A Fusion Protein Based on the Second Subunit of Hemagglutinin of Influenza A/H2N2 Viruses Provides Cross Immunity.
Identifieur interne : 000C95 ( Ncbi/Checkpoint ); précédent : 000C94; suivant : 000C96A Fusion Protein Based on the Second Subunit of Hemagglutinin of Influenza A/H2N2 Viruses Provides Cross Immunity.
Auteurs : L A Stepanova [Russie] ; M V Sergeeva [Russie] ; M A Shuklina [Russie] ; A A Shaldzhyan [Russie] ; M V Potapchuk [Russie] ; A V Korotkov [Russie] ; L M Tsybalova [Russie]Source :
- Acta naturae [ 2075-8251 ]
Abstract
Conserved fragments of the second subunit of hemagglutinin (HA2) are of great interest for the design of vaccine constructs that can provide protective immunity against influenza A viruses of different subtypes. A recombinant fusion protein, FlgMH, was constructed on the basis of flagellin and a highly conserved HA2 fragment (35-107) of influenza viruses of the subtype A/H2N2, containing B cell, CD4+ T cell, and CD8+ T cell epitopes. The native conformation of the HA2 fragment was partially preserved upon its attachment to the C-terminus of flagellin within the recombinant fusion protein FlgMH. FlgMH was shown to stimulate a mixed Th1/Th2 response of cross-reactive antibodies, which bind to influenza viruses of the first phylogenetic group (H1, H2, H5), to the target sequence as well as the induction of specific cytotoxic T cells (CD3+CD8+IFNγ+). Immunization with the recombinant protein protected animals from a lethal influenza infection. The developed FlgMH protein is a promising agent that may be included in an influenza vaccine with a wide spectrum of action which will be able to stimulate the T and B cell immune responses.
PubMed: 27437146
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000047
- to stream PubMed, to step Curation: 000047
- to stream PubMed, to step Checkpoint: 000582
- to stream Ncbi, to step Merge: 000C95
- to stream Ncbi, to step Curation: 000C95
Links to Exploration step
pubmed:27437146Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A Fusion Protein Based on the Second Subunit of Hemagglutinin of Influenza A/H2N2 Viruses Provides Cross Immunity.</title><author><name sortKey="Stepanova, L A" sort="Stepanova, L A" uniqKey="Stepanova L" first="L A" last="Stepanova">L A Stepanova</name><affiliation wicri:level="1"><nlm:affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg</wicri:regionArea><wicri:noRegion>St. Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Sergeeva, M V" sort="Sergeeva, M V" uniqKey="Sergeeva M" first="M V" last="Sergeeva">M V Sergeeva</name><affiliation wicri:level="1"><nlm:affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg</wicri:regionArea><wicri:noRegion>St. Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Shuklina, M A" sort="Shuklina, M A" uniqKey="Shuklina M" first="M A" last="Shuklina">M A Shuklina</name><affiliation wicri:level="1"><nlm:affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg</wicri:regionArea><wicri:noRegion>St. Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Shaldzhyan, A A" sort="Shaldzhyan, A A" uniqKey="Shaldzhyan A" first="A A" last="Shaldzhyan">A A Shaldzhyan</name><affiliation wicri:level="1"><nlm:affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg</wicri:regionArea><wicri:noRegion>St. Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Potapchuk, M V" sort="Potapchuk, M V" uniqKey="Potapchuk M" first="M V" last="Potapchuk">M V Potapchuk</name><affiliation wicri:level="1"><nlm:affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg</wicri:regionArea><wicri:noRegion>St. Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Korotkov, A V" sort="Korotkov, A V" uniqKey="Korotkov A" first="A V" last="Korotkov">A V Korotkov</name><affiliation wicri:level="1"><nlm:affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg</wicri:regionArea><wicri:noRegion>St. Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Tsybalova, L M" sort="Tsybalova, L M" uniqKey="Tsybalova L" first="L M" last="Tsybalova">L M Tsybalova</name><affiliation wicri:level="1"><nlm:affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg</wicri:regionArea><wicri:noRegion>St. Petersburg</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="????"><PubDate><MedlineDate>2016 Apr-Jun</MedlineDate></PubDate></date><idno type="RBID">pubmed:27437146</idno><idno type="pmid">27437146</idno><idno type="wicri:Area/PubMed/Corpus">000047</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000047</idno><idno type="wicri:Area/PubMed/Curation">000047</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000047</idno><idno type="wicri:Area/PubMed/Checkpoint">000582</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000582</idno><idno type="wicri:Area/Ncbi/Merge">000C95</idno><idno type="wicri:Area/Ncbi/Curation">000C95</idno><idno type="wicri:Area/Ncbi/Checkpoint">000C95</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A Fusion Protein Based on the Second Subunit of Hemagglutinin of Influenza A/H2N2 Viruses Provides Cross Immunity.</title><author><name sortKey="Stepanova, L A" sort="Stepanova, L A" uniqKey="Stepanova L" first="L A" last="Stepanova">L A Stepanova</name><affiliation wicri:level="1"><nlm:affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg</wicri:regionArea><wicri:noRegion>St. Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Sergeeva, M V" sort="Sergeeva, M V" uniqKey="Sergeeva M" first="M V" last="Sergeeva">M V Sergeeva</name><affiliation wicri:level="1"><nlm:affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg</wicri:regionArea><wicri:noRegion>St. Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Shuklina, M A" sort="Shuklina, M A" uniqKey="Shuklina M" first="M A" last="Shuklina">M A Shuklina</name><affiliation wicri:level="1"><nlm:affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg</wicri:regionArea><wicri:noRegion>St. Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Shaldzhyan, A A" sort="Shaldzhyan, A A" uniqKey="Shaldzhyan A" first="A A" last="Shaldzhyan">A A Shaldzhyan</name><affiliation wicri:level="1"><nlm:affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg</wicri:regionArea><wicri:noRegion>St. Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Potapchuk, M V" sort="Potapchuk, M V" uniqKey="Potapchuk M" first="M V" last="Potapchuk">M V Potapchuk</name><affiliation wicri:level="1"><nlm:affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg</wicri:regionArea><wicri:noRegion>St. Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Korotkov, A V" sort="Korotkov, A V" uniqKey="Korotkov A" first="A V" last="Korotkov">A V Korotkov</name><affiliation wicri:level="1"><nlm:affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg</wicri:regionArea><wicri:noRegion>St. Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Tsybalova, L M" sort="Tsybalova, L M" uniqKey="Tsybalova L" first="L M" last="Tsybalova">L M Tsybalova</name><affiliation wicri:level="1"><nlm:affiliation>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Research Institute of Influenza, Prof. Popova Str. 15/17, 197376, St. Petersburg</wicri:regionArea><wicri:noRegion>St. Petersburg</wicri:noRegion></affiliation></author></analytic><series><title level="j">Acta naturae</title><idno type="ISSN">2075-8251</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Conserved fragments of the second subunit of hemagglutinin (HA2) are of great interest for the design of vaccine constructs that can provide protective immunity against influenza A viruses of different subtypes. A recombinant fusion protein, FlgMH, was constructed on the basis of flagellin and a highly conserved HA2 fragment (35-107) of influenza viruses of the subtype A/H2N2, containing B cell, CD4+ T cell, and CD8+ T cell epitopes. The native conformation of the HA2 fragment was partially preserved upon its attachment to the C-terminus of flagellin within the recombinant fusion protein FlgMH. FlgMH was shown to stimulate a mixed Th1/Th2 response of cross-reactive antibodies, which bind to influenza viruses of the first phylogenetic group (H1, H2, H5), to the target sequence as well as the induction of specific cytotoxic T cells (CD3+CD8+IFNγ+). Immunization with the recombinant protein protected animals from a lethal influenza infection. The developed FlgMH protein is a promising agent that may be included in an influenza vaccine with a wide spectrum of action which will be able to stimulate the T and B cell immune responses. </div></front></TEI><affiliations><list><country><li>Russie</li></country></list><tree><country name="Russie"><noRegion><name sortKey="Stepanova, L A" sort="Stepanova, L A" uniqKey="Stepanova L" first="L A" last="Stepanova">L A Stepanova</name></noRegion><name sortKey="Korotkov, A V" sort="Korotkov, A V" uniqKey="Korotkov A" first="A V" last="Korotkov">A V Korotkov</name><name sortKey="Potapchuk, M V" sort="Potapchuk, M V" uniqKey="Potapchuk M" first="M V" last="Potapchuk">M V Potapchuk</name><name sortKey="Sergeeva, M V" sort="Sergeeva, M V" uniqKey="Sergeeva M" first="M V" last="Sergeeva">M V Sergeeva</name><name sortKey="Shaldzhyan, A A" sort="Shaldzhyan, A A" uniqKey="Shaldzhyan A" first="A A" last="Shaldzhyan">A A Shaldzhyan</name><name sortKey="Shuklina, M A" sort="Shuklina, M A" uniqKey="Shuklina M" first="M A" last="Shuklina">M A Shuklina</name><name sortKey="Tsybalova, L M" sort="Tsybalova, L M" uniqKey="Tsybalova L" first="L M" last="Tsybalova">L M Tsybalova</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Ncbi/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000C95 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd -nk 000C95 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Ncbi
|étape= Checkpoint
|type= RBID
|clé= pubmed:27437146
|texte= A Fusion Protein Based on the Second Subunit of Hemagglutinin of Influenza A/H2N2 Viruses Provides Cross Immunity.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/RBID.i -Sk "pubmed:27437146" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a H2N2V1
| This area was generated with Dilib version V0.6.33. |  |