A technique for microfilarial detection in preserved blood using nuclepore filters.
Identifieur interne : 00E681 ( Main/Merge ); précédent : 00E680; suivant : 00E682A technique for microfilarial detection in preserved blood using nuclepore filters.
Auteurs : J W Dickerson [États-Unis] ; M L Eberhard ; P J LammieSource :
- The Journal of parasitology [ 0022-3395 ] ; 1990.
Descripteurs français
- KwdFr :
- MESH :
- isolement et purification : Microfilaria, Wuchereria bancrofti.
- parasitologie : Filariose lymphatique.
- sang : Filariose lymphatique.
- Acide citrique, Acide édétique, Alcools gras, Animaux, Citrates, Conservation de sang, Filtration, Formaldéhyde, Humains, Tensioactifs.
English descriptors
- KwdEn :
- Animals, Blood Preservation, Citrates, Citric Acid, Edetic Acid, Elephantiasis, Filarial (blood), Elephantiasis, Filarial (parasitology), Fatty Alcohols, Filtration, Formaldehyde, Humans, Microfilariae (isolation & purification), Surface-Active Agents, Wuchereria bancrofti (isolation & purification).
- MESH :
- chemical : Citrates, Citric Acid, Edetic Acid, Fatty Alcohols, Formaldehyde, Surface-Active Agents.
- blood : Elephantiasis, Filarial.
- isolation & purification : Microfilariae, Wuchereria bancrofti.
- parasitology : Elephantiasis, Filarial.
- Animals, Blood Preservation, Filtration, Humans.
Abstract
Nuclepore filtration is now the most widely used method of detecting microfilaremia, particularly if microfilariae are few in number. However, this system requires the blood sample to be processed promptly after collection. Using blood from Wuchereria bancrofti-infected patients, 3 solutions were tested to determine whether blood could be held for delayed processing. Of these, we identified 1, 2% formalin/10% Teepol, in which microfilaremic blood can be held for at least 9 mo without deterioration of microfilarial structure or decrease in microfilarial numbers. In addition, this mixture passed easily through a 5-microns filter at all times tested. Examination of more than 300 blood samples held in 2% formalin/10% Teepol showed that this solution can utilize the convenience and sensitivity of membrane filtration while eliminating the need to perform testing immediately after the blood is collected.
PubMed: 2123924
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pubmed:2123924Le document en format XML
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<series><title level="j">The Journal of parasitology</title>
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<term>Blood Preservation</term>
<term>Citrates</term>
<term>Citric Acid</term>
<term>Edetic Acid</term>
<term>Elephantiasis, Filarial (blood)</term>
<term>Elephantiasis, Filarial (parasitology)</term>
<term>Fatty Alcohols</term>
<term>Filtration</term>
<term>Formaldehyde</term>
<term>Humans</term>
<term>Microfilariae (isolation & purification)</term>
<term>Surface-Active Agents</term>
<term>Wuchereria bancrofti (isolation & purification)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Acide citrique</term>
<term>Acide édétique</term>
<term>Alcools gras</term>
<term>Animaux</term>
<term>Citrates</term>
<term>Conservation de sang</term>
<term>Filariose lymphatique (parasitologie)</term>
<term>Filariose lymphatique (sang)</term>
<term>Filtration</term>
<term>Formaldéhyde</term>
<term>Humains</term>
<term>Microfilaria (isolement et purification)</term>
<term>Tensioactifs</term>
<term>Wuchereria bancrofti (isolement et purification)</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Citrates</term>
<term>Citric Acid</term>
<term>Edetic Acid</term>
<term>Fatty Alcohols</term>
<term>Formaldehyde</term>
<term>Surface-Active Agents</term>
</keywords>
<keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Elephantiasis, Filarial</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Microfilariae</term>
<term>Wuchereria bancrofti</term>
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<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Microfilaria</term>
<term>Wuchereria bancrofti</term>
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<keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Filariose lymphatique</term>
</keywords>
<keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Elephantiasis, Filarial</term>
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<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Filariose lymphatique</term>
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<term>Blood Preservation</term>
<term>Filtration</term>
<term>Humans</term>
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<term>Acide édétique</term>
<term>Alcools gras</term>
<term>Animaux</term>
<term>Citrates</term>
<term>Conservation de sang</term>
<term>Filtration</term>
<term>Formaldéhyde</term>
<term>Humains</term>
<term>Tensioactifs</term>
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<front><div type="abstract" xml:lang="en">Nuclepore filtration is now the most widely used method of detecting microfilaremia, particularly if microfilariae are few in number. However, this system requires the blood sample to be processed promptly after collection. Using blood from Wuchereria bancrofti-infected patients, 3 solutions were tested to determine whether blood could be held for delayed processing. Of these, we identified 1, 2% formalin/10% Teepol, in which microfilaremic blood can be held for at least 9 mo without deterioration of microfilarial structure or decrease in microfilarial numbers. In addition, this mixture passed easily through a 5-microns filter at all times tested. Examination of more than 300 blood samples held in 2% formalin/10% Teepol showed that this solution can utilize the convenience and sensitivity of membrane filtration while eliminating the need to perform testing immediately after the blood is collected.</div>
</front>
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