Using an intravenous catheter to carry out abdominal lavage in the gerbil.
Identifieur interne : 005530 ( Main/Exploration ); précédent : 005529; suivant : 005531Using an intravenous catheter to carry out abdominal lavage in the gerbil.
Auteurs : Kathryn G. Griffiths [États-Unis] ; Leanne C. Alworth ; Stephen B. Harvey ; Michelle L. MichalskiSource :
- Lab animal [ 0093-7355 ] ; 2010.
Descripteurs français
- KwdFr :
- Animaux, Animaux de laboratoire, Brugia (isolement et purification), Cathétérisme (médecine vétérinaire), Filariose lymphatique (parasitologie), Gerbillinae (physiologie), Lavage péritonéal (), Lavage péritonéal (instrumentation), Modèles animaux de maladie humaine, Mâle, Parasitoses animales (parasitologie), Prélèvement biologique, Sciences des animaux de laboratoire (), Sciences des animaux de laboratoire (instrumentation).
- MESH :
- isolement et purification : Brugia.
- médecine vétérinaire : Cathétérisme.
- parasitologie : Filariose lymphatique, Parasitoses animales.
- physiologie : Gerbillinae.
- instrumentation : Animaux, Animaux de laboratoire, Lavage péritonéal, Modèles animaux de maladie humaine, Mâle, Prélèvement biologique, Sciences des animaux de laboratoire.
English descriptors
- KwdEn :
- Animals, Animals, Laboratory, Brugia (isolation & purification), Catheterization (veterinary), Disease Models, Animal, Elephantiasis, Filarial (parasitology), Gerbillinae (physiology), Laboratory Animal Science (instrumentation), Laboratory Animal Science (methods), Male, Parasitic Diseases, Animal (parasitology), Peritoneal Lavage (instrumentation), Peritoneal Lavage (methods), Specimen Handling.
- MESH :
- instrumentation : Laboratory Animal Science, Peritoneal Lavage.
- isolation & purification : Brugia.
- methods : Laboratory Animal Science, Peritoneal Lavage.
- parasitology : Elephantiasis, Filarial, Parasitic Diseases, Animal.
- physiology : Gerbillinae.
- veterinary : Catheterization.
- Animals, Animals, Laboratory, Disease Models, Animal, Male, Specimen Handling.
Abstract
Abdominal lavage is used in laboratory rodents for a variety of applications but carries an inherent risk of abdominal organ laceration; therefore, personnel carrying out this procedure must have considerable expertise. In this paper, the authors describe an improved method for delivering sterile media to and collecting peritoneal fluids from dark-clawed Mongolian gerbils (Meriones unguiculatus) that had been peritoneally infected with filarial nematode parasites (genus Brugia). To carry out this gravity-assisted technique, the authors used a catheter to introduce sterile media into the peritoneal cavity of each gerbil and then to passively drain peritoneal fluid and larval worms for collection. Average fluid recovery was consistently greater when using this gravity-assisted method than when using aspiration. Larval parasites were recovered by both methods. To recover large volumes of fluid using the standard method of abdominal lavage, personnel typically must euthanize rodents. This gravity-assisted technique allows researchers to collect large numbers of parasite larvae without euthanizing gerbils.
DOI: 10.1038/laban0510-143
PubMed: 20410898
Affiliations:
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Griffiths</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biology and Microbiology, University of Wisconsin-Oshkosh, Oshkosh, WI, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biology and Microbiology, University of Wisconsin-Oshkosh, Oshkosh, WI</wicri:regionArea><placeName><region type="state">Wisconsin</region></placeName></affiliation></author><author><name sortKey="Alworth, Leanne C" sort="Alworth, Leanne C" uniqKey="Alworth L" first="Leanne C" last="Alworth">Leanne C. Alworth</name></author><author><name sortKey="Harvey, Stephen B" sort="Harvey, Stephen B" uniqKey="Harvey S" first="Stephen B" last="Harvey">Stephen B. Harvey</name></author><author><name sortKey="Michalski, Michelle L" sort="Michalski, Michelle L" uniqKey="Michalski M" first="Michelle L" last="Michalski">Michelle L. 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In this paper, the authors describe an improved method for delivering sterile media to and collecting peritoneal fluids from dark-clawed Mongolian gerbils (Meriones unguiculatus) that had been peritoneally infected with filarial nematode parasites (genus Brugia). To carry out this gravity-assisted technique, the authors used a catheter to introduce sterile media into the peritoneal cavity of each gerbil and then to passively drain peritoneal fluid and larval worms for collection. Average fluid recovery was consistently greater when using this gravity-assisted method than when using aspiration. Larval parasites were recovered by both methods. To recover large volumes of fluid using the standard method of abdominal lavage, personnel typically must euthanize rodents. This gravity-assisted technique allows researchers to collect large numbers of parasite larvae without euthanizing gerbils.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Wisconsin</li></region></list><tree><noCountry><name sortKey="Alworth, Leanne C" sort="Alworth, Leanne C" uniqKey="Alworth L" first="Leanne C" last="Alworth">Leanne C. Alworth</name><name sortKey="Harvey, Stephen B" sort="Harvey, Stephen B" uniqKey="Harvey S" first="Stephen B" last="Harvey">Stephen B. Harvey</name><name sortKey="Michalski, Michelle L" sort="Michalski, Michelle L" uniqKey="Michalski M" first="Michelle L" last="Michalski">Michelle L. Michalski</name></noCountry><country name="États-Unis"><region name="Wisconsin"><name sortKey="Griffiths, Kathryn G" sort="Griffiths, Kathryn G" uniqKey="Griffiths K" first="Kathryn G" last="Griffiths">Kathryn G. Griffiths</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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