An influenza A live attenuated reassortant virus possessing three temperature-sensitive mutations in the PB2 polymerase gene rapidly loses temperature sensitivity following replication in hamsters
Identifieur interne : 000083 ( PascalFrancis/Corpus ); précédent : 000082; suivant : 000084An influenza A live attenuated reassortant virus possessing three temperature-sensitive mutations in the PB2 polymerase gene rapidly loses temperature sensitivity following replication in hamsters
Auteurs : B. R. Murphy ; EUN JU PARK ; P. Gottlieb ; K. SubbaraoSource :
- Vaccine [ 0264-410X ] ; 1997.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
The purpose of the present study was to produce an influenz A H2N2 donor virus from which an attenuating PB2 gene bearing three discrete temperature sensitive (ts) mutations could be readily transferred to currently epidemic influenza A HIN1 and H3N2 viruses via genetic reassortment. An influenza A transfectant virus was first produced that contained site-directed ts mutations at mino acids 112, 265, and 556 in the PB2 gene of influenza A/AA/60 virus origin in background of the other seven RNA segments from the influenza A/LA/87 (H3N2) virus. The A/LA/87 PB2 ts trans- fectant virus (clone 22Bl) was mated with the A/AA/60 (H2N2) wild type virus, and six H2N2 ts reassortants were obtained. One reassortant virus, clone 25A1, possessed the triple ts PB2 gene in the context of all seven other genes of homologus A/AA/60 virus origin in a background of the other seven RNA segments from the influenza A/LA/87 (H3N2) virus. The A/LA/87 PB2 ts transfectant virus (clone 22B1) was mated with the A/AA/60 (N2H2) wild type virus, and six H2N2 ts reassortants were obtained. One reassortant virus, clone 25A1, possessed the triplets PB2 gene in the context of all seven other genes of homologous A/AA/60 origin. Isolation of this reassortant permitted an examination of the contribution of the ts mutations present in a triple ts PB2 transfectant virus to its attenuation and phenotypic stability independent from an effect of the A/AA/60-A/LA/87 gene constellation on attenuation. It was found that the A/AA/60 triple ts reassortant virus was less ts, less attenuated, and less phenotypically stable than the A/ALA/87 triple ts transfectant virus from which it was derived. The A/AA/60 reassortant possessing the PB2 gene containing three introduced ts mutations underwent rapid and significant loss of its temperature sensitivity following replictaion in the lungs of immunocompetent hamsteers. This indicated that the A/AA/87 triple ts transfectant virus. It is likely that the instability of the ts phenotype exhibited by the A/AA/60 triple ts reassortant virus would not be acceptable for a vaccine to be used in humans. The implications of these findings for the usefulness of ts mutations as the sole attenuating mutation in influenza virus vaccines is discussed.
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Format Inist (serveur)
| NO : | PASCAL 97-0483720 INIST |
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| ET : | An influenza A live attenuated reassortant virus possessing three temperature-sensitive mutations in the PB2 polymerase gene rapidly loses temperature sensitivity following replication in hamsters |
| AU : | MURPHY (B. R.); EUN JU PARK; GOTTLIEB (P.); SUBBARAO (K.) |
| AF : | Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, NIH, 7 Center Drive, MSC 0720/Bethesda, MD 20892-0720/Etats-Unis (1 aut., 2 aut., 3 aut., 4 aut.) |
| DT : | Publication en série; Niveau analytique |
| SO : | Vaccine; ISSN 0264-410X; Coden VACCDE; Royaume-Uni; Da. 1997; Vol. 15; No. 12-13; Pp. 1372-1378; Bibl. 28 ref. |
| LA : | Anglais |
| EA : | The purpose of the present study was to produce an influenz A H2N2 donor virus from which an attenuating PB2 gene bearing three discrete temperature sensitive (ts) mutations could be readily transferred to currently epidemic influenza A HIN1 and H3N2 viruses via genetic reassortment. An influenza A transfectant virus was first produced that contained site-directed ts mutations at mino acids 112, 265, and 556 in the PB2 gene of influenza A/AA/60 virus origin in background of the other seven RNA segments from the influenza A/LA/87 (H3N2) virus. The A/LA/87 PB2 ts trans- fectant virus (clone 22Bl) was mated with the A/AA/60 (H2N2) wild type virus, and six H2N2 ts reassortants were obtained. One reassortant virus, clone 25A1, possessed the triple ts PB2 gene in the context of all seven other genes of homologus A/AA/60 virus origin in a background of the other seven RNA segments from the influenza A/LA/87 (H3N2) virus. The A/LA/87 PB2 ts transfectant virus (clone 22B1) was mated with the A/AA/60 (N2H2) wild type virus, and six H2N2 ts reassortants were obtained. One reassortant virus, clone 25A1, possessed the triplets PB2 gene in the context of all seven other genes of homologous A/AA/60 origin. Isolation of this reassortant permitted an examination of the contribution of the ts mutations present in a triple ts PB2 transfectant virus to its attenuation and phenotypic stability independent from an effect of the A/AA/60-A/LA/87 gene constellation on attenuation. It was found that the A/AA/60 triple ts reassortant virus was less ts, less attenuated, and less phenotypically stable than the A/ALA/87 triple ts transfectant virus from which it was derived. The A/AA/60 reassortant possessing the PB2 gene containing three introduced ts mutations underwent rapid and significant loss of its temperature sensitivity following replictaion in the lungs of immunocompetent hamsteers. This indicated that the A/AA/87 triple ts transfectant virus. It is likely that the instability of the ts phenotype exhibited by the A/AA/60 triple ts reassortant virus would not be acceptable for a vaccine to be used in humans. The implications of these findings for the usefulness of ts mutations as the sole attenuating mutation in influenza virus vaccines is discussed. |
| CC : | 002A05C07 |
| FD : | Influenzavirus A; Vaccin; Souche atténuée; Mutation thermosensible; RNA-directed RNA polymerase; Stabilité; Réversion; Gène PB2; Réassortiment génétique |
| FG : | Influenzavirus; Orthomyxoviridae; Virus; Nucleotidyltransferases; Transferases; Enzyme |
| ED : | Influenzavirus A; Vaccine; Attenuated strain; Temperature sensitive mutation; RNA-directed RNA polymerase; Stability; Reversion; Genetic reassortment |
| EG : | Influenzavirus; Orthomyxoviridae; Virus; Nucleotidyltransferases; Transferases; Enzyme |
| GD : | Stabilitaet; Reversion |
| SD : | Influenzavirus A; Vacuna; Cepa atenuada; Mutación termosensible; RNA-directed RNA polymerase; Estabilidad; Reversión |
| LO : | INIST-20289.354000068905530140 |
| ID : | 97-0483720 |
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Pascal:97-0483720Le document en format XML
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Murphy</name><affiliation><inist:fA14 i1="01"><s1>Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, NIH, 7 Center Drive, MSC 0720</s1><s2>Bethesda, MD 20892-0720</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Eun Ju Park" sort="Eun Ju Park" uniqKey="Eun Ju Park" last="Eun Ju Park">EUN JU PARK</name><affiliation><inist:fA14 i1="01"><s1>Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, NIH, 7 Center Drive, MSC 0720</s1><s2>Bethesda, MD 20892-0720</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Gottlieb, P" sort="Gottlieb, P" uniqKey="Gottlieb P" first="P." last="Gottlieb">P. 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Subbarao</name><affiliation><inist:fA14 i1="01"><s1>Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, NIH, 7 Center Drive, MSC 0720</s1><s2>Bethesda, MD 20892-0720</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">97-0483720</idno><date when="1997">1997</date><idno type="stanalyst">PASCAL 97-0483720 INIST</idno><idno type="RBID">Pascal:97-0483720</idno><idno type="wicri:Area/PascalFrancis/Corpus">000083</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">An influenza A live attenuated reassortant virus possessing three temperature-sensitive mutations in the PB2 polymerase gene rapidly loses temperature sensitivity following replication in hamsters</title><author><name sortKey="Murphy, B R" sort="Murphy, B R" uniqKey="Murphy B" first="B. R." last="Murphy">B. R. Murphy</name><affiliation><inist:fA14 i1="01"><s1>Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, NIH, 7 Center Drive, MSC 0720</s1><s2>Bethesda, MD 20892-0720</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Eun Ju Park" sort="Eun Ju Park" uniqKey="Eun Ju Park" last="Eun Ju Park">EUN JU PARK</name><affiliation><inist:fA14 i1="01"><s1>Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, NIH, 7 Center Drive, MSC 0720</s1><s2>Bethesda, MD 20892-0720</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Gottlieb, P" sort="Gottlieb, P" uniqKey="Gottlieb P" first="P." last="Gottlieb">P. Gottlieb</name><affiliation><inist:fA14 i1="01"><s1>Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, NIH, 7 Center Drive, MSC 0720</s1><s2>Bethesda, MD 20892-0720</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14></affiliation></author><author><name sortKey="Subbarao, K" sort="Subbarao, K" uniqKey="Subbarao K" first="K." last="Subbarao">K. Subbarao</name><affiliation><inist:fA14 i1="01"><s1>Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, NIH, 7 Center Drive, MSC 0720</s1><s2>Bethesda, MD 20892-0720</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14></affiliation></author></analytic><series><title level="j" type="main">Vaccine</title><title level="j" type="abbreviated">Vaccine</title><idno type="ISSN">0264-410X</idno><imprint><date when="1997">1997</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Vaccine</title><title level="j" type="abbreviated">Vaccine</title><idno type="ISSN">0264-410X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Attenuated strain</term><term>Genetic reassortment</term><term>Influenzavirus A</term><term>RNA-directed RNA polymerase</term><term>Reversion</term><term>Stability</term><term>Temperature sensitive mutation</term><term>Vaccine</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Influenzavirus A</term><term>Vaccin</term><term>Souche atténuée</term><term>Mutation thermosensible</term><term>RNA-directed RNA polymerase</term><term>Stabilité</term><term>Réversion</term><term>Gène PB2</term><term>Réassortiment génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The purpose of the present study was to produce an influenz A H2N2 donor virus from which an attenuating PB2 gene bearing three discrete temperature sensitive (ts) mutations could be readily transferred to currently epidemic influenza A HIN1 and H3N2 viruses via genetic reassortment. An influenza A transfectant virus was first produced that contained site-directed ts mutations at mino acids 112, 265, and 556 in the PB2 gene of influenza A/AA/60 virus origin in background of the other seven RNA segments from the influenza A/LA/87 (H3N2) virus. The A/LA/87 PB2 ts trans- fectant virus (clone 22Bl) was mated with the A/AA/60 (H2N2) wild type virus, and six H2N2 ts reassortants were obtained. One reassortant virus, clone 25A1, possessed the triple ts PB2 gene in the context of all seven other genes of homologus A/AA/60 virus origin in a background of the other seven RNA segments from the influenza A/LA/87 (H3N2) virus. The A/LA/87 PB2 ts transfectant virus (clone 22B1) was mated with the A/AA/60 (N2H2) wild type virus, and six H2N2 ts reassortants were obtained. One reassortant virus, clone 25A1, possessed the triplets PB2 gene in the context of all seven other genes of homologous A/AA/60 origin. Isolation of this reassortant permitted an examination of the contribution of the ts mutations present in a triple ts PB2 transfectant virus to its attenuation and phenotypic stability independent from an effect of the A/AA/60-A/LA/87 gene constellation on attenuation. It was found that the A/AA/60 triple ts reassortant virus was less ts, less attenuated, and less phenotypically stable than the A/ALA/87 triple ts transfectant virus from which it was derived. The A/AA/60 reassortant possessing the PB2 gene containing three introduced ts mutations underwent rapid and significant loss of its temperature sensitivity following replictaion in the lungs of immunocompetent hamsteers. This indicated that the A/AA/87 triple ts transfectant virus. It is likely that the instability of the ts phenotype exhibited by the A/AA/60 triple ts reassortant virus would not be acceptable for a vaccine to be used in humans. The implications of these findings for the usefulness of ts mutations as the sole attenuating mutation in influenza virus vaccines is discussed.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0264-410X</s0></fA01><fA02 i1="01"><s0>VACCDE</s0></fA02><fA03 i2="1"><s0>Vaccine</s0></fA03><fA05><s2>15</s2></fA05><fA06><s2>12-13</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>An influenza A live attenuated reassortant virus possessing three temperature-sensitive mutations in the PB2 polymerase gene rapidly loses temperature sensitivity following replication in hamsters</s1></fA08><fA11 i1="01" i2="1"><s1>MURPHY (B. R.)</s1></fA11><fA11 i1="02" i2="1"><s1>EUN JU PARK</s1></fA11><fA11 i1="03" i2="1"><s1>GOTTLIEB (P.)</s1></fA11><fA11 i1="04" i2="1"><s1>SUBBARAO (K.)</s1></fA11><fA14 i1="01"><s1>Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, NIH, 7 Center Drive, MSC 0720</s1><s2>Bethesda, MD 20892-0720</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></fA14><fA20><s1>1372-1378</s1></fA20><fA21><s1>1997</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>20289</s2><s5>354000068905530140</s5></fA43><fA44><s0>0000</s0><s1>© 1997 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>28 ref.</s0></fA45><fA47 i1="01" i2="1"><s0>97-0483720</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Vaccine</s0></fA64><fA66 i1="01"><s0>GBR</s0></fA66><fC01 i1="01" l="ENG"><s0>The purpose of the present study was to produce an influenz A H2N2 donor virus from which an attenuating PB2 gene bearing three discrete temperature sensitive (ts) mutations could be readily transferred to currently epidemic influenza A HIN1 and H3N2 viruses via genetic reassortment. An influenza A transfectant virus was first produced that contained site-directed ts mutations at mino acids 112, 265, and 556 in the PB2 gene of influenza A/AA/60 virus origin in background of the other seven RNA segments from the influenza A/LA/87 (H3N2) virus. The A/LA/87 PB2 ts trans- fectant virus (clone 22Bl) was mated with the A/AA/60 (H2N2) wild type virus, and six H2N2 ts reassortants were obtained. One reassortant virus, clone 25A1, possessed the triple ts PB2 gene in the context of all seven other genes of homologus A/AA/60 virus origin in a background of the other seven RNA segments from the influenza A/LA/87 (H3N2) virus. The A/LA/87 PB2 ts transfectant virus (clone 22B1) was mated with the A/AA/60 (N2H2) wild type virus, and six H2N2 ts reassortants were obtained. One reassortant virus, clone 25A1, possessed the triplets PB2 gene in the context of all seven other genes of homologous A/AA/60 origin. Isolation of this reassortant permitted an examination of the contribution of the ts mutations present in a triple ts PB2 transfectant virus to its attenuation and phenotypic stability independent from an effect of the A/AA/60-A/LA/87 gene constellation on attenuation. It was found that the A/AA/60 triple ts reassortant virus was less ts, less attenuated, and less phenotypically stable than the A/ALA/87 triple ts transfectant virus from which it was derived. The A/AA/60 reassortant possessing the PB2 gene containing three introduced ts mutations underwent rapid and significant loss of its temperature sensitivity following replictaion in the lungs of immunocompetent hamsteers. This indicated that the A/AA/87 triple ts transfectant virus. It is likely that the instability of the ts phenotype exhibited by the A/AA/60 triple ts reassortant virus would not be acceptable for a vaccine to be used in humans. The implications of these findings for the usefulness of ts mutations as the sole attenuating mutation in influenza virus vaccines is discussed.</s0></fC01><fC02 i1="01" i2="X"><s0>002A05C07</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Influenzavirus A</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Influenzavirus A</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Influenzavirus A</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Vaccin</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Vaccine</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Vacuna</s0><s5>02</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Souche atténuée</s0><s5>03</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Attenuated strain</s0><s5>03</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Cepa atenuada</s0><s5>03</s5></fC03><fC03 i1="04" i2="X" l="FRE"><s0>Mutation thermosensible</s0><s5>06</s5></fC03><fC03 i1="04" i2="X" l="ENG"><s0>Temperature sensitive mutation</s0><s5>06</s5></fC03><fC03 i1="04" i2="X" l="SPA"><s0>Mutación termosensible</s0><s5>06</s5></fC03><fC03 i1="05" i2="X" l="FRE"><s0>RNA-directed RNA polymerase</s0><s2>FE</s2><s5>07</s5></fC03><fC03 i1="05" i2="X" l="ENG"><s0>RNA-directed RNA polymerase</s0><s2>FE</s2><s5>07</s5></fC03><fC03 i1="05" i2="X" l="SPA"><s0>RNA-directed RNA polymerase</s0><s2>FE</s2><s5>07</s5></fC03><fC03 i1="06" i2="X" l="FRE"><s0>Stabilité</s0><s5>08</s5></fC03><fC03 i1="06" i2="X" l="ENG"><s0>Stability</s0><s5>08</s5></fC03><fC03 i1="06" i2="X" l="GER"><s0>Stabilitaet</s0><s5>08</s5></fC03><fC03 i1="06" i2="X" l="SPA"><s0>Estabilidad</s0><s5>08</s5></fC03><fC03 i1="07" i2="X" l="FRE"><s0>Réversion</s0><s5>09</s5></fC03><fC03 i1="07" i2="X" l="ENG"><s0>Reversion</s0><s5>09</s5></fC03><fC03 i1="07" i2="X" l="GER"><s0>Reversion</s0><s5>09</s5></fC03><fC03 i1="07" i2="X" l="SPA"><s0>Reversión</s0><s5>09</s5></fC03><fC03 i1="08" i2="X" l="FRE"><s0>Gène PB2</s0><s4>INC</s4><s5>79</s5></fC03><fC03 i1="09" i2="X" l="FRE"><s0>Réassortiment génétique</s0><s4>CD</s4><s5>99</s5></fC03><fC03 i1="09" i2="X" l="ENG"><s0>Genetic reassortment</s0><s4>CD</s4><s5>99</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Influenzavirus</s0><s2>NW</s2></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Influenzavirus</s0><s2>NW</s2></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Influenzavirus</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="FRE"><s0>Orthomyxoviridae</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="ENG"><s0>Orthomyxoviridae</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="SPA"><s0>Orthomyxoviridae</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="FRE"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="ENG"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="SPA"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="04" i2="X" l="FRE"><s0>Nucleotidyltransferases</s0><s2>FE</s2></fC07><fC07 i1="04" i2="X" l="ENG"><s0>Nucleotidyltransferases</s0><s2>FE</s2></fC07><fC07 i1="04" i2="X" l="SPA"><s0>Nucleotidyltransferases</s0><s2>FE</s2></fC07><fC07 i1="05" i2="X" l="FRE"><s0>Transferases</s0><s2>FE</s2></fC07><fC07 i1="05" i2="X" l="ENG"><s0>Transferases</s0><s2>FE</s2></fC07><fC07 i1="05" i2="X" l="SPA"><s0>Transferases</s0><s2>FE</s2></fC07><fC07 i1="06" i2="X" l="FRE"><s0>Enzyme</s0></fC07><fC07 i1="06" i2="X" l="ENG"><s0>Enzyme</s0></fC07><fC07 i1="06" i2="X" l="SPA"><s0>Enzima</s0></fC07><fN21><s1>293</s1></fN21></pA></standard><server><NO>PASCAL 97-0483720 INIST</NO><ET>An influenza A live attenuated reassortant virus possessing three temperature-sensitive mutations in the PB2 polymerase gene rapidly loses temperature sensitivity following replication in hamsters</ET><AU>MURPHY (B. R.); EUN JU PARK; GOTTLIEB (P.); SUBBARAO (K.)</AU><AF>Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, NIH, 7 Center Drive, MSC 0720/Bethesda, MD 20892-0720/Etats-Unis (1 aut., 2 aut., 3 aut., 4 aut.)</AF><DT>Publication en série; Niveau analytique</DT><SO>Vaccine; ISSN 0264-410X; Coden VACCDE; Royaume-Uni; Da. 1997; Vol. 15; No. 12-13; Pp. 1372-1378; Bibl. 28 ref.</SO><LA>Anglais</LA><EA>The purpose of the present study was to produce an influenz A H2N2 donor virus from which an attenuating PB2 gene bearing three discrete temperature sensitive (ts) mutations could be readily transferred to currently epidemic influenza A HIN1 and H3N2 viruses via genetic reassortment. An influenza A transfectant virus was first produced that contained site-directed ts mutations at mino acids 112, 265, and 556 in the PB2 gene of influenza A/AA/60 virus origin in background of the other seven RNA segments from the influenza A/LA/87 (H3N2) virus. The A/LA/87 PB2 ts trans- fectant virus (clone 22Bl) was mated with the A/AA/60 (H2N2) wild type virus, and six H2N2 ts reassortants were obtained. One reassortant virus, clone 25A1, possessed the triple ts PB2 gene in the context of all seven other genes of homologus A/AA/60 virus origin in a background of the other seven RNA segments from the influenza A/LA/87 (H3N2) virus. The A/LA/87 PB2 ts transfectant virus (clone 22B1) was mated with the A/AA/60 (N2H2) wild type virus, and six H2N2 ts reassortants were obtained. One reassortant virus, clone 25A1, possessed the triplets PB2 gene in the context of all seven other genes of homologous A/AA/60 origin. Isolation of this reassortant permitted an examination of the contribution of the ts mutations present in a triple ts PB2 transfectant virus to its attenuation and phenotypic stability independent from an effect of the A/AA/60-A/LA/87 gene constellation on attenuation. It was found that the A/AA/60 triple ts reassortant virus was less ts, less attenuated, and less phenotypically stable than the A/ALA/87 triple ts transfectant virus from which it was derived. The A/AA/60 reassortant possessing the PB2 gene containing three introduced ts mutations underwent rapid and significant loss of its temperature sensitivity following replictaion in the lungs of immunocompetent hamsteers. This indicated that the A/AA/87 triple ts transfectant virus. It is likely that the instability of the ts phenotype exhibited by the A/AA/60 triple ts reassortant virus would not be acceptable for a vaccine to be used in humans. The implications of these findings for the usefulness of ts mutations as the sole attenuating mutation in influenza virus vaccines is discussed.</EA><CC>002A05C07</CC><FD>Influenzavirus A; Vaccin; Souche atténuée; Mutation thermosensible; RNA-directed RNA polymerase; Stabilité; Réversion; Gène PB2; Réassortiment génétique</FD><FG>Influenzavirus; Orthomyxoviridae; Virus; Nucleotidyltransferases; Transferases; Enzyme</FG><ED>Influenzavirus A; Vaccine; Attenuated strain; Temperature sensitive mutation; RNA-directed RNA polymerase; Stability; Reversion; Genetic reassortment</ED><EG>Influenzavirus; Orthomyxoviridae; Virus; Nucleotidyltransferases; Transferases; Enzyme</EG><GD>Stabilitaet; Reversion</GD><SD>Influenzavirus A; Vacuna; Cepa atenuada; Mutación termosensible; RNA-directed RNA polymerase; Estabilidad; Reversión</SD><LO>INIST-20289.354000068905530140</LO><ID>97-0483720</ID></server></inist></record>Pour manipuler ce document sous Unix (Dilib)
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