Evaluation of PCR-ELISA as a tool for monitoring transmission of Wuchereria bancrofti in District of Gampaha, Sri Lanka
Identifieur interne : 003661 ( Main/Exploration ); précédent : 003660; suivant : 003662Evaluation of PCR-ELISA as a tool for monitoring transmission of Wuchereria bancrofti in District of Gampaha, Sri Lanka
Auteurs : Asha Dilrukshi Wijegunawardana [Sri Lanka] ; Nilmini Silva Gunawardane [Sri Lanka] ; Chanditha Hapuarachchi [Singapour] ; Aresha Manamperi [Sri Lanka] ; Kithsiri Gunawardena [Sri Lanka] ; Wimaladharma Abeyewickrama [Sri Lanka]Source :
- Asian Pacific Journal of Tropical Biomedicine [ 2221-1691 ] ; 2013.
Descripteurs français
- KwdFr :
- Animaux, Culicidae (parasitologie), Filariose lymphatique (transmission), Filariose lymphatique (épidémiologie), Humains, Prévalence, Réaction de polymérisation en chaîne, Sri Lanka (épidémiologie), Surveillance de la population, Test ELISA, Wuchereria bancrofti (génétique), Wuchereria bancrofti (immunologie).
- MESH :
- génétique : Wuchereria bancrofti.
- immunologie : Wuchereria bancrofti.
- parasitologie : Culicidae.
- épidémiologie : Filariose lymphatique, Sri Lanka.
- Animaux, Humains, Prévalence, Réaction de polymérisation en chaîne, Surveillance de la population, Test ELISA.
- Wicri :
- geographic : Sri Lanka.
English descriptors
- KwdEn :
- Animals, Culicidae (parasitology), Elephantiasis, Filarial (epidemiology), Elephantiasis, Filarial (transmission), Enzyme-Linked Immunosorbent Assay, Humans, Polymerase Chain Reaction, Population Surveillance, Prevalence, Sri Lanka (epidemiology), Wuchereria bancrofti (genetics), Wuchereria bancrofti (immunology).
- MESH :
- geographic , epidemiology : Sri Lanka.
- epidemiology : Elephantiasis, Filarial.
- genetics : Wuchereria bancrofti.
- immunology : Wuchereria bancrofti.
- parasitology : Culicidae.
- transmission : Elephantiasis, Filarial.
- Animals, Enzyme-Linked Immunosorbent Assay, Humans, Polymerase Chain Reaction, Population Surveillance, Prevalence.
Abstract
To compare
Mosquitoes were collected in 30 sentinel and 15 non-sentinel sites in 15 Medical Officer of Health areas of Gampaha District known for the presence of
Of 45 batches of mosquitoes dissected,
Data indicate that PCR-ELISA is more sensitive than the traditional dissection techniques for monitoring transmission intensity.
Url:
DOI: 10.1016/S2221-1691(13)60081-7
PubMed: 23646302
PubMed Central: 3642448
Affiliations:
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<author><name sortKey="Wijegunawardana, Asha Dilrukshi" sort="Wijegunawardana, Asha Dilrukshi" uniqKey="Wijegunawardana A" first="Asha Dilrukshi" last="Wijegunawardana">Asha Dilrukshi Wijegunawardana</name>
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<author><name sortKey="Gunawardena, Kithsiri" sort="Gunawardena, Kithsiri" uniqKey="Gunawardena K" first="Kithsiri" last="Gunawardena">Kithsiri Gunawardena</name>
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<author><name sortKey="Abeyewickrama, Wimaladharma" sort="Abeyewickrama, Wimaladharma" uniqKey="Abeyewickrama W" first="Wimaladharma" last="Abeyewickrama">Wimaladharma Abeyewickrama</name>
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<series><title level="j">Asian Pacific Journal of Tropical Biomedicine</title>
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<term>Culicidae (parasitology)</term>
<term>Elephantiasis, Filarial (epidemiology)</term>
<term>Elephantiasis, Filarial (transmission)</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Humans</term>
<term>Polymerase Chain Reaction</term>
<term>Population Surveillance</term>
<term>Prevalence</term>
<term>Sri Lanka (epidemiology)</term>
<term>Wuchereria bancrofti (genetics)</term>
<term>Wuchereria bancrofti (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Culicidae (parasitologie)</term>
<term>Filariose lymphatique (transmission)</term>
<term>Filariose lymphatique (épidémiologie)</term>
<term>Humains</term>
<term>Prévalence</term>
<term>Réaction de polymérisation en chaîne</term>
<term>Sri Lanka (épidémiologie)</term>
<term>Surveillance de la population</term>
<term>Test ELISA</term>
<term>Wuchereria bancrofti (génétique)</term>
<term>Wuchereria bancrofti (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Sri Lanka</term>
</keywords>
<keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Elephantiasis, Filarial</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Wuchereria bancrofti</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Wuchereria bancrofti</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Wuchereria bancrofti</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Wuchereria bancrofti</term>
</keywords>
<keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Culicidae</term>
</keywords>
<keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Culicidae</term>
</keywords>
<keywords scheme="MESH" qualifier="transmission" xml:lang="en"><term>Elephantiasis, Filarial</term>
</keywords>
<keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Filariose lymphatique</term>
<term>Sri Lanka</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Humans</term>
<term>Polymerase Chain Reaction</term>
<term>Population Surveillance</term>
<term>Prevalence</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Humains</term>
<term>Prévalence</term>
<term>Réaction de polymérisation en chaîne</term>
<term>Surveillance de la population</term>
<term>Test ELISA</term>
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<front><div type="abstract" xml:lang="en"><sec><title>Objective</title>
<p>To compare <italic>Wuchereria bancrofti</italic>
(<italic>W. bancrofti</italic>
) infection rates of <italic>Culex quinquefasciatus</italic>
, using dissection and PCR-ELISA in two consecutive time periods (from 2007 to 2008 and from 2008 to 2009).</p>
</sec>
<sec><title>Methods</title>
<p>Mosquitoes were collected in 30 sentinel and 15 non-sentinel sites in 15 Medical Officer of Health areas of Gampaha District known for the presence of <italic>W. bancrofti</italic>
transmission in two consecutive time period of 2007 to 2008 and 2008 to 2009. Captured mosquitoes were dissected to determine the <italic>W. bancrofti</italic>
larvae (L<sub>1</sub>
, L<sub>2</sub>
, L<sub>3</sub>
). PCR was carried out using DNA extracted from mosquito pools (15 body parts/pool) utilizing the primers specific for Wb-SspI repeat. PCR products were analyzed by hybridization ELISA using fluorescein-labeled wild type specific probes. The prevalence of infected/infective mosquitoes in PCR pools (3 pools/site) was estimated using the PoolScreen™ algorithm and a novel probability-based method.</p>
</sec>
<sec><title>Results</title>
<p>Of 45 batches of mosquitoes dissected, <italic>W. bancrofti</italic>
infected mosquitoes were found in 19 and 13 batches, with an infection rate of 13.29% and 3.10% with mean larval density of 8.7 and 1.0 larvae per mosquito for two study periods in the Gampaha District. Total of 405 pools of head, thorax and abdomen were processed by PCR-ELISA for each year. Of these, 51 and 31 pools were positive for <italic>W. bancrofti</italic>
in the two study periods respectively. The association of dissection based prevalence rates with PCR based rates as determined by the Pearson correlation coefficient were 0.176 and 0.890 respectively for the two periods.</p>
</sec>
<sec><title>Conclusions</title>
<p>Data indicate that PCR-ELISA is more sensitive than the traditional dissection techniques for monitoring transmission intensity.</p>
</sec>
</div>
</front>
</TEI>
<affiliations><list><country><li>Singapour</li>
<li>Sri Lanka</li>
</country>
</list>
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<name sortKey="Abeyewickrama, Wimaladharma" sort="Abeyewickrama, Wimaladharma" uniqKey="Abeyewickrama W" first="Wimaladharma" last="Abeyewickrama">Wimaladharma Abeyewickrama</name>
<name sortKey="Gunawardane, Nilmini Silva" sort="Gunawardane, Nilmini Silva" uniqKey="Gunawardane N" first="Nilmini Silva" last="Gunawardane">Nilmini Silva Gunawardane</name>
<name sortKey="Gunawardena, Kithsiri" sort="Gunawardena, Kithsiri" uniqKey="Gunawardena K" first="Kithsiri" last="Gunawardena">Kithsiri Gunawardena</name>
<name sortKey="Manamperi, Aresha" sort="Manamperi, Aresha" uniqKey="Manamperi A" first="Aresha" last="Manamperi">Aresha Manamperi</name>
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