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Quantitative estimation of hesperidin by HPTLC in different varieties of citrus peels

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Quantitative estimation of hesperidin by HPTLC in different varieties of citrus peels

Auteurs : Prawez Alam ; Aftab Alam ; Md. Khalid Anwer ; Saleh I. Alqasoumi

Source :

Asian Pacific Journal of Tropical Biomedicine [ 2221-1691 ] ; 2014.

RBID : PMC:3929787

Abstract

Objective

To develop a simple, selective, sensitive and accurate high-performance thin layer chromatography (HPTLC) method to determine the quantity of hesperidin in different varieties of citrus fruits.

Methods

The method was carried out in aluminum-backed silica gel 60 F₂₅₄ plates with ethyl acetate-methanol-water 15:3:2 (%, v/v) as mobile phase.

Results

A compact band was obtained for hesperidin at R_f value of (0.40±0.04). The calibration plot was linear in the range of 100-800 ng/spot of hesperidin and the correlation coefficient of 0.998 6 was indicative of good linear dependence of peak area on concentration. Limit of detection (8.87 ng/spot), limit of quantification (23.21 ng/spot), accuracy (less than 2%) and recovery (ranging from 98.55-99.38) were found satisfactory.

Conclusions

The method developed can be used for routine analysis of hesperidin in crude drug as well as in herbal and pharmaceutical dosage form containing citrus fruits as an ingredient.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3929787

DOI: 10.12980/APJTB.4.2014C1007
PubMed: 25182548
PubMed Central: 3929787

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Prawez Alam

<affiliation><nlm:aff id="aff1"><addr-line>Department of Pharmacognosy, College of Pharmacy, Salman Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Kingdom of Saudi Arabia</addr-line>
</nlm:aff>
<wicri:noCountry code="subfield">Kingdom of Saudi Arabia</wicri:noCountry>
</affiliation>

Aftab Alam

<affiliation><nlm:aff id="aff1"><addr-line>Department of Pharmacognosy, College of Pharmacy, Salman Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Kingdom of Saudi Arabia</addr-line>
</nlm:aff>
<wicri:noCountry code="subfield">Kingdom of Saudi Arabia</wicri:noCountry>
</affiliation>

Md. Khalid Anwer

<affiliation><nlm:aff id="aff2"><addr-line>Department of Pharmaceutics, College of Pharmacy, Salman Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Kingdom of Saudi Arabia</addr-line>
</nlm:aff>
<wicri:noCountry code="subfield">Kingdom of Saudi Arabia</wicri:noCountry>
</affiliation>

Saleh I. Alqasoumi

<affiliation><nlm:aff id="aff3"><addr-line>Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Kingdom of Saudi Arabia</addr-line>
</nlm:aff>
<wicri:noCountry code="subfield">Kingdom of Saudi Arabia</wicri:noCountry>
</affiliation>

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<affiliation><nlm:aff id="aff1"><addr-line>Department of Pharmacognosy, College of Pharmacy, Salman Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Kingdom of Saudi Arabia</addr-line>
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<wicri:noCountry code="subfield">Kingdom of Saudi Arabia</wicri:noCountry>
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<author><name sortKey="Alam, Aftab" sort="Alam, Aftab" uniqKey="Alam A" first="Aftab" last="Alam">Aftab Alam</name>
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<author><name sortKey="Alqasoumi, Saleh I" sort="Alqasoumi, Saleh I" uniqKey="Alqasoumi S" first="Saleh I" last="Alqasoumi">Saleh I. Alqasoumi</name>
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<author><name sortKey="Alam, Prawez" sort="Alam, Prawez" uniqKey="Alam P" first="Prawez" last="Alam">Prawez Alam</name>
<affiliation><nlm:aff id="aff1"><addr-line>Department of Pharmacognosy, College of Pharmacy, Salman Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Kingdom of Saudi Arabia</addr-line>
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<wicri:noCountry code="subfield">Kingdom of Saudi Arabia</wicri:noCountry>
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<author><name sortKey="Alam, Aftab" sort="Alam, Aftab" uniqKey="Alam A" first="Aftab" last="Alam">Aftab Alam</name>
<affiliation><nlm:aff id="aff1"><addr-line>Department of Pharmacognosy, College of Pharmacy, Salman Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Kingdom of Saudi Arabia</addr-line>
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<wicri:noCountry code="subfield">Kingdom of Saudi Arabia</wicri:noCountry>
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<author><name sortKey="Anwer, Md Khalid" sort="Anwer, Md Khalid" uniqKey="Anwer M" first="Md. Khalid" last="Anwer">Md. Khalid Anwer</name>
<affiliation><nlm:aff id="aff2"><addr-line>Department of Pharmaceutics, College of Pharmacy, Salman Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Kingdom of Saudi Arabia</addr-line>
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<wicri:noCountry code="subfield">Kingdom of Saudi Arabia</wicri:noCountry>
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<author><name sortKey="Alqasoumi, Saleh I" sort="Alqasoumi, Saleh I" uniqKey="Alqasoumi S" first="Saleh I" last="Alqasoumi">Saleh I. Alqasoumi</name>
<affiliation><nlm:aff id="aff3"><addr-line>Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Kingdom of Saudi Arabia</addr-line>
</nlm:aff>
<wicri:noCountry code="subfield">Kingdom of Saudi Arabia</wicri:noCountry>
</affiliation>
</author>
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<series><title level="j">Asian Pacific Journal of Tropical Biomedicine</title>
<idno type="ISSN">2221-1691</idno>
<imprint><date when="2014">2014</date>
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<front><div type="abstract" xml:lang="en"><sec><title>Objective</title>
<p>To develop a simple, selective, sensitive and accurate high-performance thin layer chromatography (HPTLC) method to determine the quantity of hesperidin in different varieties of citrus fruits.</p>
</sec>
<sec><title>Methods</title>
<p>The method was carried out in aluminum-backed silica gel 60 F<sub>254</sub>
 plates with ethyl acetate-methanol-water 15:3:2 (%, v/v) as mobile phase.</p>
</sec>
<sec><title>Results</title>
<p>A compact band was obtained for hesperidin at <italic>R<sub>f</sub>
</italic>
 value of (0.40±0.04). The calibration plot was linear in the range of 100-800 ng/spot of hesperidin and the correlation coefficient of 0.998 6 was indicative of good linear dependence of peak area on concentration. Limit of detection (8.87 ng/spot), limit of quantification (23.21 ng/spot), accuracy (less than 2%) and recovery (ranging from 98.55-99.38) were found satisfactory.</p>
</sec>
<sec><title>Conclusions</title>
<p>The method developed can be used for routine analysis of hesperidin in crude drug as well as in herbal and pharmaceutical dosage form containing citrus fruits as an ingredient.</p>
</sec>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
  <front><journal-meta><journal-id journal-id-type="nlm-ta">Asian Pac J Trop Biomed</journal-id>
<journal-id journal-id-type="iso-abbrev">Asian Pac J Trop Biomed</journal-id>
<journal-id journal-id-type="publisher-id">APJTB</journal-id>
<journal-title-group><journal-title>Asian Pacific Journal of Tropical Biomedicine</journal-title>
</journal-title-group>
<issn pub-type="ppub">2221-1691</issn>
<publisher><publisher-name>Asian Pacific Tropical Medicine Press</publisher-name>
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</journal-meta>
<article-meta><article-id pub-id-type="pmid">25182548</article-id>
<article-id pub-id-type="pmc">3929787</article-id>
<article-id pub-id-type="publisher-id">apjtb-04-04-262</article-id>
<article-id pub-id-type="doi">10.12980/APJTB.4.2014C1007</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Basic Researches</subject>
</subj-group>
</article-categories>
<title-group><article-title>Quantitative estimation of hesperidin by HPTLC in different varieties of citrus peels</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Alam</surname>
<given-names>Prawez</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
<xref ref-type="corresp" rid="cor1">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Alam</surname>
<given-names>Aftab</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Anwer</surname>
<given-names>Md. Khalid</given-names>
</name>
<xref ref-type="aff" rid="aff2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Alqasoumi</surname>
<given-names>Saleh I</given-names>
</name>
<xref ref-type="aff" rid="aff3">3</xref>
</contrib>
</contrib-group>
<aff id="aff1"><label>1</label>
<addr-line>Department of Pharmacognosy, College of Pharmacy, Salman Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Kingdom of Saudi Arabia</addr-line>
</aff>
<aff id="aff2"><label>2</label>
<addr-line>Department of Pharmaceutics, College of Pharmacy, Salman Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Kingdom of Saudi Arabia</addr-line>
</aff>
<aff id="aff3"><label>3</label>
<addr-line>Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Kingdom of Saudi Arabia</addr-line>
</aff>
<contrib-group><contrib contrib-type="reviewer"><name><surname>Abdel-Kader</surname>
<given-names>Maged S</given-names>
<prefix>Prof.</prefix>
</name>
</contrib>
</contrib-group>
<aff id="aff4"><addr-line>Department of Pharmacognosy, College of Pharmacy, Alexandria University, Alexandria 21215, Egypt.</addr-line>
 E-mail:<email>mpharm101@hotmail.com</email>
</aff>
<author-notes><fn fn-type="other"><p><bold>Comments:</bold>
 This is a good study in which the authors work to estimate the amount of hesperidin in different varieties of citrus peels. Statically data proved that proposed method can be used in wide range for detection and estimation of hesperidin in different varieties of citrus peels. Details on Page 266</p>
</fn>
<corresp id="cor1">*Corresponding author: Dr. Prawez Alam, Department of Pharmacognosy, College of Pharmacy, Salman Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia. Tel: <phone>+966-532771723</phone>
 E-mail: <email>prawez_pharma@yahoo.com</email>
</corresp>
</author-notes>
<pub-date pub-type="ppub"><month>4</month>
<year>2014</year>
</pub-date>
<volume>4</volume>
<issue>4</issue>
<fpage>262</fpage>
<lpage>266</lpage>
<history><date date-type="received"><day>11</day>
<month>1</month>
<year>2013</year>
</date>
<date date-type="accepted"><day>22</day>
<month>2</month>
<year>2014</year>
</date>
</history>
<permissions><copyright-statement>© 2014 by the Asian Pacific Journal of Tropical Biomedicine. All rights reserved.</copyright-statement>
<copyright-year>2014</copyright-year>
</permissions>
<abstract><sec><title>Objective</title>
<p>To develop a simple, selective, sensitive and accurate high-performance thin layer chromatography (HPTLC) method to determine the quantity of hesperidin in different varieties of citrus fruits.</p>
</sec>
<sec><title>Methods</title>
<p>The method was carried out in aluminum-backed silica gel 60 F<sub>254</sub>
 plates with ethyl acetate-methanol-water 15:3:2 (%, v/v) as mobile phase.</p>
</sec>
<sec><title>Results</title>
<p>A compact band was obtained for hesperidin at <italic>R<sub>f</sub>
</italic>
 value of (0.40±0.04). The calibration plot was linear in the range of 100-800 ng/spot of hesperidin and the correlation coefficient of 0.998 6 was indicative of good linear dependence of peak area on concentration. Limit of detection (8.87 ng/spot), limit of quantification (23.21 ng/spot), accuracy (less than 2%) and recovery (ranging from 98.55-99.38) were found satisfactory.</p>
</sec>
<sec><title>Conclusions</title>
<p>The method developed can be used for routine analysis of hesperidin in crude drug as well as in herbal and pharmaceutical dosage form containing citrus fruits as an ingredient.</p>
</sec>
</abstract>
<kwd-group><kwd>HPTLC</kwd>
<kwd>Citrus</kwd>
<kwd>Hesperidin</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
</record>

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Quantitative estimation of hesperidin by HPTLC in different varieties of citrus peels

Quantitative estimation of hesperidin by HPTLC in different varieties of citrus peels

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Abstract

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