H2N2V1, PubMed, Corpus, bibRecord, 000419

Rapid detection of influenza A neuraminidase subtypes by cDNA amplification coupled to a simple DNA enzyme immunoassay.

Identifieur interne : 000419 ( PubMed/Corpus ); précédent : 000418; suivant : 000420

Rapid detection of influenza A neuraminidase subtypes by cDNA amplification coupled to a simple DNA enzyme immunoassay.

Auteurs : B. Schweiger ; I. Lange ; R. Heckler ; H. Willers ; E. Schreier

Source :

Archives of virology [ 0304-8608 ] ; 1994.

RBID : pubmed:7832649

English descriptors

KwdEn :
- Base Sequence, DNA Probes, DNA, Complementary (analysis), DNA, Complementary (genetics), Genome, Viral, Immunoenzyme Techniques, Influenza A virus (classification), Influenza A virus (enzymology), Influenza A virus (genetics), Molecular Sequence Data, Neuraminidase (classification), Neuraminidase (genetics), Nucleic Acid Hybridization, Polymerase Chain Reaction, Sensitivity and Specificity.
MESH :
- chemical , analysis : DNA, Complementary.
- chemical , classification : Neuraminidase.
- chemical , genetics : DNA, Complementary, Neuraminidase.
- chemical : DNA Probes.
- classification : Influenza A virus.
- enzymology : Influenza A virus.
- genetics : Influenza A virus.
- Base Sequence, Genome, Viral, Immunoenzyme Techniques, Molecular Sequence Data, Nucleic Acid Hybridization, Polymerase Chain Reaction, Sensitivity and Specificity.

Abstract

A newly developed colorimetric method, DNA enzyme immunoassay (DEIA), was applied to the detection of neuraminidase subtypes N1 and N2 of influenza A viruses. Reverse transcription and polymerase chain reaction with universal primers were used for genomic amplification of H1N1, H2N2, and H3N2 strains. Following amplification, an aliquot of the PCR product was hybridized to biotinylated DNA sequences (N1/N2 probes) immobilized on microtiter wells. The hybridization event was revealed by monoclonal antibodies to double stranded DNA in a standard ELISA reaction. The assay described here was able to distinguish accurately between the two neuraminidase subtypes of human influenza A viruses. It is a simple and rapid method facilitating the handling of a large number of samples and therefore seems to be easily applicable to diagnostic laboratories.

DOI: 10.1007/bf01310805
PubMed: 7832649

Links to Exploration step

pubmed:7832649

Le document en format XML

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<author><name sortKey="Schweiger, B" sort="Schweiger, B" uniqKey="Schweiger B" first="B" last="Schweiger">B. Schweiger</name>
<affiliation><nlm:affiliation>Robert Koch-Institut, Berlin, Federal Republic of Germany.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Lange, I" sort="Lange, I" uniqKey="Lange I" first="I" last="Lange">I. Lange</name>
</author>
<author><name sortKey="Heckler, R" sort="Heckler, R" uniqKey="Heckler R" first="R" last="Heckler">R. Heckler</name>
</author>
<author><name sortKey="Willers, H" sort="Willers, H" uniqKey="Willers H" first="H" last="Willers">H. Willers</name>
</author>
<author><name sortKey="Schreier, E" sort="Schreier, E" uniqKey="Schreier E" first="E" last="Schreier">E. Schreier</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Rapid detection of influenza A neuraminidase subtypes by cDNA amplification coupled to a simple DNA enzyme immunoassay.</title>
<author><name sortKey="Schweiger, B" sort="Schweiger, B" uniqKey="Schweiger B" first="B" last="Schweiger">B. Schweiger</name>
<affiliation><nlm:affiliation>Robert Koch-Institut, Berlin, Federal Republic of Germany.</nlm:affiliation>
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<author><name sortKey="Lange, I" sort="Lange, I" uniqKey="Lange I" first="I" last="Lange">I. Lange</name>
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<author><name sortKey="Heckler, R" sort="Heckler, R" uniqKey="Heckler R" first="R" last="Heckler">R. Heckler</name>
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<author><name sortKey="Willers, H" sort="Willers, H" uniqKey="Willers H" first="H" last="Willers">H. Willers</name>
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<author><name sortKey="Schreier, E" sort="Schreier, E" uniqKey="Schreier E" first="E" last="Schreier">E. Schreier</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term>
<term>DNA Probes</term>
<term>DNA, Complementary (analysis)</term>
<term>DNA, Complementary (genetics)</term>
<term>Genome, Viral</term>
<term>Immunoenzyme Techniques</term>
<term>Influenza A virus (classification)</term>
<term>Influenza A virus (enzymology)</term>
<term>Influenza A virus (genetics)</term>
<term>Molecular Sequence Data</term>
<term>Neuraminidase (classification)</term>
<term>Neuraminidase (genetics)</term>
<term>Nucleic Acid Hybridization</term>
<term>Polymerase Chain Reaction</term>
<term>Sensitivity and Specificity</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Complementary</term>
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<keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term>
<term>Genome, Viral</term>
<term>Immunoenzyme Techniques</term>
<term>Molecular Sequence Data</term>
<term>Nucleic Acid Hybridization</term>
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<front><div type="abstract" xml:lang="en">A newly developed colorimetric method, DNA enzyme immunoassay (DEIA), was applied to the detection of neuraminidase subtypes N1 and N2 of influenza A viruses. Reverse transcription and polymerase chain reaction with universal primers were used for genomic amplification of H1N1, H2N2, and H3N2 strains. Following amplification, an aliquot of the PCR product was hybridized to biotinylated DNA sequences (N1/N2 probes) immobilized on microtiter wells. The hybridization event was revealed by monoclonal antibodies to double stranded DNA in a standard ELISA reaction. The assay described here was able to distinguish accurately between the two neuraminidase subtypes of human influenza A viruses. It is a simple and rapid method facilitating the handling of a large number of samples and therefore seems to be easily applicable to diagnostic laboratories.</div>
</front>
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<Abstract><AbstractText>A newly developed colorimetric method, DNA enzyme immunoassay (DEIA), was applied to the detection of neuraminidase subtypes N1 and N2 of influenza A viruses. Reverse transcription and polymerase chain reaction with universal primers were used for genomic amplification of H1N1, H2N2, and H3N2 strains. Following amplification, an aliquot of the PCR product was hybridized to biotinylated DNA sequences (N1/N2 probes) immobilized on microtiter wells. The hybridization event was revealed by monoclonal antibodies to double stranded DNA in a standard ELISA reaction. The assay described here was able to distinguish accurately between the two neuraminidase subtypes of human influenza A viruses. It is a simple and rapid method facilitating the handling of a large number of samples and therefore seems to be easily applicable to diagnostic laboratories.</AbstractText>
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Rapid detection of influenza A neuraminidase subtypes by cDNA amplification coupled to a simple DNA enzyme immunoassay.

Rapid detection of influenza A neuraminidase subtypes by cDNA amplification coupled to a simple DNA enzyme immunoassay.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

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