Identification of antigenic Brugia adult worm proteins by peptide mass fingerprinting.
Identifieur interne : 002E27 ( PubMed/Corpus ); précédent : 002E26; suivant : 002E28Identification of antigenic Brugia adult worm proteins by peptide mass fingerprinting.
Auteurs : Tiffany Weinkopff ; James A. Atwood ; George A. Punkosdy ; Delynn Moss ; D Brent Weatherly ; Ron Orlando ; Patrick LammieSource :
- The Journal of parasitology [ 1937-2345 ] ; 2009.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Helminth (blood), Antigens, Helminth (analysis), Antigens, Helminth (chemistry), Antigens, Helminth (immunology), Brugia pahangi (chemistry), Brugia pahangi (immunology), Electrophoresis, Gel, Two-Dimensional, Electrophoresis, Polyacrylamide Gel, Elephantiasis, Filarial (blood), Elephantiasis, Filarial (immunology), Elephantiasis, Filarial (parasitology), Gerbillinae, Helminth Proteins (analysis), Helminth Proteins (chemistry), Helminth Proteins (immunology), Humans, Image Processing, Computer-Assisted, Immunoblotting, Molecular Sequence Data, Proteomics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.
- MESH :
- chemical , analysis : Antigens, Helminth, Helminth Proteins.
- chemical , blood : Antibodies, Helminth.
- chemical , chemistry : Antigens, Helminth, Helminth Proteins.
- chemical , immunology : Antigens, Helminth, Helminth Proteins.
- blood : Elephantiasis, Filarial.
- chemistry : Brugia pahangi.
- immunology : Brugia pahangi, Elephantiasis, Filarial.
- parasitology : Elephantiasis, Filarial.
- Amino Acid Sequence, Animals, Electrophoresis, Gel, Two-Dimensional, Electrophoresis, Polyacrylamide Gel, Gerbillinae, Humans, Image Processing, Computer-Assisted, Immunoblotting, Molecular Sequence Data, Proteomics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.
Abstract
With the recent completion of the Brugia malayi genome, proteomics offers a new resource for a deeper understanding of the biology of filarial parasites. We employed 2-dimensional (2D) gel electrophoresis followed by peptide mass fingerprinting on a matrix-assisted laser desorption/ionization time-of-flight (MALDI-ToF) mass spectrometer to identify Brugia adult worm proteins and then determined which proteins were recognized by the host humoral immune response. We identified 18 unique proteins, several of which were determined to be antigenic by immunoblot. The proteins identified here may contribute to future studies to analyze the transmission and pathogenesis of lymphatic filariasis.
DOI: 10.1645/GE-2083.1
PubMed: 19537848
Links to Exploration step
pubmed:19537848Le document en format XML
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<affiliation><nlm:affiliation>Department of Cell Biology, 724 Biological Sciences Building, University of Georgia, Athens, Georgia 30602, USA.</nlm:affiliation>
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<author><name sortKey="Atwood, James A" sort="Atwood, James A" uniqKey="Atwood J" first="James A" last="Atwood">James A. Atwood</name>
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<author><name sortKey="Moss, Delynn" sort="Moss, Delynn" uniqKey="Moss D" first="Delynn" last="Moss">Delynn Moss</name>
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<term>Antigens, Helminth (chemistry)</term>
<term>Antigens, Helminth (immunology)</term>
<term>Brugia pahangi (chemistry)</term>
<term>Brugia pahangi (immunology)</term>
<term>Electrophoresis, Gel, Two-Dimensional</term>
<term>Electrophoresis, Polyacrylamide Gel</term>
<term>Elephantiasis, Filarial (blood)</term>
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<term>Image Processing, Computer-Assisted</term>
<term>Immunoblotting</term>
<term>Molecular Sequence Data</term>
<term>Proteomics</term>
<term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term>
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<term>Electrophoresis, Polyacrylamide Gel</term>
<term>Gerbillinae</term>
<term>Humans</term>
<term>Image Processing, Computer-Assisted</term>
<term>Immunoblotting</term>
<term>Molecular Sequence Data</term>
<term>Proteomics</term>
<term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term>
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<front><div type="abstract" xml:lang="en">With the recent completion of the Brugia malayi genome, proteomics offers a new resource for a deeper understanding of the biology of filarial parasites. We employed 2-dimensional (2D) gel electrophoresis followed by peptide mass fingerprinting on a matrix-assisted laser desorption/ionization time-of-flight (MALDI-ToF) mass spectrometer to identify Brugia adult worm proteins and then determined which proteins were recognized by the host humoral immune response. We identified 18 unique proteins, several of which were determined to be antigenic by immunoblot. The proteins identified here may contribute to future studies to analyze the transmission and pathogenesis of lymphatic filariasis.</div>
</front>
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