LymphedemaV1, Main, Exploration, bibRecord, 003661

Evaluation of PCR-ELISA as a tool for monitoring transmission of Wuchereria bancrofti in District of Gampaha, Sri Lanka

Identifieur interne : 003661 ( Main/Exploration ); précédent : 003660; suivant : 003662

Evaluation of PCR-ELISA as a tool for monitoring transmission of Wuchereria bancrofti in District of Gampaha, Sri Lanka

Auteurs : Asha Dilrukshi Wijegunawardana [Sri Lanka] ; Nilmini Silva Gunawardane [Sri Lanka] ; Chanditha Hapuarachchi [Singapour] ; Aresha Manamperi [Sri Lanka] ; Kithsiri Gunawardena [Sri Lanka] ; Wimaladharma Abeyewickrama [Sri Lanka]

Source :

Asian Pacific Journal of Tropical Biomedicine [ 2221-1691 ] ; 2013.

RBID : PMC:3642448

Descripteurs français

KwdFr :
- Animaux, Culicidae (parasitologie), Filariose lymphatique (transmission), Filariose lymphatique (épidémiologie), Humains, Prévalence, Réaction de polymérisation en chaîne, Sri Lanka (épidémiologie), Surveillance de la population, Test ELISA, Wuchereria bancrofti (génétique), Wuchereria bancrofti (immunologie).
MESH :
- génétique : Wuchereria bancrofti.
- immunologie : Wuchereria bancrofti.
- parasitologie : Culicidae.
- épidémiologie : Filariose lymphatique, Sri Lanka.
- Animaux, Humains, Prévalence, Réaction de polymérisation en chaîne, Surveillance de la population, Test ELISA.
Wicri :
- geographic : Sri Lanka.

English descriptors

KwdEn :
- Animals, Culicidae (parasitology), Elephantiasis, Filarial (epidemiology), Elephantiasis, Filarial (transmission), Enzyme-Linked Immunosorbent Assay, Humans, Polymerase Chain Reaction, Population Surveillance, Prevalence, Sri Lanka (epidemiology), Wuchereria bancrofti (genetics), Wuchereria bancrofti (immunology).
MESH :
- geographic , epidemiology : Sri Lanka.
- epidemiology : Elephantiasis, Filarial.
- genetics : Wuchereria bancrofti.
- immunology : Wuchereria bancrofti.
- parasitology : Culicidae.
- transmission : Elephantiasis, Filarial.
- Animals, Enzyme-Linked Immunosorbent Assay, Humans, Polymerase Chain Reaction, Population Surveillance, Prevalence.

Abstract

Objective

To compare Wuchereria bancrofti (W. bancrofti) infection rates of Culex quinquefasciatus, using dissection and PCR-ELISA in two consecutive time periods (from 2007 to 2008 and from 2008 to 2009).

Methods

Mosquitoes were collected in 30 sentinel and 15 non-sentinel sites in 15 Medical Officer of Health areas of Gampaha District known for the presence of W. bancrofti transmission in two consecutive time period of 2007 to 2008 and 2008 to 2009. Captured mosquitoes were dissected to determine the W. bancrofti larvae (L₁, L₂, L₃). PCR was carried out using DNA extracted from mosquito pools (15 body parts/pool) utilizing the primers specific for Wb-SspI repeat. PCR products were analyzed by hybridization ELISA using fluorescein-labeled wild type specific probes. The prevalence of infected/infective mosquitoes in PCR pools (3 pools/site) was estimated using the PoolScreen™ algorithm and a novel probability-based method.

Results

Of 45 batches of mosquitoes dissected, W. bancrofti infected mosquitoes were found in 19 and 13 batches, with an infection rate of 13.29% and 3.10% with mean larval density of 8.7 and 1.0 larvae per mosquito for two study periods in the Gampaha District. Total of 405 pools of head, thorax and abdomen were processed by PCR-ELISA for each year. Of these, 51 and 31 pools were positive for W. bancrofti in the two study periods respectively. The association of dissection based prevalence rates with PCR based rates as determined by the Pearson correlation coefficient were 0.176 and 0.890 respectively for the two periods.

Conclusions

Data indicate that PCR-ELISA is more sensitive than the traditional dissection techniques for monitoring transmission intensity.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3642448

DOI: 10.1016/S2221-1691(13)60081-7
PubMed: 23646302
PubMed Central: 3642448

Affiliations:

Singapour, Sri Lanka

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Evaluation of PCR-ELISA as a tool for monitoring transmission of <italic>Wuchereria bancrofti</italic>
 in District of Gampaha, Sri Lanka</title>
<author><name sortKey="Wijegunawardana, Asha Dilrukshi" sort="Wijegunawardana, Asha Dilrukshi" uniqKey="Wijegunawardana A" first="Asha Dilrukshi" last="Wijegunawardana">Asha Dilrukshi Wijegunawardana</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka</addr-line>
</nlm:aff>
<country xml:lang="fr">Sri Lanka</country>
<wicri:regionArea>Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama</wicri:regionArea>
<wicri:noRegion>Ragama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Gunawardane, Nilmini Silva" sort="Gunawardane, Nilmini Silva" uniqKey="Gunawardane N" first="Nilmini Silva" last="Gunawardane">Nilmini Silva Gunawardane</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka</addr-line>
</nlm:aff>
<country xml:lang="fr">Sri Lanka</country>
<wicri:regionArea>Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama</wicri:regionArea>
<wicri:noRegion>Ragama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hapuarachchi, Chanditha" sort="Hapuarachchi, Chanditha" uniqKey="Hapuarachchi C" first="Chanditha" last="Hapuarachchi">Chanditha Hapuarachchi</name>
<affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Environmental Authority, Singapore</addr-line>
</nlm:aff>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>Environmental Authority</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Manamperi, Aresha" sort="Manamperi, Aresha" uniqKey="Manamperi A" first="Aresha" last="Manamperi">Aresha Manamperi</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka</addr-line>
</nlm:aff>
<country xml:lang="fr">Sri Lanka</country>
<wicri:regionArea>Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama</wicri:regionArea>
<wicri:noRegion>Ragama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Gunawardena, Kithsiri" sort="Gunawardena, Kithsiri" uniqKey="Gunawardena K" first="Kithsiri" last="Gunawardena">Kithsiri Gunawardena</name>
<affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Department of Parasitology, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka</addr-line>
</nlm:aff>
<country xml:lang="fr">Sri Lanka</country>
<wicri:regionArea>Department of Parasitology, Faculty of Medicine, University of Kelaniya, Ragama</wicri:regionArea>
<wicri:noRegion>Ragama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Abeyewickrama, Wimaladharma" sort="Abeyewickrama, Wimaladharma" uniqKey="Abeyewickrama W" first="Wimaladharma" last="Abeyewickrama">Wimaladharma Abeyewickrama</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka</addr-line>
</nlm:aff>
<country xml:lang="fr">Sri Lanka</country>
<wicri:regionArea>Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama</wicri:regionArea>
<wicri:noRegion>Ragama</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Department of Parasitology, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka</addr-line>
</nlm:aff>
<country xml:lang="fr">Sri Lanka</country>
<wicri:regionArea>Department of Parasitology, Faculty of Medicine, University of Kelaniya, Ragama</wicri:regionArea>
<wicri:noRegion>Ragama</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">23646302</idno>
<idno type="pmc">3642448</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3642448</idno>
<idno type="RBID">PMC:3642448</idno>
<idno type="doi">10.1016/S2221-1691(13)60081-7</idno>
<date when="2013">2013</date>
<idno type="wicri:Area/Pmc/Corpus">002C48</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002C48</idno>
<idno type="wicri:Area/Pmc/Curation">002C47</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">002C47</idno>
<idno type="wicri:Area/Pmc/Checkpoint">002346</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">002346</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="wicri:Area/PubMed/Corpus">001B63</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001B63</idno>
<idno type="wicri:Area/PubMed/Curation">001B63</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001B63</idno>
<idno type="wicri:Area/PubMed/Checkpoint">001B63</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001B63</idno>
<idno type="wicri:Area/Ncbi/Merge">005892</idno>
<idno type="wicri:Area/Ncbi/Curation">005892</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">005892</idno>
<idno type="wicri:doubleKey">2221-1691:2013:Wijegunawardana A:evaluation:of:pcr</idno>
<idno type="wicri:Area/Main/Merge">003668</idno>
<idno type="wicri:Area/Main/Curation">003661</idno>
<idno type="wicri:Area/Main/Exploration">003661</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Evaluation of PCR-ELISA as a tool for monitoring transmission of <italic>Wuchereria bancrofti</italic>
 in District of Gampaha, Sri Lanka</title>
<author><name sortKey="Wijegunawardana, Asha Dilrukshi" sort="Wijegunawardana, Asha Dilrukshi" uniqKey="Wijegunawardana A" first="Asha Dilrukshi" last="Wijegunawardana">Asha Dilrukshi Wijegunawardana</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka</addr-line>
</nlm:aff>
<country xml:lang="fr">Sri Lanka</country>
<wicri:regionArea>Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama</wicri:regionArea>
<wicri:noRegion>Ragama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Gunawardane, Nilmini Silva" sort="Gunawardane, Nilmini Silva" uniqKey="Gunawardane N" first="Nilmini Silva" last="Gunawardane">Nilmini Silva Gunawardane</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka</addr-line>
</nlm:aff>
<country xml:lang="fr">Sri Lanka</country>
<wicri:regionArea>Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama</wicri:regionArea>
<wicri:noRegion>Ragama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hapuarachchi, Chanditha" sort="Hapuarachchi, Chanditha" uniqKey="Hapuarachchi C" first="Chanditha" last="Hapuarachchi">Chanditha Hapuarachchi</name>
<affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Environmental Authority, Singapore</addr-line>
</nlm:aff>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea>Environmental Authority</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Manamperi, Aresha" sort="Manamperi, Aresha" uniqKey="Manamperi A" first="Aresha" last="Manamperi">Aresha Manamperi</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka</addr-line>
</nlm:aff>
<country xml:lang="fr">Sri Lanka</country>
<wicri:regionArea>Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama</wicri:regionArea>
<wicri:noRegion>Ragama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Gunawardena, Kithsiri" sort="Gunawardena, Kithsiri" uniqKey="Gunawardena K" first="Kithsiri" last="Gunawardena">Kithsiri Gunawardena</name>
<affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Department of Parasitology, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka</addr-line>
</nlm:aff>
<country xml:lang="fr">Sri Lanka</country>
<wicri:regionArea>Department of Parasitology, Faculty of Medicine, University of Kelaniya, Ragama</wicri:regionArea>
<wicri:noRegion>Ragama</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Abeyewickrama, Wimaladharma" sort="Abeyewickrama, Wimaladharma" uniqKey="Abeyewickrama W" first="Wimaladharma" last="Abeyewickrama">Wimaladharma Abeyewickrama</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka</addr-line>
</nlm:aff>
<country xml:lang="fr">Sri Lanka</country>
<wicri:regionArea>Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama</wicri:regionArea>
<wicri:noRegion>Ragama</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Department of Parasitology, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka</addr-line>
</nlm:aff>
<country xml:lang="fr">Sri Lanka</country>
<wicri:regionArea>Department of Parasitology, Faculty of Medicine, University of Kelaniya, Ragama</wicri:regionArea>
<wicri:noRegion>Ragama</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Asian Pacific Journal of Tropical Biomedicine</title>
<idno type="ISSN">2221-1691</idno>
<imprint><date when="2013">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Culicidae (parasitology)</term>
<term>Elephantiasis, Filarial (epidemiology)</term>
<term>Elephantiasis, Filarial (transmission)</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Humans</term>
<term>Polymerase Chain Reaction</term>
<term>Population Surveillance</term>
<term>Prevalence</term>
<term>Sri Lanka (epidemiology)</term>
<term>Wuchereria bancrofti (genetics)</term>
<term>Wuchereria bancrofti (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Culicidae (parasitologie)</term>
<term>Filariose lymphatique (transmission)</term>
<term>Filariose lymphatique (épidémiologie)</term>
<term>Humains</term>
<term>Prévalence</term>
<term>Réaction de polymérisation en chaîne</term>
<term>Sri Lanka (épidémiologie)</term>
<term>Surveillance de la population</term>
<term>Test ELISA</term>
<term>Wuchereria bancrofti (génétique)</term>
<term>Wuchereria bancrofti (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Sri Lanka</term>
</keywords>
<keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Elephantiasis, Filarial</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Wuchereria bancrofti</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Wuchereria bancrofti</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Wuchereria bancrofti</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Wuchereria bancrofti</term>
</keywords>
<keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Culicidae</term>
</keywords>
<keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Culicidae</term>
</keywords>
<keywords scheme="MESH" qualifier="transmission" xml:lang="en"><term>Elephantiasis, Filarial</term>
</keywords>
<keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Filariose lymphatique</term>
<term>Sri Lanka</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Humans</term>
<term>Polymerase Chain Reaction</term>
<term>Population Surveillance</term>
<term>Prevalence</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Humains</term>
<term>Prévalence</term>
<term>Réaction de polymérisation en chaîne</term>
<term>Surveillance de la population</term>
<term>Test ELISA</term>
</keywords>
<keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Sri Lanka</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><sec><title>Objective</title>
<p>To compare <italic>Wuchereria bancrofti</italic>
 (<italic>W. bancrofti</italic>
) infection rates of <italic>Culex quinquefasciatus</italic>
, using dissection and PCR-ELISA in two consecutive time periods (from 2007 to 2008 and from 2008 to 2009).</p>
</sec>
<sec><title>Methods</title>
<p>Mosquitoes were collected in 30 sentinel and 15 non-sentinel sites in 15 Medical Officer of Health areas of Gampaha District known for the presence of <italic>W. bancrofti</italic>
 transmission in two consecutive time period of 2007 to 2008 and 2008 to 2009. Captured mosquitoes were dissected to determine the <italic>W. bancrofti</italic>
 larvae (L<sub>1</sub>
, L<sub>2</sub>
, L<sub>3</sub>
). PCR was carried out using DNA extracted from mosquito pools (15 body parts/pool) utilizing the primers specific for Wb-SspI repeat. PCR products were analyzed by hybridization ELISA using fluorescein-labeled wild type specific probes. The prevalence of infected/infective mosquitoes in PCR pools (3 pools/site) was estimated using the PoolScreen™ algorithm and a novel probability-based method.</p>
</sec>
<sec><title>Results</title>
<p>Of 45 batches of mosquitoes dissected, <italic>W. bancrofti</italic>
 infected mosquitoes were found in 19 and 13 batches, with an infection rate of 13.29% and 3.10% with mean larval density of 8.7 and 1.0 larvae per mosquito for two study periods in the Gampaha District. Total of 405 pools of head, thorax and abdomen were processed by PCR-ELISA for each year. Of these, 51 and 31 pools were positive for <italic>W. bancrofti</italic>
 in the two study periods respectively. The association of dissection based prevalence rates with PCR based rates as determined by the Pearson correlation coefficient were 0.176 and 0.890 respectively for the two periods.</p>
</sec>
<sec><title>Conclusions</title>
<p>Data indicate that PCR-ELISA is more sensitive than the traditional dissection techniques for monitoring transmission intensity.</p>
</sec>
</div>
</front>
</TEI>
<affiliations><list><country><li>Singapour</li>
<li>Sri Lanka</li>
</country>
</list>
<tree><country name="Sri Lanka"><noRegion><name sortKey="Wijegunawardana, Asha Dilrukshi" sort="Wijegunawardana, Asha Dilrukshi" uniqKey="Wijegunawardana A" first="Asha Dilrukshi" last="Wijegunawardana">Asha Dilrukshi Wijegunawardana</name>
</noRegion>
<name sortKey="Abeyewickrama, Wimaladharma" sort="Abeyewickrama, Wimaladharma" uniqKey="Abeyewickrama W" first="Wimaladharma" last="Abeyewickrama">Wimaladharma Abeyewickrama</name>
<name sortKey="Abeyewickrama, Wimaladharma" sort="Abeyewickrama, Wimaladharma" uniqKey="Abeyewickrama W" first="Wimaladharma" last="Abeyewickrama">Wimaladharma Abeyewickrama</name>
<name sortKey="Gunawardane, Nilmini Silva" sort="Gunawardane, Nilmini Silva" uniqKey="Gunawardane N" first="Nilmini Silva" last="Gunawardane">Nilmini Silva Gunawardane</name>
<name sortKey="Gunawardena, Kithsiri" sort="Gunawardena, Kithsiri" uniqKey="Gunawardena K" first="Kithsiri" last="Gunawardena">Kithsiri Gunawardena</name>
<name sortKey="Manamperi, Aresha" sort="Manamperi, Aresha" uniqKey="Manamperi A" first="Aresha" last="Manamperi">Aresha Manamperi</name>
</country>
<country name="Singapour"><noRegion><name sortKey="Hapuarachchi, Chanditha" sort="Hapuarachchi, Chanditha" uniqKey="Hapuarachchi C" first="Chanditha" last="Hapuarachchi">Chanditha Hapuarachchi</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003661 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003661 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Sante
   |area=    LymphedemaV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     PMC:3642448
   |texte=   Evaluation of PCR-ELISA as a tool for monitoring transmission of Wuchereria bancrofti in District of Gampaha, Sri Lanka
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:23646302" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a LymphedemaV1

This area was generated with Dilib version V0.6.31.
Data generation: Sat Nov 4 17:40:35 2017. Site generation: Tue Feb 13 16:42:16 2024

Serveur d'exploration sur le lymphœdème

Evaluation of PCR-ELISA as a tool for monitoring transmission of Wuchereria bancrofti in District of Gampaha, Sri Lanka

Evaluation of PCR-ELISA as a tool for monitoring transmission of Wuchereria bancrofti in District of Gampaha, Sri Lanka

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur le lymphœdème
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.