LymphedemaV1, PubMed, Corpus, bibRecord, 002B01

Using an intravenous catheter to carry out abdominal lavage in the gerbil.

Identifieur interne : 002B01 ( PubMed/Corpus ); précédent : 002B00; suivant : 002B02

Using an intravenous catheter to carry out abdominal lavage in the gerbil.

Auteurs : Kathryn G. Griffiths ; Leanne C. Alworth ; Stephen B. Harvey ; Michelle L. Michalski

Source :

Lab animal [ 0093-7355 ] ; 2010.

RBID : pubmed:20410898

English descriptors

KwdEn :
- Animals, Animals, Laboratory, Brugia (isolation & purification), Catheterization (veterinary), Disease Models, Animal, Elephantiasis, Filarial (parasitology), Gerbillinae (physiology), Laboratory Animal Science (instrumentation), Laboratory Animal Science (methods), Male, Parasitic Diseases, Animal (parasitology), Peritoneal Lavage (instrumentation), Peritoneal Lavage (methods), Specimen Handling.
MESH :
- instrumentation : Laboratory Animal Science, Peritoneal Lavage.
- isolation & purification : Brugia.
- methods : Laboratory Animal Science, Peritoneal Lavage.
- parasitology : Elephantiasis, Filarial, Parasitic Diseases, Animal.
- physiology : Gerbillinae.
- veterinary : Catheterization.
- Animals, Animals, Laboratory, Disease Models, Animal, Male, Specimen Handling.

Abstract

Abdominal lavage is used in laboratory rodents for a variety of applications but carries an inherent risk of abdominal organ laceration; therefore, personnel carrying out this procedure must have considerable expertise. In this paper, the authors describe an improved method for delivering sterile media to and collecting peritoneal fluids from dark-clawed Mongolian gerbils (Meriones unguiculatus) that had been peritoneally infected with filarial nematode parasites (genus Brugia). To carry out this gravity-assisted technique, the authors used a catheter to introduce sterile media into the peritoneal cavity of each gerbil and then to passively drain peritoneal fluid and larval worms for collection. Average fluid recovery was consistently greater when using this gravity-assisted method than when using aspiration. Larval parasites were recovered by both methods. To recover large volumes of fluid using the standard method of abdominal lavage, personnel typically must euthanize rodents. This gravity-assisted technique allows researchers to collect large numbers of parasite larvae without euthanizing gerbils.

DOI: 10.1038/laban0510-143
PubMed: 20410898

Links to Exploration step

pubmed:20410898

Le document en format XML

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<author><name sortKey="Griffiths, Kathryn G" sort="Griffiths, Kathryn G" uniqKey="Griffiths K" first="Kathryn G" last="Griffiths">Kathryn G. Griffiths</name>
<affiliation><nlm:affiliation>Department of Biology and Microbiology, University of Wisconsin-Oshkosh, Oshkosh, WI, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Alworth, Leanne C" sort="Alworth, Leanne C" uniqKey="Alworth L" first="Leanne C" last="Alworth">Leanne C. Alworth</name>
</author>
<author><name sortKey="Harvey, Stephen B" sort="Harvey, Stephen B" uniqKey="Harvey S" first="Stephen B" last="Harvey">Stephen B. Harvey</name>
</author>
<author><name sortKey="Michalski, Michelle L" sort="Michalski, Michelle L" uniqKey="Michalski M" first="Michelle L" last="Michalski">Michelle L. Michalski</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Using an intravenous catheter to carry out abdominal lavage in the gerbil.</title>
<author><name sortKey="Griffiths, Kathryn G" sort="Griffiths, Kathryn G" uniqKey="Griffiths K" first="Kathryn G" last="Griffiths">Kathryn G. Griffiths</name>
<affiliation><nlm:affiliation>Department of Biology and Microbiology, University of Wisconsin-Oshkosh, Oshkosh, WI, USA.</nlm:affiliation>
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<author><name sortKey="Alworth, Leanne C" sort="Alworth, Leanne C" uniqKey="Alworth L" first="Leanne C" last="Alworth">Leanne C. Alworth</name>
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<author><name sortKey="Harvey, Stephen B" sort="Harvey, Stephen B" uniqKey="Harvey S" first="Stephen B" last="Harvey">Stephen B. Harvey</name>
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<author><name sortKey="Michalski, Michelle L" sort="Michalski, Michelle L" uniqKey="Michalski M" first="Michelle L" last="Michalski">Michelle L. Michalski</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Animals, Laboratory</term>
<term>Brugia (isolation & purification)</term>
<term>Catheterization (veterinary)</term>
<term>Disease Models, Animal</term>
<term>Elephantiasis, Filarial (parasitology)</term>
<term>Gerbillinae (physiology)</term>
<term>Laboratory Animal Science (instrumentation)</term>
<term>Laboratory Animal Science (methods)</term>
<term>Male</term>
<term>Parasitic Diseases, Animal (parasitology)</term>
<term>Peritoneal Lavage (instrumentation)</term>
<term>Peritoneal Lavage (methods)</term>
<term>Specimen Handling</term>
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<keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Laboratory Animal Science</term>
<term>Peritoneal Lavage</term>
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<term>Peritoneal Lavage</term>
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<keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Elephantiasis, Filarial</term>
<term>Parasitic Diseases, Animal</term>
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<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Gerbillinae</term>
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<keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Catheterization</term>
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<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
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<front><div type="abstract" xml:lang="en">Abdominal lavage is used in laboratory rodents for a variety of applications but carries an inherent risk of abdominal organ laceration; therefore, personnel carrying out this procedure must have considerable expertise. In this paper, the authors describe an improved method for delivering sterile media to and collecting peritoneal fluids from dark-clawed Mongolian gerbils (Meriones unguiculatus) that had been peritoneally infected with filarial nematode parasites (genus Brugia). To carry out this gravity-assisted technique, the authors used a catheter to introduce sterile media into the peritoneal cavity of each gerbil and then to passively drain peritoneal fluid and larval worms for collection. Average fluid recovery was consistently greater when using this gravity-assisted method than when using aspiration. Larval parasites were recovered by both methods. To recover large volumes of fluid using the standard method of abdominal lavage, personnel typically must euthanize rodents. This gravity-assisted technique allows researchers to collect large numbers of parasite larvae without euthanizing gerbils.</div>
</front>
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<Month>04</Month>
<Day>22</Day>
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<DateCompleted><Year>2010</Year>
<Month>06</Month>
<Day>15</Day>
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<DateRevised><Year>2010</Year>
<Month>04</Month>
<Day>22</Day>
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<JournalIssue CitedMedium="Print"><Volume>39</Volume>
<Issue>5</Issue>
<PubDate><Year>2010</Year>
<Month>May</Month>
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<Title>Lab animal</Title>
<ISOAbbreviation>Lab Anim (NY)</ISOAbbreviation>
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<ArticleTitle>Using an intravenous catheter to carry out abdominal lavage in the gerbil.</ArticleTitle>
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<Abstract><AbstractText>Abdominal lavage is used in laboratory rodents for a variety of applications but carries an inherent risk of abdominal organ laceration; therefore, personnel carrying out this procedure must have considerable expertise. In this paper, the authors describe an improved method for delivering sterile media to and collecting peritoneal fluids from dark-clawed Mongolian gerbils (Meriones unguiculatus) that had been peritoneally infected with filarial nematode parasites (genus Brugia). To carry out this gravity-assisted technique, the authors used a catheter to introduce sterile media into the peritoneal cavity of each gerbil and then to passively drain peritoneal fluid and larval worms for collection. Average fluid recovery was consistently greater when using this gravity-assisted method than when using aspiration. Larval parasites were recovered by both methods. To recover large volumes of fluid using the standard method of abdominal lavage, personnel typically must euthanize rodents. This gravity-assisted technique allows researchers to collect large numbers of parasite larvae without euthanizing gerbils.</AbstractText>
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<MeshHeading><DescriptorName UI="D010273" MajorTopicYN="N">Parasitic Diseases, Animal</DescriptorName>
<QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010533" MajorTopicYN="N">Peritoneal Lavage</DescriptorName>
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<QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013048" MajorTopicYN="N">Specimen Handling</DescriptorName>
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<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2009</Year>
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Using an intravenous catheter to carry out abdominal lavage in the gerbil.

Using an intravenous catheter to carry out abdominal lavage in the gerbil.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki