Visual detection of H3 subtype avian influenza viruses by reverse transcription loop-mediated isothermal amplification assay
Identifieur interne : 000C39 ( Pmc/Curation ); précédent : 000C38; suivant : 000C40Visual detection of H3 subtype avian influenza viruses by reverse transcription loop-mediated isothermal amplification assay
Auteurs : Yi Peng [République populaire de Chine] ; Zhixun Xie [République populaire de Chine] ; Jiabo Liu [République populaire de Chine] ; Yaoshan Pang [République populaire de Chine] ; Xianwen Deng [République populaire de Chine] ; Zhiqin Xie [République populaire de Chine] ; Liji Xie [République populaire de Chine] ; Qing Fan [République populaire de Chine] ; Jiaxun Feng [République populaire de Chine] ; Mazhar I. Khan [États-Unis]Source :
- Virology Journal [ 1743-422X ] ; 2011.
Abstract
Recent epidemiological investigation of different HA subtypes of avian influenza viruses (AIVs) shows that the H3 subtype is the most predominant among low pathogenic AIVs (LPAIVs), and the seasonal variations in isolation of H3 subtype AIVs are consistent with that of human H3 subtype influenza viruses. Consequently, the development of a rapid, simple, sensitive detection method for H3 subtype AIVs is required. The loop-mediated isothermal amplification (LAMP) assay is a simple, rapid, sensitive and cost-effective nucleic acid amplification method that does not require any specialized equipment.
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to detect the H3 subtype AIVs visually. Specific primer sets target the sequences of the hemagglutinin (HA) gene of H3 subtype AIVs were designed, and assay reaction conditions were optimized. The established assay was performed in a water bath for 50 minutes, and the amplification result was visualized directly as well as under ultraviolet (UV) light reflections. The detection limit of the RT-LAMP assay was 0.1pg total RNA of virus, which was one hundred-fold higher than that of RT-PCR. The results on specificity indicated that the assay had no cross-reactions with other subtype AIVs or avian respiratory pathogens. Furthermore, a total of 176 clinical samples collected from birds at the various live-bird markets (LBMs) were subjected to the H3-subtype-specific RT-LAMP (H3-RT-LAMP). Thirty-eight H3 subtype AIVs were identified from the 176 clinical samples that were consistent with that of virus isolation.
The newly developed H3-RT-LAMP assay is simple, sensitive, rapid and can identify H3 subtype AIVs visually. Consequently, it will be a very useful screening assay for the surveillance of H3 subtype AIVs in underequipped laboratories as well as in field conditions.
Url:
DOI: 10.1186/1743-422X-8-337
PubMed: 21729297
PubMed Central: 3154870
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530001, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biotechnology, Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi 530001</wicri:regionArea></affiliation></author><author><name sortKey="Deng, Xianwen" sort="Deng, Xianwen" uniqKey="Deng X" first="Xianwen" last="Deng">Xianwen Deng</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biotechnology, Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi 530001, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biotechnology, Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi 530001</wicri:regionArea></affiliation></author><author><name sortKey="Xie, Zhiqin" sort="Xie, Zhiqin" uniqKey="Xie Z" first="Zhiqin" last="Xie">Zhiqin Xie</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biotechnology, Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi 530001, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biotechnology, Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi 530001</wicri:regionArea></affiliation></author><author><name sortKey="Xie, Liji" sort="Xie, Liji" uniqKey="Xie L" first="Liji" last="Xie">Liji Xie</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biotechnology, Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi 530001, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biotechnology, Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi 530001</wicri:regionArea></affiliation></author><author><name sortKey="Fan, Qing" sort="Fan, Qing" uniqKey="Fan Q" first="Qing" last="Fan">Qing Fan</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biotechnology, Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi 530001, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biotechnology, Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi 530001</wicri:regionArea></affiliation></author><author><name sortKey="Feng, Jiaxun" sort="Feng, Jiaxun" uniqKey="Feng J" first="Jiaxun" last="Feng">Jiaxun Feng</name><affiliation wicri:level="1"><nlm:aff id="I3">Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004</wicri:regionArea></affiliation></author><author><name sortKey="Khan, Mazhar I" sort="Khan, Mazhar I" uniqKey="Khan M" first="Mazhar I" last="Khan">Mazhar I. Khan</name><affiliation wicri:level="1"><nlm:aff id="I2">Department of Pathobiology and Veterinary Science, University of Connecticut, 61 North Eagleville Road Storrs, CT 06269-3089, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathobiology and Veterinary Science, University of Connecticut, 61 North Eagleville Road Storrs, CT 06269-3089</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">21729297</idno><idno type="pmc">3154870</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3154870</idno><idno type="RBID">PMC:3154870</idno><idno type="doi">10.1186/1743-422X-8-337</idno><date when="2011">2011</date><idno type="wicri:Area/Pmc/Corpus">000C39</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000C39</idno><idno type="wicri:Area/Pmc/Curation">000C39</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000C39</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Visual detection of H3 subtype avian influenza viruses by reverse transcription loop-mediated isothermal amplification assay</title><author><name sortKey="Peng, Yi" sort="Peng, Yi" uniqKey="Peng Y" first="Yi" last="Peng">Yi Peng</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biotechnology, Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi 530001, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biotechnology, Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi 530001</wicri:regionArea></affiliation></author><author><name sortKey="Xie, Zhixun" sort="Xie, Zhixun" uniqKey="Xie Z" first="Zhixun" last="Xie">Zhixun Xie</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biotechnology, Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi 530001, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biotechnology, Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi 530001</wicri:regionArea></affiliation></author><author><name sortKey="Liu, Jiabo" sort="Liu, Jiabo" uniqKey="Liu J" first="Jiabo" last="Liu">Jiabo Liu</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biotechnology, Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi 530001, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biotechnology, Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi 530001</wicri:regionArea></affiliation></author><author><name sortKey="Pang, Yaoshan" sort="Pang, Yaoshan" uniqKey="Pang Y" first="Yaoshan" last="Pang">Yaoshan Pang</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biotechnology, Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi 530001, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biotechnology, Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi 530001</wicri:regionArea></affiliation></author><author><name sortKey="Deng, Xianwen" sort="Deng, Xianwen" uniqKey="Deng X" first="Xianwen" last="Deng">Xianwen Deng</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biotechnology, Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi 530001, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biotechnology, Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi 530001</wicri:regionArea></affiliation></author><author><name sortKey="Xie, Zhiqin" sort="Xie, Zhiqin" uniqKey="Xie Z" first="Zhiqin" last="Xie">Zhiqin Xie</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biotechnology, Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi 530001, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biotechnology, Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi 530001</wicri:regionArea></affiliation></author><author><name sortKey="Xie, Liji" sort="Xie, Liji" uniqKey="Xie L" first="Liji" last="Xie">Liji Xie</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biotechnology, Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi 530001, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biotechnology, Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi 530001</wicri:regionArea></affiliation></author><author><name sortKey="Fan, Qing" sort="Fan, Qing" uniqKey="Fan Q" first="Qing" last="Fan">Qing Fan</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biotechnology, Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi 530001, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Biotechnology, Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi 530001</wicri:regionArea></affiliation></author><author><name sortKey="Feng, Jiaxun" sort="Feng, Jiaxun" uniqKey="Feng J" first="Jiaxun" last="Feng">Jiaxun Feng</name><affiliation wicri:level="1"><nlm:aff id="I3">Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004</wicri:regionArea></affiliation></author><author><name sortKey="Khan, Mazhar I" sort="Khan, Mazhar I" uniqKey="Khan M" first="Mazhar I" last="Khan">Mazhar I. Khan</name><affiliation wicri:level="1"><nlm:aff id="I2">Department of Pathobiology and Veterinary Science, University of Connecticut, 61 North Eagleville Road Storrs, CT 06269-3089, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathobiology and Veterinary Science, University of Connecticut, 61 North Eagleville Road Storrs, CT 06269-3089</wicri:regionArea></affiliation></author></analytic><series><title level="j">Virology Journal</title><idno type="eISSN">1743-422X</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Recent epidemiological investigation of different HA subtypes of avian influenza viruses (AIVs) shows that the H3 subtype is the most predominant among low pathogenic AIVs (LPAIVs), and the seasonal variations in isolation of H3 subtype AIVs are consistent with that of human H3 subtype influenza viruses. Consequently, the development of a rapid, simple, sensitive detection method for H3 subtype AIVs is required. The loop-mediated isothermal amplification (LAMP) assay is a simple, rapid, sensitive and cost-effective nucleic acid amplification method that does not require any specialized equipment.</p></sec><sec><title>Results</title><p>A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to detect the H3 subtype AIVs visually. Specific primer sets target the sequences of the hemagglutinin (HA) gene of H3 subtype AIVs were designed, and assay reaction conditions were optimized. The established assay was performed in a water bath for 50 minutes, and the amplification result was visualized directly as well as under ultraviolet (UV) light reflections. The detection limit of the RT-LAMP assay was 0.1pg total RNA of virus, which was one hundred-fold higher than that of RT-PCR. The results on specificity indicated that the assay had no cross-reactions with other subtype AIVs or avian respiratory pathogens. Furthermore, a total of 176 clinical samples collected from birds at the various live-bird markets (LBMs) were subjected to the H3-subtype-specific RT-LAMP (H3-RT-LAMP). Thirty-eight H3 subtype AIVs were identified from the 176 clinical samples that were consistent with that of virus isolation.</p></sec><sec><title>Conclusions</title><p>The newly developed H3-RT-LAMP assay is simple, sensitive, rapid and can identify H3 subtype AIVs visually. Consequently, it will be a very useful screening assay for the surveillance of H3 subtype AIVs in underequipped laboratories as well as in field conditions.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Webster, Rg" uniqKey="Webster R">RG Webster</name></author><author><name sortKey="Bean, Wj" uniqKey="Bean W">WJ Bean</name></author><author><name sortKey="Gorman, Ot" uniqKey="Gorman O">OT Gorman</name></author><author><name sortKey="Chambers, Tm" uniqKey="Chambers T">TM Chambers</name></author><author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y Kawaoka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Horimoto, T" uniqKey="Horimoto T">T Horimoto</name></author><author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y Kawaoka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fouchier, Ram" uniqKey="Fouchier R">RAM Fouchier</name></author><author><name sortKey="Munster, V" uniqKey="Munster V">V Munster</name></author><author><name sortKey="Wallensten, A" uniqKey="Wallensten A">A Wallensten</name></author><author><name sortKey="Bestebroer, Tm" uniqKey="Bestebroer T">TM Bestebroer</name></author><author><name sortKey="Herfst, S" uniqKey="Herfst S">S Herfst</name></author><author><name sortKey="Smith, D" uniqKey="Smith D">D Smith</name></author><author><name sortKey="Rimmelzwaan, Gf" uniqKey="Rimmelzwaan G">GF Rimmelzwaan</name></author><author><name sortKey="Olsen, B" uniqKey="Olsen B">B Olsen</name></author><author><name sortKey="Osterhaus, Adme" uniqKey="Osterhaus A">ADME Osterhaus</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Alexander, Dj" uniqKey="Alexander D">DJ Alexander</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Edwards, S" uniqKey="Edwards S">S Edwards</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Scholtissek, C" uniqKey="Scholtissek C">C Scholtissek</name></author><author><name sortKey="Rohde, W" uniqKey="Rohde W">W Rohde</name></author><author><name sortKey="Von Hoyningen, V" uniqKey="Von Hoyningen V">V Von-Hoyningen</name></author><author><name sortKey="Rott, R" uniqKey="Rott R">R Rott</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Karasin, Ai" uniqKey="Karasin A">AI Karasin</name></author><author><name sortKey="Carman, S" uniqKey="Carman S">S Carman</name></author><author><name sortKey="Olsen, Cw" uniqKey="Olsen C">CW Olsen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peng, Y" uniqKey="Peng Y">Y Peng</name></author><author><name sortKey="Zhang, W" uniqKey="Zhang W">W Zhang</name></author><author><name sortKey="Xue, F" uniqKey="Xue F">F Xue</name></author><author><name sortKey="Wang, Wb" uniqKey="Wang W">WB Wang</name></author><author><name sortKey="Li, Yf" uniqKey="Li Y">YF Li</name></author><author><name sortKey="Meng, Cc" uniqKey="Meng C">CC Meng</name></author><author><name sortKey="Zhang, Wj" uniqKey="Zhang W">WJ Zhang</name></author><author><name sortKey="Liu, Xf" uniqKey="Liu X">XF Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Song, Ms" uniqKey="Song M">MS Song</name></author><author><name sortKey="Oh, Tk" uniqKey="Oh T">TK Oh</name></author><author><name sortKey="Moon, Hj" uniqKey="Moon H">HJ Moon</name></author><author><name sortKey="Yoo, Dw" uniqKey="Yoo D">DW Yoo</name></author><author><name sortKey="Lee, Eh" uniqKey="Lee E">EH Lee</name></author><author><name sortKey="Lee, Js" uniqKey="Lee J">JS Lee</name></author><author><name sortKey="Kim, Cj" uniqKey="Kim C">CJ Kim</name></author><author><name sortKey="Yoo, Gj" uniqKey="Yoo G">GJ Yoo</name></author><author><name sortKey="Kim, H" uniqKey="Kim H">H Kim</name></author><author><name sortKey="Choi, Yk" uniqKey="Choi Y">YK Choi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Campitelli, L" uniqKey="Campitelli L">L Campitelli</name></author><author><name sortKey="Fabiani, C" uniqKey="Fabiani C">C Fabiani</name></author><author><name sortKey="Puzelli, S" uniqKey="Puzelli S">S Puzelli</name></author><author><name sortKey="Fioretti, A" uniqKey="Fioretti A">A Fioretti</name></author><author><name sortKey="Foni, E" uniqKey="Foni E">E Foni</name></author><author><name sortKey="De Marco, Ma" uniqKey="De Marco M">MA De Marco</name></author><author><name sortKey="Krauss, S" uniqKey="Krauss S">S Krauss</name></author><author><name sortKey="Webster, Rg" uniqKey="Webster R">RG Webster</name></author><author><name sortKey="Donatelli, I" uniqKey="Donatelli I">I Donatelli</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Notomi, T" uniqKey="Notomi T">T Notomi</name></author><author><name sortKey="Okayama, H" uniqKey="Okayama H">H Okayama</name></author><author><name sortKey="Masubuchi, H" uniqKey="Masubuchi H">H Masubuchi</name></author><author><name sortKey="Yonekawa, T" uniqKey="Yonekawa T">T Yonekawa</name></author><author><name sortKey="Watanabe, K" uniqKey="Watanabe K">K Watanabe</name></author><author><name sortKey="Amino, N" uniqKey="Amino N">N Amino</name></author><author><name sortKey="Hase, T" uniqKey="Hase T">T Hase</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mori, Y" uniqKey="Mori Y">Y Mori</name></author><author><name sortKey="Nagamine, K" uniqKey="Nagamine K">K Nagamine</name></author><author><name sortKey="Tomita, N" uniqKey="Tomita N">N Tomita</name></author><author><name sortKey="Notomi, T" uniqKey="Notomi T">T Notomi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tomita, N" uniqKey="Tomita N">N Tomita</name></author><author><name sortKey="Mori, Y" uniqKey="Mori Y">Y Mori</name></author><author><name sortKey="Kanda, H" uniqKey="Kanda H">H Kanda</name></author><author><name sortKey="Notomi, T" uniqKey="Notomi T">T Notomi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rovira, A" uniqKey="Rovira A">A Rovira</name></author><author><name sortKey="Abrahante, J" uniqKey="Abrahante J">J Abrahante</name></author><author><name sortKey="Murtaugh, M" uniqKey="Murtaugh M">M Murtaugh</name></author><author><name sortKey="Mun Oz Zanzi, C" uniqKey="Mun Oz Zanzi C">C Mun˜ oz-Zanzi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pham, Hm" uniqKey="Pham H">HM Pham</name></author><author><name sortKey="Nakajima, C" uniqKey="Nakajima C">C Nakajima</name></author><author><name sortKey="Ohashi, K" uniqKey="Ohashi K">K Ohashi</name></author><author><name sortKey="Onuma, M" uniqKey="Onuma M">M Onuma</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xu, Jt" uniqKey="Xu J">JT Xu</name></author><author><name sortKey="Zhang, Zm" uniqKey="Zhang Z">ZM Zhang</name></author><author><name sortKey="Yin, Yb" uniqKey="Yin Y">YB Yin</name></author><author><name sortKey="Cui, Sj" uniqKey="Cui S">SJ Cui</name></author><author><name sortKey="Xu, Sz" uniqKey="Xu S">SZ Xu</name></author><author><name sortKey="Guo, Yy" uniqKey="Guo Y">YY Guo</name></author><author><name sortKey="Li, Jd" uniqKey="Li J">JD Li</name></author><author><name sortKey="Wang, Jl" uniqKey="Wang J">JL Wang</name></author><author><name sortKey="Liu, Xc" uniqKey="Liu X">XC Liu</name></author><author><name sortKey="Han, Lm" uniqKey="Han L">LM Han</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Poon, Llm" uniqKey="Poon L">LLM Poon</name></author><author><name sortKey="Leung, Csw" uniqKey="Leung C">CSW Leung</name></author><author><name sortKey="Chan, Kh" uniqKey="Chan K">KH Chan</name></author><author><name sortKey="Lee, Jhc" uniqKey="Lee J">JHC Lee</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name></author><author><name sortKey="Peiris, Jsm" uniqKey="Peiris J">JSM Peiris</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ito, M" uniqKey="Ito M">M Ito</name></author><author><name sortKey="Watanabe, M" uniqKey="Watanabe M">M Watanabe</name></author><author><name sortKey="Nakagawa, N" uniqKey="Nakagawa N">N Nakagawa</name></author><author><name sortKey="Okuno, Y" uniqKey="Okuno Y">Y Okuno</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Imai, M" uniqKey="Imai M">M Imai</name></author><author><name sortKey="Ninomiya, A" uniqKey="Ninomiya A">A Ninomiya</name></author><author><name sortKey="Minekawa, H" uniqKey="Minekawa H">H Minekawa</name></author><author><name sortKey="Notomi, T" uniqKey="Notomi T">T Notomi</name></author><author><name sortKey="Ishizaki, T" uniqKey="Ishizaki T">T Ishizaki</name></author><author><name sortKey="Tashiro, M" uniqKey="Tashiro M">M Tashiro</name></author><author><name sortKey="Odagiri, T" uniqKey="Odagiri T">T Odagiri</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Imai, M" uniqKey="Imai M">M Imai</name></author><author><name sortKey="Ninomiya, A" uniqKey="Ninomiya A">A Ninomiya</name></author><author><name sortKey="Minekawa, H" uniqKey="Minekawa H">H Minekawa</name></author><author><name sortKey="Notomi, T" uniqKey="Notomi T">T Notomi</name></author><author><name sortKey="Ishizaki, T" uniqKey="Ishizaki T">T Ishizaki</name></author><author><name sortKey="Tu, Pv" uniqKey="Tu P">PV Tu</name></author><author><name sortKey="Tien, Ntk" uniqKey="Tien N">NTK Tien</name></author><author><name sortKey="Tashiro, M" uniqKey="Tashiro M">M Tashiro</name></author><author><name sortKey="Odagiri, T" uniqKey="Odagiri T">T Odagiri</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ma, Xj" uniqKey="Ma X">XJ Ma</name></author><author><name sortKey="Shu, Yl" uniqKey="Shu Y">YL Shu</name></author><author><name sortKey="Nie, K" uniqKey="Nie K">K Nie</name></author><author><name sortKey="Qin, M" uniqKey="Qin M">M Qin</name></author><author><name sortKey="Wang, Dy" uniqKey="Wang D">DY Wang</name></author><author><name sortKey="Gao, Rb" uniqKey="Gao R">RB Gao</name></author><author><name sortKey="Wang, M" uniqKey="Wang M">M Wang</name></author><author><name sortKey="Wen, Ly" uniqKey="Wen L">LY Wen</name></author><author><name sortKey="Han, F" uniqKey="Han F">F Han</name></author><author><name sortKey="Zhou, Sm" uniqKey="Zhou S">SM Zhou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kubo, T" uniqKey="Kubo T">T Kubo</name></author><author><name sortKey="Agoh, M" uniqKey="Agoh M">M Agoh</name></author><author><name sortKey="Q Mai, Le" uniqKey="Q Mai L">Le Q-Mai</name></author><author><name sortKey="Fukushima, K" uniqKey="Fukushima K">K Fukushima</name></author><author><name sortKey="Nishimura, H" uniqKey="Nishimura H">H Nishimura</name></author><author><name sortKey="Yamaguchi, A" uniqKey="Yamaguchi A">A Yamaguchi</name></author><author><name sortKey="Hirano, M" uniqKey="Hirano M">M Hirano</name></author><author><name sortKey="Yoshikawa, A" uniqKey="Yoshikawa A">A Yoshikawa</name></author><author><name sortKey="Hasebe, Fi" uniqKey="Hasebe F">Fi Hasebe</name></author><author><name sortKey="Kohno, S" uniqKey="Kohno S">S Kohno</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Swayne, Ed" uniqKey="Swayne E">ED Swayne</name></author><author><name sortKey="Glisson, Rj" uniqKey="Glisson R">RJ Glisson</name></author><author><name sortKey="Jackwood, Mw" uniqKey="Jackwood M">MW Jackwood</name></author><author><name sortKey="Pearson, Ej" uniqKey="Pearson E">EJ Pearson</name></author><author><name sortKey="Reed, Mw" uniqKey="Reed M">MW Reed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Das, A" uniqKey="Das A">A Das</name></author><author><name sortKey="Spackman, E" uniqKey="Spackman E">E Spackman</name></author><author><name sortKey="Senne, D" uniqKey="Senne D">D Senne</name></author><author><name sortKey="Pedersen, J" uniqKey="Pedersen J">J Pedersen</name></author><author><name sortKey="Suarez, Dl" uniqKey="Suarez D">DL Suarez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tsukamoto, K" uniqKey="Tsukamoto K">K Tsukamoto</name></author><author><name sortKey="Ashizawa, H" uniqKey="Ashizawa H">H Ashizawa</name></author><author><name sortKey="Nakanishi, K" uniqKey="Nakanishi K">K Nakanishi</name></author><author><name sortKey="Kaji, N" uniqKey="Kaji N">N Kaji</name></author><author><name sortKey="Suzuki, K" uniqKey="Suzuki K">K Suzuki</name></author><author><name sortKey="Okamatsu, M" uniqKey="Okamatsu M">M Okamatsu</name></author><author><name sortKey="Yamaguchi, S" uniqKey="Yamaguchi S">S Yamaguchi</name></author><author><name sortKey="Mase, M" uniqKey="Mase M">M Mase</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Virol J</journal-id><journal-title-group><journal-title>Virology Journal</journal-title></journal-title-group><issn pub-type="epub">1743-422X</issn><publisher><publisher-name>BioMed Central</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">21729297</article-id><article-id pub-id-type="pmc">3154870</article-id><article-id pub-id-type="publisher-id">1743-422X-8-337</article-id><article-id pub-id-type="doi">10.1186/1743-422X-8-337</article-id><article-categories><subj-group subj-group-type="heading"><subject>Methodology</subject></subj-group></article-categories><title-group><article-title>Visual detection of H3 subtype avian influenza viruses by reverse transcription loop-mediated isothermal amplification assay</article-title></title-group><contrib-group><contrib contrib-type="author" id="A1"><name><surname>Peng</surname><given-names>Yi</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>yipengsky@yahoo.com</email></contrib><contrib contrib-type="author" corresp="yes" id="A2"><name><surname>Xie</surname><given-names>Zhixun</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>xiezhixun@126.com</email></contrib><contrib contrib-type="author" id="A3"><name><surname>Liu</surname><given-names>Jiabo</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>jiaboliu@163.com</email></contrib><contrib contrib-type="author" id="A4"><name><surname>Pang</surname><given-names>Yaoshan</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>pcw6246@163.com</email></contrib><contrib contrib-type="author" id="A5"><name><surname>Deng</surname><given-names>Xianwen</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>xianwendeng@yahoo.com.cn</email></contrib><contrib contrib-type="author" id="A6"><name><surname>Xie</surname><given-names>Zhiqin</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>xzqman2002@sina.com</email></contrib><contrib contrib-type="author" id="A7"><name><surname>Xie</surname><given-names>Liji</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>liji@yahoo.com.cn</email></contrib><contrib contrib-type="author" id="A8"><name><surname>Fan</surname><given-names>Qing</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>fanqing1224@yahoo.com.cn</email></contrib><contrib contrib-type="author" id="A9"><name><surname>Feng</surname><given-names>Jiaxun</given-names></name><xref ref-type="aff" rid="I3">3</xref><email>jxfeng001@163.com</email></contrib><contrib contrib-type="author" corresp="yes" id="A10"><name><surname>Khan</surname><given-names>Mazhar I</given-names></name><xref ref-type="aff" rid="I2">2</xref><email>mazhar.khan@uconn.edu</email></contrib></contrib-group><aff id="I1"><label>1</label>Department of Biotechnology, Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi 530001, China</aff><aff id="I2"><label>2</label>Department of Pathobiology and Veterinary Science, University of Connecticut, 61 North Eagleville Road Storrs, CT 06269-3089, USA</aff><aff id="I3"><label>3</label>Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China</aff><pub-date pub-type="collection"><year>2011</year></pub-date><pub-date pub-type="epub"><day>5</day><month>7</month><year>2011</year></pub-date><volume>8</volume><fpage>337</fpage><lpage>337</lpage><history><date date-type="received"><day>1</day><month>4</month><year>2011</year></date><date date-type="accepted"><day>5</day><month>7</month><year>2011</year></date></history><permissions><copyright-statement>Copyright ©2011 Peng et al; licensee BioMed Central Ltd.</copyright-statement><copyright-year>2011</copyright-year><copyright-holder>Peng et al; licensee BioMed Central Ltd.</copyright-holder><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/2.0"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/2.0">http://creativecommons.org/licenses/by/2.0</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><self-uri xlink:href="http://www.virologyj.com/content/8/1/337"></self-uri><abstract><sec><title>Background</title><p>Recent epidemiological investigation of different HA subtypes of avian influenza viruses (AIVs) shows that the H3 subtype is the most predominant among low pathogenic AIVs (LPAIVs), and the seasonal variations in isolation of H3 subtype AIVs are consistent with that of human H3 subtype influenza viruses. Consequently, the development of a rapid, simple, sensitive detection method for H3 subtype AIVs is required. The loop-mediated isothermal amplification (LAMP) assay is a simple, rapid, sensitive and cost-effective nucleic acid amplification method that does not require any specialized equipment.</p></sec><sec><title>Results</title><p>A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to detect the H3 subtype AIVs visually. Specific primer sets target the sequences of the hemagglutinin (HA) gene of H3 subtype AIVs were designed, and assay reaction conditions were optimized. The established assay was performed in a water bath for 50 minutes, and the amplification result was visualized directly as well as under ultraviolet (UV) light reflections. The detection limit of the RT-LAMP assay was 0.1pg total RNA of virus, which was one hundred-fold higher than that of RT-PCR. The results on specificity indicated that the assay had no cross-reactions with other subtype AIVs or avian respiratory pathogens. Furthermore, a total of 176 clinical samples collected from birds at the various live-bird markets (LBMs) were subjected to the H3-subtype-specific RT-LAMP (H3-RT-LAMP). Thirty-eight H3 subtype AIVs were identified from the 176 clinical samples that were consistent with that of virus isolation.</p></sec><sec><title>Conclusions</title><p>The newly developed H3-RT-LAMP assay is simple, sensitive, rapid and can identify H3 subtype AIVs visually. Consequently, it will be a very useful screening assay for the surveillance of H3 subtype AIVs in underequipped laboratories as well as in field conditions.</p></sec></abstract><kwd-group><kwd>Loop-mediated isothermal amplification</kwd><kwd>H3 subtype</kwd><kwd>avian influenza</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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