Protective Immune Responses to Biolistic DNA Vaccination of Brugia malayi Abundant Larval Transcript -2
Identifieur interne : 005034 ( Ncbi/Merge ); précédent : 005033; suivant : 005035Protective Immune Responses to Biolistic DNA Vaccination of Brugia malayi Abundant Larval Transcript -2
Auteurs : Sk Joseph [États-Unis] ; S. Sambanthamoorthy [États-Unis] ; G. Dakshinamoorthy [États-Unis] ; G. Munirathinam [États-Unis] ; K. Ramaswamy [États-Unis]Source :
- Vaccine [ 0264-410X ] ; 2012.
Descripteurs français
- KwdFr :
- ADN des helminthes (immunologie), Animaux, Anticorps antihelminthe (immunologie), Antigènes d'helminthe (génétique), Antigènes d'helminthe (immunologie), Biolistique (), Cellules COS, Cercopithecus aethiops, Facteur de nécrose tumorale alpha (immunologie), Filariose lymphatique (), Immunoglobuline G (immunologie), Injections intradermiques, Interféron gamma (immunologie), Interleukine-12 (immunologie), Interleukine-4 (immunologie), Protéines recombinantes (génétique), Protéines recombinantes (immunologie), Souris, Souris de lignée BALB C, Vaccins (immunologie), Vaccins à ADN (immunologie).
- MESH :
- génétique : Antigènes d'helminthe, Protéines recombinantes.
- immunologie : ADN des helminthes, Anticorps antihelminthe, Antigènes d'helminthe, Facteur de nécrose tumorale alpha, Immunoglobuline G, Interféron gamma, Interleukine-12, Interleukine-4, Protéines recombinantes, Vaccins, Vaccins à ADN.
- Animaux, Biolistique, Cellules COS, Cercopithecus aethiops, Filariose lymphatique, Injections intradermiques, Souris, Souris de lignée BALB C.
English descriptors
- KwdEn :
- Animals, Antibodies, Helminth (immunology), Antigens, Helminth (genetics), Antigens, Helminth (immunology), Biolistics (methods), COS Cells, Cercopithecus aethiops, DNA, Helminth (immunology), Elephantiasis, Filarial (prevention & control), Immunoglobulin G (immunology), Injections, Intradermal, Interferon-gamma (immunology), Interleukin-12 (immunology), Interleukin-4 (immunology), Mice, Mice, Inbred BALB C, Recombinant Proteins (genetics), Recombinant Proteins (immunology), Tumor Necrosis Factor-alpha (immunology), Vaccines (immunology), Vaccines, DNA (immunology).
- MESH :
- chemical , genetics : Antigens, Helminth, Recombinant Proteins.
- chemical , immunology : Antibodies, Helminth, Antigens, Helminth, DNA, Helminth, Immunoglobulin G, Interferon-gamma, Interleukin-12, Interleukin-4, Recombinant Proteins, Tumor Necrosis Factor-alpha, Vaccines, Vaccines, DNA.
- methods : Biolistics.
- prevention & control : Elephantiasis, Filarial.
- Animals, COS Cells, Cercopithecus aethiops, Injections, Intradermal, Mice, Mice, Inbred BALB C.
Abstract
Biolistic vaccination using gene gun is developed as a safer tool for delivery of DNA vaccines, a technique that combines high vaccine efficiency with lower antigen dosage and lower cost per vaccine dose. In this study, we compared the protective responses in mice after delivering the
Url:
DOI: 10.1016/j.vaccine.2012.07.084
PubMed: 22885273
PubMed Central: 3467021
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PMC:3467021Le document en format XML
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Munirathinam</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford</wicri:regionArea><wicri:noRegion>Rockford</wicri:noRegion></affiliation></author><author><name sortKey="Ramaswamy, K" sort="Ramaswamy, K" uniqKey="Ramaswamy K" first="K" last="Ramaswamy">K. Ramaswamy</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford</wicri:regionArea><wicri:noRegion>Rockford</wicri:noRegion></affiliation></author></analytic><series><title level="j">Vaccine</title><idno type="ISSN">0264-410X</idno><idno type="eISSN">1873-2518</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Helminth (immunology)</term><term>Antigens, Helminth (genetics)</term><term>Antigens, Helminth (immunology)</term><term>Biolistics (methods)</term><term>COS Cells</term><term>Cercopithecus aethiops</term><term>DNA, Helminth (immunology)</term><term>Elephantiasis, Filarial (prevention & control)</term><term>Immunoglobulin G (immunology)</term><term>Injections, Intradermal</term><term>Interferon-gamma (immunology)</term><term>Interleukin-12 (immunology)</term><term>Interleukin-4 (immunology)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (immunology)</term><term>Tumor Necrosis Factor-alpha (immunology)</term><term>Vaccines (immunology)</term><term>Vaccines, DNA (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN des helminthes (immunologie)</term><term>Animaux</term><term>Anticorps antihelminthe (immunologie)</term><term>Antigènes d'helminthe (génétique)</term><term>Antigènes d'helminthe (immunologie)</term><term>Biolistique ()</term><term>Cellules COS</term><term>Cercopithecus aethiops</term><term>Facteur de nécrose tumorale alpha (immunologie)</term><term>Filariose lymphatique ()</term><term>Immunoglobuline G (immunologie)</term><term>Injections intradermiques</term><term>Interféron gamma (immunologie)</term><term>Interleukine-12 (immunologie)</term><term>Interleukine-4 (immunologie)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (immunologie)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Vaccins (immunologie)</term><term>Vaccins à ADN (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Helminth</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Helminth</term><term>Antigens, Helminth</term><term>DNA, Helminth</term><term>Immunoglobulin G</term><term>Interferon-gamma</term><term>Interleukin-12</term><term>Interleukin-4</term><term>Recombinant Proteins</term><term>Tumor Necrosis Factor-alpha</term><term>Vaccines</term><term>Vaccines, DNA</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Antigènes d'helminthe</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>ADN des helminthes</term><term>Anticorps antihelminthe</term><term>Antigènes d'helminthe</term><term>Facteur de nécrose tumorale alpha</term><term>Immunoglobuline G</term><term>Interféron gamma</term><term>Interleukine-12</term><term>Interleukine-4</term><term>Protéines recombinantes</term><term>Vaccins</term><term>Vaccins à ADN</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biolistics</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>COS Cells</term><term>Cercopithecus aethiops</term><term>Injections, Intradermal</term><term>Mice</term><term>Mice, Inbred BALB C</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Biolistique</term><term>Cellules COS</term><term>Cercopithecus aethiops</term><term>Filariose lymphatique</term><term>Injections intradermiques</term><term>Souris</term><term>Souris de lignée BALB C</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">Biolistic vaccination using gene gun is developed as a safer tool for delivery of DNA vaccines, a technique that combines high vaccine efficiency with lower antigen dosage and lower cost per vaccine dose. In this study, we compared the protective responses in mice after delivering the <italic>BmALT-2</italic> DNA vaccine using the conventional intradermal approach or with the needleless gene gun delivery approach. <italic>BmALT-2</italic> is a leading vaccine candidate against <italic>Brugia malayi</italic>, a lymphatic filarial parasite of human. After optimizing the DNA dose and gene gun parameters for delivery into mouse skin, groups of mice were biolistically vaccinated with 5µg of <italic>BmALT-2pVAX</italic>. Groups of mice vaccinated intradermally with 5µg or 100µg of <italic>BmALT-2pVAX</italic> was used for comparison of vaccine efficacy. Results demonstrated that gene gun vaccination with 5µg of <italic>BmALT-2pVAX</italic> conferred significant protection against challenge infection that was comparable to the degree of protection conferred by intradermal vaccination with 100µg of <italic>BmALT-2pVAX</italic>. This observation was further supported by an <italic>in vitro</italic> antibody dependent cellular cytotoxicity (ADCC) assay. Analysis of the immune response showed that the gene gun vaccination predominantly induced an IgG1 antibody response and significantly high Th2 cytokine response (IL-4) from spleen cells compared to intradermal <italic>BmALT-2</italic> DNA delivery that induced predominantly an IgG2a and Th1 cytokine response (IFN-γ, IL-12 and TNF-α). These findings show that host protective responses could be achieved with 20 fold decrease in DNA dose using a gene gun and could prove to be an efficient delivery method in <italic>BmALT-2</italic> DNA vaccination against lymphatic filariasis.</p></div></front></TEI><double pmid="22885273"><pmc><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Protective Immune Responses to Biolistic DNA Vaccination of <italic>Brugia malayi</italic> Abundant Larval Transcript -2</title><author><name sortKey="Joseph, Sk" sort="Joseph, Sk" uniqKey="Joseph S" first="Sk" last="Joseph">Sk Joseph</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford</wicri:regionArea><wicri:noRegion>Rockford</wicri:noRegion></affiliation></author><author><name sortKey="Sambanthamoorthy, S" sort="Sambanthamoorthy, S" uniqKey="Sambanthamoorthy S" first="S" last="Sambanthamoorthy">S. Sambanthamoorthy</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford</wicri:regionArea><wicri:noRegion>Rockford</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:aff id="A2">Harvard Medical School, Boston, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Harvard Medical School, Boston</wicri:regionArea><placeName><settlement type="city">Boston</settlement><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Dakshinamoorthy, G" sort="Dakshinamoorthy, G" uniqKey="Dakshinamoorthy G" first="G" last="Dakshinamoorthy">G. Dakshinamoorthy</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford</wicri:regionArea><wicri:noRegion>Rockford</wicri:noRegion></affiliation></author><author><name sortKey="Munirathinam, G" sort="Munirathinam, G" uniqKey="Munirathinam G" first="G" last="Munirathinam">G. Munirathinam</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford</wicri:regionArea><wicri:noRegion>Rockford</wicri:noRegion></affiliation></author><author><name sortKey="Ramaswamy, K" sort="Ramaswamy, K" uniqKey="Ramaswamy K" first="K" last="Ramaswamy">K. Ramaswamy</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford</wicri:regionArea><wicri:noRegion>Rockford</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">22885273</idno><idno type="pmc">3467021</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3467021</idno><idno type="RBID">PMC:3467021</idno><idno type="doi">10.1016/j.vaccine.2012.07.084</idno><date when="2012">2012</date><idno type="wicri:Area/Pmc/Corpus">003793</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">003793</idno><idno type="wicri:Area/Pmc/Curation">003792</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">003792</idno><idno type="wicri:Area/Pmc/Checkpoint">002790</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">002790</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Protective Immune Responses to Biolistic DNA Vaccination of <italic>Brugia malayi</italic> Abundant Larval Transcript -2</title><author><name sortKey="Joseph, Sk" sort="Joseph, Sk" uniqKey="Joseph S" first="Sk" last="Joseph">Sk Joseph</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford</wicri:regionArea><wicri:noRegion>Rockford</wicri:noRegion></affiliation></author><author><name sortKey="Sambanthamoorthy, S" sort="Sambanthamoorthy, S" uniqKey="Sambanthamoorthy S" first="S" last="Sambanthamoorthy">S. Sambanthamoorthy</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford</wicri:regionArea><wicri:noRegion>Rockford</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:aff id="A2">Harvard Medical School, Boston, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Harvard Medical School, Boston</wicri:regionArea><placeName><settlement type="city">Boston</settlement><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Dakshinamoorthy, G" sort="Dakshinamoorthy, G" uniqKey="Dakshinamoorthy G" first="G" last="Dakshinamoorthy">G. Dakshinamoorthy</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford</wicri:regionArea><wicri:noRegion>Rockford</wicri:noRegion></affiliation></author><author><name sortKey="Munirathinam, G" sort="Munirathinam, G" uniqKey="Munirathinam G" first="G" last="Munirathinam">G. Munirathinam</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford</wicri:regionArea><wicri:noRegion>Rockford</wicri:noRegion></affiliation></author><author><name sortKey="Ramaswamy, K" sort="Ramaswamy, K" uniqKey="Ramaswamy K" first="K" last="Ramaswamy">K. Ramaswamy</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford</wicri:regionArea><wicri:noRegion>Rockford</wicri:noRegion></affiliation></author></analytic><series><title level="j">Vaccine</title><idno type="ISSN">0264-410X</idno><idno type="eISSN">1873-2518</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">Biolistic vaccination using gene gun is developed as a safer tool for delivery of DNA vaccines, a technique that combines high vaccine efficiency with lower antigen dosage and lower cost per vaccine dose. In this study, we compared the protective responses in mice after delivering the <italic>BmALT-2</italic> DNA vaccine using the conventional intradermal approach or with the needleless gene gun delivery approach. <italic>BmALT-2</italic> is a leading vaccine candidate against <italic>Brugia malayi</italic>, a lymphatic filarial parasite of human. After optimizing the DNA dose and gene gun parameters for delivery into mouse skin, groups of mice were biolistically vaccinated with 5µg of <italic>BmALT-2pVAX</italic>. Groups of mice vaccinated intradermally with 5µg or 100µg of <italic>BmALT-2pVAX</italic> was used for comparison of vaccine efficacy. Results demonstrated that gene gun vaccination with 5µg of <italic>BmALT-2pVAX</italic> conferred significant protection against challenge infection that was comparable to the degree of protection conferred by intradermal vaccination with 100µg of <italic>BmALT-2pVAX</italic>. This observation was further supported by an <italic>in vitro</italic> antibody dependent cellular cytotoxicity (ADCC) assay. Analysis of the immune response showed that the gene gun vaccination predominantly induced an IgG1 antibody response and significantly high Th2 cytokine response (IL-4) from spleen cells compared to intradermal <italic>BmALT-2</italic> DNA delivery that induced predominantly an IgG2a and Th1 cytokine response (IFN-γ, IL-12 and TNF-α). These findings show that host protective responses could be achieved with 20 fold decrease in DNA dose using a gene gun and could prove to be an efficient delivery method in <italic>BmALT-2</italic> DNA vaccination against lymphatic filariasis.</p></div></front></TEI></pmc><pubmed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Protective immune responses to biolistic DNA vaccination of Brugia malayi abundant larval transcript-2.</title><author><name sortKey="Joseph, S K" sort="Joseph, S K" uniqKey="Joseph S" first="S K" last="Joseph">S K Joseph</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, IL, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, IL</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation></author><author><name sortKey="Sambanthamoorthy, S" sort="Sambanthamoorthy, S" uniqKey="Sambanthamoorthy S" first="S" last="Sambanthamoorthy">S. Sambanthamoorthy</name></author><author><name sortKey="Dakshinamoorthy, G" sort="Dakshinamoorthy, G" uniqKey="Dakshinamoorthy G" first="G" last="Dakshinamoorthy">G. Dakshinamoorthy</name></author><author><name sortKey="Munirathinam, G" sort="Munirathinam, G" uniqKey="Munirathinam G" first="G" last="Munirathinam">G. Munirathinam</name></author><author><name sortKey="Ramaswamy, K" sort="Ramaswamy, K" uniqKey="Ramaswamy K" first="K" last="Ramaswamy">K. Ramaswamy</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22885273</idno><idno type="pmid">22885273</idno><idno type="doi">10.1016/j.vaccine.2012.07.084</idno><idno type="wicri:Area/PubMed/Corpus">002000</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002000</idno><idno type="wicri:Area/PubMed/Curation">002000</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002000</idno><idno type="wicri:Area/PubMed/Checkpoint">002000</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002000</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Protective immune responses to biolistic DNA vaccination of Brugia malayi abundant larval transcript-2.</title><author><name sortKey="Joseph, S K" sort="Joseph, S K" uniqKey="Joseph S" first="S K" last="Joseph">S K Joseph</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, IL, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, Rockford, IL</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation></author><author><name sortKey="Sambanthamoorthy, S" sort="Sambanthamoorthy, S" uniqKey="Sambanthamoorthy S" first="S" last="Sambanthamoorthy">S. Sambanthamoorthy</name></author><author><name sortKey="Dakshinamoorthy, G" sort="Dakshinamoorthy, G" uniqKey="Dakshinamoorthy G" first="G" last="Dakshinamoorthy">G. Dakshinamoorthy</name></author><author><name sortKey="Munirathinam, G" sort="Munirathinam, G" uniqKey="Munirathinam G" first="G" last="Munirathinam">G. Munirathinam</name></author><author><name sortKey="Ramaswamy, K" sort="Ramaswamy, K" uniqKey="Ramaswamy K" first="K" last="Ramaswamy">K. Ramaswamy</name></author></analytic><series><title level="j">Vaccine</title><idno type="eISSN">1873-2518</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Helminth (immunology)</term><term>Antigens, Helminth (genetics)</term><term>Antigens, Helminth (immunology)</term><term>Biolistics (methods)</term><term>COS Cells</term><term>Cercopithecus aethiops</term><term>DNA, Helminth (immunology)</term><term>Elephantiasis, Filarial (prevention & control)</term><term>Immunoglobulin G (immunology)</term><term>Injections, Intradermal</term><term>Interferon-gamma (immunology)</term><term>Interleukin-12 (immunology)</term><term>Interleukin-4 (immunology)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (immunology)</term><term>Tumor Necrosis Factor-alpha (immunology)</term><term>Vaccines (immunology)</term><term>Vaccines, DNA (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN des helminthes (immunologie)</term><term>Animaux</term><term>Anticorps antihelminthe (immunologie)</term><term>Antigènes d'helminthe (génétique)</term><term>Antigènes d'helminthe (immunologie)</term><term>Biolistique ()</term><term>Cellules COS</term><term>Cercopithecus aethiops</term><term>Facteur de nécrose tumorale alpha (immunologie)</term><term>Filariose lymphatique ()</term><term>Immunoglobuline G (immunologie)</term><term>Injections intradermiques</term><term>Interféron gamma (immunologie)</term><term>Interleukine-12 (immunologie)</term><term>Interleukine-4 (immunologie)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (immunologie)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Vaccins (immunologie)</term><term>Vaccins à ADN (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Helminth</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Helminth</term><term>Antigens, Helminth</term><term>DNA, Helminth</term><term>Immunoglobulin G</term><term>Interferon-gamma</term><term>Interleukin-12</term><term>Interleukin-4</term><term>Recombinant Proteins</term><term>Tumor Necrosis Factor-alpha</term><term>Vaccines</term><term>Vaccines, DNA</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Antigènes d'helminthe</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>ADN des helminthes</term><term>Anticorps antihelminthe</term><term>Antigènes d'helminthe</term><term>Facteur de nécrose tumorale alpha</term><term>Immunoglobuline G</term><term>Interféron gamma</term><term>Interleukine-12</term><term>Interleukine-4</term><term>Protéines recombinantes</term><term>Vaccins</term><term>Vaccins à ADN</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biolistics</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>COS Cells</term><term>Cercopithecus aethiops</term><term>Injections, Intradermal</term><term>Mice</term><term>Mice, Inbred BALB C</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Biolistique</term><term>Cellules COS</term><term>Cercopithecus aethiops</term><term>Filariose lymphatique</term><term>Injections intradermiques</term><term>Souris</term><term>Souris de lignée BALB C</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Biolistic vaccination using gene gun is developed as a safer tool for delivery of DNA vaccines, a technique that combines high vaccine efficiency with lower antigen dosage and lower cost per vaccine dose. In this study, we compared the protective responses in mice after delivering the Brugia malayi abundant larval transcript-2 (BmALT-2) DNA vaccine using the conventional intradermal approach or with the needleless gene gun delivery approach. BmALT-2 is a leading vaccine candidate against B. malayi, a lymphatic filarial parasite of human. After optimizing the DNA dose and gene gun parameters for delivery into mouse skin, groups of mice were biolistically vaccinated with 5 μg of BmALT-2pVAX. Groups of mice vaccinated intradermally with 5 μg or 100 μg of BmALT-2pVAX was used for comparison of vaccine efficacy. Results demonstrated that gene gun vaccination with 5 μg of BmALT-2pVAX conferred significant protection against challenge infection that was comparable to the degree of protection conferred by intradermal vaccination with 100 μg of BmALT-2pVAX. This observation was further supported by an in vitro antibody dependent cellular cytotoxicity (ADCC) assay. Analysis of the immune response showed that the gene gun vaccination predominantly induced an IgG1 antibody response and significantly high Th2 cytokine response (IL-4) from spleen cells compared to intradermal BmALT-2 DNA delivery that induced predominantly an IgG2a and Th1 cytokine response (IFN-γ, IL-12 and TNF-α). These findings show that host protective responses could be achieved with 20 fold decrease in DNA dose using a gene gun and could prove to be an efficient delivery method in BmALT-2 DNA vaccination against lymphatic filariasis.</div></front></TEI></pubmed></double></record>Pour manipuler ce document sous Unix (Dilib)
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