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Quantitative estimation of hesperidin by HPTLC in different varieties of citrus peels

Identifieur interne : 002615 ( Main/Exploration ); précédent : 002614; suivant : 002616

Quantitative estimation of hesperidin by HPTLC in different varieties of citrus peels

Auteurs : Prawez Alam ; Aftab Alam ; Md. Khalid Anwer ; Saleh I. Alqasoumi

Source :

Asian Pacific Journal of Tropical Biomedicine [ 2221-1691 ] ; 2014.

RBID : PMC:3929787

Abstract

Objective

To develop a simple, selective, sensitive and accurate high-performance thin layer chromatography (HPTLC) method to determine the quantity of hesperidin in different varieties of citrus fruits.

Methods

The method was carried out in aluminum-backed silica gel 60 F₂₅₄ plates with ethyl acetate-methanol-water 15:3:2 (%, v/v) as mobile phase.

Results

A compact band was obtained for hesperidin at R_f value of (0.40±0.04). The calibration plot was linear in the range of 100-800 ng/spot of hesperidin and the correlation coefficient of 0.998 6 was indicative of good linear dependence of peak area on concentration. Limit of detection (8.87 ng/spot), limit of quantification (23.21 ng/spot), accuracy (less than 2%) and recovery (ranging from 98.55-99.38) were found satisfactory.

Conclusions

The method developed can be used for routine analysis of hesperidin in crude drug as well as in herbal and pharmaceutical dosage form containing citrus fruits as an ingredient.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3929787

DOI: 10.12980/APJTB.4.2014C1007
PubMed: 25182548
PubMed Central: 3929787

Affiliations:

Le document en format XML

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<front><div type="abstract" xml:lang="en"><sec><title>Objective</title>
<p>To develop a simple, selective, sensitive and accurate high-performance thin layer chromatography (HPTLC) method to determine the quantity of hesperidin in different varieties of citrus fruits.</p>
</sec>
<sec><title>Methods</title>
<p>The method was carried out in aluminum-backed silica gel 60 F<sub>254</sub>
 plates with ethyl acetate-methanol-water 15:3:2 (%, v/v) as mobile phase.</p>
</sec>
<sec><title>Results</title>
<p>A compact band was obtained for hesperidin at <italic>R<sub>f</sub>
</italic>
 value of (0.40±0.04). The calibration plot was linear in the range of 100-800 ng/spot of hesperidin and the correlation coefficient of 0.998 6 was indicative of good linear dependence of peak area on concentration. Limit of detection (8.87 ng/spot), limit of quantification (23.21 ng/spot), accuracy (less than 2%) and recovery (ranging from 98.55-99.38) were found satisfactory.</p>
</sec>
<sec><title>Conclusions</title>
<p>The method developed can be used for routine analysis of hesperidin in crude drug as well as in herbal and pharmaceutical dosage form containing citrus fruits as an ingredient.</p>
</sec>
</div>
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Quantitative estimation of hesperidin by HPTLC in different varieties of citrus peels

Quantitative estimation of hesperidin by HPTLC in different varieties of citrus peels

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Abstract

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