Some factors affecting the production, by cultured baby-hamster kidney cells, of BHK glycoprotein I which cross-reacts immunologically with Tamm-Horsfall glycoprotein.
Identifieur interne : 000269 ( Pmc/Corpus ); précédent : 000268; suivant : 000270Some factors affecting the production, by cultured baby-hamster kidney cells, of BHK glycoprotein I which cross-reacts immunologically with Tamm-Horsfall glycoprotein.
Auteurs : F J Bloomfield ; D R Dunstan ; C L Foster ; F. Serafini-Cessi ; R D MarshallSource :
- Biochemical Journal [ 0264-6021 ] ; 1977.
Abstract
Cultured baby-hamster kidney cells (BHK-21/C13), which are adapted to grow in suspension (strain 2P), roduce a glycoprotein, termed BHK glycoprotein I, which cross-reacts immunologically with hamster urinary (Tamm-Horsfall glycoprotein. BHK glycoprotein I was isolated in an electrophoretically (sodium dodecyl sulphate/polyacrylamide gel) homogeneous form by application of affinity chromatography to the medium in which cells had been cultured. Insolubilized anti-(Tamm-Horsfall glycoprotein immunoglobulin G) was used as the adsorbent. The amount of BHK glycoprotein I associated with the cultured cells was found by both radioimmunoassay and immunofluorescence to be related to the amount of Ca2+ in the medium and to the particular stage of the cell cycle. 5'-Nucleotidase was also shed by the cells into the culture medium in amounts related to the stage of the cell cycle. The turnover of hamster Tamm-Horsfall glycoprotein in vivo appeared to be considerably more rapid than can be accounted for by cell turnover. Hamster Tamm-Horsfall glycoprotein was shown to be ineffective in inhibiting agglutination of chicken erythrocytes caused by influenza virus.
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PubMed: 328011
PubMed Central: 1164756
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PMC:1164756Le document en format XML
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<author><name sortKey="Dunstan, D R" sort="Dunstan, D R" uniqKey="Dunstan D" first="D R" last="Dunstan">D R Dunstan</name>
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<author><name sortKey="Foster, C L" sort="Foster, C L" uniqKey="Foster C" first="C L" last="Foster">C L Foster</name>
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<author><name sortKey="Serafini Cessi, F" sort="Serafini Cessi, F" uniqKey="Serafini Cessi F" first="F" last="Serafini-Cessi">F. Serafini-Cessi</name>
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<series><title level="j">Biochemical Journal</title>
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<front><div type="abstract" xml:lang="en"><p>Cultured baby-hamster kidney cells (BHK-21/C13), which are adapted to grow in suspension (strain 2P), roduce a glycoprotein, termed BHK glycoprotein I, which cross-reacts immunologically with hamster urinary (Tamm-Horsfall glycoprotein. BHK glycoprotein I was isolated in an electrophoretically (sodium dodecyl sulphate/polyacrylamide gel) homogeneous form by application of affinity chromatography to the medium in which cells had been cultured. Insolubilized anti-(Tamm-Horsfall glycoprotein immunoglobulin G) was used as the adsorbent. The amount of BHK glycoprotein I associated with the cultured cells was found by both radioimmunoassay and immunofluorescence to be related to the amount of Ca2+ in the medium and to the particular stage of the cell cycle. 5'-Nucleotidase was also shed by the cells into the culture medium in amounts related to the stage of the cell cycle. The turnover of hamster Tamm-Horsfall glycoprotein in vivo appeared to be considerably more rapid than can be accounted for by cell turnover. Hamster Tamm-Horsfall glycoprotein was shown to be ineffective in inhibiting agglutination of chicken erythrocytes caused by influenza virus.</p>
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<front><journal-meta><journal-id journal-id-type="nlm-ta">Biochem J</journal-id>
<journal-title>Biochemical Journal</journal-title>
<issn pub-type="ppub">0264-6021</issn>
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<title-group><article-title>Some factors affecting the production, by cultured baby-hamster kidney cells, of BHK glycoprotein I which cross-reacts immunologically with Tamm-Horsfall glycoprotein.</article-title>
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<contrib-group><contrib contrib-type="author"><name><surname>Bloomfield</surname>
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<contrib contrib-type="author"><name><surname>Dunstan</surname>
<given-names>D R</given-names>
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<contrib contrib-type="author"><name><surname>Foster</surname>
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<pub-date pub-type="ppub"><day>15</day>
<month>4</month>
<year>1977</year>
</pub-date>
<volume>164</volume>
<issue>1</issue>
<fpage>41</fpage>
<lpage>51</lpage>
<abstract><p>Cultured baby-hamster kidney cells (BHK-21/C13), which are adapted to grow in suspension (strain 2P), roduce a glycoprotein, termed BHK glycoprotein I, which cross-reacts immunologically with hamster urinary (Tamm-Horsfall glycoprotein. BHK glycoprotein I was isolated in an electrophoretically (sodium dodecyl sulphate/polyacrylamide gel) homogeneous form by application of affinity chromatography to the medium in which cells had been cultured. Insolubilized anti-(Tamm-Horsfall glycoprotein immunoglobulin G) was used as the adsorbent. The amount of BHK glycoprotein I associated with the cultured cells was found by both radioimmunoassay and immunofluorescence to be related to the amount of Ca2+ in the medium and to the particular stage of the cell cycle. 5'-Nucleotidase was also shed by the cells into the culture medium in amounts related to the stage of the cell cycle. The turnover of hamster Tamm-Horsfall glycoprotein in vivo appeared to be considerably more rapid than can be accounted for by cell turnover. Hamster Tamm-Horsfall glycoprotein was shown to be ineffective in inhibiting agglutination of chicken erythrocytes caused by influenza virus.</p>
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