Dual-channel in-situ optical imaging system for quantifying lipid uptake and lymphatic pump function
Identifieur interne : 004362 ( Main/Curation ); précédent : 004361; suivant : 004363Dual-channel in-situ optical imaging system for quantifying lipid uptake and lymphatic pump function
Auteurs : Timothy Kassis [Géorgie (pays)] ; Alison B. Kohan [États-Unis] ; Michael J. Weiler [Géorgie (pays)] ; Matthew E. Nipper [Géorgie (pays)] ; Rachel Cornelius [Géorgie (pays)] ; Patrick Tso [États-Unis] ; J. Brandon Dixon [Géorgie (pays)]Source :
- Journal of Biomedical Optics [ 1083-3668 ] ; 2012.
Abstract
Nearly all dietary lipids are transported from the intestine to venous circulation through the lymphatic system, yet the mechanisms that regulate this process remain unclear. Elucidating the mechanisms involved in the functional response of lymphatics to changes in lipid load would provide valuable insight into recent implications of lymphatic dysfunction in lipid related diseases. Therefore, we sought to develop an
Url:
DOI: 10.1117/1.JBO.17.8.086005
PubMed: 23224192
PubMed Central: 3413897
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PMC:3413897Le document en format XML
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optical imaging system for quantifying lipid uptake and lymphatic pump function</title>
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, Wallace H. Coulter Department of Biomedical Engineering, Atlanta, Georgia</nlm:aff>
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<author><name sortKey="Nipper, Matthew E" sort="Nipper, Matthew E" uniqKey="Nipper M" first="Matthew E." last="Nipper">Matthew E. Nipper</name>
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, Parker H. Petit Institute for Bioengineering and Bioscience, Atlanta, Georgia</nlm:aff>
<country xml:lang="fr">Géorgie (pays)</country>
<wicri:regionArea>, Parker H. Petit Institute for Bioengineering and Bioscience, Atlanta</wicri:regionArea>
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, George W. Woodruff School of Mechanical Engineering, Atlanta, Georgia</nlm:aff>
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, Wallace H. Coulter Department of Biomedical Engineering, Atlanta, Georgia</nlm:aff>
<country xml:lang="fr">Géorgie (pays)</country>
<wicri:regionArea>, Wallace H. Coulter Department of Biomedical Engineering, Atlanta</wicri:regionArea>
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, Department of Pathology and Laboratory Medicine, Cincinnati, Ohio</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Ohio</region>
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<wicri:cityArea>, Department of Pathology and Laboratory Medicine, Cincinnati</wicri:cityArea>
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<author><name sortKey="Brandon Dixon, J" sort="Brandon Dixon, J" uniqKey="Brandon Dixon J" first="J." last="Brandon Dixon">J. Brandon Dixon</name>
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, Parker H. Petit Institute for Bioengineering and Bioscience, Atlanta, Georgia</nlm:aff>
<country xml:lang="fr">Géorgie (pays)</country>
<wicri:regionArea>, Parker H. Petit Institute for Bioengineering and Bioscience, Atlanta</wicri:regionArea>
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, George W. Woodruff School of Mechanical Engineering, Atlanta, Georgia</nlm:aff>
<country xml:lang="fr">Géorgie (pays)</country>
<wicri:regionArea>, George W. Woodruff School of Mechanical Engineering, Atlanta</wicri:regionArea>
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, Wallace H. Coulter Department of Biomedical Engineering, Atlanta, Georgia</nlm:aff>
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<front><div type="abstract" xml:lang="en"><title>Abstract.</title>
<p>Nearly all dietary lipids are transported from the intestine to venous circulation through the lymphatic system, yet the mechanisms that regulate this process remain unclear. Elucidating the mechanisms involved in the functional response of lymphatics to changes in lipid load would provide valuable insight into recent implications of lymphatic dysfunction in lipid related diseases. Therefore, we sought to develop an <italic>in situ</italic>
imaging system to quantify and correlate lymphatic function as it relates to lipid transport. The imaging platform provides the capability of dual-channel imaging of both high-speed bright-field video and fluorescence simultaneously. Utilizing post-acquisition image processing algorithms, we can quantify correlations between vessel pump function, lymph flow, and lipid concentration of mesenteric lymphatic vessels <italic>in situ</italic>
. All image analysis is automated with customized LabVIEW virtual instruments; local flow is measured through lymphocyte velocity tracking, vessel contraction through measurements of the vessel wall displacement, and lipid uptake through fluorescence intensity tracking of an orally administered fluorescently labelled fatty acid analogue, BODIPY FL <inline-formula><mml:math id="M1"><mml:mrow><mml:msub><mml:mrow><mml:mi mathvariant="normal">C</mml:mi>
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. This system will prove to be an invaluable tool for scientists studying intestinal lymphatic function in health and disease, and those investigating strategies for targeting the lymphatics with orally delivered drugs to avoid first pass metabolism.</p>
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