Protective Immune Responses to Biolistic DNA Vaccination of Brugia malayi Abundant Larval Transcript -2
Identifieur interne : 005034 ( Ncbi/Checkpoint ); précédent : 005033; suivant : 005035Protective Immune Responses to Biolistic DNA Vaccination of Brugia malayi Abundant Larval Transcript -2
Auteurs : Sk Joseph [États-Unis] ; S. Sambanthamoorthy [États-Unis] ; G. Dakshinamoorthy [États-Unis] ; G. Munirathinam [États-Unis] ; K. Ramaswamy [États-Unis]Source :
- Vaccine [ 0264-410X ] ; 2012.
Descripteurs français
- KwdFr :
- ADN des helminthes (immunologie), Animaux, Anticorps antihelminthe (immunologie), Antigènes d'helminthe (génétique), Antigènes d'helminthe (immunologie), Biolistique (), Cellules COS, Cercopithecus aethiops, Facteur de nécrose tumorale alpha (immunologie), Filariose lymphatique (), Immunoglobuline G (immunologie), Injections intradermiques, Interféron gamma (immunologie), Interleukine-12 (immunologie), Interleukine-4 (immunologie), Protéines recombinantes (génétique), Protéines recombinantes (immunologie), Souris, Souris de lignée BALB C, Vaccins (immunologie), Vaccins à ADN (immunologie).
- MESH :
- génétique : Antigènes d'helminthe, Protéines recombinantes.
- immunologie : ADN des helminthes, Anticorps antihelminthe, Antigènes d'helminthe, Facteur de nécrose tumorale alpha, Immunoglobuline G, Interféron gamma, Interleukine-12, Interleukine-4, Protéines recombinantes, Vaccins, Vaccins à ADN.
- Animaux, Biolistique, Cellules COS, Cercopithecus aethiops, Filariose lymphatique, Injections intradermiques, Souris, Souris de lignée BALB C.
English descriptors
- KwdEn :
- Animals, Antibodies, Helminth (immunology), Antigens, Helminth (genetics), Antigens, Helminth (immunology), Biolistics (methods), COS Cells, Cercopithecus aethiops, DNA, Helminth (immunology), Elephantiasis, Filarial (prevention & control), Immunoglobulin G (immunology), Injections, Intradermal, Interferon-gamma (immunology), Interleukin-12 (immunology), Interleukin-4 (immunology), Mice, Mice, Inbred BALB C, Recombinant Proteins (genetics), Recombinant Proteins (immunology), Tumor Necrosis Factor-alpha (immunology), Vaccines (immunology), Vaccines, DNA (immunology).
- MESH :
- chemical , genetics : Antigens, Helminth, Recombinant Proteins.
- chemical , immunology : Antibodies, Helminth, Antigens, Helminth, DNA, Helminth, Immunoglobulin G, Interferon-gamma, Interleukin-12, Interleukin-4, Recombinant Proteins, Tumor Necrosis Factor-alpha, Vaccines, Vaccines, DNA.
- methods : Biolistics.
- prevention & control : Elephantiasis, Filarial.
- Animals, COS Cells, Cercopithecus aethiops, Injections, Intradermal, Mice, Mice, Inbred BALB C.
Abstract
Biolistic vaccination using gene gun is developed as a safer tool for delivery of DNA vaccines, a technique that combines high vaccine efficiency with lower antigen dosage and lower cost per vaccine dose. In this study, we compared the protective responses in mice after delivering the
Url:
DOI: 10.1016/j.vaccine.2012.07.084
PubMed: 22885273
PubMed Central: 3467021
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 003793
- to stream Pmc, to step Curation: 003792
- to stream Pmc, to step Checkpoint: 002790
- to stream PubMed, to step Corpus: 002000
- to stream PubMed, to step Curation: 002000
- to stream PubMed, to step Checkpoint: 002000
- to stream Ncbi, to step Merge: 005034
- to stream Ncbi, to step Curation: 005034
Links to Exploration step
PMC:3467021Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Protective Immune Responses to Biolistic DNA Vaccination of <italic>Brugia malayi</italic>
Abundant Larval Transcript -2</title>
<author><name sortKey="Joseph, Sk" sort="Joseph, Sk" uniqKey="Joseph S" first="Sk" last="Joseph">Sk Joseph</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford</wicri:regionArea>
<wicri:noRegion>Rockford</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sambanthamoorthy, S" sort="Sambanthamoorthy, S" uniqKey="Sambanthamoorthy S" first="S" last="Sambanthamoorthy">S. Sambanthamoorthy</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford</wicri:regionArea>
<wicri:noRegion>Rockford</wicri:noRegion>
</affiliation>
<affiliation wicri:level="3"><nlm:aff id="A2">Harvard Medical School, Boston, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Harvard Medical School, Boston</wicri:regionArea>
<placeName><settlement type="city">Boston</settlement>
<region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Dakshinamoorthy, G" sort="Dakshinamoorthy, G" uniqKey="Dakshinamoorthy G" first="G" last="Dakshinamoorthy">G. Dakshinamoorthy</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford</wicri:regionArea>
<wicri:noRegion>Rockford</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Munirathinam, G" sort="Munirathinam, G" uniqKey="Munirathinam G" first="G" last="Munirathinam">G. Munirathinam</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford</wicri:regionArea>
<wicri:noRegion>Rockford</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ramaswamy, K" sort="Ramaswamy, K" uniqKey="Ramaswamy K" first="K" last="Ramaswamy">K. Ramaswamy</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford</wicri:regionArea>
<wicri:noRegion>Rockford</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">22885273</idno>
<idno type="pmc">3467021</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3467021</idno>
<idno type="RBID">PMC:3467021</idno>
<idno type="doi">10.1016/j.vaccine.2012.07.084</idno>
<date when="2012">2012</date>
<idno type="wicri:Area/Pmc/Corpus">003793</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">003793</idno>
<idno type="wicri:Area/Pmc/Curation">003792</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">003792</idno>
<idno type="wicri:Area/Pmc/Checkpoint">002790</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">002790</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="wicri:Area/PubMed/Corpus">002000</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002000</idno>
<idno type="wicri:Area/PubMed/Curation">002000</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002000</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002000</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002000</idno>
<idno type="wicri:Area/Ncbi/Merge">005034</idno>
<idno type="wicri:Area/Ncbi/Curation">005034</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">005034</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Protective Immune Responses to Biolistic DNA Vaccination of <italic>Brugia malayi</italic>
Abundant Larval Transcript -2</title>
<author><name sortKey="Joseph, Sk" sort="Joseph, Sk" uniqKey="Joseph S" first="Sk" last="Joseph">Sk Joseph</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford</wicri:regionArea>
<wicri:noRegion>Rockford</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sambanthamoorthy, S" sort="Sambanthamoorthy, S" uniqKey="Sambanthamoorthy S" first="S" last="Sambanthamoorthy">S. Sambanthamoorthy</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford</wicri:regionArea>
<wicri:noRegion>Rockford</wicri:noRegion>
</affiliation>
<affiliation wicri:level="3"><nlm:aff id="A2">Harvard Medical School, Boston, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Harvard Medical School, Boston</wicri:regionArea>
<placeName><settlement type="city">Boston</settlement>
<region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Dakshinamoorthy, G" sort="Dakshinamoorthy, G" uniqKey="Dakshinamoorthy G" first="G" last="Dakshinamoorthy">G. Dakshinamoorthy</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford</wicri:regionArea>
<wicri:noRegion>Rockford</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Munirathinam, G" sort="Munirathinam, G" uniqKey="Munirathinam G" first="G" last="Munirathinam">G. Munirathinam</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford</wicri:regionArea>
<wicri:noRegion>Rockford</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ramaswamy, K" sort="Ramaswamy, K" uniqKey="Ramaswamy K" first="K" last="Ramaswamy">K. Ramaswamy</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford</wicri:regionArea>
<wicri:noRegion>Rockford</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Vaccine</title>
<idno type="ISSN">0264-410X</idno>
<idno type="eISSN">1873-2518</idno>
<imprint><date when="2012">2012</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Antibodies, Helminth (immunology)</term>
<term>Antigens, Helminth (genetics)</term>
<term>Antigens, Helminth (immunology)</term>
<term>Biolistics (methods)</term>
<term>COS Cells</term>
<term>Cercopithecus aethiops</term>
<term>DNA, Helminth (immunology)</term>
<term>Elephantiasis, Filarial (prevention & control)</term>
<term>Immunoglobulin G (immunology)</term>
<term>Injections, Intradermal</term>
<term>Interferon-gamma (immunology)</term>
<term>Interleukin-12 (immunology)</term>
<term>Interleukin-4 (immunology)</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Recombinant Proteins (genetics)</term>
<term>Recombinant Proteins (immunology)</term>
<term>Tumor Necrosis Factor-alpha (immunology)</term>
<term>Vaccines (immunology)</term>
<term>Vaccines, DNA (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN des helminthes (immunologie)</term>
<term>Animaux</term>
<term>Anticorps antihelminthe (immunologie)</term>
<term>Antigènes d'helminthe (génétique)</term>
<term>Antigènes d'helminthe (immunologie)</term>
<term>Biolistique ()</term>
<term>Cellules COS</term>
<term>Cercopithecus aethiops</term>
<term>Facteur de nécrose tumorale alpha (immunologie)</term>
<term>Filariose lymphatique ()</term>
<term>Immunoglobuline G (immunologie)</term>
<term>Injections intradermiques</term>
<term>Interféron gamma (immunologie)</term>
<term>Interleukine-12 (immunologie)</term>
<term>Interleukine-4 (immunologie)</term>
<term>Protéines recombinantes (génétique)</term>
<term>Protéines recombinantes (immunologie)</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Vaccins (immunologie)</term>
<term>Vaccins à ADN (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Helminth</term>
<term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Helminth</term>
<term>Antigens, Helminth</term>
<term>DNA, Helminth</term>
<term>Immunoglobulin G</term>
<term>Interferon-gamma</term>
<term>Interleukin-12</term>
<term>Interleukin-4</term>
<term>Recombinant Proteins</term>
<term>Tumor Necrosis Factor-alpha</term>
<term>Vaccines</term>
<term>Vaccines, DNA</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Antigènes d'helminthe</term>
<term>Protéines recombinantes</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>ADN des helminthes</term>
<term>Anticorps antihelminthe</term>
<term>Antigènes d'helminthe</term>
<term>Facteur de nécrose tumorale alpha</term>
<term>Immunoglobuline G</term>
<term>Interféron gamma</term>
<term>Interleukine-12</term>
<term>Interleukine-4</term>
<term>Protéines recombinantes</term>
<term>Vaccins</term>
<term>Vaccins à ADN</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biolistics</term>
</keywords>
<keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Elephantiasis, Filarial</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>COS Cells</term>
<term>Cercopithecus aethiops</term>
<term>Injections, Intradermal</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Biolistique</term>
<term>Cellules COS</term>
<term>Cercopithecus aethiops</term>
<term>Filariose lymphatique</term>
<term>Injections intradermiques</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p id="P1">Biolistic vaccination using gene gun is developed as a safer tool for delivery of DNA vaccines, a technique that combines high vaccine efficiency with lower antigen dosage and lower cost per vaccine dose. In this study, we compared the protective responses in mice after delivering the <italic>BmALT-2</italic>
DNA vaccine using the conventional intradermal approach or with the needleless gene gun delivery approach. <italic>BmALT-2</italic>
is a leading vaccine candidate against <italic>Brugia malayi</italic>
, a lymphatic filarial parasite of human. After optimizing the DNA dose and gene gun parameters for delivery into mouse skin, groups of mice were biolistically vaccinated with 5µg of <italic>BmALT-2pVAX</italic>
. Groups of mice vaccinated intradermally with 5µg or 100µg of <italic>BmALT-2pVAX</italic>
was used for comparison of vaccine efficacy. Results demonstrated that gene gun vaccination with 5µg of <italic>BmALT-2pVAX</italic>
conferred significant protection against challenge infection that was comparable to the degree of protection conferred by intradermal vaccination with 100µg of <italic>BmALT-2pVAX</italic>
. This observation was further supported by an <italic>in vitro</italic>
antibody dependent cellular cytotoxicity (ADCC) assay. Analysis of the immune response showed that the gene gun vaccination predominantly induced an IgG1 antibody response and significantly high Th2 cytokine response (IL-4) from spleen cells compared to intradermal <italic>BmALT-2</italic>
DNA delivery that induced predominantly an IgG2a and Th1 cytokine response (IFN-γ, IL-12 and TNF-α). These findings show that host protective responses could be achieved with 20 fold decrease in DNA dose using a gene gun and could prove to be an efficient delivery method in <italic>BmALT-2</italic>
DNA vaccination against lymphatic filariasis.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Massachusetts</li>
</region>
<settlement><li>Boston</li>
</settlement>
</list>
<tree><country name="États-Unis"><noRegion><name sortKey="Joseph, Sk" sort="Joseph, Sk" uniqKey="Joseph S" first="Sk" last="Joseph">Sk Joseph</name>
</noRegion>
<name sortKey="Dakshinamoorthy, G" sort="Dakshinamoorthy, G" uniqKey="Dakshinamoorthy G" first="G" last="Dakshinamoorthy">G. Dakshinamoorthy</name>
<name sortKey="Munirathinam, G" sort="Munirathinam, G" uniqKey="Munirathinam G" first="G" last="Munirathinam">G. Munirathinam</name>
<name sortKey="Ramaswamy, K" sort="Ramaswamy, K" uniqKey="Ramaswamy K" first="K" last="Ramaswamy">K. Ramaswamy</name>
<name sortKey="Sambanthamoorthy, S" sort="Sambanthamoorthy, S" uniqKey="Sambanthamoorthy S" first="S" last="Sambanthamoorthy">S. Sambanthamoorthy</name>
<name sortKey="Sambanthamoorthy, S" sort="Sambanthamoorthy, S" uniqKey="Sambanthamoorthy S" first="S" last="Sambanthamoorthy">S. Sambanthamoorthy</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Ncbi/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 005034 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd -nk 005034 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= LymphedemaV1 |flux= Ncbi |étape= Checkpoint |type= RBID |clé= PMC:3467021 |texte= Protective Immune Responses to Biolistic DNA Vaccination of Brugia malayi Abundant Larval Transcript -2 }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/RBID.i -Sk "pubmed:22885273" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a LymphedemaV1
This area was generated with Dilib version V0.6.31. |