type="RBID">PMC:3797294</idno><idno type="doi">10.3892/etm.2013.1230</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">003E37</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">003E37</idno><idno type="wicri:Area/Pmc/Curation">003E36</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">003E36</idno><idno type="wicri:Area/Pmc/Checkpoint">002365</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">002365</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Effects of heat stress on the expression of the coxsackievirus and adenovirus receptor in mouse skin keratinocytes</title><author><name sortKey="Deng, Xiangdong" sort="Deng, Xiangdong" uniqKey="Deng X" first="Xiangdong" last="Deng">Xiangdong Deng</name><affiliation><nlm:aff id="af1-etm-06-04-1029">The Graduate School, Chinese People’s Liberation Army Medical College, Beijing 100039;</nlm:aff><wicri:noCountry code="subfield">Beijing 100039</wicri:noCountry></affiliation></author><author><name sortKey="Jia, Chiyu" sort="Jia, Chiyu" uniqKey="Jia C" first="Chiyu" last="Jia">Chiyu Jia</name><affiliation><nlm:aff id="af2-etm-06-04-1029">Plastic Beauty and Burn Repair Center, The 309th Hospital of the Chinese PLA, Beijing 100091;</nlm:aff><wicri:noCountry code="subfield">Beijing 100091</wicri:noCountry></affiliation></author><author><name sortKey="Chen, Fuxing" sort="Chen, Fuxing" uniqKey="Chen F" first="Fuxing" last="Chen">Fuxing Chen</name><affiliation><nlm:aff id="af3-etm-06-04-1029">The 97th Hospital of the Chinese PLA, Xuzhou, Jiangsu 221004,<country>P.R. China</country></nlm:aff><wicri:noCountry code="nlm country">P.R. China</wicri:noCountry></affiliation></author><author><name sortKey="Liu, Junquan" sort="Liu, Junquan" uniqKey="Liu J" first="Junquan" last="Liu">Junquan Liu</name><affiliation><nlm:aff id="af3-etm-06-04-1029">The 97th Hospital of the Chinese PLA, Xuzhou, Jiangsu 221004,<country>P.R. China</country></nlm:aff><wicri:noCountry code="nlm country">P.R. China</wicri:noCountry></affiliation></author><author><name sortKey="Zhou, Zonghai" sort="Zhou, Zonghai" uniqKey="Zhou Z" first="Zonghai" last="Zhou">Zonghai Zhou</name><affiliation><nlm:aff id="af3-etm-06-04-1029">The 97th Hospital of the Chinese PLA, Xuzhou, Jiangsu 221004,<country>P.R. China</country></nlm:aff><wicri:noCountry code="nlm country">P.R. China</wicri:noCountry></affiliation></author></analytic><series><title level="j">Experimental and Therapeutic Medicine</title><idno type="ISSN">1792-0981</idno><idno type="eISSN">1792-1015</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The aim of this study was to investigate the effects of heat stress on the expression of the coxsackievirus and adenovirus receptor (CAR) in mouse skin keratinocytes. Twenty BALB/c mice were randomly divided into two groups: the sham heat (control) and scald groups. Skin specimens were obtained 6 h after the treatments. Changes in the expression of CAR in skin keratinocyte samples were detected by immunohistochemistry, quantitative polymerase chain reaction and western blotting. In an <italic>in vitro</italic> assay, mouse skin keratinocytes were cultured and randomly divided into two groups: the normal control and heat stress groups. Six hours subsequently, the changes in CAR expression in the two groups were estimated by flow cytometry to determine the differences between the two groups. Heat stress significantly increased the expression of CAR in the mouse skin keratinocytes (P<0.05). 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Exp Ther Med</journal-id><journal-id journal-id-type="iso-abbrev">Exp Ther Med</journal-id><journal-id journal-id-type="publisher-id">ETM</journal-id><journal-title-group><journal-title>Experimental and Therapeutic Medicine</journal-title></journal-title-group><issn pub-type="ppub">1792-0981</issn><issn pub-type="epub">1792-1015</issn><publisher><publisher-name>D.A. Spandidos</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24137310</article-id><article-id pub-id-type="pmc">3797294</article-id><article-id pub-id-type="doi">10.3892/etm.2013.1230</article-id><article-id pub-id-type="publisher-id">etm-06-04-1029</article-id><article-categories><subj-group subj-group-type="heading"><subject>Articles</subject></subj-group></article-categories><title-group><article-title>Effects of heat stress on the expression of the coxsackievirus and adenovirus receptor in mouse skin keratinocytes</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>DENG</surname><given-names>XIANGDONG</given-names></name><xref ref-type="aff" rid="af1-etm-06-04-1029"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>JIA</surname><given-names>CHIYU</given-names></name><xref ref-type="aff" rid="af2-etm-06-04-1029"><sup>2</sup></xref><xref ref-type="corresp" rid="c1-etm-06-04-1029"></xref></contrib><contrib contrib-type="author"><name><surname>CHEN</surname><given-names>FUXING</given-names></name><xref ref-type="aff" rid="af3-etm-06-04-1029"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>LIU</surname><given-names>JUNQUAN</given-names></name><xref ref-type="aff" rid="af3-etm-06-04-1029"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>ZHOU</surname><given-names>ZONGHAI</given-names></name><xref ref-type="aff" rid="af3-etm-06-04-1029"><sup>3</sup></xref></contrib></contrib-group><aff id="af1-etm-06-04-1029"><label>1</label>The Graduate School, Chinese People’s Liberation Army Medical College, Beijing 100039;</aff><aff id="af2-etm-06-04-1029"><label>2</label>Plastic Beauty and Burn Repair Center, The 309th Hospital of the Chinese PLA, Beijing 100091;</aff><aff id="af3-etm-06-04-1029"><label>3</label>The 97th Hospital of the Chinese PLA, Xuzhou, Jiangsu 221004,<country>P.R. China</country></aff><author-notes><corresp id="c1-etm-06-04-1029">Correspondence to: Professor Chiyu Jia, Plastic Beauty and Burn Repair Center, 309th Hospital of the Chinese PLA, No.17 Heishanhu, Beijing 100091, P.R. China, E-mail: <email>chiyujiacn@yeah.net</email></corresp></author-notes><pub-date pub-type="ppub"><month>10</month><year>2013</year></pub-date><pub-date pub-type="epub"><day>23</day><month>7</month><year>2013</year></pub-date><pub-date pub-type="pmc-release"><day>23</day><month>7</month><year>2013</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
<volume>6</volume><issue>4</issue><fpage>1029</fpage><lpage>1033</lpage><history><date date-type="received"><day>04</day><month>4</month><year>2013</year></date><date date-type="accepted"><day>15</day><month>7</month><year>2013</year></date></history><permissions><copyright-statement>Copyright © 2013, Spandidos Publications</copyright-statement><copyright-year>2013</copyright-year><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/3.0"><license-p>This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.</license-p></license></permissions><abstract><p>The aim of this study was to investigate the effects of heat stress on the expression of the coxsackievirus and adenovirus receptor (CAR) in mouse skin keratinocytes. Twenty BALB/c mice were randomly divided into two groups: the sham heat (control) and scald groups. Skin specimens were obtained 6 h after the treatments. Changes in the expression of CAR in skin keratinocyte samples were detected by immunohistochemistry, quantitative polymerase chain reaction and western blotting. In an <italic>in vitro</italic> assay, mouse skin keratinocytes were cultured and randomly divided into two groups: the normal control and heat stress groups. Six hours subsequently, the changes in CAR expression in the two groups were estimated by flow cytometry to determine the differences between the two groups. Heat stress significantly increased the expression of CAR in the mouse skin keratinocytes (P<0.05). The upregulation of CAR in mouse keratinocytes in burn wounds may be beneficial for restoring healing in organisms.</p></abstract><kwd-group><kwd>heat stress</kwd><kwd>keratinocytes</kwd><kwd>coxsackie-adenovirus receptor</kwd><kwd>burn wound</kwd></kwd-group></article-meta></front><floats-group><fig id="f1-etm-06-04-1029" orientation="portrait" position="float"><label>Figure 1.</label><caption><p>Histological observations. (A) Blank control; (B) epidermal keratinocytes of the normal mouse skin; (C) mouse skin epidermis following thermal stimulation. Immunostaining was used. The arrows indicate the skin epidermal keratinocytes where CAR may be expressed. Magnification, ×40.</p></caption><graphic xlink:href="ETM-06-04-1029-g00"></graphic></fig><fig id="f2-etm-06-04-1029" orientation="portrait" position="float"><label>Figure 2.</label><caption><p>Effect of heat stress on the relative mRNA expression levels of the coxsackievirus and adenovirus receptor (CAR) in mouse keratinocytes.</p></caption><graphic xlink:href="ETM-06-04-1029-g01"></graphic></fig><fig id="f3-etm-06-04-1029" orientation="portrait" position="float"><label>Figure 3.</label><caption><p>Relative expression of the coxsackievirus and adenovirus receptor (CAR) protein in heat-stressed (test) and normal mouse keratinocytes.</p></caption><graphic xlink:href="ETM-06-04-1029-g02"></graphic></fig><fig id="f4-etm-06-04-1029" orientation="portrait" position="float"><label>Figure 4.</label><caption><p>Detection of: (A) normal keratinocyte apoptosis; (B) keratinocyte apoptosis under heat stress.</p></caption><graphic xlink:href="ETM-06-04-1029-g03"></graphic></fig><fig id="f5-etm-06-04-1029" orientation="portrait" position="float"><label>Figure 5.</label><caption><p>Expression of coxsackie-adenovirus receptor (CAR) in: (A) normal keratinocytes and (B) normal keratinocytes under heat stress. (C) CAR expression changes in keratinocytes under heat stress. FITC, fluorescein isothiocyanate.</p></caption><graphic xlink:href="ETM-06-04-1029-g04"></graphic></fig><table-wrap id="t1-etm-06-04-1029" orientation="portrait" position="float"><label>Table I.</label><caption><p>Relative quantitative expression of CAR gene.</p></caption><table frame="hsides" rules="groups"><thead><tr><th align="left" valign="middle" rowspan="1" colspan="1">Group</th><th align="center" valign="middle" rowspan="1" colspan="1">CAR Ct</th><th align="center" valign="middle" rowspan="1" colspan="1">GAPDH Ct</th><th align="center" valign="middle" rowspan="1" colspan="1">E-<sup>ΔΔCt</sup></th></tr></thead><tbody><tr><td align="left" valign="top" rowspan="1" colspan="1">Normal mice</td><td align="center" valign="top" rowspan="1" colspan="1">20.69±0.106</td><td align="center" valign="top" rowspan="1" colspan="1">15.60±0.220</td><td align="center" valign="top" rowspan="1" colspan="1">0.157±0.027</td></tr><tr><td align="left" valign="top" rowspan="1" colspan="1">Scalded mice</td><td align="center" valign="top" rowspan="1" colspan="1">19.89±0.018</td><td align="center" valign="top" rowspan="1" colspan="1">16.98±0.048</td><td align="center" valign="top" rowspan="1" colspan="1">0.773±0.029<xref rid="tfn2-etm-06-04-1029" ref-type="table-fn"><sup>a</sup></xref></td></tr></tbody></table><table-wrap-foot><fn id="tfn1-etm-06-04-1029"><p>n=10 per group;</p></fn><fn id="tfn2-etm-06-04-1029"><label>a</label><p>P<0.05 compared with normal mice. CAR, coxsackievirus and adenovirus receptor; GADPH, glyceraldehyde 3-phosphate dehydrogenase.</p></fn></table-wrap-foot></table-wrap><table-wrap id="t2-etm-06-04-1029" orientation="portrait" position="float"><label>Table II.</label><caption><p>Relative grey value of CAR protein.</p></caption><table frame="hsides" rules="groups"><thead><tr><th align="left" valign="middle" rowspan="1" colspan="1">Group</th><th align="center" valign="middle" rowspan="1" colspan="1">n</th><th align="center" valign="middle" rowspan="1" colspan="1">CAR/GAPDH</th></tr></thead><tbody><tr><td align="left" valign="top" rowspan="1" colspan="1">Normal mice</td><td align="center" valign="top" rowspan="1" colspan="1">10</td><td align="center" valign="top" rowspan="1" colspan="1">0.227±0.093</td></tr><tr><td align="left" valign="top" rowspan="1" colspan="1">Scalded mice</td><td align="center" valign="top" rowspan="1" colspan="1">10</td><td align="center" valign="top" rowspan="1" colspan="1">0.891±0.144<xref rid="tfn4-etm-06-04-1029" ref-type="table-fn"><sup>a</sup></xref></td></tr></tbody></table><table-wrap-foot><fn id="tfn3-etm-06-04-1029"><p>Results are presented as the mean ± standard deviation.</p></fn><fn id="tfn4-etm-06-04-1029"><label>a</label><p>P<0.05 compared with normal mice. CAR, coxsackie virus and adenovirus receptor; GADPH, glyceraldehyde 3-phosphate dehydrogenase.</p></fn></table-wrap-foot></table-wrap><table-wrap id="t3-etm-06-04-1029" orientation="portrait" position="float"><label>Table III.</label><caption><p>Expression of CAR in keratinocytes detected using flow cytometry (mean ± SD)%.</p></caption><table frame="hsides" rules="groups"><thead><tr><th align="left" valign="middle" rowspan="1" colspan="1">Group</th><th align="center" valign="middle" rowspan="1" colspan="1">n</th><th align="center" valign="middle" rowspan="1" colspan="1">CAR expression</th></tr></thead><tbody><tr><td align="left" valign="bottom" rowspan="1" colspan="1">Normal keratinocytes</td><td align="center" valign="bottom" rowspan="1" colspan="1">12</td><td align="center" valign="bottom" rowspan="1" colspan="1">48.36±5.07</td></tr><tr><td align="left" valign="bottom" rowspan="1" colspan="1">Keratinocytes after thermal stimulation</td><td align="center" valign="bottom" rowspan="1" colspan="1">12</td><td align="center" valign="bottom" rowspan="1" colspan="1">78.64±7.96<xref rid="tfn5-etm-06-04-1029" ref-type="table-fn"><sup>a</sup></xref></td></tr></tbody></table><table-wrap-foot><fn id="tfn5-etm-06-04-1029"><label>a</label><p>P<0.05 compared with normal keratinocytes. CAR, coxsackie-adenovirus receptor; SD, standard deviation.</p></fn></table-wrap-foot></table-wrap></floats-group></pmc><affiliations><list></list><tree><noCountry><name sortKey="Chen, Fuxing" sort="Chen, Fuxing" uniqKey="Chen F" first="Fuxing" last="Chen">Fuxing Chen</name><name sortKey="Deng, Xiangdong" sort="Deng, Xiangdong" uniqKey="Deng X" first="Xiangdong" last="Deng">Xiangdong Deng</name><name sortKey="Jia, Chiyu" sort="Jia, Chiyu" uniqKey="Jia C" first="Chiyu" last="Jia">Chiyu Jia</name><name sortKey="Liu, Junquan" sort="Liu, Junquan" uniqKey="Liu J" first="Junquan" last="Liu">Junquan Liu</name><name sortKey="Zhou, Zonghai" sort="Zhou, Zonghai" uniqKey="Zhou Z" first="Zonghai" last="Zhou">Zonghai Zhou</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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