Rapid diagnosis of Brugia malayi and Wuchereria bancrofti filariasis by an acridine orange/microhematocrit tube technique.
Identifieur interne : 005D91 ( PubMed/Corpus ); précédent : 005D90; suivant : 005D92Rapid diagnosis of Brugia malayi and Wuchereria bancrofti filariasis by an acridine orange/microhematocrit tube technique.
Auteurs : G W Long ; L S Rickman ; J H CrossSource :
- The Journal of parasitology [ 0022-3395 ] ; 1990.
English descriptors
- KwdEn :
- MESH :
- chemical : Acridine Orange.
- diagnosis : Elephantiasis, Filarial, Filariasis.
- isolation & purification : Brugia, Wuchereria, Wuchereria bancrofti.
- Animals, Centrifugation, Hematocrit, Humans, Microscopy, Fluorescence, Staining and Labeling.
Abstract
A microhematocrit tube technique for diagnosis of human filariasis has been previously described. A system incorporating heparin, EDTA, and acridine orange into a microhematocrit tube (Quantitative Blood Count, QBC) has been commercially developed for the quantitation of blood counts and has been used for the diagnosis of malaria. We evaluated this test for its usefulness in the diagnosis of filariasis. Upon centrifugation, the parasites were concentrated in the area of the buffy coat and could be observed through the wall of the tube. The parasites were concentrated further by a plastic float that expands the buffy coat and confines the parasites to the periphery of the tube. Acridine orange stains the DNA of the parasite, and morphologic characteristics can be examined by fluorescence microscopy. The terminal and subterminal nuclei and long cephalic space of Brugia malayi, as well as the short cephalic space and caudal nuclei of Wuchereria bancrofti, were easily recognized and differentiated from each other. Microfilariae were detected in samples diluted to a level of approximately 50/ml.
PubMed: 1690798
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pubmed:1690798Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Rapid diagnosis of Brugia malayi and Wuchereria bancrofti filariasis by an acridine orange/microhematocrit tube technique.</title>
<author><name sortKey="Long, G W" sort="Long, G W" uniqKey="Long G" first="G W" last="Long">G W Long</name>
<affiliation><nlm:affiliation>Infectious Diseases Department, Naval Medical Research Institute, Bethesda, Maryland 20814-5055.</nlm:affiliation>
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<author><name sortKey="Rickman, L S" sort="Rickman, L S" uniqKey="Rickman L" first="L S" last="Rickman">L S Rickman</name>
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<author><name sortKey="Cross, J H" sort="Cross, J H" uniqKey="Cross J" first="J H" last="Cross">J H Cross</name>
</author>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Rapid diagnosis of Brugia malayi and Wuchereria bancrofti filariasis by an acridine orange/microhematocrit tube technique.</title>
<author><name sortKey="Long, G W" sort="Long, G W" uniqKey="Long G" first="G W" last="Long">G W Long</name>
<affiliation><nlm:affiliation>Infectious Diseases Department, Naval Medical Research Institute, Bethesda, Maryland 20814-5055.</nlm:affiliation>
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<author><name sortKey="Rickman, L S" sort="Rickman, L S" uniqKey="Rickman L" first="L S" last="Rickman">L S Rickman</name>
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<author><name sortKey="Cross, J H" sort="Cross, J H" uniqKey="Cross J" first="J H" last="Cross">J H Cross</name>
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<series><title level="j">The Journal of parasitology</title>
<idno type="ISSN">0022-3395</idno>
<imprint><date when="1990" type="published">1990</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acridine Orange</term>
<term>Animals</term>
<term>Brugia (isolation & purification)</term>
<term>Centrifugation</term>
<term>Elephantiasis, Filarial (diagnosis)</term>
<term>Filariasis (diagnosis)</term>
<term>Hematocrit</term>
<term>Humans</term>
<term>Microscopy, Fluorescence</term>
<term>Staining and Labeling</term>
<term>Wuchereria (isolation & purification)</term>
<term>Wuchereria bancrofti (isolation & purification)</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Acridine Orange</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term>
<term>Filariasis</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Brugia</term>
<term>Wuchereria</term>
<term>Wuchereria bancrofti</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Centrifugation</term>
<term>Hematocrit</term>
<term>Humans</term>
<term>Microscopy, Fluorescence</term>
<term>Staining and Labeling</term>
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<front><div type="abstract" xml:lang="en">A microhematocrit tube technique for diagnosis of human filariasis has been previously described. A system incorporating heparin, EDTA, and acridine orange into a microhematocrit tube (Quantitative Blood Count, QBC) has been commercially developed for the quantitation of blood counts and has been used for the diagnosis of malaria. We evaluated this test for its usefulness in the diagnosis of filariasis. Upon centrifugation, the parasites were concentrated in the area of the buffy coat and could be observed through the wall of the tube. The parasites were concentrated further by a plastic float that expands the buffy coat and confines the parasites to the periphery of the tube. Acridine orange stains the DNA of the parasite, and morphologic characteristics can be examined by fluorescence microscopy. The terminal and subterminal nuclei and long cephalic space of Brugia malayi, as well as the short cephalic space and caudal nuclei of Wuchereria bancrofti, were easily recognized and differentiated from each other. Microfilariae were detected in samples diluted to a level of approximately 50/ml.</div>
</front>
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<DateCreated><Year>1990</Year>
<Month>05</Month>
<Day>04</Day>
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<DateCompleted><Year>1990</Year>
<Month>05</Month>
<Day>04</Day>
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<DateRevised><Year>2013</Year>
<Month>11</Month>
<Day>21</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-3395</ISSN>
<JournalIssue CitedMedium="Print"><Volume>76</Volume>
<Issue>2</Issue>
<PubDate><Year>1990</Year>
<Month>Apr</Month>
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<Title>The Journal of parasitology</Title>
<ISOAbbreviation>J. Parasitol.</ISOAbbreviation>
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<ArticleTitle>Rapid diagnosis of Brugia malayi and Wuchereria bancrofti filariasis by an acridine orange/microhematocrit tube technique.</ArticleTitle>
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<Abstract><AbstractText>A microhematocrit tube technique for diagnosis of human filariasis has been previously described. A system incorporating heparin, EDTA, and acridine orange into a microhematocrit tube (Quantitative Blood Count, QBC) has been commercially developed for the quantitation of blood counts and has been used for the diagnosis of malaria. We evaluated this test for its usefulness in the diagnosis of filariasis. Upon centrifugation, the parasites were concentrated in the area of the buffy coat and could be observed through the wall of the tube. The parasites were concentrated further by a plastic float that expands the buffy coat and confines the parasites to the periphery of the tube. Acridine orange stains the DNA of the parasite, and morphologic characteristics can be examined by fluorescence microscopy. The terminal and subterminal nuclei and long cephalic space of Brugia malayi, as well as the short cephalic space and caudal nuclei of Wuchereria bancrofti, were easily recognized and differentiated from each other. Microfilariae were detected in samples diluted to a level of approximately 50/ml.</AbstractText>
</Abstract>
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<MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
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<MeshHeading><DescriptorName UI="D002009" MajorTopicYN="N">Brugia</DescriptorName>
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<MeshHeading><DescriptorName UI="D002498" MajorTopicYN="N">Centrifugation</DescriptorName>
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<MeshHeading><DescriptorName UI="D004605" MajorTopicYN="N">Elephantiasis, Filarial</DescriptorName>
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<MeshHeading><DescriptorName UI="D005368" MajorTopicYN="N">Filariasis</DescriptorName>
<QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName>
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<MeshHeading><DescriptorName UI="D006400" MajorTopicYN="N">Hematocrit</DescriptorName>
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<MeshHeading><DescriptorName UI="D013194" MajorTopicYN="N">Staining and Labeling</DescriptorName>
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<MeshHeading><DescriptorName UI="D014957" MajorTopicYN="N">Wuchereria</DescriptorName>
<QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName>
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<MeshHeading><DescriptorName UI="D014958" MajorTopicYN="N">Wuchereria bancrofti</DescriptorName>
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