Trypsinized Human Group O Erythrocytes as an Alternative Hemagglutinating Agent for Japanese Encephalitis Virus
Identifieur interne : 000268 ( Pmc/Corpus ); précédent : 000267; suivant : 000269Trypsinized Human Group O Erythrocytes as an Alternative Hemagglutinating Agent for Japanese Encephalitis Virus
Auteurs : K. F. Shortridge ; L. Y. HuSource :
- Applied Microbiology [ 0003-6919 ] ; 1974.
Abstract
Trypsinized human group O erythrocytes were found to be a suitable alternative to gander cells in hemagglutination (HA) and hemagglutination inhibition (HAI) tests for Japanese encephalitis (JE) virus. In the HAI test, no cross-reactions against JE virus were observed with immune sera containing antibody to taxonomically related or unrelated viruses, with mouse brain antigen, or with nonantibody serum inhibitors; specific antibody rise could be detected in an immunized rabbit. Gander and trypsinized human group O cells gave comparable titers in the HAI test, but the latter were preferable since (i) they required less challenging HA antigen, being more sensitive to agglutination by JE virus, and (ii) all human and some animal sera investigated were devoid of natural agglutinins for these cells, thereby eliminating or reducing the need for prior adsorption with packed cells.
Url:
PubMed: 4856948
PubMed Central: 380111
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PMC:380111Le document en format XML
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<author><name sortKey="Shortridge, K F" sort="Shortridge, K F" uniqKey="Shortridge K" first="K. F." last="Shortridge">K. F. Shortridge</name>
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<author><name sortKey="Hu, L Y" sort="Hu, L Y" uniqKey="Hu L" first="L. Y." last="Hu">L. Y. Hu</name>
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<author><name sortKey="Hu, L Y" sort="Hu, L Y" uniqKey="Hu L" first="L. Y." last="Hu">L. Y. Hu</name>
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<series><title level="j">Applied Microbiology</title>
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<front><div type="abstract" xml:lang="en"><p>Trypsinized human group O erythrocytes were found to be a suitable alternative to gander cells in hemagglutination (HA) and hemagglutination inhibition (HAI) tests for Japanese encephalitis (JE) virus. In the HAI test, no cross-reactions against JE virus were observed with immune sera containing antibody to taxonomically related or unrelated viruses, with mouse brain antigen, or with nonantibody serum inhibitors; specific antibody rise could be detected in an immunized rabbit. Gander and trypsinized human group O cells gave comparable titers in the HAI test, but the latter were preferable since (i) they required less challenging HA antigen, being more sensitive to agglutination by JE virus, and (ii) all human and some animal sera investigated were devoid of natural agglutinins for these cells, thereby eliminating or reducing the need for prior adsorption with packed cells.</p>
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<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Appl Microbiol</journal-id>
<journal-title>Applied Microbiology</journal-title>
<issn pub-type="ppub">0003-6919</issn>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">4856948</article-id>
<article-id pub-id-type="pmc">380111</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Virology and Viral Immunology</subject>
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</article-categories>
<title-group><article-title>Trypsinized Human Group O Erythrocytes as an Alternative Hemagglutinating Agent for Japanese Encephalitis Virus</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Shortridge</surname>
<given-names>K. F.</given-names>
</name>
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<contrib contrib-type="author"><name><surname>Hu</surname>
<given-names>L. Y.</given-names>
</name>
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<aff id="af1">Department of Microbiology, University of Hong Kong, Queen Mary Hospital Compound, Hong Kong</aff>
<pub-date pub-type="ppub"><month>04</month>
<year>1974</year>
</pub-date>
<volume>27</volume>
<issue>4</issue>
<fpage>653</fpage>
<lpage>656</lpage>
<copyright-statement>Copyright © 1974 American Society for Microbiology</copyright-statement>
<abstract><p>Trypsinized human group O erythrocytes were found to be a suitable alternative to gander cells in hemagglutination (HA) and hemagglutination inhibition (HAI) tests for Japanese encephalitis (JE) virus. In the HAI test, no cross-reactions against JE virus were observed with immune sera containing antibody to taxonomically related or unrelated viruses, with mouse brain antigen, or with nonantibody serum inhibitors; specific antibody rise could be detected in an immunized rabbit. Gander and trypsinized human group O cells gave comparable titers in the HAI test, but the latter were preferable since (i) they required less challenging HA antigen, being more sensitive to agglutination by JE virus, and (ii) all human and some animal sera investigated were devoid of natural agglutinins for these cells, thereby eliminating or reducing the need for prior adsorption with packed cells.</p>
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