The alarmin HMGB‐1 influences healing outcomes in fetal skin wounds
Identifieur interne : 003A44 ( Main/Merge ); précédent : 003A43; suivant : 003A45The alarmin HMGB‐1 influences healing outcomes in fetal skin wounds
Auteurs : Adrienne D. Dardenne ; Brian C. Wulff ; Traci A. Wilgus [États-Unis]Source :
- Wound Repair and Regeneration [ 1067-1927 ] ; 2013-03.
Abstract
In mice, cutaneous wounds generated early in development (embryonic day 15, E15) heal scarlessly, while wounds generated late in gestation (embryonic day 18, E18) heal with scar formation. Even though both types of wounds are generated in the same sterile uterine environment, scarless fetal wounds heal without inflammation, but a strong inflammatory response is observed in scar‐forming fetal wounds. We hypothesized that altered release of alarmins, endogenous molecules that trigger inflammation in response to damage, may be responsible for the age‐related changes in inflammation and healing outcomes in fetal skin. The purpose of this study was to determine whether the alarmin high‐mobility group box‐1 (HMGB‐1) is involved in fetal wound repair. Immunohistochemical analysis showed that in unwounded skin, E18 keratinocytes expressed higher levels of HMGB‐1 compared with E15 keratinocytes. After injury, HMGB‐1 was released to a greater extent from keratinocytes at the margin of scar‐forming E18 wounds, compared with scarless E15 wounds. Furthermore, instead of healing scarlessly, E15 wounds healed with scars when treated with HMGB‐1. HMGB‐1‐injected wounds also had more fibroblasts, blood vessels, and macrophages compared with control wounds. Together, these data suggest that extracellular HMGB‐1 levels influence the quality of healing in cutaneous wounds.
Url:
- https://api.istex.fr/document/A96DCA10748A4825D1C6355B871575F1BF94F146/fulltext/pdf
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3594575
DOI: 10.1111/wrr.12028
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 004F70
- to stream Istex, to step Curation: 004F70
- to stream Istex, to step Checkpoint: 000063
- to stream Pmc, to step Corpus: 003963
- to stream Pmc, to step Curation: 003962
- to stream Pmc, to step Checkpoint: 001F19
- to stream Ncbi, to step Merge: 005647
- to stream Ncbi, to step Curation: 005647
- to stream Ncbi, to step Checkpoint: 005647
Links to Exploration step
ISTEX:A96DCA10748A4825D1C6355B871575F1BF94F146Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">The alarmin HMGB‐1 influences healing outcomes in fetal skin wounds</title>
<author><name sortKey="Dardenne, Adrienne D" sort="Dardenne, Adrienne D" uniqKey="Dardenne A" first="Adrienne D." last="Dardenne">Adrienne D. Dardenne</name>
</author>
<author><name sortKey="Wulff, Brian C" sort="Wulff, Brian C" uniqKey="Wulff B" first="Brian C." last="Wulff">Brian C. Wulff</name>
</author>
<author><name sortKey="Wilgus, Traci A" sort="Wilgus, Traci A" uniqKey="Wilgus T" first="Traci A." last="Wilgus">Traci A. Wilgus</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:A96DCA10748A4825D1C6355B871575F1BF94F146</idno>
<date when="2013" year="2013">2013</date>
<idno type="doi">10.1111/wrr.12028</idno>
<idno type="url">https://api.istex.fr/document/A96DCA10748A4825D1C6355B871575F1BF94F146/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">004F70</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">004F70</idno>
<idno type="wicri:Area/Istex/Curation">004F70</idno>
<idno type="wicri:Area/Istex/Checkpoint">000063</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000063</idno>
<idno type="wicri:doubleKey">1067-1927:2013:Dardenne A:the:alarmin:hmgb</idno>
<idno type="wicri:source">PMC</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3594575</idno>
<idno type="RBID">PMC:3594575</idno>
<idno type="wicri:Area/Pmc/Corpus">003963</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">003963</idno>
<idno type="wicri:Area/Pmc/Curation">003962</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">003962</idno>
<idno type="wicri:Area/Pmc/Checkpoint">001F19</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001F19</idno>
<idno type="wicri:Area/Ncbi/Merge">005647</idno>
<idno type="wicri:Area/Ncbi/Curation">005647</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">005647</idno>
<idno type="wicri:doubleKey">1067-1927:2013:Dardenne A:the:alarmin:hmgb</idno>
<idno type="wicri:Area/Main/Merge">003A44</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main">The alarmin <hi rend="fc">HMGB</hi>
‐1 influences healing outcomes in fetal skin wounds</title>
<author><name sortKey="Dardenne, Adrienne D" sort="Dardenne, Adrienne D" uniqKey="Dardenne A" first="Adrienne D." last="Dardenne">Adrienne D. Dardenne</name>
<affiliation><wicri:noCountry code="subField">Columbus</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Wulff, Brian C" sort="Wulff, Brian C" uniqKey="Wulff B" first="Brian C." last="Wulff">Brian C. Wulff</name>
<affiliation><wicri:noCountry code="subField">Columbus</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Wilgus, Traci A" sort="Wilgus, Traci A" uniqKey="Wilgus T" first="Traci A." last="Wilgus">Traci A. Wilgus</name>
<affiliation></affiliation>
<affiliation></affiliation>
<affiliation wicri:level="1"><country wicri:rule="url">États-Unis</country>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j" type="main">Wound Repair and Regeneration</title>
<title level="j" type="alt">WOUND REPAIR AND REGENERATION</title>
<idno type="ISSN">1067-1927</idno>
<idno type="eISSN">1524-475X</idno>
<imprint><biblScope unit="vol">21</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="282">282</biblScope>
<biblScope unit="page" to="291">291</biblScope>
<biblScope unit="page-count">10</biblScope>
<date type="published" when="2013-03">2013-03</date>
</imprint>
<idno type="ISSN">1067-1927</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">1067-1927</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract">In mice, cutaneous wounds generated early in development (embryonic day 15, E15) heal scarlessly, while wounds generated late in gestation (embryonic day 18, E18) heal with scar formation. Even though both types of wounds are generated in the same sterile uterine environment, scarless fetal wounds heal without inflammation, but a strong inflammatory response is observed in scar‐forming fetal wounds. We hypothesized that altered release of alarmins, endogenous molecules that trigger inflammation in response to damage, may be responsible for the age‐related changes in inflammation and healing outcomes in fetal skin. The purpose of this study was to determine whether the alarmin high‐mobility group box‐1 (HMGB‐1) is involved in fetal wound repair. Immunohistochemical analysis showed that in unwounded skin, E18 keratinocytes expressed higher levels of HMGB‐1 compared with E15 keratinocytes. After injury, HMGB‐1 was released to a greater extent from keratinocytes at the margin of scar‐forming E18 wounds, compared with scarless E15 wounds. Furthermore, instead of healing scarlessly, E15 wounds healed with scars when treated with HMGB‐1. HMGB‐1‐injected wounds also had more fibroblasts, blood vessels, and macrophages compared with control wounds. Together, these data suggest that extracellular HMGB‐1 levels influence the quality of healing in cutaneous wounds.</div>
</front>
</TEI>
<double doi="10.1111/wrr.12028"><ISTEX><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">The alarmin HMGB‐1 influences healing outcomes in fetal skin wounds</title>
<author><name sortKey="Dardenne, Adrienne D" sort="Dardenne, Adrienne D" uniqKey="Dardenne A" first="Adrienne D." last="Dardenne">Adrienne D. Dardenne</name>
</author>
<author><name sortKey="Wulff, Brian C" sort="Wulff, Brian C" uniqKey="Wulff B" first="Brian C." last="Wulff">Brian C. Wulff</name>
</author>
<author><name sortKey="Wilgus, Traci A" sort="Wilgus, Traci A" uniqKey="Wilgus T" first="Traci A." last="Wilgus">Traci A. Wilgus</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:A96DCA10748A4825D1C6355B871575F1BF94F146</idno>
<date when="2013" year="2013">2013</date>
<idno type="doi">10.1111/wrr.12028</idno>
<idno type="url">https://api.istex.fr/document/A96DCA10748A4825D1C6355B871575F1BF94F146/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">004F70</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">004F70</idno>
<idno type="wicri:Area/Istex/Curation">004F70</idno>
<idno type="wicri:Area/Istex/Checkpoint">000063</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000063</idno>
<idno type="wicri:doubleKey">1067-1927:2013:Dardenne A:the:alarmin:hmgb</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main">The alarmin <hi rend="fc">HMGB</hi>
‐1 influences healing outcomes in fetal skin wounds</title>
<author><name sortKey="Dardenne, Adrienne D" sort="Dardenne, Adrienne D" uniqKey="Dardenne A" first="Adrienne D." last="Dardenne">Adrienne D. Dardenne</name>
<affiliation><wicri:noCountry code="subField">Columbus</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Wulff, Brian C" sort="Wulff, Brian C" uniqKey="Wulff B" first="Brian C." last="Wulff">Brian C. Wulff</name>
<affiliation><wicri:noCountry code="subField">Columbus</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Wilgus, Traci A" sort="Wilgus, Traci A" uniqKey="Wilgus T" first="Traci A." last="Wilgus">Traci A. Wilgus</name>
<affiliation></affiliation>
<affiliation></affiliation>
<affiliation wicri:level="1"><country wicri:rule="url">États-Unis</country>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j" type="main">Wound Repair and Regeneration</title>
<title level="j" type="alt">WOUND REPAIR AND REGENERATION</title>
<idno type="ISSN">1067-1927</idno>
<idno type="eISSN">1524-475X</idno>
<imprint><biblScope unit="vol">21</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="282">282</biblScope>
<biblScope unit="page" to="291">291</biblScope>
<biblScope unit="page-count">10</biblScope>
<date type="published" when="2013-03">2013-03</date>
</imprint>
<idno type="ISSN">1067-1927</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">1067-1927</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract">In mice, cutaneous wounds generated early in development (embryonic day 15, E15) heal scarlessly, while wounds generated late in gestation (embryonic day 18, E18) heal with scar formation. Even though both types of wounds are generated in the same sterile uterine environment, scarless fetal wounds heal without inflammation, but a strong inflammatory response is observed in scar‐forming fetal wounds. We hypothesized that altered release of alarmins, endogenous molecules that trigger inflammation in response to damage, may be responsible for the age‐related changes in inflammation and healing outcomes in fetal skin. The purpose of this study was to determine whether the alarmin high‐mobility group box‐1 (HMGB‐1) is involved in fetal wound repair. Immunohistochemical analysis showed that in unwounded skin, E18 keratinocytes expressed higher levels of HMGB‐1 compared with E15 keratinocytes. After injury, HMGB‐1 was released to a greater extent from keratinocytes at the margin of scar‐forming E18 wounds, compared with scarless E15 wounds. Furthermore, instead of healing scarlessly, E15 wounds healed with scars when treated with HMGB‐1. HMGB‐1‐injected wounds also had more fibroblasts, blood vessels, and macrophages compared with control wounds. Together, these data suggest that extracellular HMGB‐1 levels influence the quality of healing in cutaneous wounds.</div>
</front>
</TEI>
</ISTEX>
<PMC><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The alarmin HMGB-1 influences healing outcomes in fetal skin wounds</title>
<author><name sortKey="Dardenne, Adrienne D" sort="Dardenne, Adrienne D" uniqKey="Dardenne A" first="Adrienne D." last="Dardenne">Adrienne D. Dardenne</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Department of Veterinary Preventive Medicine/University Laboratory Animal Resources, Columbus, OH</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Ohio</region>
</placeName>
<wicri:cityArea>Department of Veterinary Preventive Medicine/University Laboratory Animal Resources, Columbus</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Wulff, Brian C" sort="Wulff, Brian C" uniqKey="Wulff B" first="Brian C." last="Wulff">Brian C. Wulff</name>
<affiliation wicri:level="2"><nlm:aff id="A2">Department of Pathology, The Ohio State University, Columbus, OH</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Ohio</region>
</placeName>
<wicri:cityArea>Department of Pathology, The Ohio State University, Columbus</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Wilgus, Traci A" sort="Wilgus, Traci A" uniqKey="Wilgus T" first="Traci A." last="Wilgus">Traci A. Wilgus</name>
<affiliation wicri:level="2"><nlm:aff id="A2">Department of Pathology, The Ohio State University, Columbus, OH</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Ohio</region>
</placeName>
<wicri:cityArea>Department of Pathology, The Ohio State University, Columbus</wicri:cityArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">23438257</idno>
<idno type="pmc">3594575</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3594575</idno>
<idno type="RBID">PMC:3594575</idno>
<idno type="doi">10.1111/wrr.12028</idno>
<date when="2013">2013</date>
<idno type="wicri:Area/Pmc/Corpus">003963</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">003963</idno>
<idno type="wicri:Area/Pmc/Curation">003962</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">003962</idno>
<idno type="wicri:Area/Pmc/Checkpoint">001F19</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001F19</idno>
<idno type="wicri:Area/Ncbi/Merge">005647</idno>
<idno type="wicri:Area/Ncbi/Curation">005647</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">005647</idno>
<idno type="wicri:doubleKey">1067-1927:2013:Dardenne A:the:alarmin:hmgb</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The alarmin HMGB-1 influences healing outcomes in fetal skin wounds</title>
<author><name sortKey="Dardenne, Adrienne D" sort="Dardenne, Adrienne D" uniqKey="Dardenne A" first="Adrienne D." last="Dardenne">Adrienne D. Dardenne</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Department of Veterinary Preventive Medicine/University Laboratory Animal Resources, Columbus, OH</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Ohio</region>
</placeName>
<wicri:cityArea>Department of Veterinary Preventive Medicine/University Laboratory Animal Resources, Columbus</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Wulff, Brian C" sort="Wulff, Brian C" uniqKey="Wulff B" first="Brian C." last="Wulff">Brian C. Wulff</name>
<affiliation wicri:level="2"><nlm:aff id="A2">Department of Pathology, The Ohio State University, Columbus, OH</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Ohio</region>
</placeName>
<wicri:cityArea>Department of Pathology, The Ohio State University, Columbus</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Wilgus, Traci A" sort="Wilgus, Traci A" uniqKey="Wilgus T" first="Traci A." last="Wilgus">Traci A. Wilgus</name>
<affiliation wicri:level="2"><nlm:aff id="A2">Department of Pathology, The Ohio State University, Columbus, OH</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Ohio</region>
</placeName>
<wicri:cityArea>Department of Pathology, The Ohio State University, Columbus</wicri:cityArea>
</affiliation>
</author>
</analytic>
<series><title level="j">Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society</title>
<idno type="ISSN">1067-1927</idno>
<idno type="eISSN">1524-475X</idno>
<imprint><date when="2013">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p id="P1">In mice, cutaneous wounds generated early in development (embryonic day 15, E15) heal scarlessly, while wounds generated late in gestation (embryonic day 18, E18) heal with scar formation. Even though both types of wounds are generated in the same sterile uterine environment, scarless fetal wounds heal without inflammation but a strong inflammatory response is observed in scar-forming fetal wounds. We hypothesized that altered release of alarmins, endogenous molecules that trigger inflammation in response to damage, may be responsible for the age-related changes in inflammation and healing outcomes in fetal skin. The purpose of this study was to determine whether the alarmin high-mobility group box -1 (HMGB-1) is involved in fetal wound repair. Immunohistochemical analysis showed that in unwounded skin, E18 keratinocytes expressed higher levels of HMGB-1 compared to E15 keratinocytes. After injury, HMGB-1 was released to a greater extent from keratinocytes at the margin of scar-forming E18 wounds compared to scarless E15 wounds. Furthermore, instead of healing scarlessly, E15 wounds healed with scars when treated with HMGB-1. HMGB-1-injected wounds also had more fibroblasts, blood vessels, and macrophages compared to control wounds. Together, these data suggest that extracellular HMGB-1 levels influence the quality of healing in cutaneous wounds.</p>
</div>
</front>
</TEI>
</PMC>
</double>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003A44 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 003A44 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= LymphedemaV1 |flux= Main |étape= Merge |type= RBID |clé= ISTEX:A96DCA10748A4825D1C6355B871575F1BF94F146 |texte= The alarmin HMGB‐1 influences healing outcomes in fetal skin wounds }}
![]() | This area was generated with Dilib version V0.6.31. | ![]() |