Identification of antigenic Brugia adult worm proteins by peptide mass fingerprinting.
Identifieur interne : 003474 ( Ncbi/Checkpoint ); précédent : 003473; suivant : 003475Identification of antigenic Brugia adult worm proteins by peptide mass fingerprinting.
Auteurs : Tiffany Weinkopff [États-Unis] ; James A. Atwood ; George A. Punkosdy ; Delynn Moss ; D Brent Weatherly ; Ron Orlando ; Patrick LammieSource :
- The Journal of parasitology [ 1937-2345 ] ; 2009.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antihelminthe (sang), Antigènes d'helminthe (), Antigènes d'helminthe (analyse), Antigènes d'helminthe (immunologie), Brugia pahangi (), Brugia pahangi (immunologie), Données de séquences moléculaires, Filariose lymphatique (immunologie), Filariose lymphatique (parasitologie), Filariose lymphatique (sang), Gerbillinae, Humains, Immunotransfert, Protéines d'helminthes (), Protéines d'helminthes (analyse), Protéines d'helminthes (immunologie), Protéomique, Spectrométrie de masse MALDI, Séquence d'acides aminés, Traitement d'image par ordinateur, Électrophorèse bidimensionnelle sur gel, Électrophorèse sur gel de polyacrylamide.
- MESH :
- analyse : Antigènes d'helminthe, Protéines d'helminthes.
- immunologie : Antigènes d'helminthe, Brugia pahangi, Filariose lymphatique, Protéines d'helminthes.
- parasitologie : Filariose lymphatique.
- sang : Anticorps antihelminthe, Filariose lymphatique.
- Animaux, Antigènes d'helminthe, Brugia pahangi, Données de séquences moléculaires, Gerbillinae, Humains, Immunotransfert, Protéines d'helminthes, Protéomique, Spectrométrie de masse MALDI, Séquence d'acides aminés, Traitement d'image par ordinateur, Électrophorèse bidimensionnelle sur gel, Électrophorèse sur gel de polyacrylamide.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Helminth (blood), Antigens, Helminth (analysis), Antigens, Helminth (chemistry), Antigens, Helminth (immunology), Brugia pahangi (chemistry), Brugia pahangi (immunology), Electrophoresis, Gel, Two-Dimensional, Electrophoresis, Polyacrylamide Gel, Elephantiasis, Filarial (blood), Elephantiasis, Filarial (immunology), Elephantiasis, Filarial (parasitology), Gerbillinae, Helminth Proteins (analysis), Helminth Proteins (chemistry), Helminth Proteins (immunology), Humans, Image Processing, Computer-Assisted, Immunoblotting, Molecular Sequence Data, Proteomics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.
- MESH :
- chemical , analysis : Antigens, Helminth, Helminth Proteins.
- chemical , blood : Antibodies, Helminth.
- chemical , chemistry : Antigens, Helminth, Helminth Proteins.
- chemical , immunology : Antigens, Helminth, Helminth Proteins.
- blood : Elephantiasis, Filarial.
- chemistry : Brugia pahangi.
- immunology : Brugia pahangi, Elephantiasis, Filarial.
- parasitology : Elephantiasis, Filarial.
- Amino Acid Sequence, Animals, Electrophoresis, Gel, Two-Dimensional, Electrophoresis, Polyacrylamide Gel, Gerbillinae, Humans, Image Processing, Computer-Assisted, Immunoblotting, Molecular Sequence Data, Proteomics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.
Abstract
With the recent completion of the Brugia malayi genome, proteomics offers a new resource for a deeper understanding of the biology of filarial parasites. We employed 2-dimensional (2D) gel electrophoresis followed by peptide mass fingerprinting on a matrix-assisted laser desorption/ionization time-of-flight (MALDI-ToF) mass spectrometer to identify Brugia adult worm proteins and then determined which proteins were recognized by the host humoral immune response. We identified 18 unique proteins, several of which were determined to be antigenic by immunoblot. The proteins identified here may contribute to future studies to analyze the transmission and pathogenesis of lymphatic filariasis.
DOI: 10.1645/GE-2083.1
PubMed: 19537848
Affiliations:
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(analyse)</term><term>Antigènes d'helminthe (immunologie)</term><term>Brugia pahangi ()</term><term>Brugia pahangi (immunologie)</term><term>Données de séquences moléculaires</term><term>Filariose lymphatique (immunologie)</term><term>Filariose lymphatique (parasitologie)</term><term>Filariose lymphatique (sang)</term><term>Gerbillinae</term><term>Humains</term><term>Immunotransfert</term><term>Protéines d'helminthes ()</term><term>Protéines d'helminthes (analyse)</term><term>Protéines d'helminthes (immunologie)</term><term>Protéomique</term><term>Spectrométrie de masse MALDI</term><term>Séquence d'acides aminés</term><term>Traitement d'image par ordinateur</term><term>Électrophorèse bidimensionnelle sur gel</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antigens, Helminth</term><term>Helminth Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" 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de séquences moléculaires</term><term>Gerbillinae</term><term>Humains</term><term>Immunotransfert</term><term>Protéines d'helminthes</term><term>Protéomique</term><term>Spectrométrie de masse MALDI</term><term>Séquence d'acides aminés</term><term>Traitement d'image par ordinateur</term><term>Électrophorèse bidimensionnelle sur gel</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">With the recent completion of the Brugia malayi genome, proteomics offers a new resource for a deeper understanding of the biology of filarial parasites. We employed 2-dimensional (2D) gel electrophoresis followed by peptide mass fingerprinting on a matrix-assisted laser desorption/ionization time-of-flight (MALDI-ToF) mass spectrometer to identify Brugia adult worm proteins and then determined which proteins were recognized by the host humoral immune response. We identified 18 unique proteins, several of which were determined to be antigenic by immunoblot. The proteins identified here may contribute to future studies to analyze the transmission and pathogenesis of lymphatic filariasis.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Atwood, James A" sort="Atwood, James A" uniqKey="Atwood J" first="James A" last="Atwood">James A. Atwood</name><name sortKey="Lammie, Patrick" sort="Lammie, Patrick" uniqKey="Lammie P" first="Patrick" last="Lammie">Patrick Lammie</name><name sortKey="Moss, Delynn" sort="Moss, Delynn" uniqKey="Moss D" first="Delynn" last="Moss">Delynn Moss</name><name sortKey="Orlando, Ron" sort="Orlando, Ron" uniqKey="Orlando R" first="Ron" last="Orlando">Ron Orlando</name><name sortKey="Punkosdy, George A" sort="Punkosdy, George A" uniqKey="Punkosdy G" first="George A" last="Punkosdy">George A. Punkosdy</name><name sortKey="Weatherly, D Brent" sort="Weatherly, D Brent" uniqKey="Weatherly D" first="D Brent" last="Weatherly">D Brent Weatherly</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Weinkopff, Tiffany" sort="Weinkopff, Tiffany" uniqKey="Weinkopff T" first="Tiffany" last="Weinkopff">Tiffany Weinkopff</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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