External Volume Expansion Modulates Vascular Growth and Functional Maturation in a Swine Model
Identifieur interne : 001074 ( Main/Exploration ); précédent : 001073; suivant : 001075External Volume Expansion Modulates Vascular Growth and Functional Maturation in a Swine Model
Auteurs : Huang-Kai Kao [Taïwan] ; Hsiang-Hao Hsu [Taïwan] ; Wen-Yu Chuang [Taïwan] ; Sheng-Chih Chen [Taïwan] ; Bin Chen [États-Unis] ; Shinn-Chih Wu [Taïwan] ; Lifei Guo [États-Unis]Source :
- Scientific Reports [ 2045-2322 ] ; 2016.
Abstract
Despite increasing application of the pre-grafting expansion during autologous fat transplantation in breast reconstruction, little is known about its mechanism of action. To address that, ventral skins of miniature pigs were treated over a 10-day or 21-day period, with continuous suction at −50 mm Hg via a 7-cm diameter rubber-lined suction-cup device. Soft tissue thickness increased immediately after this external volume expansion (EVE) treatment, such increase completely disappeared by the next day. In the dermis and subcutaneous fat, the EVE treated groups showed significant increases in blood vessel density evident by CD31 staining as well as in vascular networks layered with smooth muscle cells when compared with the control group. This finding was corroborated by the increased percentage of endothelial cells present in the treatment groups. There was no significant difference in the percentages of proliferating basal keratinocytes or adipocytes, nor in epidermal thickness. Moreover, the EVE had no effect on proliferation or differentiation potential of adipose stem cells. Taken together, the major effects of EVE appeared to be vascular remodeling and maturation of functional blood vessels. This understanding may help clinicians optimize the vascularity of the recipient bed to further improve fat graft survival.
Url:
DOI: 10.1038/srep25865
PubMed: 27174509
PubMed Central: 4865724
Affiliations:
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Tao-Yuan,<country>Taiwan</country></nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Hsu, Hsiang Hao" sort="Hsu, Hsiang Hao" uniqKey="Hsu H" first="Hsiang-Hao" last="Hsu">Hsiang-Hao Hsu</name><affiliation wicri:level="1"><nlm:aff id="a2"><institution>Kidney Research Center, Department of Nephrology, Division of Critical Care Nephrology, Chang Gung Memorial Hospital & Chang Gung University College of Medicine</institution>, Tao-Yuan,<country>Taiwan</country></nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Chuang, Wen Yu" sort="Chuang, Wen Yu" uniqKey="Chuang W" first="Wen-Yu" last="Chuang">Wen-Yu Chuang</name><affiliation wicri:level="1"><nlm:aff id="a3"><institution>Department of Pathology, Chang Gung Memorial Hospital & Chang Gung University College of Medicine</institution>, Tao-Yuan,<country>Taiwan</country></nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Sheng Chih" sort="Chen, Sheng Chih" uniqKey="Chen S" first="Sheng-Chih" last="Chen">Sheng-Chih Chen</name><affiliation wicri:level="1"><nlm:aff id="a4"><institution>Department of Animal Science, National Taiwan University</institution>, Taipei,<country>Taiwan</country></nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Bin" sort="Chen, Bin" uniqKey="Chen B" first="Bin" last="Chen">Bin Chen</name><affiliation wicri:level="1"><nlm:aff id="a5"><institution>Department of Plastic Surgery, Lahey Hospital & Medical Center</institution>, Burlington, MA,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Wu, Shinn Chih" sort="Wu, Shinn Chih" uniqKey="Wu S" first="Shinn-Chih" last="Wu">Shinn-Chih Wu</name><affiliation wicri:level="1"><nlm:aff id="a4"><institution>Department of Animal Science, National Taiwan University</institution>, Taipei,<country>Taiwan</country></nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Guo, Lifei" sort="Guo, Lifei" uniqKey="Guo L" first="Lifei" last="Guo">Lifei Guo</name><affiliation wicri:level="1"><nlm:aff id="a5"><institution>Department of Plastic Surgery, Lahey Hospital & Medical Center</institution>, Burlington, MA,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">Scientific Reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Despite increasing application of the pre-grafting expansion during autologous fat transplantation in breast reconstruction, little is known about its mechanism of action. To address that, ventral skins of miniature pigs were treated over a 10-day or 21-day period, with continuous suction at −50 mm Hg via a 7-cm diameter rubber-lined suction-cup device. Soft tissue thickness increased immediately after this external volume expansion (EVE) treatment, such increase completely disappeared by the next day. In the dermis and subcutaneous fat, the EVE treated groups showed significant increases in blood vessel density evident by CD31 staining as well as in vascular networks layered with smooth muscle cells when compared with the control group. This finding was corroborated by the increased percentage of endothelial cells present in the treatment groups. There was no significant difference in the percentages of proliferating basal keratinocytes or adipocytes, nor in epidermal thickness. Moreover, the EVE had no effect on proliferation or differentiation potential of adipose stem cells. Taken together, the major effects of EVE appeared to be vascular remodeling and maturation of functional blood vessels. This understanding may help clinicians optimize the vascularity of the recipient bed to further improve fat graft survival.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Khouri, R K" uniqKey="Khouri R">R. K. Khouri</name></author><author><name sortKey="Schlenz, I" uniqKey="Schlenz I">I. Schlenz</name></author><author><name sortKey="Murphy, B J" uniqKey="Murphy B">B. J. Murphy</name></author><author><name sortKey="Baker, D J" uniqKey="Baker D">D. J. Baker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Khouri, R K" uniqKey="Khouri R">R. K. Khouri</name></author><author><name sortKey="Rigotti, G" uniqKey="Rigotti G">G. Rigotti</name></author><author><name sortKey="Cardoso, E" uniqKey="Cardoso E">E. 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Kim</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Taïwan</li><li>États-Unis</li></country></list><tree><country name="Taïwan"><noRegion><name sortKey="Kao, Huang Kai" sort="Kao, Huang Kai" uniqKey="Kao H" first="Huang-Kai" last="Kao">Huang-Kai Kao</name></noRegion><name sortKey="Chen, Sheng Chih" sort="Chen, Sheng Chih" uniqKey="Chen S" first="Sheng-Chih" last="Chen">Sheng-Chih Chen</name><name sortKey="Chuang, Wen Yu" sort="Chuang, Wen Yu" uniqKey="Chuang W" first="Wen-Yu" last="Chuang">Wen-Yu Chuang</name><name sortKey="Hsu, Hsiang Hao" sort="Hsu, Hsiang Hao" uniqKey="Hsu H" first="Hsiang-Hao" last="Hsu">Hsiang-Hao Hsu</name><name sortKey="Wu, Shinn Chih" sort="Wu, Shinn Chih" uniqKey="Wu S" first="Shinn-Chih" last="Wu">Shinn-Chih Wu</name></country><country name="États-Unis"><noRegion><name sortKey="Chen, Bin" sort="Chen, Bin" uniqKey="Chen B" first="Bin" last="Chen">Bin Chen</name></noRegion><name sortKey="Guo, Lifei" sort="Guo, Lifei" uniqKey="Guo L" first="Lifei" last="Guo">Lifei Guo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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