Rapid diagnosis of Brugia malayi and Wuchereria bancrofti filariasis by an acridine orange/microhematocrit tube technique.
Identifieur interne : 00D980 ( Main/Exploration ); précédent : 00D979; suivant : 00D981Rapid diagnosis of Brugia malayi and Wuchereria bancrofti filariasis by an acridine orange/microhematocrit tube technique.
Auteurs : G W Long [États-Unis] ; L S Rickman ; J H CrossSource :
- The Journal of parasitology [ 0022-3395 ] ; 1990.
Descripteurs français
- KwdFr :
- Animaux, Brugia (isolement et purification), Centrifugation, Coloration et marquage, Filariose lymphatique (diagnostic), Filarioses (diagnostic), Humains, Hématocrite, Microscopie de fluorescence, Orange acridine, Wuchereria (isolement et purification), Wuchereria bancrofti (isolement et purification).
- MESH :
- diagnostic : Filariose lymphatique, Filarioses.
- isolement et purification : Brugia, Wuchereria, Wuchereria bancrofti.
- Animaux, Centrifugation, Coloration et marquage, Humains, Hématocrite, Microscopie de fluorescence, Orange acridine.
English descriptors
- KwdEn :
- MESH :
- chemical : Acridine Orange.
- diagnosis : Elephantiasis, Filarial, Filariasis.
- isolation & purification : Brugia, Wuchereria, Wuchereria bancrofti.
- Animals, Centrifugation, Hematocrit, Humans, Microscopy, Fluorescence, Staining and Labeling.
Abstract
A microhematocrit tube technique for diagnosis of human filariasis has been previously described. A system incorporating heparin, EDTA, and acridine orange into a microhematocrit tube (Quantitative Blood Count, QBC) has been commercially developed for the quantitation of blood counts and has been used for the diagnosis of malaria. We evaluated this test for its usefulness in the diagnosis of filariasis. Upon centrifugation, the parasites were concentrated in the area of the buffy coat and could be observed through the wall of the tube. The parasites were concentrated further by a plastic float that expands the buffy coat and confines the parasites to the periphery of the tube. Acridine orange stains the DNA of the parasite, and morphologic characteristics can be examined by fluorescence microscopy. The terminal and subterminal nuclei and long cephalic space of Brugia malayi, as well as the short cephalic space and caudal nuclei of Wuchereria bancrofti, were easily recognized and differentiated from each other. Microfilariae were detected in samples diluted to a level of approximately 50/ml.
PubMed: 1690798
Affiliations:
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type="wicri:source">PubMed</idno><date when="1990">1990</date><idno type="RBID">pubmed:1690798</idno><idno type="pmid">1690798</idno><idno type="wicri:Area/PubMed/Corpus">005D91</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">005D91</idno><idno type="wicri:Area/PubMed/Curation">005D91</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">005D91</idno><idno type="wicri:Area/PubMed/Checkpoint">005D91</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">005D91</idno><idno type="wicri:Area/Ncbi/Merge">002365</idno><idno type="wicri:Area/Ncbi/Curation">002365</idno><idno type="wicri:Area/Ncbi/Checkpoint">002365</idno><idno type="wicri:doubleKey">0022-3395:1990:Long G:rapid:diagnosis:of</idno><idno type="wicri:Area/Main/Merge">00E559</idno><idno type="wicri:Area/Main/Curation">00D980</idno><idno type="wicri:Area/Main/Exploration">00D980</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Rapid diagnosis of Brugia malayi and Wuchereria bancrofti filariasis by an acridine orange/microhematocrit tube technique.</title><author><name sortKey="Long, G W" sort="Long, G W" uniqKey="Long G" first="G W" last="Long">G W Long</name><affiliation wicri:level="2"><nlm:affiliation>Infectious Diseases Department, Naval Medical Research Institute, Bethesda, Maryland 20814-5055.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Infectious Diseases Department, Naval Medical Research Institute, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Rickman, L S" sort="Rickman, L S" uniqKey="Rickman L" first="L S" last="Rickman">L S Rickman</name></author><author><name sortKey="Cross, J H" sort="Cross, J H" uniqKey="Cross J" first="J H" last="Cross">J H Cross</name></author></analytic><series><title level="j">The Journal of parasitology</title><idno type="ISSN">0022-3395</idno><imprint><date when="1990" type="published">1990</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acridine Orange</term><term>Animals</term><term>Brugia (isolation & purification)</term><term>Centrifugation</term><term>Elephantiasis, Filarial (diagnosis)</term><term>Filariasis (diagnosis)</term><term>Hematocrit</term><term>Humans</term><term>Microscopy, Fluorescence</term><term>Staining and Labeling</term><term>Wuchereria (isolation & purification)</term><term>Wuchereria bancrofti (isolation & purification)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Brugia (isolement et purification)</term><term>Centrifugation</term><term>Coloration et marquage</term><term>Filariose lymphatique (diagnostic)</term><term>Filarioses (diagnostic)</term><term>Humains</term><term>Hématocrite</term><term>Microscopie de fluorescence</term><term>Orange acridine</term><term>Wuchereria (isolement et purification)</term><term>Wuchereria bancrofti (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Acridine Orange</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term><term>Filariasis</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Filariose lymphatique</term><term>Filarioses</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Brugia</term><term>Wuchereria</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Brugia</term><term>Wuchereria</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Centrifugation</term><term>Hematocrit</term><term>Humans</term><term>Microscopy, Fluorescence</term><term>Staining and Labeling</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Centrifugation</term><term>Coloration et marquage</term><term>Humains</term><term>Hématocrite</term><term>Microscopie de fluorescence</term><term>Orange acridine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A microhematocrit tube technique for diagnosis of human filariasis has been previously described. A system incorporating heparin, EDTA, and acridine orange into a microhematocrit tube (Quantitative Blood Count, QBC) has been commercially developed for the quantitation of blood counts and has been used for the diagnosis of malaria. We evaluated this test for its usefulness in the diagnosis of filariasis. Upon centrifugation, the parasites were concentrated in the area of the buffy coat and could be observed through the wall of the tube. The parasites were concentrated further by a plastic float that expands the buffy coat and confines the parasites to the periphery of the tube. Acridine orange stains the DNA of the parasite, and morphologic characteristics can be examined by fluorescence microscopy. The terminal and subterminal nuclei and long cephalic space of Brugia malayi, as well as the short cephalic space and caudal nuclei of Wuchereria bancrofti, were easily recognized and differentiated from each other. Microfilariae were detected in samples diluted to a level of approximately 50/ml.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li></region></list><tree><noCountry><name sortKey="Cross, J H" sort="Cross, J H" uniqKey="Cross J" first="J H" last="Cross">J H Cross</name><name sortKey="Rickman, L S" sort="Rickman, L S" uniqKey="Rickman L" first="L S" last="Rickman">L S Rickman</name></noCountry><country name="États-Unis"><region name="Maryland"><name sortKey="Long, G W" sort="Long, G W" uniqKey="Long G" first="G W" last="Long">G W Long</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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