Therapeutic lymphangiogenesis using stem cell and VEGF-C hydrogel.
Identifieur interne : 004A39 ( Main/Exploration ); précédent : 004A38; suivant : 004A40Therapeutic lymphangiogenesis using stem cell and VEGF-C hydrogel.
Auteurs : Ji Hye Hwang [Corée du Sud] ; In Gul Kim ; Ji Young Lee ; Shuyu Piao ; David S. Lee ; Tae Seung Lee ; Jeong Chan Ra ; Ji Youl LeeSource :
- Biomaterials [ 1878-5905 ] ; 2011.
Descripteurs français
- KwdFr :
- Animaux, Cellules souches (cytologie), Cellules souches (physiologie), Facteur de croissance endothéliale vasculaire de type C (pharmacologie), Femelle, Gélatine (), Humains, Hydrogel (), Hydrogel (pharmacologie), Lymphangiogenèse, Lymphoedème (), Matériaux biocompatibles (), Matériaux biocompatibles (métabolisme), Matériaux biocompatibles (usage thérapeutique), Modèles animaux de maladie humaine, Régénération (physiologie), Souris, Souris de lignée BALB C, Test de matériaux, Transplantation de cellules souches.
- MESH :
- cytologie : Cellules souches.
- métabolisme : Matériaux biocompatibles.
- pharmacologie : Facteur de croissance endothéliale vasculaire de type C, Hydrogel.
- physiologie : Cellules souches, Régénération.
- usage thérapeutique : Matériaux biocompatibles.
- Animaux, Femelle, Gélatine, Humains, Hydrogel, Lymphangiogenèse, Lymphoedème, Matériaux biocompatibles, Modèles animaux de maladie humaine, Souris, Souris de lignée BALB C, Test de matériaux, Transplantation de cellules souches.
English descriptors
- KwdEn :
- Animals, Biocompatible Materials (chemistry), Biocompatible Materials (metabolism), Biocompatible Materials (therapeutic use), Disease Models, Animal, Female, Gelatin (chemistry), Humans, Hydrogel (chemistry), Hydrogel (pharmacology), Lymphangiogenesis, Lymphedema (therapy), Materials Testing, Mice, Mice, Inbred BALB C, Regeneration (physiology), Stem Cell Transplantation, Stem Cells (cytology), Stem Cells (physiology), Vascular Endothelial Growth Factor C (pharmacology).
- MESH :
- chemical , chemistry : Biocompatible Materials, Gelatin, Hydrogel.
- chemical , metabolism : Biocompatible Materials.
- chemical , pharmacology : Hydrogel, Vascular Endothelial Growth Factor C.
- chemical , therapeutic use : Biocompatible Materials.
- cytology : Stem Cells.
- physiology : Regeneration, Stem Cells.
- therapy : Lymphedema.
- Animals, Disease Models, Animal, Female, Humans, Lymphangiogenesis, Materials Testing, Mice, Mice, Inbred BALB C, Stem Cell Transplantation.
Abstract
Lymphedema is a manifestation of lymphatic system insufficiency. It arises from primary lymphatic dysplasia or secondary obliteration after lymph node dissection or irradiation. Although improvement of swelling can be achieved by comprehensive non-operative therapy, treatment of this condition requires lifelong care and good compliance. Recently molecular-based treatments using VEGF-C have been investigated by several researchers. We designed the present study to determine whether the therapeutic efficacy of implanted human adipose-derived stem cells (hADSCs) could be improved by applying a gelatin hydrogel containing VEGF-C (VEGF-C hydrogel) to the site of tissue injury in a lymphedema mouse model. Four weeks after the operation, we evaluated edema and determined lymphatic vessel density at various post-operative time points. Mice treated with hADSCs and VEGF-C hydrogel showed a significantly decreased dermal edema depth compared to the groups of mice that received hADSCs only or VEGF-C hydrogel only. Immunohistochemical analysis also revealed that the hADSC/VEGF-C hydrogel group showed significantly greater lymphatic vessel regeneration than all the other groups. hADSCs were detected in the implantation sites of all mice in the hADSC/VEGF-C group, and exhibited a lymphatic endothelial differentiation phenotype as determined by co-staining PKH-labeled hADSCs for the lymphatic marker LYVE-1. Our results suggest that co-administration of hADSCs and VEGF-C hydrogel has a substantial positive effect on lymphangiogenesis.
DOI: 10.1016/j.biomaterials.2011.02.051
PubMed: 21421266
Affiliations:
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Lee</name></author><author><name sortKey="Lee, Tae Seung" sort="Lee, Tae Seung" uniqKey="Lee T" first="Tae Seung" last="Lee">Tae Seung Lee</name></author><author><name sortKey="Ra, Jeong Chan" sort="Ra, Jeong Chan" uniqKey="Ra J" first="Jeong Chan" last="Ra">Jeong Chan Ra</name></author><author><name sortKey="Lee, Ji Youl" sort="Lee, Ji Youl" uniqKey="Lee J" first="Ji Youl" last="Lee">Ji Youl Lee</name></author></analytic><series><title level="j">Biomaterials</title><idno type="eISSN">1878-5905</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Biocompatible Materials (chemistry)</term><term>Biocompatible Materials (metabolism)</term><term>Biocompatible Materials (therapeutic use)</term><term>Disease Models, Animal</term><term>Female</term><term>Gelatin (chemistry)</term><term>Humans</term><term>Hydrogel (chemistry)</term><term>Hydrogel (pharmacology)</term><term>Lymphangiogenesis</term><term>Lymphedema (therapy)</term><term>Materials Testing</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Regeneration (physiology)</term><term>Stem Cell Transplantation</term><term>Stem Cells (cytology)</term><term>Stem Cells (physiology)</term><term>Vascular Endothelial Growth Factor C (pharmacology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules souches (cytologie)</term><term>Cellules souches (physiologie)</term><term>Facteur de croissance endothéliale vasculaire de type C (pharmacologie)</term><term>Femelle</term><term>Gélatine ()</term><term>Humains</term><term>Hydrogel ()</term><term>Hydrogel (pharmacologie)</term><term>Lymphangiogenèse</term><term>Lymphoedème ()</term><term>Matériaux biocompatibles ()</term><term>Matériaux biocompatibles (métabolisme)</term><term>Matériaux biocompatibles (usage thérapeutique)</term><term>Modèles animaux de maladie humaine</term><term>Régénération (physiologie)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Test de matériaux</term><term>Transplantation de cellules souches</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Biocompatible Materials</term><term>Gelatin</term><term>Hydrogel</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Biocompatible Materials</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Hydrogel</term><term>Vascular Endothelial Growth Factor C</term></keywords><keywords scheme="MESH" type="chemical" qualifier="therapeutic use" xml:lang="en"><term>Biocompatible Materials</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Cellules souches</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Stem Cells</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Matériaux biocompatibles</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Facteur de croissance endothéliale vasculaire de type C</term><term>Hydrogel</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Cellules souches</term><term>Régénération</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Regeneration</term><term>Stem Cells</term></keywords><keywords scheme="MESH" qualifier="therapy" xml:lang="en"><term>Lymphedema</term></keywords><keywords scheme="MESH" qualifier="usage thérapeutique" xml:lang="fr"><term>Matériaux biocompatibles</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Disease Models, Animal</term><term>Female</term><term>Humans</term><term>Lymphangiogenesis</term><term>Materials Testing</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Stem Cell Transplantation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Femelle</term><term>Gélatine</term><term>Humains</term><term>Hydrogel</term><term>Lymphangiogenèse</term><term>Lymphoedème</term><term>Matériaux biocompatibles</term><term>Modèles animaux de maladie humaine</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Test de matériaux</term><term>Transplantation de cellules souches</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Lymphedema is a manifestation of lymphatic system insufficiency. It arises from primary lymphatic dysplasia or secondary obliteration after lymph node dissection or irradiation. Although improvement of swelling can be achieved by comprehensive non-operative therapy, treatment of this condition requires lifelong care and good compliance. Recently molecular-based treatments using VEGF-C have been investigated by several researchers. We designed the present study to determine whether the therapeutic efficacy of implanted human adipose-derived stem cells (hADSCs) could be improved by applying a gelatin hydrogel containing VEGF-C (VEGF-C hydrogel) to the site of tissue injury in a lymphedema mouse model. Four weeks after the operation, we evaluated edema and determined lymphatic vessel density at various post-operative time points. Mice treated with hADSCs and VEGF-C hydrogel showed a significantly decreased dermal edema depth compared to the groups of mice that received hADSCs only or VEGF-C hydrogel only. Immunohistochemical analysis also revealed that the hADSC/VEGF-C hydrogel group showed significantly greater lymphatic vessel regeneration than all the other groups. hADSCs were detected in the implantation sites of all mice in the hADSC/VEGF-C group, and exhibited a lymphatic endothelial differentiation phenotype as determined by co-staining PKH-labeled hADSCs for the lymphatic marker LYVE-1. Our results suggest that co-administration of hADSCs and VEGF-C hydrogel has a substantial positive effect on lymphangiogenesis.</div></front></TEI><affiliations><list><country><li>Corée du Sud</li></country><region><li>Région capitale de Séoul</li></region><settlement><li>Séoul</li></settlement></list><tree><noCountry><name sortKey="Kim, In Gul" sort="Kim, In Gul" uniqKey="Kim I" first="In Gul" last="Kim">In Gul Kim</name><name sortKey="Lee, David S" sort="Lee, David S" uniqKey="Lee D" first="David S" last="Lee">David S. Lee</name><name sortKey="Lee, Ji Youl" sort="Lee, Ji Youl" uniqKey="Lee J" first="Ji Youl" last="Lee">Ji Youl Lee</name><name sortKey="Lee, Ji Young" sort="Lee, Ji Young" uniqKey="Lee J" first="Ji Young" last="Lee">Ji Young Lee</name><name sortKey="Lee, Tae Seung" sort="Lee, Tae Seung" uniqKey="Lee T" first="Tae Seung" last="Lee">Tae Seung Lee</name><name sortKey="Piao, Shuyu" sort="Piao, Shuyu" uniqKey="Piao S" first="Shuyu" last="Piao">Shuyu Piao</name><name sortKey="Ra, Jeong Chan" sort="Ra, Jeong Chan" uniqKey="Ra J" first="Jeong Chan" last="Ra">Jeong Chan Ra</name></noCountry><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Hwang, Ji Hye" sort="Hwang, Ji Hye" uniqKey="Hwang J" first="Ji Hye" last="Hwang">Ji Hye Hwang</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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